Alloimmune Responses Of Humanized Mice To Human Pluripotent Stem Cell Therapeutics, 2017 Stanford University
Alloimmune Responses Of Humanized Mice To Human Pluripotent Stem Cell Therapeutics, Nigel G. Kooreman, Michael A. Brehm, Dale L. Greiner, Leonard D. Shultz, Joseph C. Wu
Open Access Articles
There is growing interest in using embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) derivatives for tissue regeneration. However, an increased understanding of human immune responses to stem cell-derived allografts is necessary for maintaining long-term graft persistence. To model this alloimmunity, humanized mice engrafted with human hematopoietic and immune cells could prove to be useful. In this study, an in-depth analysis of graft-infiltrating human lymphocytes and splenocytes revealed that humanized mice incompletely model human immune responses toward allogeneic stem cells and their derivatives. Furthermore, using an "allogenized" mouse model, we show the feasibility of reconstituting immunodeficient mice with ...
Germ-Line Gene Editing And Congressional Reaction In Context: Learning From Almost 50 Years Of Congressional Reactions To Biomedical Breakthroughs, Russell A. Spivak, J.D., I. Glenn Cohen, J.D., Eli Y. Adashi, M.D., M.S.
Journal of Law and Health
On December 18, 2015, President Obama signed into law a policy rider forestalling the therapeutic modification of the human germ line. The rider, motivated by the science’s potential unethical ends, is only the most recent instance in which the legislature cut short the ongoing national conversation on the acceptability of a developing science. This essay offers historical perspective on what bills were proposed and passed surrounding four other then-developing scientific breakthroughs—Recombinant DNA, in vitro fertilization, Cloning, Stem Cells—to better analyze how Congress is, and should, regulate this exciting and promising science.
Distortion In Body Schema: The Influence Of Body Fat And Mass On Perceptions Of Personal Size, Katarina Ann Ferrucci
Senior Projects Spring 2017
Obesity has been linked with a myriad of negative outcomes for both physical and mental health including feeding and eating disorders and cognitive impairments that affect perception of body size. Understanding the cognitive mechanisms and physiological factors that contribute to perception of body size may help us to comprehend how obesity impacts the construction and development of one’s mental body representations. Previous research by Scarpina, Castelnuovo, and Molinari (2014) suggests that, compared to those with a normal Body Mass Index, individuals with a BMI greater than 30 (obese) not only inaccurately estimate tactile and mental distances on their own ...
Identification Of Barriers To Organ Donation: A Primary Care Interventional Approach, Brittany L. Rocque Msc
Family Medicine Clerkship Student Projects
Organ transplantation provides a potentially life saving intervention for patient with end-organ failure as well as substantial cost-benefit with relation to Medicare expenditure. Despite the advantages of transplantation, deceased organ donors are in short supply and many patients die or lose quality years while awaiting transplantation. Registered organ donor rates are relatively low in our opt-in system being 52% nationwide and only 44% in Vermont, which is below the >50% per state goal. Non-organ donor patients were given and informational brochure and surveyed regarding their attitudes and beliefs toward organ donation.
Seeing The Invisible: Revealing Atrial Ablation Lesions Using Hyperspectral Imaging Approach, 2016 George Washington University
Seeing The Invisible: Revealing Atrial Ablation Lesions Using Hyperspectral Imaging Approach, Narine Muselimyan, Luther Swift, Huda Asfour, Tigran Chahbazian, Ramesh Mazhari, Marco Mercader, Narine Sarvazyan
Pharmacology and Physiology Faculty Publications
Currently, there are limited means for high-resolution monitoring of tissue injury during radiofrequency ablation procedures.
To develop the next generation of visualization catheters that can reveal irreversible atrial muscle damage caused by ablation and identify viability gaps between the lesions.
Radiofrequency lesions were placed on the endocardial surfaces of excised human and bovine atria and left ventricles of blood perfused rat hearts. Tissue was illuminated with 365nm light and a series of images were acquired from individual spectral bands within 420-720nm range. By extracting spectral profiles of individual pixels and spectral unmixing, the relative contribution of ablated ...
Transforming Body, 2016 Claremont Colleges
Transforming Body, Angelina Prendergast
The STEAM Journal
This is a reflection about the use of balls for body therapy.
Tissue Compartmentalization And Tropism Of Hiv-1: A Dissertation, 2016 University of Massachusetts Medical School
Tissue Compartmentalization And Tropism Of Hiv-1: A Dissertation, Robin L. Brese
GSBS Dissertations and Theses
Despite the development of effective antiretroviral treatments, there is still no cure for HIV-1. Major barriers to HIV-1 eradication include the diversity of intrapatient viral quasispecies and the establishment of reservoirs in tissue sanctuary sites. A better understanding of these populations is required for targeted treatments. While previous studies have examined the relationship between brain and blood or immune tissues, few have looked at and compared the properties of viruses from other tissue compartments. In this study, 75 full length HIV-1 envelopes were isolated from the frontal lobe, occipital lobe, parietal lobe, colon, lung, and lymph node of an HIV-1 ...
The Role Of Osteocyte Estrogen Receptor Beta (Erβ) In Regulating The Skeletal Response To Mechanical Loading, Julia P. Townsend, Russell P. Main
The Summer Undergraduate Research Fellowship (SURF) Symposium
Estrogen’s biological functions are mediated by estrogen binding to estrogen receptors (ER). Understanding what role both ERα and ERβ have in bone maintenance and formation can contribute to possible treatment of osteoporosis. This study examined osteocyte specific deletion of ERβ in mice. The cross of ERβ-floxed mice with DMP1-8kb-Cre mice provided both experimental knockout mice as well as littermate control mice. At 24 weeks of age the left tibiae of all mice were mechanically loaded five days per week for two weeks to induce bone formation. Analysis of cortical bone was conducted using microcomputed tomography (microCT) to measure load-induced ...
Unravelling The Subfields Of The Hippocampal Head Using 7-Tesla Structural Mri, 2016 The University of Western Ontario
Unravelling The Subfields Of The Hippocampal Head Using 7-Tesla Structural Mri, Jordan M. K. Dekraker
Electronic Thesis and Dissertation Repository
Probing the functions of human hippocampal subfields is a promising area of research in cognitive neuroscience. However, defining subfield borders in Magnetic Resonance Imaging (MRI) is challenging. Here, we present a user-guided, semi-automated protocol for segmenting hippocampal subfields on T2-weighted images obtained with 7-Tesla MRI. The protocol takes advantage of extant knowledge about regularities in hippocampal morphology and ontogeny that have not been systematically considered in prior related work. An image feature known as the hippocampal ‘dark band’ facilitates tracking of subfield continuities, allowing for unfolding and segmentation of convoluted hippocampal tissue. Initial results suggest that this protocol offers sufficient ...
Angioedema: Adverse Reaction From Ace-Inhibitors, 2016 Otterbein University
Angioedema: Adverse Reaction From Ace-Inhibitors, Jennifer L. Hawkins
Master of Science in Nursing (MSN) Student Scholarship
Angiotensin-converting enzyme inhibitor (ACEI) medications are one of the leading causes of angioedema in the United States, also known as ACEI-RA (Chan & Soliman, 2015). ACEI medications are frequently prescribed to help manage diseases, such as hypertension and congestive heart failure, and include, but are not limited to lisinopril and enalapril, with lisinopril being the most commonly prescribed at this time (Chan & Soliman, 2015). In addition, ACEIs are used to help prevent myocardial infarction, diabetic neuropathy, and a cerebrovascular accident (Chua, Ignaszewski, & Schwenger, 2011). There are several risk factors for developing ACEI-RA, with African-American females being the greatest at risk group ...
Spectral Changes Caused By Radiofrequency Ablation Of Cardiac Tissue, 2016 George Washington University
Spectral Changes Caused By Radiofrequency Ablation Of Cardiac Tissue, Mohammed Aljishi, Huda Asfour, Luther Swift, Narine Muselimyan, Marco A. Mercader, Narine Sarvazyan
GW Research Days 2016 - Present
New diagnostic catheters can be developed by delivering and acquiring light through a small fiberoptic bundle. This can provide a useful real time feedback guidance to observe tissue damage caused by thermal injury used to treat cardiac arrhythmias. Yet, little is known about the exact spectral changes caused by radiofrequency ablation (RFA) in different types of cardiac tissue. We hypothesized that the most sensitive optical ranges for characterizing thermal injury can be revealed by comparing spectral information from different areas of the heart before and after RF ablation. Freshly excised porcine hearts were used to acquire and analyze excitation emission ...
Bilayered, Non-Cross-Linked Collagen Matrix For Regeneration Of Facial Defects After Skin Cancer Removal: A New Perspective For Biomaterial-Based Tissue Reconstruction, 2016 George Washington University
Bilayered, Non-Cross-Linked Collagen Matrix For Regeneration Of Facial Defects After Skin Cancer Removal: A New Perspective For Biomaterial-Based Tissue Reconstruction, Shahram Ghanaat, Adorjan Kovacs, Mike Barbeck, Jonas Lorenz, Anna Teiler, Nader Sadeghi, Charles Kirkpatrick, Robert Sader
Surgery Faculty Publications
Classically skin defects are covered by split thickness skin grafts or by means of local or regional skin flaps. In the presented case series for the first time a bilayered, non-crossed-linked collagen matrix has been used in an off-label fashion in order to reconstruct facial skin defects following different types of skin cancer resection. The material is of porcine origin and consists of a spongy and a compact layer. The ratio of the two layers is 1:3 in favour of the spongy layer. The aim of the study was to investigate the potential of this matrix for skin regeneration ...
Effects On Muscle Tissue Remodeling And Lipid Metabolism In Muscle Tissue From Adult Patients With Polymyositis Or Dermatomyositis Treated With Immunosuppressive Agents., 2016 George Washington University
Effects On Muscle Tissue Remodeling And Lipid Metabolism In Muscle Tissue From Adult Patients With Polymyositis Or Dermatomyositis Treated With Immunosuppressive Agents., Ingela Loell, Joan Raouf, Yi-Wen Chen, Rongye Shi, Inger Nennesmo, Helene Alexanderson, Maryam Dastmalchi, Kanneboyina Nagaraju, Marina Korotkova, Ingrid E Lundberg
Genomics and Precision Medicine Faculty Publications
BACKGROUND: Polymyositis (PM) and dermatomyositis (DM) are autoimmune muscle diseases, conventionally treated with high doses of glucocorticoids in combination with immunosuppressive drugs. Treatment is often dissatisfying, with persisting muscle impairment. We aimed to investigate molecular mechanisms that might contribute to the persisting muscle impairment despite immunosuppressive treatment in adult patients with PM or DM using gene expression profiling of repeated muscle biopsies.
METHODS: Paired skeletal muscle biopsies from six newly diagnosed adult patients with DM or PM taken before and after conventional immunosuppressive treatment were examined by gene expression microarray analysis. Selected genes that displayed changes in expression were analyzed ...
Viewing The Extracellular Matrix: An Imaging Method For Tissue Engineering, 2015 Purdue University
Viewing The Extracellular Matrix: An Imaging Method For Tissue Engineering, Michael Drakopoulos, Sarah Calve
The Summer Undergraduate Research Fellowship (SURF) Symposium
The field of regenerative medicine seeks to create replacement tissues and organs, both to repair deficiencies in biological function and to treat structural damage caused by injury. Scaffoldings mimicking extracellular matrix (ECM), the structure to which cells attach to form tissues, have been developed from synthetic polymers and also been prepared by decellularizing adult tissue. However, the structure of ECM undergoes significant remodeling during natural tissue repair, suggesting that ECM-replacement constructs that mirror developing tissues may promote better regeneration than those modeled on adult tissues. This work investigated the effectiveness of a method of viewing the extracellular matrix of developing ...
Thymic Involution Perturbs Negative Selection And Leads To Chronic Inflammation, 2015 University of North Texas Health Science Center at Fort Worth
Thymic Involution Perturbs Negative Selection And Leads To Chronic Inflammation, Brandon D. Coder B.S.
Theses and Dissertations
The ubiquitous presence of chronic low-level pro-inflammatory factors in elderly individuals (termed inflammaging) is a significant risk factor for morbidity and mortality. The etiology of inflammaging is largely unknown. Recent evidence has identified the persistent activation of immune cells, thought to arise from latent viral infections, as key contributors towards the development of a chronic inflammatory environment. However, the contribution of autoreactive T cells towards the development of inflammaging has yet to be investigated. Another pervasive feature of the aging process is the age-related involution of the thymus gland, which has been linked with a predisposition toward developing autoimmunity. In ...
Tiki_Goddess On Thorlabs Digital Slide Imager (2x Zoom Down, Cropped), 2015 M.D. Anderson Cancer Center
Tiki_Goddess On Thorlabs Digital Slide Imager (2x Zoom Down, Cropped), George Mcnamara
Tiki_Goddess acquired on ThorLabs Digital Slide Imager (2x zoom down, cropped to fit). See also other Tiki_Goddess slide images on my bepress web site. I am posting the 2x zoom scan here after row stitching in MetaMorph, generating two stacks-->montages (about half length each), and doing final merge in Adobe Photoshop CS6. This image is cropped to just fit Tiki_Goddess itself (mouse tissue section, Masson trichrome stained).
TIFF image saved with LZW lossless compression.
See also the uncropped "full size" scan at
which is UNcropped, so is larger than this
Tiki_Goddess On Thorlabs Digital Slide Imager (2x Zoom Down, Entire Slide), 2015 M.D. Anderson Cancer Center
Tiki_Goddess On Thorlabs Digital Slide Imager (2x Zoom Down, Entire Slide), George Mcnamara
Tiki_Goddess on ThorLabs Digital Slide Imager (2x zoom down). See also other Tiki_Goddess slide images on my bepress web site. I am posting the 2x zoom scan here after row stitching in MetaMorph, generating two stacks-->montages (about half length each), and doing final merge in Adobe Photoshop CS6.
TIFF image saved with LZW lossless compression.
is UNcropped, so is larger than the
http://works.bepress.com/gmcnamara/70 cropped version.
Tiki_Goddess Olympus NanoZoomer
Tiki_Goddess On Thorlabs Digital Slide Imager (4x Zoom Down), 2015 M.D. Anderson Cancer Center
Tiki_Goddess On Thorlabs Digital Slide Imager (4x Zoom Down), George Mcnamara
Tiki_Goddess on ThorLabs Digital Slide Imager (4x zoom down), gamma 1.5. See also other Tiki_Goddess slide images on my bepress web site. I am posting the 4x zoom down image here because the full resolution and 2x zoom scans are too big for me to easily montage automatically in MetaMorph and way too much hassle to do manually in Adobe Photoshop.
Pathological Effects Of Repeated Concussive Tbi In Mouse Models: Periventricular Damage And Ventriculomegaly, 2015 University of Connecticut - Storrs
Pathological Effects Of Repeated Concussive Tbi In Mouse Models: Periventricular Damage And Ventriculomegaly, Richard H. Wolferz Jr.
Honors Scholar Theses
Repeated concussive traumatic brain injury (rcTBI) is the most prominent form of head injury affecting the brain, with an estimated 1.7 million Americans affected each year (Kuhn 2012). Neurologists have been concerned about the danger of repeated head impacts since the 1920’s, but researchers have only begun to understand the long-term effects of rcTBI (McKee 2009). Although symptoms can be as mild as dizziness, current research suggests that multiple concussions can lead to a progressive degenerative brain disease known as chronic traumatic encephalopathy (CTE) (Luo 2008, McKee 2009, Kane 2013). Research on the brain is just beginning to ...
Adipose Tissue Therapeutics For Scar Rehabilitation After Thermal Injury, 2015 University of Massachusetts Medical School
Adipose Tissue Therapeutics For Scar Rehabilitation After Thermal Injury, Dylan Perry, Jorge R. Lujan-Hernandez, Ava Chappell, So Yun Min, Raghu Appasani, Raziel Rojas-Rodriguez, Michael S. Chin, Silvia Corvera, Janice F. Lalikos
Senior Scholars Program
Background: Burn injuries are common and in the long term can lead to hypertrophic or keloid scars, pain and pruritus, limited mobility across joints, and disfigurement. Numerous reports suggest adipose derived tissues, including adipose derived stem cells (ADSCs) and processed lipoaspirate, can improve acutely healing wounds from a variety of etiologies including excisional, thermal, and radiation injuries by both secretion of growth factors and direct differentiation. There are many options for scar treatment, including laser therapy, silicone sheets, steroid injection, and even skin grafting however these techniques either lack optimal efficacy or involve significant cost and morbidity. Clinical case series ...