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Absence Of Typical Haversian System From The Compact Bone Of Some Reptile And Bird Species, Yasser Ahmed, Mohamed Abdelsabourv Khalaf, Fatma Khalil 2017 South Valley University

Absence Of Typical Haversian System From The Compact Bone Of Some Reptile And Bird Species, Yasser Ahmed, Mohamed Abdelsabourv Khalaf, Fatma Khalil

Histology

Background and Objective: Mammalian compact bone is composed mostly of Haversian system. Although there are many studies describing the typical Haversian system in mammals, there are few studies conducted on bones from non-mammalian species. The objective of the current study was to investigate the existence of the typical Haversian system in compact bones from reptiles and birds.

Materials and Methods: Femora were collected from geckos, Nile monitors, sparrows, ducks and geese. Samples were then, fixed in 10% paraformaldehyde and embedded in paraffin. Paraffin sections were stained with hematoxylin and eosin or Von Kossa and then examined using light microscopy.

Results ...


Taurine's Effect On Cocaine Reward And Neurogenesis In The Adolescent Male Rat Brain., Avery E. Villa-Gonzalez 2017 CUNY City College

Taurine's Effect On Cocaine Reward And Neurogenesis In The Adolescent Male Rat Brain., Avery E. Villa-Gonzalez

Master's Theses

Adolescence is a developmentally critical transition from childhood to adulthood including both maturation of the body and the brain. Neuroplastic changes result in dynamic organization of the brain during adolescence, leaving them vulnerable to development of mental illness and drug-seeking behavior. According to the United Nations Office on Drugs and Crime, the psychostimulant cocaine is the second most popular illicit drug in the world. Cocaine, amongst having many detrimental effects, has shown to also decrease hippocampal neurogenesis, resulting in decreased neuroplasticity and cognitive dysfunction. Previous students in our laboratory have shown that treatment of adult male rats with the essential ...


Localization And Distribution Of Primary Cilia In The Adult Mouse Heart, Ali Zarban, Hannah C. Saternos, Andrea L. Kalinoski, Lijun Liu, Surya M. Nauli, Wissam A. AbouAlaiwi 2016 University of Toledo

Localization And Distribution Of Primary Cilia In The Adult Mouse Heart, Ali Zarban, Hannah C. Saternos, Andrea L. Kalinoski, Lijun Liu, Surya M. Nauli, Wissam A. Aboualaiwi

Pharmacy Faculty Articles and Research

Although primary cilia have been shown to play crucial roles in the development of embryonic mouse heart, their presence and function in adult mouse heart remains controversial. In this study, the presence of primary cilia in adult mouse heart was investigated. The presence of primary cilia was initially demonstrated in the surface of cardiac cells of mouse hearts from both young and adult mice by immunostaining with acetylated α-tubulin, a ciliary structural marker. The presence of cardiac primary cilia in 1-, 3-, 6- and 12-month old mice was further confirmed by staining heart tissues with an antibody against pericentrin, a ...


Frozen Brain Atlas, Natalie Andrea Builes, Dominick Joseph Casciato, Samantha Taylor Bergoine, Robert Speth 2016 Nova Southeastern University

Frozen Brain Atlas, Natalie Andrea Builes, Dominick Joseph Casciato, Samantha Taylor Bergoine, Robert Speth

Frozen Brain Atlas

The Frozen Brain Atlas provides students, educators, and scientists with a tool to further explore the architecture of the brain. Current brain atlases allow users to navigate sectioned brain tissue using histological staining; however, they fail to capture naturally visible structures post sectioning. Through immediate post sectioning image collection, the natural contrast of the brain reveals unique structural details that are rarely seen by traditional staining procedures. By utilizing indexing and interactive labeling, the Nova Southeastern University Frozen Brain Atlas will allow identification of brain regions when used as a correlative tool for modalities including receptor autoradiography, immunofluorescence, and other ...


Morphological And Biochemical Adaptive Changes Associated With A Short-Period Starvation Of Adult Male Japanese Quail (Coturnix Japonica), Yasser Ahmed, Soha A. Soliman, Mohammed Abdelsabour-Khalaf 2016 South Valley University

Morphological And Biochemical Adaptive Changes Associated With A Short-Period Starvation Of Adult Male Japanese Quail (Coturnix Japonica), Yasser Ahmed, Soha A. Soliman, Mohammed Abdelsabour-Khalaf

Histology

Objective: The morphological and biochemical impact of a short-period of starvation on Japanese quail was investigated.

Materials and Methods: Ten adult male Japanese quail were divided into two groups; control fed and starved. The control-fed group was offered food and water ad libitum and the starved group was subjected to a short-period of food deprivation. After 2.5 days, the serum was obtained and different parameters including the total protein, AST, ALT, triglyceride, HDL, LDL, creatinine and urea were assessed. Gastrointestinal tract, stomach and liver were excised and their masses were estimated. Paraffin and resin embedded sections from the proventriculus ...


Fallow Bucks Get Hoarse: Vocal Fatigue As A Possible Signal To Conspecifics, Elisabetta Vannoni, Alan G. McElligott 2016 University of Zurich

Fallow Bucks Get Hoarse: Vocal Fatigue As A Possible Signal To Conspecifics, Elisabetta Vannoni, Alan G. Mcelligott

Alan G. McElligott, Ph.D.

Many studies of sexually selected vocal communication assume that calls remain stable throughout the breeding season. However, during this period, physiological and social factors change and these can have strong effects on the structure of calls and calling rates. During the rut, fallow bucks, Dama dama, reduce their feeding and increase the time and energy spent on vocalizing and fighting to gain matings, and consequently their body condition declines greatly. The availability of matings and intensity of competition between males also change. Therefore, we predicted that male vocal signalling would vary over time in response to the changing intersexual and ...


Acoustic Divergence In The Rut Vocalizations Of Persian And European Fallow Deer, J. B. Stachowicz, E. Vannoni, B. J. Pitcher, E. F. Briefer, E. Geffen, A. G. McElligott 2016 University of Zürich

Acoustic Divergence In The Rut Vocalizations Of Persian And European Fallow Deer, J. B. Stachowicz, E. Vannoni, B. J. Pitcher, E. F. Briefer, E. Geffen, A. G. Mcelligott

Alan G. McElligott, Ph.D.

We conducted a study of the male rut vocalizations (groans) of two closely related species, Persian and European fallow deer. Persian fallow deer are endangered, restricted to Iran and Israel, and their rut vocalizations have never been studied. By contrast, European fallow deer are one of the most common deer species in the world, and have been the subject of numerous detailed studies. Persian bucks are approximately 16% larger than European bucks, and this can have important implications for vocalizations. Persian bucks were recorded in Israel, and European bucks were recorded in the UK and Ireland. We measured temporal, fundamental ...


Food For Thought … Mechanistic Validation, Thomas Hartung, Sebastian Hoffman, Martin Stephens 2016 Johns Hopkins University

Food For Thought … Mechanistic Validation, Thomas Hartung, Sebastian Hoffman, Martin Stephens

Martin Stephens, Ph.D.

Validation of new approaches in regulatory toxicology is commonly defined as the independent assessment of the reproducibility and relevance (the scientific basis and predictive capacity) of a test for a particular purpose. In large ring trials, the emphasis to date has been mainly on reproducibility and predictive capacity (comparison to the traditional test) with less attention given to the scientific or mechanistic basis. Assessing predictive capacity is difficult for novel approaches (which are based on mechanism), such as pathways of toxicity or the complex networks within the organism (systems toxicology). This is highly relevant for implementing Toxicology for the 21st ...


Reduction Of Friction By Recombinant Human Proteoglycan 4 In Il-1Α Stimulated Bovine Cartilage Explants, Katherine M. Larson, Ling Zhang, Khaled A. Elsaid, Tannin A. Schmidt, Braden C. Fleming, Gary J. Badger, Gregory D. Jay 2016 Brown University

Reduction Of Friction By Recombinant Human Proteoglycan 4 In Il-1Α Stimulated Bovine Cartilage Explants, Katherine M. Larson, Ling Zhang, Khaled A. Elsaid, Tannin A. Schmidt, Braden C. Fleming, Gary J. Badger, Gregory D. Jay

Pharmacy Faculty Articles and Research

A boundary lubricant attaches and protects sliding bearing surfaces by preventing interlocking asperity-asperity contact. Proteoglycan-4 (PRG4) is a boundary lubricant found in the synovial fluid that provides chondroprotection to articular surfaces. Inflammation of the diarthrodial joint modulates local PRG4 concentration. Thus, we measured the effects of inflammation, with Interkeukin-1α (IL-1α) incubation, upon boundary lubrication and PRG4 expression in bovine cartilage explants. We further aimed to determine whether the addition of exogenous human recombinant PRG4 (rhPRG4) could mitigate the effects of inflammation on boundary lubrication and PRG4 expression in vitro.

Cartilage explants, following a 7-day incubation with IL-1α, were tested in ...


Genetic Analysis Reveals A Hierarchy Of Interactions Between Polycystin-Encoding Genes And Genes Controlling Cilia Function During Left-Right Determination, Daniel T. Grimes, Jennifer L. Keynton, Maria T. Buenavista, Xingjian Jin, Saloni H. Patel, Shinohara Kyosuke, Jennifer Vibert, Debbie J. Williams, Hiroshi Hamada, Rohana Hussain, Surya M. Nauli, Dominic P. Norris 2016 MRC Harwell

Genetic Analysis Reveals A Hierarchy Of Interactions Between Polycystin-Encoding Genes And Genes Controlling Cilia Function During Left-Right Determination, Daniel T. Grimes, Jennifer L. Keynton, Maria T. Buenavista, Xingjian Jin, Saloni H. Patel, Shinohara Kyosuke, Jennifer Vibert, Debbie J. Williams, Hiroshi Hamada, Rohana Hussain, Surya M. Nauli, Dominic P. Norris

Pharmacy Faculty Articles and Research

During mammalian development, left-right (L-R) asymmetry is established by a cilia-driven leftward fluid flow within a midline embryonic cavity called the node. This ‘nodal flow’ is detected by peripherally-located crown cells that each assemble a primary cilium which contain the putative Ca2+ channel PKD2. The interaction of flow and crown cell cilia promotes left side-specific expression of Nodal in the lateral plate mesoderm (LPM). Whilst the PKD2-interacting protein PKD1L1 has also been implicated in L-R patterning, the underlying mechanism by which flow is detected and the genetic relationship between Polycystin function and asymmetric gene expression remains unknown. Here, we ...


Rescuing Perishable Neuroanatomical Information From A Threatened Biodiversity Hotspot: Remote Field Methods For Brain Tissue Preservation Validated By Cytoarchitectonic Analysis, Immunohistochemistry, And X-Ray Microcomputed Tomography, Daniel F. Hughes, Ellen M. Walker, Paul M. Gignac, Anais Martinez, Kenichiro Negishi, Carl S. Lieb, Eli Greenbaum, Arshad M. Khan 2016 University of Texas at El Paso

Rescuing Perishable Neuroanatomical Information From A Threatened Biodiversity Hotspot: Remote Field Methods For Brain Tissue Preservation Validated By Cytoarchitectonic Analysis, Immunohistochemistry, And X-Ray Microcomputed Tomography, Daniel F. Hughes, Ellen M. Walker, Paul M. Gignac, Anais Martinez, Kenichiro Negishi, Carl S. Lieb, Eli Greenbaum, Arshad M. Khan

Arshad M. Khan, Ph.D.

Biodiversity hotspots, which harbor more endemic species than elsewhere on Earth, are
increasingly threatened. There is a need to accelerate collection efforts in these regions
before threatened or endangered species become extinct. The diverse geographical, ecological,
genetic, morphological, and behavioral data generated from the on-site collection of
an individual specimen are useful for many scientific purposes. However, traditional methods
for specimen preparation in the field do not permit researchers to retrieve neuroanatomical
data, disregarding potentially useful data for increasing our understanding of brain
diversity. These data have helped clarify brain evolution, deciphered relationships between
structure and function, and revealed constraints ...


Rescuing Perishable Neuroanatomical Information From A Threatened Biodiversity Hotspot: Remote Field Methods For Brain Tissue Preservation Validated By Cytoarchitectonic Analysis, Immunohistochemistry, And X-Ray Microcomputed Tomography, Daniel F. Hughes, Ellen M. Walker, Paul M. Gignac, Anais Martinez, Kenichiro Negishi, Carl S. Lieb, Eli Greenbaum, Arshad M. Khan 2016 University of Texas at El Paso

Rescuing Perishable Neuroanatomical Information From A Threatened Biodiversity Hotspot: Remote Field Methods For Brain Tissue Preservation Validated By Cytoarchitectonic Analysis, Immunohistochemistry, And X-Ray Microcomputed Tomography, Daniel F. Hughes, Ellen M. Walker, Paul M. Gignac, Anais Martinez, Kenichiro Negishi, Carl S. Lieb, Eli Greenbaum, Arshad M. Khan

Arshad M. Khan, Ph.D.

Biodiversity hotspots, which harbor more endemic species than elsewhere on Earth, are
increasingly threatened. There is a need to accelerate collection efforts in these regions
before threatened or endangered species become extinct. The diverse geographical, ecological,
genetic, morphological, and behavioral data generated from the on-site collection of
an individual specimen are useful for many scientific purposes. However, traditional methods
for specimen preparation in the field do not permit researchers to retrieve neuroanatomical
data, disregarding potentially useful data for increasing our understanding of brain
diversity. These data have helped clarify brain evolution, deciphered relationships between
structure and function, and revealed constraints ...


Localized Jnk Signaling Regulates Organ Size During Development., Helen Rankin Willsey, Xiaoyan Zheng, José Carlos Pastor-Pareja, A Jeremy Willsey, Philip A Beachy, Tian Xu 2016 George Washington University

Localized Jnk Signaling Regulates Organ Size During Development., Helen Rankin Willsey, Xiaoyan Zheng, José Carlos Pastor-Pareja, A Jeremy Willsey, Philip A Beachy, Tian Xu

Anatomy and Regenerative Biology Faculty Publications

A fundamental question of biology is what determines organ size. Despite demonstrations that factors within organs determine their sizes, intrinsic size control mechanisms remain elusive. Here we show that Drosophila wing size is regulated by JNK signaling during development. JNK is active in a stripe along the center of developing wings, and modulating JNK signaling within this stripe changes organ size. This JNK stripe influences proliferation in a non-canonical, Jun-independent manner by inhibiting the Hippo pathway. Localized JNK activity is established by Hedgehog signaling, where Ci elevates dTRAF1 expression. As the dTRAF1 homolog, TRAF4, is amplified in numerous cancers, these ...


Surface Scan Of The Parietal Fragment 1 Of Cpc 274, Brandon P. Hedrick 2016 University of Pennsylvania

Surface Scan Of The Parietal Fragment 1 Of Cpc 274, Brandon P. Hedrick

VP3D Scans

CPC 274, a new centrosaurine specimen from Mexico, was unearthed in northern Coahuila from 2007-2011. The majority of elements that were found with CPC 274 were surface scanned using a Polhemus FastSCAN system in order to allow readers the ability to manipulate the bones in a 3D environment to assess features directly. The files are .obj files and can be visualized in MeshLabTM, which can be downloaded for free (MeshLab, Visual Computing Lab – ISTI – CNR http://meshlab.sourceforge.net/).


Surface Scan Of The Squamosal Of Cpc 274, Brandon P. Hedrick 2016 University of Pennsylvania

Surface Scan Of The Squamosal Of Cpc 274, Brandon P. Hedrick

VP3D Scans

CPC 274, a new centrosaurine specimen from Mexico, was unearthed in northern Coahuila from 2007-2011. The majority of elements that were found with CPC 274 were surface scanned using a Polhemus FastSCAN system in order to allow readers the ability to manipulate the bones in a 3D environment to assess features directly. The files are .obj files and can be visualized in MeshLabTM, which can be downloaded for free (MeshLab, Visual Computing Lab – ISTI – CNR http://meshlab.sourceforge.net/).


Surface Scan Of The Premaxilla Of Cpc 274, Brandon P. Hedrick 2016 University of Pennsylvania

Surface Scan Of The Premaxilla Of Cpc 274, Brandon P. Hedrick

VP3D Scans

CPC 274, a new centrosaurine specimen from Mexico, was unearthed in northern Coahuila from 2007-2011. The majority of elements that were found with CPC 274 were surface scanned using a Polhemus FastSCAN system in order to allow readers the ability to manipulate the bones in a 3D environment to assess features directly. The files are .obj files and can be visualized in MeshLabTM, which can be downloaded for free (MeshLab, Visual Computing Lab – ISTI – CNR http://meshlab.sourceforge.net/).


Surface Scan Of The Parietal Fragment 2 Of Cpc 274, Brandon P. Hedrick 2016 University of Pennsylvania

Surface Scan Of The Parietal Fragment 2 Of Cpc 274, Brandon P. Hedrick

VP3D Scans

CPC 274, a new centrosaurine specimen from Mexico, was unearthed in northern Coahuila from 2007-2011. The majority of elements that were found with CPC 274 were surface scanned using a Polhemus FastSCAN system in order to allow readers the ability to manipulate the bones in a 3D environment to assess features directly. The files are .obj files and can be visualized in MeshLabTM, which can be downloaded for free (MeshLab, Visual Computing Lab – ISTI – CNR http://meshlab.sourceforge.net/).


Surface Scan Of The Dentary Of Cpc 274, Brandon P. Hedrick 2016 University of Pennsylvania

Surface Scan Of The Dentary Of Cpc 274, Brandon P. Hedrick

VP3D Scans

CPC 274, a new centrosaurine specimen from Mexico, was unearthed in northern Coahuila from 2007-2011. The majority of elements that were found with CPC 274 were surface scanned using a Polhemus FastSCAN system in order to allow readers the ability to manipulate the bones in a 3D environment to assess features directly. The files are .obj files and can be visualized in MeshLabTM, which can be downloaded for free (MeshLab, Visual Computing Lab – ISTI – CNR http://meshlab.sourceforge.net/).


Surface Scan Of The Scapula Of Cpc 274, Brandon P. Hedrick 2016 University of Pennsylvania

Surface Scan Of The Scapula Of Cpc 274, Brandon P. Hedrick

VP3D Scans

CPC 274, a new centrosaurine specimen from Mexico, was unearthed in northern Coahuila from 2007-2011. The majority of elements that were found with CPC 274 were surface scanned using a Polhemus FastSCAN system in order to allow readers the ability to manipulate the bones in a 3D environment to assess features directly. The files are .obj files and can be visualized in MeshLabTM, which can be downloaded for free (MeshLab, Visual Computing Lab – ISTI – CNR http://meshlab.sourceforge.net/).


Surface Scan Of The Femur Of Cpc 274, Brandon P. Hedrick 2016 University of Pennsylvania

Surface Scan Of The Femur Of Cpc 274, Brandon P. Hedrick

VP3D Scans

CPC 274, a new centrosaurine specimen from Mexico, was unearthed in northern Coahuila from 2007-2011. The majority of elements that were found with CPC 274 were surface scanned using a Polhemus FastSCAN system in order to allow readers the ability to manipulate the bones in a 3D environment to assess features directly. The files are .obj files and can be visualized in MeshLabTM, which can be downloaded for free (MeshLab, Visual Computing Lab – ISTI – CNR http://meshlab.sourceforge.net/).


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