Anti-Factor Xa Level Monitoring For Enoxaparin Prophylaxis And Treatment In High-Risk Patient Groups, 2023 HCA Florida North Florida Hospital
Anti-Factor Xa Level Monitoring For Enoxaparin Prophylaxis And Treatment In High-Risk Patient Groups, Lucie Sikes, Kipson Charles, Abigail Antigua, Rima Patel, Selina Imboywa, Pheba Cherian
HCA Healthcare Journal of Medicine
Monitoring anti-factor Xa levels is a controversial topic in the inpatient setting due to resource utilization and unclear conditional guideline recommendations regarding this practice. Enoxaparin dosing in certain high-risk patient populations such as those with low body weight, obesity, renal insufficiency, and pregnancy has not been determined. The objective of this review was to assess the safety and efficacy of enoxaparin monitoring via anti-factor Xa levels in high-risk patient populations.
The PubMed database was searched for articles related to low-molecular-weight heparin monitoring. Randomized controlled trials and meta-analyses that evaluated the safety and efficacy of enoxaparin prophylaxis and treatment in patients …
Development, Optimization, And In Vitro/In Vivo Evaluation Of Azelaic Acid Transethosomal Gel For Antidermatophyte Activity, 2023 The British University in Egypt
Development, Optimization, And In Vitro/In Vivo Evaluation Of Azelaic Acid Transethosomal Gel For Antidermatophyte Activity, Ali Nasr, Noha Badawi, Yasmine Tartor, Nader Sobhy, Ali Nasr
Pharmacy
Treatment of dermatophytosis is quite challenging. This work aims to investigate the antidermatophyte action of Azelaic acid (AzA) and evaluate its efficacy upon entrapment into transethosomes (TEs) and incorporation into a gel to enhance its application. Optimization of formulation variables of TEs was carried out after preparation using the thin film hydration technique. The antidermatophyte activity of AzA-TEs was first evaluated in vitro. In addition, two guinea pig infection models with Trichophyton (T.) mentagrophytes and Microsporum (M.) canis were established for the in vivo assessment. The optimized formula showed a mean particle size of 219.8 ± 4.7 nm and a …
Pamam- Cyclodextrin Conjugate Upregulates Brain-Derived Neurotropic Factor In Arpe-19 Cells, 2023 University of South Florida
Pamam- Cyclodextrin Conjugate Upregulates Brain-Derived Neurotropic Factor In Arpe-19 Cells, Gopika Ashokan
USF Tampa Graduate Theses and Dissertations
Background: Traumatic Brain Injury (TBI) is a major contributor to death and disability due to motorvehicle accidents, sports, physical abuse, and battlefield injuries. The primary insult to the brain leads to inflammation, vascular dysfunction, and oxidative stress in the brain as well as in the eye. This leads to loss of Retinal Ganglion Cells (RGCs) and downregulation of Brain derived Neurotopic Factor (BDNF). BDNF is a neurotrophic factor that binds to Tropomyosin Receptor Kinase B (TrkB) receptor to promote cell growth, survival, and differentiation. Current treatment strategies do not promote neuronal regeneration. Therefore, novel treatments are needed to restore vision …
Polyvinylpyrrolidone-Passivated Fluorescent Iron Oxide Quantum Dots For Turn-Off Detection Of Tetracycline In Biological Fluids, 2023 Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C.
Polyvinylpyrrolidone-Passivated Fluorescent Iron Oxide Quantum Dots For Turn-Off Detection Of Tetracycline In Biological Fluids, Sri Sudewi, Lutfi Chabib, Muhammad Zulfajri, Gangaraju Gedda, Genin Gary Huang
Journal of Food and Drug Analysis
Tetracycline is an antibiotic that has been prescribed for COVID-19 treatment, raising concerns about antibiotic resistance after long-term use. This study reported fluorescent polyvinylpyrrolidone-passivated iron oxide quantum dots (IO QDs) for detecting tetracycline in biological fluids for the first time. The as-prepared IO QDs have an average size of 2.84 nm and exist a good stability under different conditions. The IO QDs’ tetracycline detection performance could be attributed to a combination of static quenching and inner filter effect. The IO QDs displayed high sensitivity and selectivity toward tetracycline and achieved a good linear relationship with the corresponding detection limit being …
Chemical Transformation Of Cannabidiol Into Psychotropic Cannabinoids Under Acidic Reaction Conditions: Identification Of Transformed Products By Gc-Ms, 2023 College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea
Chemical Transformation Of Cannabidiol Into Psychotropic Cannabinoids Under Acidic Reaction Conditions: Identification Of Transformed Products By Gc-Ms, Minsun Jeong, Sangin Lee, Chaeyoung Seo, Eunjeong Kwon, Soohyang Rho, Mansoo Cho, Moon Yeon Kim, Wonwoong Lee, Yong Sup Lee, Jongki Hong
Journal of Food and Drug Analysis
Recently, cannabidiol (CBD), one of the major components of the Cannabis species, has been a focus in the cannabis industry due to its various pharmacological effects. Interestingly, CBD can be converted into several psychoactive cannabinoids, such as 9-tetrahydrocannabinol (Δ9-THC) and its structural isomers, under acidic reaction conditions. In this study, chemical transformation of CBD in ethanol solution was conducted with variation in pH at 2.0, 3.5, and 5.0 by addition of 0.1 M hydrochloric acid (HCl). These resulting solutions were derivatized with trimethylsilyl (TMS) reagent and analyzed using GC/MS-scan mode. Time profiles of CBD degradation and transformation of …
Development Of A Metabolomics-Based Data Analysis Approach For Identifying Drug Metabolites Based On High-Resolution Mass Spectrometry, 2023 Department of Anesthesiology, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chia-Yi City 60002, Taiwan, R.O.C.
Development Of A Metabolomics-Based Data Analysis Approach For Identifying Drug Metabolites Based On High-Resolution Mass Spectrometry, Hsiao-Hsien Ting, Yi-Shiou Chiou, Tien-Yi Chang, Guan-Yu Lin, Pei-Jhen Li, Chia-Lung Shih
Journal of Food and Drug Analysis
A metabolomics-based approach to data analysis is required for drug metabolites to be identified quickly. This study developed such an approach based on high-resolution mass spectrometry. Our approach is a two-stage one that combines a time-course experiment with stable isotope tracing. Pioglitazone (PIO) was used to improve glycemic management for type 2 diabetes mellitus. Consequently, PIO was taken as a model drug for identifying metabolites. During Stage I of data analysis, 704 out of 26626 ions exhibited a positive relationship between ion abundance ratio and incubation time in a time-course experiment. During Stage II, 25 isotope pairs were identified among …
Untargeted Metabolomics Analysis Assisted By Signal Selection For Comprehensively Identifying Metabolites Of New Psychoactive Substances: 4-Meo-Α-Pvp As An Example, 2023 Instrumentation Center, National Taiwan University, Taipei 10617, Taiwan, R.O.C.
Untargeted Metabolomics Analysis Assisted By Signal Selection For Comprehensively Identifying Metabolites Of New Psychoactive Substances: 4-Meo-Α-Pvp As An Example, Hsin-Yi Wu, Yuan-Chih Chen, Jing-Fang Hsu, Hsiang-Ting Lu, Yu-Yi Pan, Mi-Chia Ma, Pao-Chi Liao
Journal of Food and Drug Analysis
New psychoactive substances (NPS) have been rapidly emerged as legal alternatives to controlled drugs, which raised severe public health issue. The detection and monitoring of its intake by complete metabolic profiling is an urgent and vital task. Untargeted metabolomics approach has been applied for several NPS metabolites studies. Although the number of such works is relatively limited but with a rapidly growing need. The present study aimed to propose a procedure that includes liquid chromatography high-resolution mass spectrometry (LC-HRMS) analysis and a signal selection software, MetaboFinder, programed as a web tool. The comprehensive metabolites profile of one kind of NPS, …
Guilu Erxian Jiao Enhances Protein Synthesis, Glucose Homeostasis, Mitochondrial Biogenesis And Slow-Twitch Fibers In The Skeletal Muscle, 2023 Department of Pharmacology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C.
Guilu Erxian Jiao Enhances Protein Synthesis, Glucose Homeostasis, Mitochondrial Biogenesis And Slow-Twitch Fibers In The Skeletal Muscle, Wei-Yu Fang, Wan-Hsuan Chang, Yi-Hong Tsai, Hung-Te Hsu, Fang-Rong Chang, Chih-Lung Lin, Yi-Ching Lo
Journal of Food and Drug Analysis
Guilu Erxian Jiao (GEJ) is a commonly used nutritional supplement due to its rich content of amino acids. It is also a traditional herbal medicine for improving degenerative joint. This study aimed to investigate the effect and mechanism of GEJ water extract (GEJ-WE) on skeletal muscle in C2C12 myotubes and C57BL/6J mice. Analysis of GEJ-WE were performed by high-performance liquid chromatography fingerprinting with chemical standards. Protein expression, mRNA level, glycogen content, mitochondria activity and ATP level were evaluated by western blots, real-time PCR, PAS staining, MTT and ATP bioluminescence assay, respectively. Skeletal muscle strength was evaluated by grip strength. Skeletal …
High-Resolution 1h Nmr Analysis Of Continuous And Discontinuous Thermo-Oxidative Susceptibility Of Culinary Oils During Frying At 180℃, 2023 Department of Chemistry and Pharmaceutical Sciences, SEC Faculty, Kingston University, Kingston-upon-Thames, Surrey, KT1 2EE, UK
High-Resolution 1h Nmr Analysis Of Continuous And Discontinuous Thermo-Oxidative Susceptibility Of Culinary Oils During Frying At 180℃, Gilbert Ampem, Adam Le Gresley, Martin Grootveld, Declan P Naughton
Journal of Food and Drug Analysis
Lipid oxidations products (LOPs) are reactive mutagenic and carcinogenic species known to be generated in thermally stressed culinary oils. Mapping the evolution of LOPs in culinary oils exposed to standard frying practices – both continuous and discontinuous thermo-oxidation – at 180℃ is vital to our understanding of these processes, and to the development of scientific solutions for their effective suppression. Modifications in the chemical compositions of the thermo-oxidised oils were analysed using a high-resolution proton nuclear magnetic resonance (1H NMR) technique. Research findings acquired showed that polyunsaturated fatty acid (PUFA)-rich culinary oils were the most susceptible to thermo-oxidation. …
Investigation Of Monacolin K, Yellow Pigments, And Citrinin Production Capabilities Of Monascus Purpureus And Monascus Ruber (Monascus Pilosus), 2023 Department of Life Science, National Taitung University, Taiwan, R.O.C.
Investigation Of Monacolin K, Yellow Pigments, And Citrinin Production Capabilities Of Monascus Purpureus And Monascus Ruber (Monascus Pilosus), Tzu-Shing Lin, Shih-Hau Chiu, Chien-Chi Chen, Chih-Hui Lin
Journal of Food and Drug Analysis
Red mold rice (RMR) is a traditional Chinese medicine prepared using Monascus fermentation. M. ruber (pilosus) and M. purpureus have a long history of use as food and medicine. As an economically important starter culture, the relationship between the taxonomy of Monascus and production capabilities of secondary metabolites is crucial for the Monascus food industry. In this study, monacolin K, monascin, ankaflavin, and citrinin production by M. purpureus and M. ruber were genomically and chemically investigated. Our findings suggest that M. purpureus can produce monascin and ankaflavin in a correlated manner, whereas M. ruber produces monascin with minimum …
Determination Of 24 Sulfonamide Antibiotics In Instant Pastries By Modified Quechers Coupled With Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry, 2023 Shijiazhuang Technology Innovation Center for Chemical Poison Detection and Risk Early Warning, Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, China
Determination Of 24 Sulfonamide Antibiotics In Instant Pastries By Modified Quechers Coupled With Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry, Shuo Li, Can Zhang, Hui-Xin Tang, Yue Gu, Ai-Jing Guo, Ke Wang, Kao-Qi Lian
Journal of Food and Drug Analysis
There were few reports about antibiotic residues in egg-containing products. In the study, an effective method for the simultaneous determination of 24 sulfonamide antibiotics in two instant pastries based on a modified QuEChERS sample preparation technique coupled with ultra performance liquid chromatography-tandem mass spectrometry was developed. The results show that the average recoveries of the SAs at 5, 10, and 50 µg·kg-1 levels were 67.6%-103.8%, with relative standard deviations (RSD) of 0.80-9.23%. The limit of detections (LODs) and limit of quantitations (LOQs) were 0.01-0.14 µg∙kg-1 and 0.02-0.45 µg∙kg-1, respectively. This method was suitable for analysis of …
Erinacine S From Hericium Erinaceus Mycelium Promotes Neuronal Regeneration By Inducing Neurosteroids Accumulation, 2023 Institute of Bioinformatics and Systems Biology, National Yang Ming Chiao Tung University, Taiwan, R.O.C.
Erinacine S From Hericium Erinaceus Mycelium Promotes Neuronal Regeneration By Inducing Neurosteroids Accumulation, Chun-Yu Lin, Yi-Ju Chen, Chih-Hsuan Hsu, Yu-Hsuan Lin, Peng-Tzu Chen, Ting-Han Kuo, Chris T. Ho, Hsin-Hua Chen, Sih-Jia Huang, Ho-Chieh Chiu, Chin-Chu Chen, Eric Hwang
Journal of Food and Drug Analysis
Erinacines derived from Hericium erinaceus have been shown to possess various health benefits including neuroprotective effect against neurodegenerative diseases, yet the underlying mechanism remains unknown. Here we found that erinacine S enhances neurite outgrowth in a cell autonomous fashion. It promotes post-injury axon regeneration of PNS neurons and enhances regeneration on inhibitory substrates of CNS neurons. Using RNA-seq and bioinformatic analyses, erinacine S was found to cause the accumulation of neurosteroids in neurons. ELISA and neurosteroidogenesis inhibitor assays were performed to validate this effect. This research uncovers a previously unknown effect of erinacine S on raising the level of neurosteroids.
Regulatory Considerations For Generic Products Of Non-Biological Complex Drugs, 2023 School of Pharmacy, College of Medicine, National Taiwan University, Taipei City, Taiwan, R.O.C.
Regulatory Considerations For Generic Products Of Non-Biological Complex Drugs, Yu-Hsuan Liu, Yi-Shuo Chen, Ting Tseng, Min-Lin Jiang, Churn-Shiouh Gau, Lin-Chau Chang
Journal of Food and Drug Analysis
The Non-Biological Complex Drug (NBCD) Working Group defines an NBCD as “a medicinal product, not being a biological medicine, where the active substance is not a homo-molecular structure, but consists of different (closely related and often nanoparticulate) structures that cannot be isolated and fully quantitated, characterized and/or described by physicochemical analytical means”. There are concerns about the potential clinical differences between the follow-on versions and the originator products and within the individual follow-on versions. In the present study, we compare the regulatory requirements for developing generic products of NBCDs in the European Union (EU) and the United States (US). The …
Recent Developments In Detection And Therapeutic Approaches For Antibiotic-Resistant Bacterial Infections, 2023 Department of Chemistry, National Dong Hwa University, Taiwan, R.O.C.
Recent Developments In Detection And Therapeutic Approaches For Antibiotic-Resistant Bacterial Infections, Kavya Moorthy, Kai-Chih Chang, Hsueh-Hui Yang, Wen-Min Su, Cheng-Kang Chiang, Zhiqin Yuan
Journal of Food and Drug Analysis
Owing to the widespread emergence and proliferation of antibiotic-resistant bacteria, the therapeutic benefits of antibiotics have been reduced. In addition, the ongoing evolution of multidrug-resistant pathogens poses a challenge for the scientific community to develop sensitive analytical methods and innovative antimicrobial agents for the detection and treatment of drug-resistant bacterial infections. In this review, we have described the antibiotic resistance mechanisms that occur in bacteria and summarized the recent developments in detection strategies for monitoring drug resistance using different diagnostic methods in three aspects, including electrostatic attraction, chemical reaction, and probe-free analysis. Additionally, to understand the effective inhibition of drug-resistant …
Prevention Of Doxorubicin-Induced Cardiotoxicity By A Coumestan Analogue, Psoralidin, 2023 Noorda College of Osteopathic Medicine
Prevention Of Doxorubicin-Induced Cardiotoxicity By A Coumestan Analogue, Psoralidin, Mujtaba H. Shah, Myia Aiges, Kota V. Ramana
Annual Research Symposium
Natural phenolic compound Psoralidin limits cardiotoxicity associated with Doxorubicin
Development Of A Method For Identifying And Quantifying Epicatechin In Cinnamon Extract Supplement Capsules, 2023 Roseman University of Health Sciences
Development Of A Method For Identifying And Quantifying Epicatechin In Cinnamon Extract Supplement Capsules, Danielle Valls
Annual Research Symposium
No abstract provided.
Development Of An Lcms Method To Detect And Quantify Curcumin In A Novel Oral Formulation Of Turmeric, 2023 Roseman University of Health Sciences
Development Of An Lcms Method To Detect And Quantify Curcumin In A Novel Oral Formulation Of Turmeric, Brandon Renninger
Annual Research Symposium
No abstract provided.
A Review On The Impact Of Cannabis In Society And The Analytical Methodologies For Cannabinoids, 2023 Lipscomb University
A Review On The Impact Of Cannabis In Society And The Analytical Methodologies For Cannabinoids, Matt J. Vergne, Lindsey K. Reynolds, Alexus Brown, Grayson Pullias, Anna Froemming
Faculty Works
The use of plant-based medicine dates back centuries, and cannabis (Cannabis sativa) is one such plant that has been used medicinally and illicitly. Although cannabis contains hundreds of cannabinoids and other natural products, its potential medicinal use was largely ignored by modern researchers due to the legal restrictions and heavy regulations introduced in the 1930s. As restrictions on cannabis access have eased since the 1990s there is renewed interest in the research of cannabinoids and the other components in the cannabis plant. The focus of this review article is an overview of cannabis and the analytical challenges in the quality …
Leveraging Bio-Inspired Molecules For Cancer Theranostics, 2023 The Graduate Center, City University of New York
Leveraging Bio-Inspired Molecules For Cancer Theranostics, Douglas S. Macpherson
Dissertations, Theses, and Capstone Projects
A variety of molecules can be radiolabeled and delivered to a cancer site for the purposes of diagnostics and therapy. Among the most promising of tumor targeting molecules are peptides and antibodies. These bio-inspired molecules can be designed and synthesized to target and respond to cancer cells based on the properties of those cells. Matrix metalloproteinase (MMP) enzymes are over-expressed by some metastatic cancers, in which they are responsible for the degradation and remodeling of the extracellular matrix. In recent years, MMPs have emerged as promising targets for enzyme-responsive diagnostic probes because oligopeptides can be designed to be selectively hydrolyzed …
Phase 1a Clinical Study For Q-Griffithsin Intranasal Spray For Prevention Of Coronavirus, 2023 University of Louisville
Phase 1a Clinical Study For Q-Griffithsin Intranasal Spray For Prevention Of Coronavirus, Megan Bezold, Kenneth Palmer, Nobuyuki Matoba, Elizabeth Cash
Posters-at-the-Capitol
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible virus that causes acute respiratory disease with possible long-term complications, known as coronavirus disease 2019 (COVID-19). Griffithsin (GRFT; including the oxidation-resistant variant Q-GRFT used in this study) is an algal carbohydrate-binding protein exhibiting antiviral effects against many enveloped viruses, including all major variants of SARS- CoV-2 reported to date. Q-GRFT has been recombinantly manufactured in Nicotiana benthamiana plants, thoroughly purified, and formulated as an intranasal spray designed to be developed as a non-vaccine broad-spectrum prophylactic product for acute use. This is the first-in-human, randomized, double-blind, placebo-controlled study. The …