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Long-Term Implications Of Neonatal Canine Caudectomies, Estefania Benavides 2020 Murray State University

Long-Term Implications Of Neonatal Canine Caudectomies, Estefania Benavides

Honors College Theses

Tail docking is an ongoing procedure several canine breeders choose to practice in order to preserve the expected appearance of certain breeds, as defined by the American Kennel Club (AKC). The spinal nerves are severed between the two caudal vertebrae of the neonate without the use of any anesthetics, local or general. The inability for the neonates to properly metabolize anesthetics contraindicates its administration. According to the American Veterinary Medical Association (AVMA), only two states in the United States have any sort of provision regarding canine caudectomies, and they are Maryland and Pennsylvania Generally in other states, experienced breeders and ...


Clinically Relevant 3d Characterization Of Simulated Non-Carious Cervical Dental Lesions And The Effects Of Toothbrush And Toothpaste Types On Wear, James Needy 2020 University of Arkansas, Fayetteville

Clinically Relevant 3d Characterization Of Simulated Non-Carious Cervical Dental Lesions And The Effects Of Toothbrush And Toothpaste Types On Wear, James Needy

Biomedical Engineering Undergraduate Honors Theses

This study sought to develop a novel method to quantitatively characterize non-carious cervical lesions (NCCLs) using an intraoral scanner (IOS) paired with Geomagic Wrap mesh software. The goal was to compare results with those obtained using an optical profilometer and Proscan superimposition software for clinical application. Comparisons were made using a previously published dataset aimed at measuring tooth loss associated with differing toothbrush and toothpaste types. Results show good comparability between methods and confirm that variation in brush stiffness and paste abrasivity result in significantly and predictably different levels of NCCL development. Extracted human maxillary premolars were assigned to one ...


Epidemiologic Survey Of Legionella Urine Antigen Testing Within A Large Wisconsin-Based Health Care System, Caroline P. Toberna, Hannah M. William, Jessica J. F. Kram, Kayla Heslin, Dennis J. Baumgardner 2020 Aurora Research Institute; Center for Urban Population Health; Aurora UW Medical Group, Aurora Health Care

Epidemiologic Survey Of Legionella Urine Antigen Testing Within A Large Wisconsin-Based Health Care System, Caroline P. Toberna, Hannah M. William, Jessica J. F. Kram, Kayla Heslin, Dennis J. Baumgardner

Journal of Patient-Centered Research and Reviews

Purpose: Legionella pneumophila pneumonia is a life-threatening, environmentally acquired infection identifiable via Legionella urine antigen tests (LUAT). We aimed to identify cumulative incidence, demographic distribution, and undetected disease outbreaks of Legionella pneumonia via positive LUAT in a single eastern Wisconsin health system, with a focus on urban Milwaukee County.

Methods: A multilevel descriptive ecologic study was conducted utilizing electronic medical record data from a large integrated health care system of patients who underwent LUAT from 2013 to 2017. A random sample inclusive of all positive tests was reviewed to investigate geodemographic differences among patients testing positive versus negative. Statistical comparisons ...


Ganglioside Detection From Formalin Fixed Human Brain Tissue Utilizing Maldi Imaging Mass Spectrometry, Aaron Harris 2020 The University of Western Ontario

Ganglioside Detection From Formalin Fixed Human Brain Tissue Utilizing Maldi Imaging Mass Spectrometry, Aaron Harris

Electronic Thesis and Dissertation Repository

Matrix assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) is largely performed on fresh frozen tissue whereas clinical tissue samples stored long term are fixed in formalin, and the fixation process is thought to cause signal suppression for lipid molecules. Studies show that fresh frozen brain tissue sections washed with ammonium formate (AF) solution prior to matrix application in the MALDI-IMS procedure display an increase in signal intensity and sensitivity for lipid molecules while maintaining molecular spatial distribution. Work in this thesis compares MALDI data of ganglioside molecules from fresh frozen and post-fixed rat brain samples, and post-fixed human ...


Content And Performance Of The Minimuga Genotyping Array, A New Tool To Improve Rigor And Reproducibility In Mouse Research, John Sebastian Sigmon, Christopher M. Sassetti, Martin T. Ferris, Leonard McMillan, Fernando Pardo-Manuel de Villena 2020 University of North Carolina

Content And Performance Of The Minimuga Genotyping Array, A New Tool To Improve Rigor And Reproducibility In Mouse Research, John Sebastian Sigmon, Christopher M. Sassetti, Martin T. Ferris, Leonard Mcmillan, Fernando Pardo-Manuel De Villena

University of Massachusetts Medical School Faculty Publications

The laboratory mouse is the most widely used animal model for biomedical research, due in part to its well annotated genome, wealth of genetic resources and the ability to precisely manipulate its genome. Despite the importance of genetics for mouse research, genetic quality control (QC) is not standardized, in part due to the lack of cost effective, informative and robust platforms. Genotyping arrays are standard tools for mouse research and remain an attractive alternative even in the era of high-throughput whole genome sequencing. Here we describe the content and performance of a new Mouse Universal Genotyping Array (MUGA). MiniMUGA, an ...


Detecting Chromatin Interactions Along And Between Sister Chromatids With Sisterc, Marlies E. Oomen, Adam K. Hedger, Jonathan K. Watts, Job Dekker 2020 University of Massachusetts Medical School

Detecting Chromatin Interactions Along And Between Sister Chromatids With Sisterc, Marlies E. Oomen, Adam K. Hedger, Jonathan K. Watts, Job Dekker

University of Massachusetts Medical School Faculty Publications

Accurate chromosome segregation requires chromosome compaction with concordant disentanglement of the two sister chromatids. This process has been studied extensively by microscopy but has remained a challenge for genomic methods, such as Hi-C, because sister chromatids have identical DNA sequences. Here we describe SisterC, a chromosome conformation capture assay that can distinguish interactions between and within sister chromatids. The assay is based on BrdU incorporation during S-phase, which labels the newly replicated strands of the sister chromatids. This is followed by Hi-C, e.g. during different stages of mitosis, and the selective destruction of BrdU containing strands by UV/Hoechst ...


Canine Models Of Inherited Musculoskeletal And Neurodegenerative Diseases, Brett D. Story, Matthew E. Miller, Allison M. Bradbury, Emily D. Million, Dongsheng Duan, Toloo Taghian, Dominik Faissler, Deborah Fernau, Sidney J. Beecy, Heather Gray-Edwards 2020 Auburn University

Canine Models Of Inherited Musculoskeletal And Neurodegenerative Diseases, Brett D. Story, Matthew E. Miller, Allison M. Bradbury, Emily D. Million, Dongsheng Duan, Toloo Taghian, Dominik Faissler, Deborah Fernau, Sidney J. Beecy, Heather Gray-Edwards

Open Access Articles

Mouse models of human disease remain the bread and butter of modern biology and therapeutic discovery. Nonetheless, more often than not mouse models do not reproduce the pathophysiology of the human conditions they are designed to mimic. Naturally occurring large animal models have predominantly been found in companion animals or livestock because of their emotional or economic value to modern society and, unlike mice, often recapitulate the human disease state. In particular, numerous models have been discovered in dogs and have a fundamental role in bridging proof of concept studies in mice to human clinical trials. The present article is ...


How The Wuhan Lockdown Significantly Cut Covid-19 Infections, Singapore Management University 2020 Singapore Management University

How The Wuhan Lockdown Significantly Cut Covid-19 Infections, Singapore Management University

Perspectives@SMU

CUHK study reveals the mandatory quarantine of the virus epicentre reduced infections by restricting mobility during the early stages of the outbreak and that social distancing policies in other cities were effective in containing the spread of the noval coronavirus


Simulation-Based Power And Sample Size Calculation For Designing Interrupted Time Series Analyses Of Count Outcomes In Evaluation Of Health Policy Interventions, Wei Liu, Shangyuan Ye, Bruce A. Barton, Melissa A. Fischer, Colleen Lawrence, Elizabeth J. Rahn, Maria I. Danila, Kenneth G. Saag, Paul A. Harris, Stephenie C. Lemon, Jeroan J. Allison, Bo Zhang 2020 Harbin Institute of Technology

Simulation-Based Power And Sample Size Calculation For Designing Interrupted Time Series Analyses Of Count Outcomes In Evaluation Of Health Policy Interventions, Wei Liu, Shangyuan Ye, Bruce A. Barton, Melissa A. Fischer, Colleen Lawrence, Elizabeth J. Rahn, Maria I. Danila, Kenneth G. Saag, Paul A. Harris, Stephenie C. Lemon, Jeroan J. Allison, Bo Zhang

Population and Quantitative Health Sciences Publications

Objective: The purpose of this study was to present the design, model, and data analysis of an interrupted time series (ITS) model applied to evaluate the impact of health policy, systems, or environmental interventions using count outcomes. Simulation methods were used to conduct power and sample size calculations for these studies.

Methods: We proposed the models and analyses of ITS designs for count outcomes using the Strengthening Translational Research in Diverse Enrollment (STRIDE) study as an example. The models we used were observation-driven models, which bundle a lagged term on the conditional mean of the outcome for a time series ...


Adaptive Evolution Targets A Pirna Precursor Transcription Network, Swapnil Parhad, Tianxiong Yu, Gen Zhang, Nicholas P. Rice, Zhiping Weng, William E. Theurkauf 2020 University of Massachusetts Medical School

Adaptive Evolution Targets A Pirna Precursor Transcription Network, Swapnil Parhad, Tianxiong Yu, Gen Zhang, Nicholas P. Rice, Zhiping Weng, William E. Theurkauf

Open Access Articles

In Drosophila, transposon-silencing piRNAs are derived from heterochromatic clusters and a subset of euchromatic transposon insertions, which are bound by the Rhino-Deadlock-Cutoff complex. The HP1 homolog Rhino binds to Deadlock, which recruits TRF2 to promote non-canonical transcription from both genomic strands. Cuff function is less well understood, but this Rai1 homolog shows hallmarks of adaptive evolution, which can remodel functional interactions within host defense systems. Supporting this hypothesis, Drosophila simulans Cutoff is a dominant-negative allele when expressed in Drosophila melanogaster, in which it traps Deadlock, TRF2, and the conserved transcriptional co-repressor CtBP in stable complexes. Cutoff functions with Rhino and ...


Population Modeling Of Tumor Growth Curves And The Reduced Gompertz Model Improve Prediction Of The Age Of Experimental Tumors, Cristina Vaghi, Anne Rodallec, Raphaelle Fanciullin, Joseph Ciccolini, Jonathan P. Mochel, Michalis Mastri, Clair Poignard, John M. L. Ebos, Sebastien Benzekry 2020 Inria Bordeaux Sud-Ouest

Population Modeling Of Tumor Growth Curves And The Reduced Gompertz Model Improve Prediction Of The Age Of Experimental Tumors, Cristina Vaghi, Anne Rodallec, Raphaelle Fanciullin, Joseph Ciccolini, Jonathan P. Mochel, Michalis Mastri, Clair Poignard, John M. L. Ebos, Sebastien Benzekry

Biomedical Sciences Publications

Tumor growth curves are classically modeled by means of ordinary differential equations. In analyzing the Gompertz model several studies have reported a striking correlation between the two parameters of the model, which could be used to reduce the dimensionality and improve predictive power. We analyzed tumor growth kinetics within the statistical framework of nonlinear mixed-effects (population approach). This allowed the simultaneous modeling of tumor dynamics and inter-animal variability. Experimental data comprised three animal models of breast and lung cancers, with 833 measurements in 94 animals. Candidate models of tumor growth included the exponential, logistic and Gompertz models. The exponential and ...


Budd-Chiari Syndrome From A Tunneled-Dialysis Catheter?, Ifrah Butt MD, Kairavee D. Dave MD, Lucas Rindy DO, Arash Zarrin DO, Livasky Concepcion MD, Daniel Heller MD, Franklin Kasmin MD, Steven Kaplan MD, John Rivas MD 2020 HCA Healthcare

Budd-Chiari Syndrome From A Tunneled-Dialysis Catheter?, Ifrah Butt Md, Kairavee D. Dave Md, Lucas Rindy Do, Arash Zarrin Do, Livasky Concepcion Md, Daniel Heller Md, Franklin Kasmin Md, Steven Kaplan Md, John Rivas Md

Internal Medicine

No abstract provided.


Seek And You Shall Find: Is Routine Duplex Ultrasound Screening For Deep Vein Thrombosis In Trauma Warranted?, Ashley Thompson MD, Krista Miller MD, Tatiana R. Eversley-Kelso MPH, Eric Shaw PhD, James Dunne MD, Heather MacNew MD, FACS 2020 HCA Healthcare

Seek And You Shall Find: Is Routine Duplex Ultrasound Screening For Deep Vein Thrombosis In Trauma Warranted?, Ashley Thompson Md, Krista Miller Md, Tatiana R. Eversley-Kelso Mph, Eric Shaw Phd, James Dunne Md, Heather Macnew Md, Facs

General Surgery

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Combining Citizen Science And Genomics To Investigate Tick, Pathogen, And Commensal Microbiome At Single-Tick Resolution, Gaurav Chauhan, Jesse McClure, Jessica Hekman, Patrick W. Marsh, Jeffrey A. Bailey, Rachel F. Daniels, Diane P. Genereux, Elinor K. Karlsson 2020 University of Massachusetts Medical School

Combining Citizen Science And Genomics To Investigate Tick, Pathogen, And Commensal Microbiome At Single-Tick Resolution, Gaurav Chauhan, Jesse Mcclure, Jessica Hekman, Patrick W. Marsh, Jeffrey A. Bailey, Rachel F. Daniels, Diane P. Genereux, Elinor K. Karlsson

Open Access Articles

The prevalence of tickborne diseases worldwide is increasing virtually unchecked due to the lack of effective control strategies. The transmission dynamics of tickborne pathogens are influenced by the tick microbiome, tick co-infection with other pathogens, and environmental features. Understanding this complex system could lead to new strategies for pathogen control, but will require large-scale, high-resolution data. Here, we introduce Project Acari, a citizen science-based project to assay, at single-tick resolution, species, pathogen infection status, microbiome profile, and environmental conditions of tens of thousands of ticks collected from numerous sites across the United States. In the first phase of the project ...


Prediction Of Molecular Mutations In Diffuse Low-Grade Gliomas Using Mr Imaging Features, Zeina A. Shboul, James Chen, Khan M. Iftekharrudin 2020 Old Dominion University

Prediction Of Molecular Mutations In Diffuse Low-Grade Gliomas Using Mr Imaging Features, Zeina A. Shboul, James Chen, Khan M. Iftekharrudin

Electrical & Computer Engineering Faculty Publications

Diffuse low-grade gliomas (LGG) have been reclassified based on molecular mutations, which require invasive tumor tissue sampling. Tissue sampling by biopsy may be limited by sampling error, whereas non-invasive imaging can evaluate the entirety of a tumor. This study presents a non-invasive analysis of low-grade gliomas using imaging features based on the updated classification. We introduce molecular (MGMT methylation, IDH mutation, 1p/19q co-deletion, ATRX mutation, and TERT mutations) prediction methods of low-grade gliomas with imaging. Imaging features are extracted from magnetic resonance imaging data and include texture features, fractal and multi-resolution fractal texture features, and volumetric features. Training models ...


Spectrographic Analysis Of The Lombard Effect, Julia Saxon 2020 The University of Akron

Spectrographic Analysis Of The Lombard Effect, Julia Saxon

Williams Honors College, Honors Research Projects

In my honors project I will study the feasibility of using spectrographic analysis to measure the Lombard effect. The Lombard effect is a well-known phenomenon in which talkers reflexively increase the intensity of their voices in the presence of background noise. We hypothesize that other measurable features of speech will also change with vocal intensity consistent with what is shown in literature. However, the current authors will discuss findings in terms of its potential value as a test of pseudohypoacusis, also known as nonorganic or functional hearing loss. The author and a licensed audiologist will examine the /i/ and /u ...


Tick-Borne Infections In New Hampshire: An Evaluation Of The Diagnostic Process In A Local Patient Population, Katherine Anderson 2020 University of New Hampshire, Durham

Tick-Borne Infections In New Hampshire: An Evaluation Of The Diagnostic Process In A Local Patient Population, Katherine Anderson

Honors Theses and Capstones

Overall, approximately 95 percent of reported cases of vector-borne disease were associated with ticks, making these the most medically important group of arthropods in the United States.1 Despite the prevalence of tick-borne infections, the process for the diagnosis of this condition is not well studied. This study aims to analyze data from a pool of 100 patients who underwent testing for tick-borne disease in the same institution in Dover, New Hampshire during the most recent peak tick season of 2019. Information utilized in this study included: patient age, sex, location of testing (inpatient versus outpatient), diagnostic testing methods used ...


Evaluating The Occurrence Of Age-Related Peripheral Neuropathy In Het3 Mice And Development Of A Whole Tissue Imaging Technique For Analyzing Total Innervation In The Subcutaneous Adipose Depot, Jake Willows 2019 University of Maine

Evaluating The Occurrence Of Age-Related Peripheral Neuropathy In Het3 Mice And Development Of A Whole Tissue Imaging Technique For Analyzing Total Innervation In The Subcutaneous Adipose Depot, Jake Willows

Electronic Theses and Dissertations

Adipose tissue can be characterized as either being a white (energy storing) depot or a brown (energy expending) depot and both have been found to contain dense networks of neural innervation. This adipose nerve supply regulates numerous metabolic functions and likely plays an important role in the function of adipose blood vessels. Recently our lab has shown in the C57BL/6 mouse model that peripheral neuropathy, or the dying back and dysfunction of the nerves in the superficial tissues such as the skin, can extend into the subcutaneous adipose tissue in conditions commonly associated with the neuropathic phenotype (i.e ...


Safety Of Endoscopic Retrograde Cholangiopancreatography (Ercp) In Pregnancy: A Systematic Review And Meta-Analysis, Mohamed Azab, Shishira Bharadwaj, Mahendran Jayaraj, Annie S. Hong, Pejman Solaimani, Mohamad Mubder, Hyeyoung Yeom, Ji Won Yoo, Michael L. Volk 2019 Loma Linda University Medical Center

Safety Of Endoscopic Retrograde Cholangiopancreatography (Ercp) In Pregnancy: A Systematic Review And Meta-Analysis, Mohamed Azab, Shishira Bharadwaj, Mahendran Jayaraj, Annie S. Hong, Pejman Solaimani, Mohamad Mubder, Hyeyoung Yeom, Ji Won Yoo, Michael L. Volk

School of Medicine Faculty Publications

Background/Aims: Endoscopic retrograde cholangiopancreatography (ERCP) is a technically challenging procedure rarely associated with severe postprocedure complications. Hormonal changes during pregnancy promote cholelithiasis, but there are limited clinical data available on the outcomes of ERCP in pregnant women. ERCP techniques without irradiation were recently introduced as potential alternative. We performed a systematic review and meta-analysis to assess the safety of ERCP in pregnancy and to compare outcomes of radiation versus nonradiation ERCP. Materials and Methods: A systematic search of PubMed, Medline/Ovid, Web of Science, and Google Scholar through April 18th, 2018 using PRISMA and MOOSE guidelines identified 27 studies ...


Evolution Of Acute Ischemic Stroke Treatment As Supported By Clinical Trials, Savya S. Shukla, Brian F. Baigorri MD, Roberto I. Fourzali MD, Yafell Serulle MD 2019 HCA Healthcare

Evolution Of Acute Ischemic Stroke Treatment As Supported By Clinical Trials, Savya S. Shukla, Brian F. Baigorri Md, Roberto I. Fourzali Md, Yafell Serulle Md

Radiology

No abstract provided.


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