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Anthelminthic Screening For Parasitic Nematodes, Elfawal A. Mostafa, Dan Lawler, Dirk Albrecht, Raffi V. Aroian 2016 University of Massachusetts Medical School

Anthelminthic Screening For Parasitic Nematodes, Elfawal A. Mostafa, Dan Lawler, Dirk Albrecht, Raffi V. Aroian

UMass Center for Clinical and Translational Science Research Retreat

For many parasitic diseases, high-throughput phenotypic screening is an important tool in finding new drugs. Some of the most important parasitic diseases are caused by nematodes. However, these parasitic nematodes are not typically amenable to high throughput screening. Due to the ease of its maintenance and suitability for high throughput assay, the nematode Caenorhabditis elegans is instead used. To address whether C. elegans is a good model for nematode drug discovery, we compared the drug susceptibility of C. elegans relative to the human hookworm nematode parasite Ancylostoma ceylanicum at several developmental stages using a library of FDA approved drugs. I ...


Text Mining From Drug Surveillance Report Narratives, Susmitha Wunnava, Tabassum Kakar, Xiao Qi, Elke A. Rudensteiner 2016 Worcester Polytechnic Institute

Text Mining From Drug Surveillance Report Narratives, Susmitha Wunnava, Tabassum Kakar, Xiao Qi, Elke A. Rudensteiner

UMass Center for Clinical and Translational Science Research Retreat

Analysis of postmarket drug surveillance reports is imperative to ensure drug safety and effectiveness. FAERS (FDA Adverse Event Reporting System) is a surveillance system that monitors Adverse Events (AEs) from drugs and biologic products. The AEs are reported through MedWatch voluntary reports (initiated from patients and healthcare providers) and mandatory reports (initiated from manufacturers). Much of the information in the voluntary AE reports is narratives or unstructured text. The increasing volume of individual reports, estimated at more than one million per year, poses a challenge for the staff to review large volume of narratives for drug clinical review. We are ...


Invitrometrix Qcm-Based Cell Biosensor: Research Tool To Accelerate Pharmaceutical Drug Discovery Success, Abiche H. Dewilde 2016 University of Massachusetts Lowell

Invitrometrix Qcm-Based Cell Biosensor: Research Tool To Accelerate Pharmaceutical Drug Discovery Success, Abiche H. Dewilde

UMass Center for Clinical and Translational Science Research Retreat

As part of the mini-symposium entitled "Shark Tank-UMass Spin-out Life Sciences Start-ups," Dr. Dewilde discusses an example of UMass life sciences technology that has been spun out into a start-up company -- Cell QCM from UMass Lowell.


Detecting Heart Failure Decompensation By Measuring Transthoracic Bioimpedance In The Outpatient Setting: Rationale And Design Of The Sentinel-Hf Study, Silviu Dovancescu, Jane Saczynski, Chad Darling, Jarno Riistema, Fatima Kuniyoshi, Theo Meyer, Robert Goldberg, David McManus 2016 Philips Research

Detecting Heart Failure Decompensation By Measuring Transthoracic Bioimpedance In The Outpatient Setting: Rationale And Design Of The Sentinel-Hf Study, Silviu Dovancescu, Jane Saczynski, Chad Darling, Jarno Riistema, Fatima Kuniyoshi, Theo Meyer, Robert Goldberg, David Mcmanus

David D. McManus

BACKGROUND: Recurrent hospital admissions are common among patients admitted for acute decompensated heart failure (ADHF), but identification of patients at risk for rehospitalization remains challenging. Contemporary heart failure (HF) management programs have shown modest ability to reduce readmissions, partly because they monitor signs or symptoms of HF worsening that appear late during decompensation. Detecting early stages of HF decompensation might allow for immediate application of effective HF therapies, thereby potentially reducing HF readmissions. One of the earliest indicators of HF decompensation is intrathoracic fluid accumulation, which can be assessed using transthoracic bioimpedance.

OBJECTIVE: The SENTINEL-HF study is a prospective observational ...


Induced Hypothermia Post Cardiac Resuscitation Works In Hospitalized Patients But Not During Transport To Hospital, A Logical Inconsistency Of Evidence, Joshua Frederick 2016 Wayne State University

Induced Hypothermia Post Cardiac Resuscitation Works In Hospitalized Patients But Not During Transport To Hospital, A Logical Inconsistency Of Evidence, Joshua Frederick

Clinical Research in Practice: The Journal of Team Hippocrates

A critical appraisal and clinical application of Kim F, Nichol G, Maynard C, et al. Effect of prehospital induction of mild hypothermia on survival and neurological status among adults with cardiac arrest. JAMA 2013;311(1):45-52. doi:10.1001/jama.2013.282173


Biventricular Finite Element Modeling And Quantification Of 3d Langragian Strains And Torsion Using Dense Mri, Zhanqiu Liu 2016 University of Kentucky

Biventricular Finite Element Modeling And Quantification Of 3d Langragian Strains And Torsion Using Dense Mri, Zhanqiu Liu

Theses and Dissertations--Mechanical Engineering

Statistical data suggests that increased use of evidence-based medical therapies has largely contributed to the decrease in American death rate caused by heart disease. And my studies are about two applications of magnetic resonance imaging (MRI) as a non-invasive approach in evidence-based health care research. In my first study, the achievement of a pulmonary valve replacement surgery was assessed on a patient with tetralogy of Fallot (TOF). In order to evaluate the remodeling of right ventricle, two biventricular finite element models were built up for pre-surgical images and post-surgical images. In my second study, 3D Lagrangian strains and torsion in ...


Sex Differences In The Efficacy Of Combined Il-10 And Il-10r1 Gene Therapy For Neuropathic Pain In Mice, J Logan Brock 2016 University of Colorado, Boulder

Sex Differences In The Efficacy Of Combined Il-10 And Il-10r1 Gene Therapy For Neuropathic Pain In Mice, J Logan Brock

Undergraduate Honors Theses

Neuropathic pain is a debilitating condition that affects millions of Americans. Despite decades of research, the disease is refractory to current treatments in a substantial population of patients. Recent advances in the understanding of neuropathic pain have led to the targeting of glial cells to better control the condition. Current clinical therapies do not manage the inflammatory response that originates with glial cells and contributes to this affliction. IL-10 is an anti-inflammatory cytokine that attenuates the inflammatory response that occurs at the neuroimmune interface in neuropathic pain models. IL-10 gene therapy has alleviated neuropathic pain in a number of animal ...


Arsenic Dysbiosis To The Zebrafish Microbiome: An Ecological Or Physiological Response?, Jonathan Michael Von Reusner 2016 Bard College

Arsenic Dysbiosis To The Zebrafish Microbiome: An Ecological Or Physiological Response?, Jonathan Michael Von Reusner

Senior Projects Spring 2016

In this study, we sought to demonstrate how the dysbiosis in zebrafish (D.rerio) that’s observed with low levels of arsenic (50ppb) could be a physiological response, rather than a purely ecological consequence of arsenic’s direct toxicity to microbial symbionts. To probe this question, we applied 16S based metagenomics to observe arsenic’s effects on the fish media, where no physiological processes exist, as well as how immunosuppressive doses of dexamethasone treatment (50ug/ml) may reflect arsenic dysbiosis within the fish microbiome. Consistent with arsenic’s cytotoxic potential, arsenic reduced average number of 16S sequences across both fish ...


Adhd And Social Impairment Among Children: Problem Solving Skills As A Potential Mediator, Rebecca McCord 2015 University of South Carolina - Columbia

Adhd And Social Impairment Among Children: Problem Solving Skills As A Potential Mediator, Rebecca Mccord

Senior Theses

This study tested the hypothesis that problem solving skills mediate the relation between ADHD symptoms and social impairment in children. Problem solving skills were evaluated by the Test of Problem Solving 3 – Elementary Version (TOPS), which separated problem solving skills into six different subtypes: making inferences, sequencing, negative questions, problem solving, predicting, and determining causes. ADHD symptoms were assessed through the parent report of the Disruptive Behavior Disorder Scale (DBD). Social impairment was assessed through the parent report of social skills using the Social Skills Improvement System (SSIS). Three hundred seventy-two (372) children between the ages of eight and ten ...


The Mind-Body Connection: The Association Between Adolescent Locus Of Control And Indicators Of Physical Health, C. Brahler, James Cropper 2015 University of Dayton

The Mind-Body Connection: The Association Between Adolescent Locus Of Control And Indicators Of Physical Health, C. Brahler, James Cropper

C. Jayne Brahler

Locus of control (LOC) describes an individual’s generalized beliefs or expectancies that their reinforcements are under internal versus external control (1). An individual exhibits either an internal or external LOC. This study examines the link between LOC and selected health risk factors in adolescents. A convenience sample of 167 high school physical education students completed a 13-item LOC questionnaire based on Rotter’s 1966 instrument. Various anthropometric measurements, blood pressure, body mass index (BMI), and body fat were recorded on all subjects. A subsample of 61 female students received blood chemistry analysis that included a lipid profile, hemoglobin A1c ...


Interaction Of Alu Polymorphisms And Novel Measures Of Discrimination In Association With Blood Pressure In African Americans Living In Tallahassee, Alexis C. Boulter, Jackyn Quinlan, Aida T. Miró-Herrans, Laurel N. Pearson, Nubiana L. Todd, Clarence C. Gravlee, Connie J. Mulligan 2015 University of Florida

Interaction Of Alu Polymorphisms And Novel Measures Of Discrimination In Association With Blood Pressure In African Americans Living In Tallahassee, Alexis C. Boulter, Jackyn Quinlan, Aida T. Miró-Herrans, Laurel N. Pearson, Nubiana L. Todd, Clarence C. Gravlee, Connie J. Mulligan

Human Biology Open Access Pre-Prints

African Americans are 40% more likely to be afflicted with hypertension in comparison to non-Hispanic, white Americans, resulting in a 30% higher instance of mortality due to cardiovascular disease. There is debate about the relative contributions of genetic and sociocultural risk factors to the racial disparity in hypertension.

We assayed three Alu insertion polymorphisms located in the angiotensin-1-converting enzyme (ACE), tissue plasminogen activator (PLAT), and with no-lysine kinase 1 (WNK1) genes. We also estimated West African genetic ancestry and developed novel measures of perceived discrimination to create a biocultural model of blood pressure among African- American adults in Tallahassee, FL ...


A Response-Adaptive Covariate-Balanced Randomization For Multi-Arm Clinical Trials, Cassandra M. Ballou 2015 Oregon Health & Science University

A Response-Adaptive Covariate-Balanced Randomization For Multi-Arm Clinical Trials, Cassandra M. Ballou

Scholar Archive

Randomization is a key characteristic of clinical trials which makes them the gold standard for determining treatment effectiveness. Response-adaptive randomization is desirable because it allows more patients to receive the best treatment; however, compared to traditional equal randomization, response-adaptive randomization is more likely to allow imbalance in prognostic baseline covariates. We propose a simple yet flexible two-stage randomization for multi-arm trials which marries response-adaptative and covariate-balancing designs. The operating characteristics of the proposed methods were assessed via simulation for a variety of scenarios in which values of treatment success probability and patient response delay time were varied. The newly proposed ...


Reliability Of Bod Pod Measurements Remain High Following A Short Duration Low-Carbohydrate Diet, Beau Greer, Kathleen Edsall (Alumna), Anna Greer 2015 Sacred Heart University

Reliability Of Bod Pod Measurements Remain High Following A Short Duration Low-Carbohydrate Diet, Beau Greer, Kathleen Edsall (Alumna), Anna Greer

Anna E. Greer

The purpose of the present study was to determine whether expected changes in body weight via a three day low-carbohydrate (CHO) diet will disrupt the reliability of air displacement plethysmography measurements via BOD POD. Twenty-four subjects recorded their typical diets for three days prior to BOD POD and seven-site skinfold analyses. Subjects were matched for lean body mass and divided into low-CHO (LC) and control (CON) groups. The LC group was given instruction intended to prevent over 50 grams/day of carbohydrate consumption for three consecutive days, while the CON group replicated their previously recorded diet. Body composition measurements were ...


Detecting Heart Failure Decompensation By Measuring Transthoracic Bioimpedance In The Outpatient Setting: Rationale And Design Of The Sentinel-Hf Study, Silviu Dovancescu, Jane S. Saczynski, Chad E. Darling, Jarno Riistema, Fatima Sert Kuniyoshi, Theo E. Meyer, Robert J. Goldberg, David D. McManus 2015 Philips Research

Detecting Heart Failure Decompensation By Measuring Transthoracic Bioimpedance In The Outpatient Setting: Rationale And Design Of The Sentinel-Hf Study, Silviu Dovancescu, Jane S. Saczynski, Chad E. Darling, Jarno Riistema, Fatima Sert Kuniyoshi, Theo E. Meyer, Robert J. Goldberg, David D. Mcmanus

University of Massachusetts Medical School Faculty Publications

BACKGROUND: Recurrent hospital admissions are common among patients admitted for acute decompensated heart failure (ADHF), but identification of patients at risk for rehospitalization remains challenging. Contemporary heart failure (HF) management programs have shown modest ability to reduce readmissions, partly because they monitor signs or symptoms of HF worsening that appear late during decompensation. Detecting early stages of HF decompensation might allow for immediate application of effective HF therapies, thereby potentially reducing HF readmissions. One of the earliest indicators of HF decompensation is intrathoracic fluid accumulation, which can be assessed using transthoracic bioimpedance.

OBJECTIVE: The SENTINEL-HF study is a prospective observational ...


T2 Relaxation Time In Men And Women Following Eccentric Elbow Flexor Exercise, Patrick A. Moull 2015 The University of Western Ontario

T2 Relaxation Time In Men And Women Following Eccentric Elbow Flexor Exercise, Patrick A. Moull

Electronic Thesis and Dissertation Repository

Previous research suggests that estrogen exhibits a protective effect on skeletal muscle and may help curb the effects of induced damage. Eccentric (ECC) exercise has been shown to provide the greatest levels of exercise induced muscle damage (EIMD) and with indirect markers of muscle damage including decreases in strength, increased inflammation, and higher magnitudes of delayed onset muscle soreness (DOMS). Studies have shown that elevations of the T2 relaxation times of activated muscles coincide with these other indirect measures of muscle damage. The purpose of this study was to compare the effects of a high intensity ECC elbow flexor exercise ...


Reliability Of Bod Pod Measurements Remain High Following A Short Duration Low-Carbohydrate Diet, Beau K. Greer, Kathleen M. Edsall (Alumna), Anna E. Greer 2015 Sacred Heart University

Reliability Of Bod Pod Measurements Remain High Following A Short Duration Low-Carbohydrate Diet, Beau K. Greer, Kathleen M. Edsall (Alumna), Anna E. Greer

PTHMS Faculty Publications

The purpose of the present study was to determine whether expected changes in body weight via a three day low-carbohydrate (CHO) diet will disrupt the reliability of air displacement plethysmography measurements via BOD POD. Twenty-four subjects recorded their typical diets for three days prior to BOD POD and seven-site skinfold analyses. Subjects were matched for lean body mass and divided into low-CHO (LC) and control (CON) groups. The LC group was given instruction intended to prevent over 50 grams/day of carbohydrate consumption for three consecutive days, while the CON group replicated their previously recorded diet. Body composition measurements were ...


Fast Indication Of Bacterial Growth In Clinical Specimens By The Pmeu Approach, Elias Hakalehto, Anneli Heitto, Ilkka Pesola, Eva Del Amo, Heikki Paakkanen, Osmo Hänninen, Jouni Pesola 2015 University of Windsor

Fast Indication Of Bacterial Growth In Clinical Specimens By The Pmeu Approach, Elias Hakalehto, Anneli Heitto, Ilkka Pesola, Eva Del Amo, Heikki Paakkanen, Osmo Hänninen, Jouni Pesola

21st International Conference on Environmental Indicators (ICEI 2015)

No abstract provided.


Development Of A Comprehensive Massively Parallel Sequencing Panel Of Single Nucleotide Polymorphism And Short Tandem Repeat Markers For Human Identification, David H. Warshauer 2015 University of North Texas Health Science Center at Fort Worth

Development Of A Comprehensive Massively Parallel Sequencing Panel Of Single Nucleotide Polymorphism And Short Tandem Repeat Markers For Human Identification, David H. Warshauer

Theses and Dissertations

Massively parallel sequencing (MPS) technologies allow for the detection of an unparalleled amount of genetic information with unprecedented speed and relative ease. These qualities make the technology desirable for generating DNA profiles that may be uploaded into forensic offender, arrestee, and family reference database files. This doctoral dissertation research was conducted under the hypothesis that MPS, with its exquisitely high throughput, can provide a system whereby reference samples can be typed for a large battery of markers, providing more discrimination power for forensic DNA typing and offering increased opportunities to develop investigative leads. The design and implementation of large marker ...


Functional Analysis Of The Molluscum Contagiosum Virus Mc160 Death Effector Domain-Containing Protein Rxdl Motif, Sarah Weber 2015 Seton Hall University

Functional Analysis Of The Molluscum Contagiosum Virus Mc160 Death Effector Domain-Containing Protein Rxdl Motif, Sarah Weber

Seton Hall University Dissertations and Theses (ETDs)

The Molluscum contagiosum virus (MCV) is a member of the Poxviridae family that causes benign skin lesions. MCV lesions persist on average for 8-12 months in otherwise healthy individuals. MCV lesions are characterized by reduced inflammation. The persistence and reduction of inflammation at the site of MCV lesions have been attributed to MCV immune evasion genes. MCV encodes two death effector domain (DED) containing proteins, MC159 and MC160. DEDs are found in cellular proteins such as FADD and procaspase-8. These cellular proteins are involved in several innate immune responses such as apoptosis and activation of interferon (IFN). Presumably, MC159 and ...


Cell Line Authentication And Contamination Assessment For Human Cell Cultures, Andrea Ormos B.S. 2015 University of North Texas Health Science Center at Fort Worth

Cell Line Authentication And Contamination Assessment For Human Cell Cultures, Andrea Ormos B.S.

Theses and Dissertations

Cell line authentication is an essential step in ensuring the integrity and reproducibility of biomedical research. The major contaminants in cell cultures are fungi, viruses, bacteria and contamination from other cell lines of the same or different species. Contaminants alter the physiology and properties of cells, compromising the results of experiments. In this study, an improved multiplex assay was developed, detecting mycoplasma and mouse cell line contamination, while performing DNA typing.

The assay was tested on cell cultures, the reproducibility of the assay was verified, sample collection and procedures were optimized and limit of detection for contaminants were determined. A ...


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