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Genetic Analysis Of Anthocyanin Content In Purple Waxy Corn (Zea Mays L. Var. Ceratina Kulesh) Kernel And Cob, B. Harakotr, B. Suriharn, K. Lertrat, M. P. Scott 2017 Thammasat University

Genetic Analysis Of Anthocyanin Content In Purple Waxy Corn (Zea Mays L. Var. Ceratina Kulesh) Kernel And Cob, B. Harakotr, B. Suriharn, K. Lertrat, M. P. Scott

M. Paul Scott

Anthocyanins of pigmented corn become a topic of increasing interest recently due to consumer awareness of their various health benefits. A better understanding on the gene effects to anthocyanin is an important for high antioxidants breeding programs. The objective of the present study was to determine gene effect controlling of anthocyanin content in purple waxy corn kernel and cob by generation mean analysis. For the cross of KND 10-4P (P1) × BW (P2), F2, and backcross generations were developed. All generations were planted in the randomized complete block design (RCB) with 3 replications at the Khon Kaen University, Thailand. Genetic analysis ...


Phenotypic Characterization Of Inbred Lines And Their O2 Conversions, M. P. Scott 2017 United States Department of Agriculture

Phenotypic Characterization Of Inbred Lines And Their O2 Conversions, M. P. Scott

M. Paul Scott

Peter Loesh was a plant breeder at Iowa State University from 1971 to 1980. During this time, he worked extensively with the o2 mutation in an effort to improve the nutritional quality of maize. Unfortunately, he passed away suddenly, and this work was not brought to a conclusion. Some of his effort was devoted to developing o2 back-cross conversions of public domain inbred lines. Because Dr. Loesh’s field books have been lost, the extent of recovery of the recurrent parent phenotype is not known.


Tissue-Print Immunodetection Of Transgene Products In Endosperm For High-Throughput Screening Of Seeds, M. Paul Scott 2017 United States Department of Agriculture

Tissue-Print Immunodetection Of Transgene Products In Endosperm For High-Throughput Screening Of Seeds, M. Paul Scott

M. Paul Scott

This method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.


Comparison Of Tungsten Carbide And Stainless Steel Ball Bearings For Grinding Single Maize Kernels In A Reciprocating Grinder, Jay Hoch, Adrienne Moran Lauter, M. Paul Scott 2017 Iowa State University

Comparison Of Tungsten Carbide And Stainless Steel Ball Bearings For Grinding Single Maize Kernels In A Reciprocating Grinder, Jay Hoch, Adrienne Moran Lauter, M. Paul Scott

M. Paul Scott

When grinding many corn kernels at once, there are several different options to choose from, but single kernel grinding is limited to few options with relatively low throughput. An attractive alternative is a reciprocating grinder with 3/8” grinding balls and a 4ml polycarbonate vial set. This format allows grinding of 24 samples simultaneously, which is a significant improvement to grinding throughput. A reciprocating grinder like the Talboys HT Homogenizer (Troemner, Thorofare, NJ), operates by violently shaking the 4ml polycarbonate vials containing a bearing and a single kernel. In our experience, stainless steel ball bearings do not lead to a ...


Allelism Testing Of Maize Coop Stock Center Lines Containing Unknown Brown Midrib Alleles, L. J. Haney, S. Hake, M. P. Scott 2017 United States Department of Agriculture

Allelism Testing Of Maize Coop Stock Center Lines Containing Unknown Brown Midrib Alleles, L. J. Haney, S. Hake, M. P. Scott

M. Paul Scott

This report summarizes the allele testing of brown midrib stocks characterized by phenotype only in the Maize Coop Stock Center collection. Maize seeds obtained from the Maize Coop Stock Center were planted in the summer nursery of 2006 and 2007 at the Agronomy Farms of Iowa State University (Boone County, IA). Each of the lines containing a homozygous recessive unknown brown midrib allele was crossed to a series of lines, each containing a different homozygous recessive known brown midrib allele. The F1 plants were screened for reddishbrown pigmentation in the leaf midrib at the V8 stage of growth. Presence of ...


Effect Of Treatment And Cultivar On The Ensiling Of Corn Stover, R. E. Muck, J. G. Coors, T. L. Richard, M. P. Scott 2017 United States Department of Agriculture

Effect Of Treatment And Cultivar On The Ensiling Of Corn Stover, R. E. Muck, J. G. Coors, T. L. Richard, M. P. Scott

M. Paul Scott

Nine cultivars of corn stover selected for ethanol potential were harvested (34 to 40% dry matter) and each ensiled with six treatments: untreated, lactic acid bacteria, cell-wall degrading enzymes, sulfuric acid, bacteria-enzyme combination and enzyme-acid combination. Ensiling was carried out in vacuum-sealed bags at ~22°C for 60 d. The untreated stovers ensiled well. Lactic acid bacteria and enzyme treatments had no effect on pH, but the bacteria-enzyme combination lowered pH in some cultivars. The acid and acid-enzyme treatments had low pH values ranging from 1.3 to 1.5. Lactic acid was generally highest in the bacteria-enzyme treatment whereas ...


A Comparison Of Iowa's Original Corn Suitability Rating To The New Corn Suitability Rating 2, Aaron M. Sassman, C. Lee Burras, Gerald A. Miller 2017 Iowa State University

A Comparison Of Iowa's Original Corn Suitability Rating To The New Corn Suitability Rating 2, Aaron M. Sassman, C. Lee Burras, Gerald A. Miller

C. Lee Burras

In 1971 Dr. Thomas Fenton and several colleagues at Iowa State University (ISU) developed the Corn Suitability Rating (CSR) providing producers and assessors a soil productivity index for Iowa. The CSR for a soil is based on its inherent soil properties to a depth of 152- to 203-cm, mean weather data for a 30-yr period, and its potential for row crop production. In 1984 CSR data was combined with existing Soil Conservation Service (SCS) form SOILS-5 data, Map Unit Use File (MUUF), and Iowa CONS-9 data to create a single computer file of compiled soils data called the Iowa Soil ...


Corn Suitability Rating 2 (Csr2) Equation And Component Values, C. L. Burras, G. A. Miller, T. E. Fenton, A. M. Sassman 2017 Iowa State University

Corn Suitability Rating 2 (Csr2) Equation And Component Values, C. L. Burras, G. A. Miller, T. E. Fenton, A. M. Sassman

C. Lee Burras

CSR2 is a corn suitability rating calculated using six parameters. The “perfect corn producing” soil gets a rating of 100. A soil having nearly no potential to grow corn receives a rating of 5. Most soils have ratings somewhere in between. CSR2 is calculated on a map unit (MU) basis using the maps and data of the Iowa Cooperative Soil Survey (ICSS). Since the ICSS includes several agencies with sometimes slightly different data there are two ways it can be calculated. The first is CSR2-ISU, which assumes a MU is the soil series listed in the MU name. The second ...


Species Diversity And Functional Composition Of Pastures That Vary In Landscape Position And Grazing Management, John A. Guretzky, Kenneth J. Moore, E. Charles Brummer, C. Lee Burras 2017 United States Army Corps of Engineers

Species Diversity And Functional Composition Of Pastures That Vary In Landscape Position And Grazing Management, John A. Guretzky, Kenneth J. Moore, E. Charles Brummer, C. Lee Burras

C. Lee Burras

The productivity of grasslands depends in part on their diversity of species and functional composition. Our objective was to examine the effects of three landscape positions (summit, backslope, and toeslope) and three stocking systems (continuous, rotational, and nongrazed) on species diversity and percentage of cover of grass, legume, and weed species functional types in southeastern Iowa pastures. Data were collected in 0.2-m2 plots randomly distributed throughout each of four replicate pastures in spring and summer 2000 and 2001. Backslope landscape positions within pastures managed with either continuous or rotational stocking contained the greatest overall diversity of species. Across years ...


Soil Profile Transformation After 50 Years Of Agricultural Land Use, Jessica J. Veenstra, C. Lee Burras 2017 Iowa State University

Soil Profile Transformation After 50 Years Of Agricultural Land Use, Jessica J. Veenstra, C. Lee Burras

C. Lee Burras

Despite a large body of scientific research that shows that soils change on relatively short time scales under different management regimes, classical pedological theory states that we should expect these changes to occur only in the surface few centimeters and that they are not of adequate magnitude to suggest fundamental changes in pedon character over short periods of time. In fact, rarely, do the scientists that make these comparisons report on any properties deeper than 30 to 45 cm in the soil profile. With this study, we evaluate soil transformation to a depth of 150 cm after 50 yr of ...


History Of East European Chernozem Soil Degradation; Protection And Restoration By Tree Windbreaks In The Russian Steppe, Yury G. Chendev, Thomas J. Sauer, Guillermo Hernandez Ramirez, Charles Lee Burras 2017 Belgorod State University

History Of East European Chernozem Soil Degradation; Protection And Restoration By Tree Windbreaks In The Russian Steppe, Yury G. Chendev, Thomas J. Sauer, Guillermo Hernandez Ramirez, Charles Lee Burras

C. Lee Burras

The physiographic region of the Central Russian Upland, situated in the Central part of Eastern Europe, is characterized by very fertile grassland soils—Chernozems (Mollisols in the USDA taxonomy). However, over the last several centuries this region has experienced intense land-use conversion. The most widespread and significant land-use change is the extensive cultivation of these soils. As a result, Chernozems of the region that were some of the most naturally fertile soils in the world with thick A horizons had become, by the second half of the 19th century, weakly productive, with decreased stocks of organic matter. When not protected ...


Application Of Silicon Ameliorated Salinity Stress And Improved Wheat Yield, M. A. Ibrahim, A. M. Merwad, E. A. Elnaka, C. L. Burras, L. Follett 2017 Iowa State University

Application Of Silicon Ameliorated Salinity Stress And Improved Wheat Yield, M. A. Ibrahim, A. M. Merwad, E. A. Elnaka, C. L. Burras, L. Follett

C. Lee Burras

Management of soil salinity is an important research field around the globe, especially when associated with the limited water resources. This work aimed to improve the growth and yield of wheat (Triticum aestivum L. CV. Sakha-93) grown under salinity stress. A completely randomized design pot experiment with three replications was conducted in a loamy soil with various levels of salinity under local weather conditions. The treatments included five levels of salinity (2.74, 5.96, 8.85, 10.74, and 13.38 dSm-1) prepared by adding NaCl to the selected soil and five treatments of Si (0, 2.1, 4 ...


Effectiveness Of Foliar Fungicides By Timing On Northern Leaf Blight On Hybrid Corn In Northeast Iowa, Alison Robertson, Ken Pecinovsky 2017 Iowa State University

Effectiveness Of Foliar Fungicides By Timing On Northern Leaf Blight On Hybrid Corn In Northeast Iowa, Alison Robertson, Ken Pecinovsky

Alison Robertson

Fungicide use on hybrid corn continues to be of interest to many farmers in Iowa. The number of fungicides registered for use on corn continues to increase, especially with the introduction of various generics. The objectives of this project were to assess the effect of timing of application of fungicides on disease, evaluate the yield response of hybrid corn to foliar fungicide application, and discern differences, if any, between fungicide products.


Effectiveness Of Foliar Fungicides By Timing On Northern Leaf Blight On Hybrid Corn In Northern Iowa, Alison Robertson, Matt Schnabel 2017 Iowa State University

Effectiveness Of Foliar Fungicides By Timing On Northern Leaf Blight On Hybrid Corn In Northern Iowa, Alison Robertson, Matt Schnabel

Alison Robertson

Fungicide use on hybrid corn continues to be of interest to many farmers in Iowa. The number of fungicides registered for use on corn continues to increase, especially with the introduction of various generics. The objectives of this project were to assess the effect of timing of application of fungicides on disease, evaluate the yield response of hybrid corn to foliar fungicide application, and discern differences, if any, between fungicide products.


Effectiveness Of Foliar Fungicides By Timing On Northern Leaf Blight And Common Rust On Hybrid Corn, Alison Robertson, Chad Huffman, Josh Sievers 2017 Iowa State University

Effectiveness Of Foliar Fungicides By Timing On Northern Leaf Blight And Common Rust On Hybrid Corn, Alison Robertson, Chad Huffman, Josh Sievers

Alison Robertson

Fungicide use on hybrid corn continues to be of interest to many farmers in Iowa. The number of fungicides registered for use on corn continues to increase, especially with the introduction of various generics. The objectives of this project were to assess the effect of timing of application of fungicides on disease, evaluate the yield response of hybrid corn to foliar fungicide application, and discern differences, if any, between fungicide products.


2015 Evaluation Of Commercial Seed Treatments On Soybean At Three Locations In Iowa, Alison E. Robertson, Mauricio Serrano, Stith N. Wiggs 2017 Iowa State University

2015 Evaluation Of Commercial Seed Treatments On Soybean At Three Locations In Iowa, Alison E. Robertson, Mauricio Serrano, Stith N. Wiggs

Alison Robertson

In 2015, 13 currently available commercial seed treatments were evaluated at three locations in Iowa. Seed treatments that reduce the risk of seedling diseases, soybean cyst nematode (SCN) and sudden death syndrome (SDS) were included in the trial. The goal of the trials is to provide information of the effect of commercial seed treatments on stand count, disease incidence and severity, and yield of soybean. Funding is provided by Iowa Soybean Association.


Comparison Of Commercial Seed Treatments On Soybean In Iowa, Mauricio Serrano, Alison Robertson, Myron Rees 2017 Iowa State University

Comparison Of Commercial Seed Treatments On Soybean In Iowa, Mauricio Serrano, Alison Robertson, Myron Rees

Alison Robertson

The use of seed treatments on soybean is becoming more prevalent in Iowa. Due to increasing seed costs, many farmers are reducing their seeding rates, consequently stand establishment is very important. Seed treatment products may include fungicides, nematicides, insecticides, and biologicals. Seed treatments protect stand by reducing risk of seedling disease and early-season insect damage. The objectives of this study were to evaluate commercial seed treatments for stand establishment, disease and insect control, and effect on yield.


Phenotypic And Genotypic Diversity Of Eastern Leatherwood In Five Populations That Span Its Geographic Distribution, Bryan J. Peterson, William R. Graves, Jyotsna Sharma 2017 Iowa State University

Phenotypic And Genotypic Diversity Of Eastern Leatherwood In Five Populations That Span Its Geographic Distribution, Bryan J. Peterson, William R. Graves, Jyotsna Sharma

William R Graves

Knowledge of variation among populations is important for making decisions about conservation of rare, threatened or endangered taxa. We investigated variation within and among five populations ofDirca palustris L. an understory shrub with a sporadic distribution and life-history traits that led us to predict strong phenotypic and genetic differentiation characteristic of historically limited gene flow. Populations sampled in Florida, Alabama, Illinois, Wisconsin and North Dakota spanned the geographic distribution of the species. Phenotypic traits differed among populations, and many traits correlated with latitude and environmental characteristics of the five sites. Principal components (PC) analysis showed that the first three PCs ...


Confirmation That Dirca Spp. (Thymelaeaeae) Reproduce From Rhizomes, William R. Graves 2017 Iowa State University

Confirmation That Dirca Spp. (Thymelaeaeae) Reproduce From Rhizomes, William R. Graves

William R Graves

There are three North American species of deciduous shrubs in the genus Dirca L. Dirca palustris L. occurs in the eastern United States and in southeastern Canada (Vogelmann 1953). Although its distribution is broad, D. palustris typically is obscure locally and is restricted to moist soils as an understory species along wooded slopes (Kurz 1997). Dirca occidentalis Gray also occurs on slopes (McMinn and Forderhase 1935), but its distribution is narrowly restricted to six counties of California near the San Francisco Bay. Within this area, D. occidentalis inhabits cool niches with frequent fog and relatively uniform soil moisture (Johnson 1994 ...


Function And Biodegradation In Soil Of Bioplastic Horticultural Containers Made Of Pla-Biorestm Composites, James Schrader, Kenneth McCabe, William Graves, David Grewell 2017 Iowa State University

Function And Biodegradation In Soil Of Bioplastic Horticultural Containers Made Of Pla-Biorestm Composites, James Schrader, Kenneth Mccabe, William Graves, David Grewell

William R Graves

Container-crops horticultural industries rely almost exclusively on petroleum-based plastic containers for modern production systems. Containers made of these materials fulfill all of the functions required during crop production, and perform better than containers made of clay, peat, and other natural materials, but the source of the plastic materials (fossil carbon), their lack of biodegradability, and their end-of-life disposal (97% end up in landfills) are major obstacles to sustainability. Although function and efficiency are among the most important aspects in determining the best materials for horticultural containers, there is no need for containers to persist in the environment for decades when ...


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