Production Of A Gonadotropin-Releasing Hormone 2 Receptor Knockdown (Gnrhr2 Kd) Swine Line., 2017 University of Nebraska-Lincoln
Production Of A Gonadotropin-Releasing Hormone 2 Receptor Knockdown (Gnrhr2 Kd) Swine Line., Amy T. Desaulniers, Rebecca Cederberg, Ginger A. Mills, Clay A. Lents, Brett R. White
Faculty Papers and Publications in Animal Science
Swine are the only livestock species that produce both the second mammalian isoform of gonadotropin-releasing hormone (GNRH2) and its receptor (GNRHR2). Previously, we reported that GNRH2 and GNRHR2 mediate LH-independent testosterone secretion from porcine testes. To further explore this ligand-receptor complex, a pig model with reduced GNRHR2 expression was developed. Small hairpin RNA sequences targeting porcine GNRHR2 were subcloned into a lentiviral-based vector, lentiviral particles were generated and microinjected into the perivitelline space of zygotes, and embryos were transferred into a recipient. One GNRHR2 knockdown (KD) female was born that subsequently produced 80 piglets from 6 litters with 46 hemizygous ...
Designing An Authentic Behavioral Research Experience In The Classroom Using Siamese Fighting Fish, Betta Splendens, 2017 Abraham Lincoln High School
Designing An Authentic Behavioral Research Experience In The Classroom Using Siamese Fighting Fish, Betta Splendens, Gianne Souza, Ronald M. Coleman
STAR (STEM Teacher and Researcher) Presentations
Recently there have been major shifts in biology education towards emphasizing the teaching of the scientific process to more closely reflect actual scientific practices. Authentic research experiences require the development of many higher order cognitive skills. Creating an authentic research experience that appropriately scaffolds the development of these complex skills while engaging individual student motivation remains challenging in classroom settings. Some common challenges include limited time available for working with large numbers of students, cramped working areas, and limited funding and materials resources. Using the Siamese Fighting Fish, Betta splendens, we have created an economically viable authentic animal behavioral research ...
Capacitive Memory Alters Alternans And Spontaneous Activity In A Minimal Cardiomyocyte Model, 2017 Virginia Commonwealth University
Capacitive Memory Alters Alternans And Spontaneous Activity In A Minimal Cardiomyocyte Model, Tien Comlekoglu, Seth H. Weinberg
Biology and Medicine Through Mathematics Conference
No abstract provided.
Growth Patterns And Size-Group Interactions In Hatchery-Reared Age-0 Lake Sturgeon, 2017 Northern Michigan University
Growth Patterns And Size-Group Interactions In Hatchery-Reared Age-0 Lake Sturgeon, Joseph M. Susco
All NMU Master's Theses
Raising lake sturgeon (Acipenser fulvescens) in hatcheries is an important tool for rehabilitation and conservation, but is expensive. When hatchery sturgeon do not grow large enough before stocking, their odds of survival decline significantly and resources are wasted. We investigated the potential limiting effect of large lake sturgeon on the growth rate of smaller conspecifics housed in the same tank, and the possibility of increasing growth rates by separating individuals into different size classes. We also examined plasma cortisol levels of the different size classes over time as an indicator of stress levels, and levels of free plasma thyroid hormones ...
Undergraduate Research On General Aviation Hypoxia: A Student's Perspective, 2017 Embry-Riddle Aeronautical University
Undergraduate Research On General Aviation Hypoxia: A Student's Perspective, Timothy B. Holt, Jacqueline Luedtke, Claire G. Schindler
The problem this study addresses is the uncertainty of the common circumstances that general aviation pilots find themselves in that create a hypoxic state, as well as whether or not that pilot reported the occurrence to the proper establishments. The results of this study showed not only those that were impacted the greatest by hypoxia, but also a caring concern for reporting these events. The key elements for this research were:
• Level of flying experience that encountered hypoxia
• Reporting statistics
• Reasons for not reporting the event
• Suggestions for bettering flight physiology training
The Effect Of Incline On Caloric Expenditure Measured By A Wrist-Worn Commercial Activity Monitor, 2017 James Madison University
The Effect Of Incline On Caloric Expenditure Measured By A Wrist-Worn Commercial Activity Monitor, Kaitlin M. Bickel, Trent Hargens, Christopher Womack, Elizabeth Edwards
Senior Honors Projects, 2010-current
Purpose Physical activity monitors have become popular among the general public to monitor steps, floors climbed, active minutes, and energy expenditure (EE). While there is evidence to support that these devices are accurate in counting steps, there is limited and inconclusive research regarding how accurate they are in measuring EE. This study aimed to test the accuracy of a newer commercial physical activity monitor, the Fitbit Charge (FC), in reporting EE compared to a research-grade accelerometer (GT3X), and indirect calorimetry (IC) while walking on a treadmill with and without incline.
Methods 30 subjects (22 female and 8 male) walked on ...
Impacts Of Long-Term Precipitation Manipulation On Hydraulic Architecture, Xylem Function, And Canopy Status In A Piñon-Juniper Woodland, Patrick J. Hudson
The Southwestern US is predicted to become hotter and drier, as global climate change forces increasing temperatures and variability in timing and size of precipitation inputs. Drought stress has become more frequent in recent decades, and resulted in massive forest mortality in piñon-juniper woodlands. During recent severe droughts (2000-2003, 2009-2012), piñon pine (Pinus edulis Englem.) suffered disproportionately high mortality compared to co-occurring one-seed juniper (Juniperus monosperma [Engelm.] Sarg.). A large-scale precipitation manipulation experiment was established in a piñon-juniper woodland in central New Mexico to test hypotheses regarding tree survival and mortality with respect to altered water regimes. Our treatments consisted ...
Examining The Combined Effects Of Dissolved Oxygen, Temperature, And Body Size On The Physiological Responses Of A Model Macrobenthic Polychaete Species, Capitella Teleta, 2016 University of Southern Mississippi
Examining The Combined Effects Of Dissolved Oxygen, Temperature, And Body Size On The Physiological Responses Of A Model Macrobenthic Polychaete Species, Capitella Teleta, Kelsey Burns Gillam
While the scientific community is in consensus that coastal systems are threatened by climate change, few climate change studies test the effects of more than one variable directly related to climate change. The dissolved oxygen (DO) levels of the ocean are currently subject to both global warming and eutrophication; 94% of all hypoxia zones are expected to experience >2°C increase by 2035. This dissertation aims to examine how a model organism responds to simultaneous thermal and DO stress involving four levels of DO (100%, 70%, 50%, and 20%) saturation and three temperatures (15°C, 20°C, and 25°C ...
Follicular Dynamics In Insulin Resistant Mares, 2016 University of Tennessee, Knoxville
Follicular Dynamics In Insulin Resistant Mares, Julio Cesar Prado
Obesity and insulin resistance have been linked to prolonged interovulatory period, aberrations in the estrous cycle, and continuous reproductive activity during the non-breeding season. EMS has been determined to influence the intrafollicular environment of mare ovaries. In humans, insulin resistance has been linked to polycystic ovaries as part of Polycystic Ovarian Syndrome (PCOS). A study was conducted to determine 1) the impact of insulin resistance on follicle growth and size at ovulation, and 2) whether predicted ovulatory follicles respond to hCG administration in Insulin-resistant (IR) mares. Mares were selected for the study based on insulin sensitivity and separated into an ...
Early Nutritional Programming To Enhance The Utilization Of Plant Based Diets In Fish (Largemouth Bass, Micropterus Salmoides), 2016 Kentucky State University
Early Nutritional Programming To Enhance The Utilization Of Plant Based Diets In Fish (Largemouth Bass, Micropterus Salmoides), Maya Rene Jackson, Cora Teets, Amit Kumar Yadav, Waldemar Rossi
Aquaculture has been the fastest growing animal food-producing sector for over half a century. Sustainable aquaculture should include replacement of fish-based feed sources by plant-based ingredients. However, this approach is often impeded by poor growth in carnivorous fish such as largemouth bass (LMB, Micropterus salmoides) fed high levels of plant-based protein. Therefore, our overall goal of the present work was to develop alternative methods of utilizing the plant-based diets (PBD) for sustainable LMB production. When fish is exposed to PBD early in their life, they may accept them more efficiently at later stages (so called Nutritional Programing, NP). Therefore, we ...
A 2-D Compartmental Model For Multi-Capillary Supply, 2016 Georgia Gwinnett College
A 2-D Compartmental Model For Multi-Capillary Supply, Liang Sun, Junkoo Park, Alessandra L. Barrera
Annual Symposium on Biomathematics and Ecology: Education and Research
No abstract provided.
Effects Of S1p Mutation On Er Stress And Cholesterol Synthesis Markers In Human Epithelial Cells, 2016 Washington University in St. Louis
Effects Of S1p Mutation On Er Stress And Cholesterol Synthesis Markers In Human Epithelial Cells, Connie Gan, George G. Schweitzer, Rita T. Brookheart, Robert C. Bucelli, Brian N. Finck
Undergraduate Research Symposium Posters
Site-1 Protease (S1P) is a Golgi-resident enzyme required for activation and subsequent nuclear localization of several major transcription factors. A 24-year-old female patient with a de novo single point mutation in S1P presented with a complex phenotype of gut hypomotility, abnormal optic nerves, and polycystic ovarian syndrome. Furthermore, this patient suffers from phenotypes related to skeletal muscle dysfunction, resulting in decreased ability to perform physical exercise due to unusual post-exercise pain, fatigue, nausea, and vomiting. This phenomenon has been described in the literature to manifest from myoedema and the breakdown of muscle that releases intracellular proteins into the blood, or ...
Growth And Productivity Of Irrigated Rice (Oryza Sativa L.) For A Tropical High Altitude Environment In Rwanda, 2016 University of Arkansas, Fayetteville
Growth And Productivity Of Irrigated Rice (Oryza Sativa L.) For A Tropical High Altitude Environment In Rwanda, Elie Rene Gasore
Theses and Dissertations
Yield components, grain yield, biomass and plant N accumulation, and N fertilizer responses of irrigated rice (Oryza sativa, L.) were evaluated for a tropical inland valley environment in the high altitudes in Rwanda. Effects were measured for nitrogen (N) fertilizer rate (0, 60, 90,120,150,180 kg N ha-1), season, growth stage and cultivar of different subspecies and plant types during 2012 dry season (DS) and 2013 wet season (WS) at Cyili Rice Research Farm (1380 m above sea level). Variations in irradiance (17.2± 0.32 MJ m-2 day-1 in DS and 9.4 ± 0.66 MJ m-2 ...
Designing A Mobile Space Habitat Analog, 2016 Embry-Riddle Aeronautical University - Daytona Beach
Designing A Mobile Space Habitat Analog, Victor Kitmanyen, Matthew Burkhard, Timothy Disher
Human Factors and Applied Psychology Student Conference
No abstract provided.
Photosynthetic Acclimation To Warming And Elevated Co2 In Two Antarctic Vascular Plant Species, 2016 The University of Western Ontario
Photosynthetic Acclimation To Warming And Elevated Co2 In Two Antarctic Vascular Plant Species, Vi Nt Bui
Electronic Thesis and Dissertation Repository
Climate change can affect the performance of the only two vascular plant species found in Antarctica, Deschampsia antarctica and Colobanthus quitensis. I investigated the response of these two species to warming and elevated CO2 in terms of photosynthesis and leaf anatomy. While photosynthesis increased directly with rising temperature and CO2, it showed no acclimation to changes in growth temperature, and a small degree of acclimation to growth under elevated CO2. Likewise, leaf anatomy displayed little plasticity in response to changes in the growth environment, although D. antarctica’s stomatal groove structure was modified under warming, likely to ...
Long-Term, High Frequency In Situ Measurements Of Intertidal Mussel Bed Temperatures Using Biomimetic Sensors, 2016 Northeastern University
Long-Term, High Frequency In Situ Measurements Of Intertidal Mussel Bed Temperatures Using Biomimetic Sensors, Brian Helmuth, Francis Choi, Allison Matzelle, Jessica Torossian, Scott Morello, K.A.S. Mislan, Lauren Yamane, Denise Strickland, P. Szathmary, Sarah Gilman, Alyson Tockstein, Thomas Hilbish, Michael Burrows, Anne Marie Power, Elizabeth Gosling, Nova Mieszkowska, Christopher Harley, Michael Nishizaki, Emily Carrington, Bruce Menge, Laura Petes, Melissa Foley, Angela Johnson, Megan Poole, Mae Noble, Erin Richmond, Matt Robart, Jonathan Robinson, Jerod Sapp, Jackie Sones, Bernardo Broitman, Mark Denny, Katharine Mach, Luke P. Miller, Michael O'Donnell, Philip Ross, Gretchen Hofmann, Mackenzie Zippay, Carol Blanchette, J. Macfarlan, Eugenio Carpizo-Ituarte, Benjamin Ruttenberg, Carlos Peña Mejía, Christopher Mcquaid, Justin Lathlean, Cristián Monaco, Katy Nicastro, Gerardo Zardi
At a proximal level, the physiological impacts of global climate change on ectothermic organisms are manifest as changes in body temperatures. Especially for plants and animals exposed to direct solar radiation, body temperatures can be substantially different from air temperatures. We deployed biomimetic sensors that approximate the thermal characteristics of intertidal mussels at 71 sites worldwide, from 1998-present. Loggers recorded temperatures at 10–30 min intervals nearly continuously at multiple intertidal elevations. Comparisons against direct measurements of mussel tissue temperature indicated errors of ~2.0–2.5 °C, during daily fluctuations that often exceeded 15°–20 °C. Geographic patterns in ...
Changes In Brain Water Content In The Rainbow Trout (Oncorhynchus Mykiss) And In The Goldfish (Carassius Auratus) Due To High External Ammonia Exposure, Phillip Q.H. Pham-Ho
Theses and Dissertations (Comprehensive)
In fishes, hyperammonemia may occur following feeding or exposure to abnormally high concentrations of environmental ammonia due to sewage effluents, agricultural run-off and in crowded aquaculture pens. Increased internal ammonia can result in hyperactivity, convulsions, coma and death. In mammals, it is also associated with potentially fatal brain edema, in which the accumulation of intracellular water results in swelling, increased intracranical pressure and herniation leading to death. Recently it was shown that rainbow trout (Oncorhynchus mykiss) and goldfish (Carassius auratus) experience brain swelling following exposure to high external ammonia (HEA). However, the mechanism of ammonia-induced brain swelling in fishes remains ...
Sensory Modalities Underlying The Escape Response Of The Cricket, Acheta Domesticus, To Looming Stimuli, 2016 James Madison University
Sensory Modalities Underlying The Escape Response Of The Cricket, Acheta Domesticus, To Looming Stimuli, Ariel M. Childs
Senior Honors Projects, 2010-current
In order to prevent injury or capture by a predator, animals have evolved escape behavior. Despite offering a more realistic, multimodal, approximation of an approaching predator, looming stimuli have rarely been used to evoke escape behavior in crickets. Wind stimuli, however, have been used on a variety of insects, including crickets where it has been found that direction of escape is directly correlated to the angle of incoming wind stimuli. Wind stimuli are detected by sensilla trichodea, small filiform hairs covering the cerci of crickets, locusts and cockroaches. Despite having other complex sensory systems, such as antennae and vision, little ...
Success Stories And Emerging Themes In Conservation Physiology, 2016 University of Windsor
Success Stories And Emerging Themes In Conservation Physiology, Christine L. Madliger, Steven J. Cooke, Erica J. Crespi, Jennifer L. Funk, Kevin R. Hultine, Kathleen E. Hunt, Jason R. Rohr, Brent J. Sinclair, Cory D. Suski, Craig K. R. Willis, Oliver P. Love
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
The potential benefits of physiology for conservation are well established and include greater specificity of management techniques, determination of cause–effect relationships, increased sensitivity of health and disturbance monitoring and greater capacity for predicting future change. While descriptions of the specific avenues in which conservation and physiology can be integrated are readily available and important to the continuing expansion of the discipline of ‘conservation physiology’, to date there has been no assessment of how the field has specifically contributed to conservation success. However, the goal of conservation physiology is to foster conservation solutions and it is therefore important to assess ...
Longevity Of Mineral Supplements Within The Soil And Associated Use By White-Tailed Deer, 2015 University of Nebraska Kearney
Longevity Of Mineral Supplements Within The Soil And Associated Use By White-Tailed Deer, Brian C. Peterson, Keith D. Koupal, Andrew K. Schissel, Cody M. Siegel
Transactions of the Nebraska Academy of Sciences and Affiliated Societies
Humans have baited wildlife such as white-tailed deer (Odocoileus virginianus) for generations with the primary purpose of increasing hunting harvest success. Baiting regulation changes are often considered by state management agencies as they pertain to hunting opportunity, fair chase, and disease risk. Cervids require a variety of minerals to supplement biological processes, especially sodium (Na), calcium (Ca), and phosphorus (P). We developed artificial mineral supplement sites set in front of trail cameras to monitor deer use. Pooled soil samples were collected at mineral sites and compared to the surrounding area to determine the longevity of elevated minerals levels within the ...