Wnt Secretion Proteins Modulate Rankl-Induced Expression Of Aire In Thymic Epithelial Cells, 2018 CUNY City College
Wnt Secretion Proteins Modulate Rankl-Induced Expression Of Aire In Thymic Epithelial Cells, Daniel Pollack
Thymic epithelial cells (TEC) are essential for a proper adaptive immune response by regulating thymocyte development and establishing central tolerance. In the thymus, TECs differentially express Wnt proteins, which activate canonical and non-canonical Wnt signaling pathways. Wnt signaling is thought to regulate cell survival, proliferation, and development although the direct molecular mechanisms in TECs have yet to be elucidated. The inducible inhibition of canonical Wnt signaling with Dkk1 leads to a rapid loss of TEC progenitors as well as a decline in mature Aire-expressing mTECs. Therefore, we explore the role of Wnt ligands potentially responsible for stimulating and/or regulating ...
Dermatologist And Patient Preferences In Choosing Treatments For Moderate To Severe Psoriasis, 2017 University of Massachusetts Medical School
Dermatologist And Patient Preferences In Choosing Treatments For Moderate To Severe Psoriasis, Matthew Alcusky, Seina Lee, Gordon Lau, Gretchen R. Chiu, Nandini Hadker, Aparna Deshpande, Stephen Fleming, Nicola Vance, Steve Fakharzadeh
Open Access Articles
INTRODUCTION: The objective of the study was to determine the relative importance (RI) of treatment attributes psoriasis patients and physicians consider when choosing between biologic therapies based on psoriasis severity.
METHODS: A discrete choice experiment (DCE) weighting preference for eight sets of hypothetical treatments for moderate or severe psoriasis was conducted. DCE hypothetical treatments were defined and varied on combinations of efficacy, safety, and dosing attributes [frequency/setting/route of administration (ROA)].
RESULTS: When assuming moderate psoriasis in the patient DCE, ROA (RI 29%) and efficacy (RI 27%) drive treatment choices. When assuming severe disease in the DCE, patients preferred ...
Stromal Cell Subsets Directing Neonatal Spleen Regeneration, 2017 Bond University
Stromal Cell Subsets Directing Neonatal Spleen Regeneration, Jonathan K H Tan, Takeshi Watanabe
Faculty of Health Sciences & Medicine Publications
Development of lymphoid tissue is determined by interactions between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer's patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM-1(+)CD31(+)CD201(+) spleen stromal organizer cell-type ...
Namibia : Hiv As A National Issue, 2017 Augustana College, Rock Island Illinois
Namibia : Hiv As A National Issue, Elisabeth Vlasak
Global Public Health
Namibia is located on the southwestern coast of Africa, bordering Angola, Botswana, Zambia, South Africa, and the Atlantic Ocean. Namibia has a population of 2,265,000, as of 2016. Namibia has many challenges, including food insecurity and malnutrition, access to health services, unequal distribution of wealth, but HIV/AIDS is one of the country’s leading challenges. It is estimated that the overall adult infection rate of HIV/AIDS is 15.4% and it is the leading cause of death in Namibia. Adults ages 18-24 are the most at-risk for the disease. Some of the major risk factors are ...
Diverse Human Extracellular Rnas Are Widely Detected In Human Plasma, 2016 University of Massachusetts Medical School
Diverse Human Extracellular Rnas Are Widely Detected In Human Plasma, Jane E. Freedman, Eric Mick, Lea M. Beaulieu, Selim E. Tanriverdi, Milka Koupenova-Zamor, Ekaterina Mikhalev, Kahraman Tanriverdi
Open Access Articles
There is growing appreciation for the importance of non-protein-coding genes in development and disease. Although much is known about microRNAs, limitations in bioinformatic analyses of RNA sequencing have precluded broad assessment of other forms of small-RNAs in humans. By analysing sequencing data from plasma-derived RNA from 40 individuals, here we identified over a thousand human extracellular RNAs including microRNAs, piwi-interacting RNA (piRNA), and small nucleolar RNAs. Using a targeted quantitative PCR with reverse transcription approach in an additional 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence ...
Thymic Involution Perturbs Negative Selection And Leads To Chronic Inflammation, 2015 University of North Texas Health Science Center at Fort Worth
Thymic Involution Perturbs Negative Selection And Leads To Chronic Inflammation, Brandon D. Coder B.S.
Theses and Dissertations
The ubiquitous presence of chronic low-level pro-inflammatory factors in elderly individuals (termed inflammaging) is a significant risk factor for morbidity and mortality. The etiology of inflammaging is largely unknown. Recent evidence has identified the persistent activation of immune cells, thought to arise from latent viral infections, as key contributors towards the development of a chronic inflammatory environment. However, the contribution of autoreactive T cells towards the development of inflammaging has yet to be investigated. Another pervasive feature of the aging process is the age-related involution of the thymus gland, which has been linked with a predisposition toward developing autoimmunity. In ...
Specific Inflammatory Stimuli Lead To Distinct Platelet Responses In Mice And Humans, 2015 University of Massachusetts Medical School
Specific Inflammatory Stimuli Lead To Distinct Platelet Responses In Mice And Humans, Lea M. Beaulieu, Lauren Clancy, Kahraman Tanriverdi, Emelia J. Benjamin, Carolyn D. Kramer, Ellen O. Weinberg, Xianbao He, Samrawit Mekasha, Eric O. Mick, Robin R. Ingalls, Caroline A. Genco, Jane E. Freedman
Open Access Articles
INTRODUCTION: Diverse and multi-factorial processes contribute to the progression of cardiovascular disease. These processes affect cells involved in the development of this disease in varying ways, ultimately leading to atherothrombosis. The goal of our study was to compare the differential effects of specific stimuli - two bacterial infections and a Western diet - on platelet responses in ApoE-/- mice, specifically examining inflammatory function and gene expression. Results from murine studies were verified using platelets from participants of the Framingham Heart Study (FHS; n = 1819 participants).
METHODS: Blood and spleen samples were collected at weeks 1 and 9 from ApoE-/- mice infected with ...
Hemocyte Differentiation Mediates The Mosquito Late-Phase Immune Response Against Plasmodium In Anopheles Gambiae, 2015 Johns Hopkins Bloomberg School of Public Health
Hemocyte Differentiation Mediates The Mosquito Late-Phase Immune Response Against Plasmodium In Anopheles Gambiae, Ryan C. Smith, Carolina Barillas-Mury, Marcelo Jacobs-Lorena
Ryan C. Smith
Src Homology 2 Domain-Containing 5’-Inositol Phosphatase-2 (Ship2) Is An Effector Of Lymphatic Dysfunction, 2015 The University of Texas Graduate School of Biomedical Sciences at Houston
Src Homology 2 Domain-Containing 5’-Inositol Phosphatase-2 (Ship2) Is An Effector Of Lymphatic Dysfunction, Germaine D. Agollah
UT GSBS Dissertations and Theses (Open Access)
The lymphatic system is essential for the transport of excess fluid, protein, and foreign materials from interstitial tissues to lymph nodes; for immune surveillance, and to maintain fluid homeostasis. Dysregulated lymphatics can be attributed to pathological conditions including tumor metastasis, inflammation, chronic wounds, obesity, blood vascular disorders, and lymphedema. Of these, lymphedema is the most extreme of lymphatic disorders and is represented by a spectrum of symptoms ranging from mild, subtle presentation to severe, disfiguring, overt presentation. Lymphedema is more manageable in the early stages of disease but severely reduces quality of life with progression. Due to lack of molecular ...
Abcb5 Identifies Immunoregulatory Dermal Cells, 2015 Edith Cowan University
Abcb5 Identifies Immunoregulatory Dermal Cells, Tobias Schatton, Jun Yang, Sonja Kleffel, Mayuko Uehara, Steven R. Barthel, Christoph Schlapbach, Qian Zhan, Stephen Dudeney, Hansgeorg Mueller, Nayoung Lee, Juliane C. De Vries, Barbara Meier, Seppe Vander Beken, Mark M. Kluth, Christoph Ganss, Arlene H. Sharpe, Ana Maria Waaga-Gasser, Mohamed H. Sayegh, Reza Abdi, Karin Scharffetter-Kochanek, George F. Murphy, Thomas S. Kupper, Natasha Y. Frank, Markus H. Frank
ECU Publications Post 2013
Cell-based strategies represent a new frontier in the treatment of immune-mediated disorders. However, the paucity of markers for isolation of molecularly defined immunomodulatory cell populations poses a barrier to this field. Here, we show that ATP-binding cassette member B5 (ABCB5) identifies dermal immunoregulatory cells (DIRCs) capable of exerting therapeutic immunoregulatory functions through engagement of programmed cell death 1 (PD-1). Purified Abcb5+ DIRCs suppressed T cell proliferation, evaded immune rejection, homed to recipient immune tissues, and induced Tregs in vivo. In fully major-histocompatibility-complex-mismatched cardiac allotransplantation models, allogeneic DIRCs significantly prolonged allograft survival. Blockade of DIRC-expressed PD-1 reversed the inhibitory effects of ...
Evaluation Of The Contribution Of Multiple Damps And Damp Receptors In Cell Death-Induced Sterile Inflammatory Responses, 2014 University of Massachusetts Medical School
Evaluation Of The Contribution Of Multiple Damps And Damp Receptors In Cell Death-Induced Sterile Inflammatory Responses, Hiroshi Kataoka, Hajime Kono, Zubin Patel, Kenneth L. Rock
Open Access Articles
When cells die by necrosis in vivo they stimulate an inflammatory response. It is thought that this response is triggered when the injured cells expose proinflammatory molecules, collectively referred to as damage associated molecular patterns (DAMPs), which are recognized by cells or soluble molecules of the innate or adaptive immune system. Several putative DAMPs and/or their receptors have been identified, but whether and how much they participate in responses in vivo is incompletely understood, and they have not previously been compared side-by-side in the same models. This study focuses on evaluating the contribution of multiple mechanisms that have been ...
Pathscan Enabler At Md Anderson Cancer Center, 2014 M.D. Anderson Cancer Center
Pathscan Enabler At Md Anderson Cancer Center, George Mcnamara
McNamara 20140703 - Additional Pathscan and Tiki_Goddess related resources
Our Pathscan Enabler IV, delivered July 2014, uses the QuickScan software to scan a histology slide in "two clicks" (after loading the
1. Push the QuickScan button on the front of the Pathscan Enabler.
2. Click the Scan button in the QuickScan pop-up application (optional: change scan area in the Prescan image window).
The image gets saved as a TIFF file to ...
Nanotoxicity In Cells Of The Immune System, 2014 St. John Fisher College
Nanotoxicity In Cells Of The Immune System, Jonathan J. Pelc
Nanoparticles (NPs) provide a new medical approach to drug therapy. As with every new approach, safety precautions need to be taken, and the immediate and long-term effects for many NPs are still unclear. When administering a medical treatment into the human body, the first issue that needs to be addressed is host detection of the medicine and inflammation as a possible result of the treatment. If a new NP treatment causes inflammation before it releases its medicine, that treatment may be ineffective, even damaging to the patient. Small metallic and organic particles have been shown to elicit an inflammatory responses ...
Lymphatic Pump Treatment Enhances The Lymphatic And Immune System And Ameliorates Disease Severity In A Rat Model Of Respiratory Infection, 2014 University of North Texas Health Science Center
Lymphatic Pump Treatment Enhances The Lymphatic And Immune System And Ameliorates Disease Severity In A Rat Model Of Respiratory Infection, Artur Schander
Theses and Dissertations
The purpose of these studies was to explore the benefits, effects, and mechanisms of LPT in both a healthy and a diseased animal model, and hence provide scientific rationale for the clinical application of LPT.
Novel findings in this dissertation demonstrate that in anesthetized canines: 1) LPT mobilizes leukocytes from the GALT into lymphatic circulation; 2) LPT mobilizes inflammatory mediators into lymphatic circulation; and 3) repeated application of LPT increases lymph flow, concentration of leukocytes, and flux of inflammatory mediators into lymphatic circulation. In addition, this dissertation for the first time demonstrates: 1) the development of a novel lymph enhancing ...
Disposable Platform Provides Visual And Color-Based Point-Of-Care Anemia Self-Testing, 2014 Georgia Institute of Technology
Disposable Platform Provides Visual And Color-Based Point-Of-Care Anemia Self-Testing, Erika A. Tyburski, Scott E. Gillespie, William A. Stoy, Robert G. Mannino, Alexander J. Weiss, Alexa F. Siu, Rayford H. Bulloch, Karthik Thota, Anyela Cardenas, Wilena Session, Hanna J. Khoury, Siobhán O'Connor, Silvia T. Bunting, Jeanne Boudreaux, Craig R. Forest, Manila Gaddh, Traci Leong, L. Andrew Lyon, Wilbur A. Lam
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Anemia, or low blood hemoglobin (Hgb) levels, afflicts 2 billion people worldwide. Currently, Hgb levels are typically measured from blood samples using hematology analyzers, which are housed in hospitals, clinics, or commercial laboratories and require skilled technicians to operate. A reliable, inexpensive point-of-care (POC) Hgb test would enable cost-effective anemia screening and chronically anemic patients to self-monitor their disease. We present a rapid, standalone, and disposable POC anemia test that, via a single drop of blood, outputs color-based visual results that correlate with Hgb levels.
METHODS. We tested blood from 238 pediatric and adult patients with anemia of varying degrees ...
Cxcr7 Expression Disrupts Endothelial Cell Homeostasis And Causes Ligand-Dependent Invasion, 2014 Chapman University
Cxcr7 Expression Disrupts Endothelial Cell Homeostasis And Causes Ligand-Dependent Invasion, Jennifer Totonchy, Lisa Clepper, Kevin G. Phillips, Owen J. T. Mccarty, Ashlee V. Moses
Pharmacy Faculty Articles and Research
The homeostatic function of endothelial cells (EC ) is critical for a number of physiological processes including vascular integrity, immunity, and wound healing. Indeed, vascular abnormalities resulting from EC dysfunction contribute to the development and spread of malignancies. The alternative SDF-1/CXCL12 receptor CXCR7 is frequently and specifically highly expressed in tumor-associated vessels. In this study, we investigate whether CXCR7 contributes to vascular dysfunction by specifically examining the effect of CXCR7 expression on EC barrier function and motility. We demonstrate that CXCR7 expression in EC results in redistribution of CD31/PECAM-1 and loss of contact inhibition. Moreover, CXCR7+ EC are deficient ...
Aspects Of The Innate Immune System In The Caribbean Octocoral Swiftia Exserta, 2013 Florida International University
Aspects Of The Innate Immune System In The Caribbean Octocoral Swiftia Exserta, Lorenzo P. Menzel
FIU Electronic Theses and Dissertations
The immune systems of cnidaria are important to study for two reasons: to gain a better understanding of the evolution of immune responses, and to provide a basis to partially redress the precipitous world-wide die-offs of reef corals, some of which have been attributed to diseases and stress. Many immune responses share ancient evolutionary origins and are common across many taxa.
Using Swiftia exserta, an azooxanthellate ahermatypic local octocoral, as a proxy model organism to study aspects of innate immunity in corals and cnidaria allows us to address both of the reasons listed above while not using endangered species. Utilizing ...
Detection Of Immune Suppressive Neutrophils In Peripheral Blood Samples Of Cancer Patients, 2013 University of Massachusetts Medical School
Detection Of Immune Suppressive Neutrophils In Peripheral Blood Samples Of Cancer Patients, Suyang Hao, Mary Andersen, Hongbo Yu
University of Massachusetts Medical School Faculty Publications
Neutrophils provide first-line defense against infections and are potent effectors in innate and adaptive immunity. Recently neutrophils have been shown to play important roles in multiple antitumor reactions. A subset of mature neutrophils in human systemic inflammation has been identified as a unique circulating population of myeloid cells, which is capable of inhibiting T cell responses. These neutrophils show unique immunophenotype (CD11c bright/CD62L dim/CD11b bright/CD16 bright). This study reports detection of mature neutrophils with similar immunophenotype in the peripheral blood samples of cancer patients using flow cytometry analysis. This population of neutrophils is not detected in peripheral ...
The Effect Of Chronic Psychological Stress On The Cutaneous Immune Response In The Development Of A Contact Hypersensitivity Reaction, 2013 University of North Texas Health Science Center at Fort Worth
The Effect Of Chronic Psychological Stress On The Cutaneous Immune Response In The Development Of A Contact Hypersensitivity Reaction, Jessica M.F. Hall
Theses and Dissertations
Increasing evidence demonstrates that psychological stress can have an impact on the cutaneous immune response. While there have been many studies examining the impact of acute stress on an allergic contact dermatitis model, there are few studies concerning the impact of chronic psychological stress. Furthermore, the mechanism of the impact of chronic stress on contact hypersensitivity remains to be fully explored. Here we show that chronic restraint stress induces activation of the hypothalamus-pituitary-adrenal axis and delays weight gain in female BALB/c mice. In addition, we observed that chronic psychological stress reduces the cutaneous immune response in a contact hypersensitivity ...
Chikungunya Virus Infection Results In Higher And Persistent Viral Replication In Aged Rhesus Macaques Due To Defects In Anti-Viral Immunity, 2013 Oregon Health and Science University
Chikungunya Virus Infection Results In Higher And Persistent Viral Replication In Aged Rhesus Macaques Due To Defects In Anti-Viral Immunity, Ilhem Messaoudi, Jennifer Totonchy, Thomas Totonchy, Craig N. Kreklywich, Kristen Haberthur, Laura Springgay, James D. Brien, Michael S. Diamond, Victor R. Defilippis, Daniel N. Streblow
Pharmacy Faculty Articles and Research
Chikungunya virus (CHIKV) is a re-emerging mosquito-borne Alphavirus that causes a clinical disease involving fever, myalgia, nausea and rash. The distinguishing feature of CHIKV infection is the severe debilitating poly-arthralgia that may persist for several months after viral clearance. Since its re-emergence in 2004, CHIKV has spread from the Indian Ocean region to new locations including metropolitan Europe, Japan, and even the United States. The risk of importing CHIKV to new areas of the world is increasing due to high levels of viremia in infected individuals as well as the recent adaptation of the virus to the mosquito species Aedes ...