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Genetic Variation In The Perennial Ryegrass Fungal Endophyte Neotyphodium Lolii, E. van Zijll de Jong, N. R. Bannan, A. V. Stewart, K. F. M. Reed, M. P. Dobrowolski, K. F. Smith, G. C. Spangenberg, J. W. Forster 2023 La Trobe University, Australia

Genetic Variation In The Perennial Ryegrass Fungal Endophyte Neotyphodium Lolii, E. Van Zijll De Jong, N. R. Bannan, A. V. Stewart, K. F. M. Reed, M. P. Dobrowolski, K. F. Smith, G. C. Spangenberg, J. W. Forster

International Grassland Congress Proceedings

The common fungal endophytes (Neotyphodium species) of temperate pasture grasses are associated with improved tolerance to water and nutrient stress and resistance to insect pests, but are also the causal agents of animal toxicoses. Considerable variation exists among grass-endophyte associations for these beneficial and detrimental agronomic traits. The extent to which this variation may be attributed to the endophyte genotype, the host genotype or environmental interactions is currently unknown. The development of molecular genetic markers for endophytes based on simple sequence repeat (SSR) loci and the demonstration of the specific detection of endophytes in planta with these markers (van …


Metabolome Analysis Of The Interaction Between Perennial Ryegrass (Lolium Perenne) And The Fungal Endophyte Neotyphodium Lolii, P. Tian, S. Felitti, M. P. Dobrowolski, K. F. Smith, D. Edwards, R. Hall, J. Kopka, G. C. Spangenberg 2023 Molecular Plant Breeding Cooperative Research Centre, Australia

Metabolome Analysis Of The Interaction Between Perennial Ryegrass (Lolium Perenne) And The Fungal Endophyte Neotyphodium Lolii, P. Tian, S. Felitti, M. P. Dobrowolski, K. F. Smith, D. Edwards, R. Hall, J. Kopka, G. C. Spangenberg

International Grassland Congress Proceedings

Perennial ryegrass (Lolium perenne L.) and tall fescue (Festuca arundinacea Schreb.) frequently contain endophytic fungi (Neotyphodium lolii in perennial ryegrass and N. coenophialum in tall fescue). The presence of the endophyte has been shown to improve seedling vigour, persistence and drought tolerance in marginal environments as well as provide protection against some insect pests. Endophyte-infected grasses also produce a wide range of metabolites, including ergopeptine alkaloids, indole-isoprenoid lolitrems, pyrrolizidine alkaloids, and pyrrolopyrazine alkaloids. In contrast to information on alkaloids and animal toxicosis, the beneficial physiological aspects of the endophyte/grass interactions have not been well characterised. The physiological …


Endophyte Effects On Antioxidants And Membrane Leakage In Tall Fescue During Drought, C. P. West, R. D. Carson, C. A. Guerber, B. de los Reyes 2023 University of Arkansas

Endophyte Effects On Antioxidants And Membrane Leakage In Tall Fescue During Drought, C. P. West, R. D. Carson, C. A. Guerber, B. De Los Reyes

International Grassland Congress Proceedings

Tall fescue [Festuca arundinacea (Schreb.)=Lolium arundinaceum (Schreb.) S.J. Darbyshire] infected (E+) by its fungal endophyte [(Neotyphodium coenphialum Morgan-Jones & Gams.) Glenn, Bacon & Hanlin] often shows greater persistence during summer drought than endophyte-free (E-) plants (Malinowski et al., 2005). Survival of the apical meristem and growing zone of vegetative tillers likely involves biochemical adaptations whose benefits to the host are enhanced by endophyte presence. Antioxidant enzymes may scavenge free radicals during heat and drought, and thereby reduce membrane damage. Their roles in endophyte-mediated drought tolerance in tall fescue have not been tested. Our objective was to …


Nutritive Value Of Alopecurus Pratensis, Festuca Rubra, Arrhenatherum Elatius And Lolium Perenne Grown In The South Of Belgium, A. Nivyobizi, A. G. Deswysen, D. Dehareng, A. Peeters, Y. Larondelle 2023 Catholic University of Louvain, Belgium

Nutritive Value Of Alopecurus Pratensis, Festuca Rubra, Arrhenatherum Elatius And Lolium Perenne Grown In The South Of Belgium, A. Nivyobizi, A. G. Deswysen, D. Dehareng, A. Peeters, Y. Larondelle

International Grassland Congress Proceedings

In Europe, recent strategies have aimed at encouraging farmers to use production techniques more efficient in preserving the environment and maintaining natural areas. Those strategies have encouraged the use of secondary grass species in forage production systems. However, the nutritive value of those grasses is not well known. Therefore, the aim of the present study was to evaluate the energy and nitrogen values of Alopecurus pratensis (ALPR), Festuca rubra (FERU) and Arrhenatherum elatius (AREL) under moderate rates of nitrogen (N) application (60 kg N/ha per cut) and a hay-cutting regime (2 cuts/year: 25 May and 9 July). Lolium perenne cv. …


The Influence Of Management On Health Status Of Festuca Rubra In Mountain Meadows, B. Voženílková, F. Klimeš, J. Květ, Z. Mašková, B. Čermák, K. Suchý 2023 University of South Bohemia, Czech Republic

The Influence Of Management On Health Status Of Festuca Rubra In Mountain Meadows, B. Voženílková, F. Klimeš, J. Květ, Z. Mašková, B. Čermák, K. Suchý

International Grassland Congress Proceedings

Snijders & Winkelhorst (1996) investigated swards in West Europe and showed that it was not the snow mould (Microdochium nivale) but other species of the genus Fusarium (F. cerealis (Cooke) Sacc., F. graminearum Schwabe, F. culmorum (Wm. G. Sm.) Sacc. and F. acuminatum Ellis & Everh.) that caused serious damage to grasslands where Lolium perenne L. and Festuca rubra L. were dominant components. In this study the spread and harmfulness of pathogeneous fungi involved in damage to and death of some species (Festuca rubra L., Holcus mollis L.) in grass swards was examined.


Increasing The Productive Potential Of Permanent Grasslands From The Forest Steppe Area Of Romania, V. Vintu, C. Samuil, T. Iacob, St. Postolache 2023 University of Agricultural Sciences and Veterinary Medicine, Romania

Increasing The Productive Potential Of Permanent Grasslands From The Forest Steppe Area Of Romania, V. Vintu, C. Samuil, T. Iacob, St. Postolache

International Grassland Congress Proceedings

In Romania permanent grasslands represent 32 % of the total agricultural area, stretching over 4,872 million hectares, out of which 340,000 ha are located in the forest steppe area, on less productive soils, which explains their inadequate botanical composition and low quality and yields (Vintu, 2003). One of the main measures taken to increase the productivity of grasslands is through fertilisation (Birch, 1999). This paper presents the results obtained during 2000-2004 on the effect of organic fertiliser on degraded grasslands made up of Festuca valesiaca L.


Productivity Of Sahiwal And Friesian–Sahiwal Crossbreds In Marginal Grasslands Of Kenya, W. B. Muhuyi, F. B. Lukibisi, S. N. ole Sinkeet 2023 National Animal Husbandry Research Centre, Kenya

Productivity Of Sahiwal And Friesian–Sahiwal Crossbreds In Marginal Grasslands Of Kenya, W. B. Muhuyi, F. B. Lukibisi, S. N. Ole Sinkeet

International Grassland Congress Proceedings

Dual-purpose cattle can be used to exploit the production potential of semi-arid grasslands of Kenya for milk and meat production. Although the Sahiwal is adapted to these grasslands, its productivity is low. In order to increase milk and meat productivity, the Sahiwal has been crossed with the Friesian to produce Friesian-Sahiwal crossbreds (McDowell et al., 1996) adapted to the tropical environment. The objective of this study was to evaluate the productivity of Sahiwal and Friesian-Sahiwal crossbreds.


The Influence Of Harvest Period And Fertilisation On The Yield Of Some Mixed Grass And Leguminous Species Under The Forest Steppe Conditions Of North-East Romania, V. Vintu, C. Samuil, T. Iacob, St. Postolache 2023 University of Agricultural Sciences and Veterinary Medicine, Romania

The Influence Of Harvest Period And Fertilisation On The Yield Of Some Mixed Grass And Leguminous Species Under The Forest Steppe Conditions Of North-East Romania, V. Vintu, C. Samuil, T. Iacob, St. Postolache

International Grassland Congress Proceedings

In the forest steppe area of North-east Romania, temporary grasslands represent an important source of high quality fodder but they have a short period of exploitation, associated with some changes in the floristic composition (Vintu, 2003). Fertiliser application and harvest period have an important role in maintaining high productivity (Hopkins, 1991). The aim of this paper is to determine the influence of harvest period and fertilization on the yield of some grass and leguminous species in the forest steppe conditions of North-east Romania.


Yield And Mineral Concentration Changes In Maize And Italian Ryegrass Cropping Systems, Sachiko Idota, Yasuyuki Ishii 2023 University of Miyazaki, Japan

Yield And Mineral Concentration Changes In Maize And Italian Ryegrass Cropping Systems, Sachiko Idota, Yasuyuki Ishii

International Grassland Congress Proceedings

Mineral balance between plants and soil to which fertilizer has been applied is important in sustainable agriculture. Cropping systems are chosen based on considerations of crop yield, soil physical and chemical properties and climatic conditions. Thus, the sustainability of a forage cropping system should only be assessed after continuous cultivation has been practiced for several years. Forage crop production is employed in the rice paddies of Japan during summer. Thus, the objective of this study was to evaluate the yield and mineral concentration of forage crops cultivated in hard-textured soils for 4 years.


Drought Susceptibility Index; A Preferred Criterion In Screening For Tolerance In Soybean, Muhammad Zeshan Zafer, Muhammad Hammad Nadeem Tahir, Muhammad Amir Bakhtavar, Essam Darwish, Mahmood Alam Khan, Zulqurnain Khan, Chahat Fatima, Ayesha Aftab, Shifa Ur Rahman, Shoaib Ur Rehman 2023 Institute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef, University of Agriculture, Multan, Pakistan

Drought Susceptibility Index; A Preferred Criterion In Screening For Tolerance In Soybean, Muhammad Zeshan Zafer, Muhammad Hammad Nadeem Tahir, Muhammad Amir Bakhtavar, Essam Darwish, Mahmood Alam Khan, Zulqurnain Khan, Chahat Fatima, Ayesha Aftab, Shifa Ur Rahman, Shoaib Ur Rehman

Journal of Bioresource Management

Soybean (Glycine max L.) yield and yield related traits are constrained by drought. Adaptation of soybean to changing environment could be improved by exploitation and introgression of diverse germplasm in breeding program. In present study, the response to drought conditions, especially at flowering stage, was evaluated to determine the potential soybean germplasm for future soybean breeding programs in Pakistan. Field experiment was conducted under two water regimes i.e. well-water and water-limited, to assess the effect of drought in seed yield and yield related traits. Although, drought led to overall reduction of ~15 % in thousand seed weight but still …


Pollen-Mediated Gene Flow From Genetically Modified Herbicide Resistant Creeping Bentgrass, L. S. Watrud, E. H. Lee, A. Fairbrother, C. Burdick, J. R. Reichman, M. Bollman, M. Storm, G. King, P. K. van de Water 2023 US Environmental Protection Agency

Pollen-Mediated Gene Flow From Genetically Modified Herbicide Resistant Creeping Bentgrass, L. S. Watrud, E. H. Lee, A. Fairbrother, C. Burdick, J. R. Reichman, M. Bollman, M. Storm, G. King, P. K. Van De Water

International Grassland Congress Proceedings

Approximately 162 ha of multiple experimental fields of creeping bentgrass (Agrostis stolonifera L.) genetically modified for resistance to Roundup ®herbicide, were planted in central Oregon in 2002. When the fields flowered for the first time in the summer of 2003, a unique opportunity was presented to evaluate methods to monitor potential pollen-mediated gene flow from the experimental GM crop fields to compatible sentinel and resident plants that were located in surrounding, primarily non-agronomic areas.


Assessing The Risk Posed By Transgenic Virus-Resistant Trifolium Repens To Native Grasslands In Southeast Australia, R. C. Godfree, P. W. G. Chu, A. G. Young 2023 CSIRO, Australia

Assessing The Risk Posed By Transgenic Virus-Resistant Trifolium Repens To Native Grasslands In Southeast Australia, R. C. Godfree, P. W. G. Chu, A. G. Young

International Grassland Congress Proceedings

In Australia, comprehensive environmental risk assessments must be performed on transgenic plants (GMOs) prior to their commercial release. A key element is the determination of whether the release of a particular GMO poses any weediness threat to the environment or other agricultural systems, which can occur by means of direct invasion or by introgression of transgenes into wild populations of the same or closely related species. For transgenic pasture plants this question could be of added importance because many of these species have been selected for traits encouraging long-term persistence and competitiveness in complex plant communities (Godfree et al., …


Belowground Meristem Populations As Regulators Of Grassland Dynamics, Harmony J. Dalgleish, D. C. Hartnett 2023 Kansas State University

Belowground Meristem Populations As Regulators Of Grassland Dynamics, Harmony J. Dalgleish, D. C. Hartnett

International Grassland Congress Proceedings

Studies of plant populations are critical for linking organism to ecosystem-level phenomena and for understanding mechanisms driving responses to global change. In perennial grasslands, the below-ground population of meristems (the bud bank) plays a fundamental role in local plant population recruitment, persistence and dynamics. We explore two aspects of the bud bank in North American grasslands. It has been hypothesized that low variability in arid biomes is explained by meristem limitation, which constrains responses to pulses of high resource availability. Our research tests this hypothesis by comparing bud-bank populations across six sites in the United States that vary 3-fold in …


The Impact Of Vegetation Structure And Spatial Heterogeneity On Invertebrate Biodiversity Within Upland Landscapes, L. Cole, M. L. Pollock, D. Robertson, J. P. Holland, D. I. McCraken 2023 Scottish Agricultural College, UK

The Impact Of Vegetation Structure And Spatial Heterogeneity On Invertebrate Biodiversity Within Upland Landscapes, L. Cole, M. L. Pollock, D. Robertson, J. P. Holland, D. I. Mccraken

International Grassland Congress Proceedings

Livestock grazing influences vegetation structure and composition at both the patch and wider landscape scale (Milne et al., 1998), and this may have effects on upland invertebrate communities, which in turn influence bird abundance and distribution (Fuller & Gough, 1999; Cole et al., 2002). Of particular importance are open grasslands and wet flushes where invertebrates are abundant and more accessible to birds. However, there have been few studies of invertebrates associated with upland habitats, and most of these have focused on heather moorland, blanket bog, or very fine-scaled structure within grasslands (Dennis et al. 1997; 1998; 2001). This …


Towards A Comparative Map Of White Clover (Trifolium Repens) And Barrel Medic (Medicago Truncatula), M. Febrer, G. Jenkins, M. T. Abberton, D. Milbourne 2023 Teagasc, Ireland

Towards A Comparative Map Of White Clover (Trifolium Repens) And Barrel Medic (Medicago Truncatula), M. Febrer, G. Jenkins, M. T. Abberton, D. Milbourne

International Grassland Congress Proceedings

Grassland is of pivotal importance to the Irish agricultural industry. This dependence of grass is reflected in the large proportion of land area under grass, approx. 80% of the total land acreage in Ireland. The presence of white clover (Trifolium repens L.) in grassland significantly improves the overall nutritional value of the forage by increasing the relative amounts of nitrogen present. Genetic improvement of white clover through breeding of varieties should increase the productivity of grasslands. Advances in plant biotechnology offer the possibility of developing tools that will radically enhance our ability to breed improved plant varieties. The objective …


Role Of The Banyuls(Ban) Gene From Arabidopsis Thaliana In Transgenic Alfalfa Expression Of Anthocyanins And Proanthocyanidins, S. M. Hesamzadeh Hejazi, Sergio Arcioni, F. Paolocci 2023 Research Institute of Forests and Rangelands, IRAN

Role Of The Banyuls(Ban) Gene From Arabidopsis Thaliana In Transgenic Alfalfa Expression Of Anthocyanins And Proanthocyanidins, S. M. Hesamzadeh Hejazi, Sergio Arcioni, F. Paolocci

International Grassland Congress Proceedings

Condensed tannins (CTs) are flavonoid oligomers, many of which have beneficial effects on animal (bloat safe) and human health. The BAN gene encodes anthocyanidin reductase (ANR), an enzyme proposed to convert anthocyanidins to their corresponding 2,3-cis-flavan-3-ols (Xie et al., 2003). Ectopic expression of BAN in Alfalfa transgenic foliage results in accumulation of CTs. Thus, it has been assumed that the BAN gene also acts in starter units for the condensation of tannins in Alfalfa.


Clover Astra: A Web-Based Resource For Trifolium Est Analysis, G. C. Spangenberg, T. Sawbridge, E. K. Ong, C. G. Love, T. A. Erwin, E. G. Logan, D. Edwards 2023 La Trobe University, Australia

Clover Astra: A Web-Based Resource For Trifolium Est Analysis, G. C. Spangenberg, T. Sawbridge, E. K. Ong, C. G. Love, T. A. Erwin, E. G. Logan, D. Edwards

International Grassland Congress Proceedings

White clover (Trifolium repens L.) is a major temperate forage legume.


Controlled Flowering Project For Lolium Perenne At Agresearch: An Overview, I. Kardailsky, B. Veit, N. Forester, M. Gagic, K. Richardson, Marty J. Faville, G. Bryan 2023 AgResearch, New Zealand

Controlled Flowering Project For Lolium Perenne At Agresearch: An Overview, I. Kardailsky, B. Veit, N. Forester, M. Gagic, K. Richardson, Marty J. Faville, G. Bryan

International Grassland Congress Proceedings

Ryegrass (Lolium perenne) is an important forage crop in New Zealand. The work presented here has the goal of developing a system for complete and arbitrary control of the transition from vegetative to floral development. For this, we have pursued an integrated approach utilising genomics with both forward and reverse genetics. Like other model plants, photoperiodic and vernalization pathways are presumed to be operating in ryegrass and control the activity of the meristem identity/floral patterning genes. The candidate gene approach targeting the photoperiodic pathway is described in an accompanying abstract (Gagic et al.). Other candidate genes include …


Monitoring Of Gene Expression Profiles And Identification Of Candidate Genes Involved In Drought Tolerance In Festuca Mairei With Cdna-Aflp, J. P. Wang, S. S. Bughrara 2023 Michigan State University

Monitoring Of Gene Expression Profiles And Identification Of Candidate Genes Involved In Drought Tolerance In Festuca Mairei With Cdna-Aflp, J. P. Wang, S. S. Bughrara

International Grassland Congress Proceedings

Drought stress is one of the most complex environmental constraints on plants. Response of plant to drought stress is manifested by various changes in physiological and metabolic processes, which are reflected at the molecular level. cDNA-amplified fragment length polymorphism (cDNA-AFLP) is a high-throughput transcript profiling technique for temporal and spatial gene expression analysis (Bachem et al., 1996). To understand the molecular genetic basis of drought tolerance of grasses, we applied the cDNA-AFLP procedure to identify the genes responding to drought stress of Festuca mairei (Fm), which showed a xeriphytic adaptation (Marlatt et al., 1997).


Gene Discovery And Molecular Dissection Of Fructan Metabolism In Perennial Ryegrass (Lolium Perenne), J. Chalmers, A. Lidgett, X. Johnson, K. Terdich, N. Cummings, Y. Y. Cao, K. Fulgueras, M. Emmerling, T. Sawbridge, E. K. Ong, A. Mouradov, G. C. Spangenberg 2023 La Trobe University, Australia

Gene Discovery And Molecular Dissection Of Fructan Metabolism In Perennial Ryegrass (Lolium Perenne), J. Chalmers, A. Lidgett, X. Johnson, K. Terdich, N. Cummings, Y. Y. Cao, K. Fulgueras, M. Emmerling, T. Sawbridge, E. K. Ong, A. Mouradov, G. C. Spangenberg

International Grassland Congress Proceedings

Fructans are the main soluble carbohydrate stored in up to a third of the vegetation of the earth, including the economically important temperate grasses. Fructans are polymers of fructose attached to a sucrose precursor. Perennial ryegrass (L. perenne L.) accumulates fructans of the inulin series, inulin neoseries and levan neoseries. Four enzymes are required to produce fructans of this profile: 1-SST (sucrose:sucrose 1- fructosyltransferase), 1-FFT (fructan:fructan 1-fructosyltransferase), 6G-FFT (6-glucose fructosyltransferase) and 6-FFT (fructan:fructan 6-fructosyltransferase) or 6-SFT (sucrose:fructan 6-fructosyltransferase) (Figure 1). Fructan biosynthetic enzymes have evolved from invertases and thus it is argued that fructan metabolism is an extension …


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