Environmental Health Commons^™

Investigating The Toxicity And Accumulation Of 2,4-Dichlorophenoxyacetic Acid (2,4-D) And Glyphosate In Eisenia Fetida, Caitlin Lazurick, Nicole Lidzbarski, Rachel Owings, Jeff Brotherton, Edna Steele

Journal of the South Carolina Academy of Science

Glyphosate, the main ingredient in Roundup^®, is the most widely used herbicide in the world. It is also used in combination with another effective herbicide, 2,4-D, in the formulation called Enlist Duo^®. The EPA approved the use of Enlist Duo^® on certain crops including those genetically modified to be resistant to both herbicides. The predicted significant increase in the use of these herbicides raised concerns from the general public because both compounds have been classified as possibly or probably carcinogenic. Since herbicides are applied directly to vegetation, the safety of organisms that come in contact …

Imidacloprid, A Neonicotinoid Insecticide, Impairs Lipid And Glucose Metabolism, Quancai Sun

Doctoral Dissertations

Emerging evidence suggests that environmental pollutants, including insecticides, are linked to excessive weight gain and altered glucose homeostasis. However, there is currently limited knowledge regarding the biochemical mechanisms by which exposure to insecticides contribute to obesity and its associated pathologies. We first studied the influence of fipronil (a phenylpyrazole family insecticide) on 3T3-L1 adipogenesis. Recently imidacloprid, a neonicotinoid insecticide, was reported to potentiate adipogenesis and insulin resistance in vitro. The current studies also determined the effects of imidacloprid exposure and its interaction with dietary fat in the development of adiposity and insulin resistance using both male and female C57BL/6J …

Robust Biosensors For Healthcare Applications: From High-Content Screening To Point-Of-Care Testing, Ngoc D. B. Le

Doctoral Dissertations

Efficient detection of proteins, mammalian cells, microorganisms and other biological systems in complex mixture is essential in disease diagnosis and environmental health. Therefore, technological platforms that provide sensors of high sensitivity, selectivity and stability are greatly desired. Recently, the ‘chemical-nose’ sensing approach has proved to be an effective strategy for profiling bio-relevant targets in complex mixtures. Detecting analytes in complex mixture is a challenge that conventional specificity-based sensors are still trying to solve due to the requirement of prior knowledge of the analyte, which is unknown in many cases. This thesis focuses on how to develop simple and robust …

Exopolysaccharides Regulate Calcium Flow In Cariogenic Biofilms, Monika Astasov-Frauenhoffer, Muth M. Varenganayil, Alan W. Decho, Tuomas Waltimo, Olivier Braissant

Faculty Publications

Caries-associated biofilms induce loss of calcium from tooth surfaces in the presence of dietary carbohydrates. Exopolysaccharides (EPS) provide a matrix scaffold and an abundance of primary binding sites within biofilms. The role of EPS in binding calcium in cariogenic biofilms is only partially understood. Thus, the aim of the present study is to investigate the relationship between the calcium dissolution rates and calcium tolerance of caries-associated bacteria and yeast as well as to examine the properties of EPS to quantify its binding affinity for dissolved calcium. Calcium dissolution was measured by dissolution zones on Pikovskaya’s agar. Calcium tolerance was assessed …

Bottom-Up Control Of Parasites, David S. Johnson, Richard Heard

VIMS Articles

Parasitism is a fundamental ecological interaction. Yet we understand relatively little about the ecological role of parasites compared to the role of free-living organisms. Bottom-up theory predicts that resource enhancement will increase the abundance and biomass of free-living organisms. Similarly, parasite abundance and biomass should increase in an ecosystem with resource enhancement. We tested this hypothesis in a landscape-level experiment in which salt marshes (60,000 m2 each) received elevated nutrient concentrations via flooding tidal waters for 11 yr to mimic eutrophication. Nutrient enrichment elevated the densities of the talitrid amphipod, Orchestia grillus, and the density and biomass of its …

Ecological Succession On Neotropical Landslides: Comparing Successional Patterns Of Four Different Aged Landslides In The Cerro Candelaria Reserve, Nace Keifer

Independent Study Project (ISP) Collection

Abstract In an area that is vulnerable to frequent landslides due to steep montane slopes and heavy rain falls it is important to understand how the ecosystem regenerates after it has been disturbed. This study examined ecological succession patterns by comparing the vegetation on four different aged landslides and the unharmed forest within the Cerro Candelaria Reserve, located in a cloud forest ecosystem in central Ecuador. One meter soil profiles and 5x5 meter vegetation quadrats were used to observe and characterize the habitats on the four individual landslides and unharmed forest. At each location, soil color, texture, and structure were …

An Ethnobotanical Examination Of Traditional Medicine In Ngezi Forest Reserve, Tyler Tsang

Independent Study Project (ISP) Collection

Traditional medicine is an important aspect of the both the culture and health of communities worldwide. Ngezi Forest Reserve is a protected area on Pemba Island which is part of the Zanzibar Archipelago. This forest contains a wealth of botanical diversity which includes many species of medicinal plants. Traditional healers (waganga) use these medicinal plants to heal members of the community. Interviews and forest walks with these healers were supplemented by consultations with a botanist to determine medicinal value of the forest and the surrounding areas. In compiling information from 15 healers in the area, 98 species of medicinal plants …

Philosophy Bakes No Bread, Babette Babich

Articles and Chapters in Academic Book Collections

Philosophy Bakes No Bread

Far from baking bread, far from practical applicability, philosophy traditionally sought to explain the world, ideally so. Thus, when Marx argued that it was high time philosophy “change the world,” his was a revolutionary challenge. Today, philosophy is an analytic affair and analytic philosophers seek less to explain the world than to squirrel out arguments or, more descriptively, to resolve the minutiae of this or that name problem. Faced with diminishing student demand, analytic philosophers have taken to urging that everyone from primary school students to scientists be required to study (analytic) philosophy. Just so, applied …

Prevalence Of Moniliophthora Roreri In Theobroma Cacao In Relation To Clone Variety, Community Composition, Environmental Factors In An Organic Cacao Farm In Charagre, Bocas Del Toro, Panamá: A Case Study, Kara Eckberg

Independent Study Project (ISP) Collection

Moniliophthora roreri, or Frosty Pod Rot (FPR) is a widespread pathogen that affects the fruit of Theobroma cacao, a tree commonly known as the cacao tree. Often, cultivators seek to control spread of M. roreri through fungicidal compounds. However, these fungicides place selective pressure for resistance on M. roreri. Additionally, they can have adverse environmental and human health effects and are difficult to integrate into practice for smallholders. Therefore organic farms in Bocas del Toro in western Panama, have turned to biocontrol agents to reduce the spread of M. roreri. These strategies include increasing hybrid variety, modifying community composition, altering …

Hydrology And Macroinvertebrates As Biological Indicators- An Integrated Study Of The Río Zamora Watershed, Loja, Ecuador, Isabel Ryde

Independent Study Project (ISP) Collection

Water quality and access is under grave threat in Ecuador, yet remains poorly studied. Macroinvertebrates were used as biological indicators to study water quality around Reserva Natural Madrigal del Podocarpus, Loja Province, Ecuador. Data on macroinvertebrates was combined with hydrologic measurements of stream functions. Macroinvertebrate sampling was conducted from 6 sites within the Upper Río Zamora watershed. Geologic outcrops were observed along several roadcuts and stream banks. Hydrologic data was collected by measuring discharge, elevation change, and finding the highest permanent spring. The EPT index (number of EPT individuals and percentage of EPT individuals) and the BMWP/Colombia Index for macroinvertebrates …

Carbon Sequestration In The Cloud Forest: A Comparative Evaluation Of Aboveground Biomass Carbon Stock Potential In The Río Guajalito Reserve, Elli Mapstone

Independent Study Project (ISP) Collection

As carbon dioxide levels in the atmosphere continue to rise at a rapid rate, it is necessary to understand how forests can both contribute to CO₂ levels but also stop them from rising. Carbon sequestration levels in tropical montane cloud forests are a relatively understudied topic. Gathering carbon stock levels is the first step necessary to start a REDD+ project. Carbon stock levels can be studied on a global, regional or local level. This study used the University of Oxford/ Global Ecosystems Monitoring Network (GEM) methodology to examine carbon sequestration levels of aboveground biomass, specifically ground litter, large branches …

Coral Species Distribution And Percent Cover Of Sessile Organisms On Protected And Non-Protected Coral Reefs In Digir, Buga, Dubbir, And Kanir Kinnidup, Guna Yala, Panama, Sarah Paulson

Independent Study Project (ISP) Collection

Coral reefs are the most productive, biodiverse ecosystems in the ocean despite covering only <.5% of the ocean floor. In today’s changing climate, coral reefs face a multitude of threats including ocean warming, coral bleaching, ocean acidification, overfishing, increasing human population, and coral mining, among others. One way to protect coral reefs is to establish a marine protected area to limit damage and contamination. This study aimed to examine coral species distribution as well as percent cover of sessile organisms and substrates including coral, fire coral, algae, and sponges on protected and non-protected reefs in order to attempt to analyze the effect of protection on coral reefs in Guna Yala, Panama. Based on the data collected, protected areas had higher algae cover (P<.01, df=107), lower coral cover (P<.0001, df=138), and a higher relative abundance of corals with a “weedy” life history such as Porites astreoides (P<.05, df=80). The data also showed that uninhabited islands tended to have significantly higher average coral cover (P<.0001, df=146) and lower average algae cover (P<.0001, df=161), as well as lower relative abundance of P. astreoides (P<.01, df=134). When the protected site sampled was compared to an unprotected site that was around the same island, the protected area was found to have higher coral cover (P<.01, df=71) and lower algae cover (P<.05, df=72) than the unprotected area. Ultimately, lack of human population and protected status were found to be positive factors for coral reef health, and future study should be done to further examine the relationship between these two factors.

Prey Naïveté And Predator-Prey Interactions Between Pterois Volitans, Pomacentridae, And Tetraodontidae In Two Reefs In The Guna Yala Archipelago, Panama, Jesse Garrett-Larsen

Independent Study Project (ISP) Collection

The invasion of Pterois volitans along the Atlantic coast of the Americas is one of the top marine conservation issues of the century. Prey naïveté has been suggested as a mechanism for the success of P. volitans, but conclusive field observations have not been completed. This study tested for prey naïveté in situ by comparing time spent by Pomacentridae and Tetraodontidae in close proximity to P. volitans and a native predator, Cephalopholis cruentata. Difference in time spent by Pomacentridae and Tetraodontidae in the presence of the two predators was observed. The predators P. volitans and C. cruentata were sequentially observed …

Hmgb1-Rage Pathway Drives Peroxynitrite Signaling-Induced Ibd-Like Inflammation In Murine Nonalcoholic Fatty Liver Disease, Varun Chandrashekaran, Ratanesh K. Seth, Diptadip Dattaroy, Firas Alhasson, Jacek Ziolenka, James Carson, Franklin G. Berger, Balaraman Kalyanaraman, Anna Mae Diehl, Saurabh Chatterjee

Faculty Publications

Recent clinical studies found a strong association of colonic inflammation and Inflammatory bowel disease (IBD)-like phenotype with NonAlcoholic Fatty liver Disease (NAFLD) yet the mechanisms remain unknown. The present study identifies high mobility group box 1 (HMGB1) as a key mediator of intestinal inflammation in NAFLD and outlines a detailed redox signaling mechanism for such a pathway. NAFLD mice showed liver damage and release of elevated HMGB1 in systemic circulation and increased intestinal tyrosine nitration that was dependent on NADPH oxidase. Intestines from NAFLD mice showed higher Toll like receptor 4 (TLR4) activation and proinflammatory cytokine release, an outcome strongly …

The Handpump Choice Is Yours: A Pilot Study In Rumphi District, Malawi, Rochelle Holm, Alinafe Kamangira, Victor Kasulo, Prince Kaponda, Edwin Hara, Channing Carney-Filmore, Muthi Nhlema

Faculty Scholarship

In sub-Saharan Africa, moving towards the Sustainable Development Goals will require an approach to water and sanitation service delivery for many rural communities where handpumps still dominate infrastructure. This paper reviews a case study of allowing users (local government and communities) in Rumphi District, Malawi, to choose a handpump model based on information about the life-cycle costs. The results indicate that there is some awareness within communities and within the local government of several handpump options for the rural water supply in the study area. Given a choice of different handpump models in the treatment communities, each community chose the …

Randomized Trial Of Interventions To Improve Childhood Asthma In Homes With Wood-Burning Stoves, Curtis W. Noonan, Erin O. Semmens, Paul Smith, Solomon W. Harrar, Luke Montrose, Emily Weiler, Marcy Mcnamara, Tony J. Ward

Statistics Faculty Publications

BACKGROUND: Household air pollution due to biomass combustion for residential heating adversely affects vulnerable populations. Randomized controlled trials to improve indoor air quality in homes of children with asthma are limited, and no such studies have been conducted in homes using wood for heating.

OBJECTIVES: Our aims were to test the hypothesis that household-level interventions, specifically improved-technology wood-burning appliances or air-filtration devices, would improve health measures, in particular Pediatric Asthma Quality of Life Questionnaire (PAQLQ) scores, relative to placebo, among children living with asthma in homes with wood-burning stoves.

METHODS: A three-arm placebo-controlled randomized trial was conducted in homes with …

Trends In The Environmental Health Job Market For New Graduates, Jason W. Marion, Timothy J. Murphy Phd, Anne Marie Zimeri Phd

EKU Faculty and Staff Scholarship

The question of whether the job market can support future graduates of environmental health programs remains an important and difficult question for environmental health programs, current and prospective students, parents, and other stakeholders. Our previous report using 2014 data from the U.S. Bureau of Labor Statistics demonstrated anticipated growth and higher than average pay in the profession through at least 2022 for baccalaureate degree holders (Marion & Sinde, 2015). Growth in the profession does not necessarily translate into job availability if the market is saturated with job candidates. While university programs produce graduates, local health departments (LHDs) have suffered tremendous …

Lyme Disease And Youtube™: A Cross-Sectional Study Of Video Contents, Corey H. Basch, Lindsay A. Mullican, Kwanza D. Boone, Jingjing Yin, Alyssa Berdnik, Marina E. Eremeeva, Isaac Chun-Hai Fung

Environmental Health Sciences Faculty Publications

Objectives: Lyme disease is the most common tick-borne disease. People seek health information on Lyme disease from YouTubeTM videos. In this study, we investigated if the contents of Lyme disease-related YouTubeTM videos varied by their sources.

Methods: Most viewed English YouTubeTM videos (n = 100) were identified and manually coded for contents and sources.

Results: Within the sample, 40 videos were consumer-generated, 31 were internet-based news, 16 were professional, and 13 were TV news. Compared with consumer-generated videos, TV news videos were more likely to mention celebrities (odds ratio [OR], 10.57; 95% confidence interval [CI], 2.13–52.58), prevention of Lyme disease …

Full-Water Column Turbulence Parameterization Of Stratified Waters In Southern Lake Michigan, Kyla A. Prendergast, Cary D. Troy, David Cannon

The Summer Undergraduate Research Fellowship (SURF) Symposium

Full water column mean flow and turbulence structure was characterized at two stratified locations in Lake Michigan (a. Muskegon, MI; b. Michigan City, IN) in order to better understand the filtration potential of invasive quagga mussels. Invasive quagga mussels in Lake Michigan are filter feeders and can dramatically alter clarity as well as the biological/chemical characteristics of the water column. This filtering capacity is highly contingent on turbulence characteristics throughout the water column, which is poorly understood in the Great Lakes. Using velocity, temperature, and turbulence data collected from these locations, the structure of the water column turbulence was modeled …

County-Level Cumulative Environmental Quality Associated With Cancer Incidence, Jyotsna S. Jagai, Lynne C. Messer, Kristen M. Rappazzo, Christine L. Gray, Shannon C. Grabich, Danelle T. Lobdell

Institute for Sustainable Solutions Publications and Presentations

BACKGROUND: Individual environmental exposures are associated with cancer development; however, environmental exposures occur simultaneously. The Environmental Quality Index (EQI) is a county-level measure of cumulative environmental exposures that occur in 5 domains.

METHODS: The EQI was linked to county-level annual age-adjusted cancer incidence rates from the Surveillance, Epidemiology, and End Results (SEER) Program state cancer profiles. All-site cancer and the top 3 site-specific cancers for male and female subjects were considered. Incident rate differences (IRDs; annual rate difference per 100,000 persons) and 95% confidence intervals (CIs) were estimated using fixed-slope, random intercept multilevel linear regression models. Associations were assessed with …