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Response Of Deer To Containment By A Poly-Mesh Fence For Mitigating Disease Outbreaks, Michael J. Lavelle, Justin W. Fischer, Scott E. Hygnstrom, Joshua J. White, Aaron M. Hildreth, Gregory E. Phillips, Kurt C. Vercauteren 2010 USDA/APHIS/WS National Wildlife Research Center

Response Of Deer To Containment By A Poly-Mesh Fence For Mitigating Disease Outbreaks, Michael J. Lavelle, Justin W. Fischer, Scott E. Hygnstrom, Joshua J. White, Aaron M. Hildreth, Gregory E. Phillips, Kurt C. Vercauteren

USDA National Wildlife Research Center - Staff Publications

Rapidly deployable and effective methods are needed to contain free-ranging deer (Odocoileus spp.) during acute disease outbreaks. We evaluated efficacy of a 2.1-m-tall polypropylene mesh (poly-mesh) fence for containing ≥15 free-ranging white-tailed deer (O. virginianus) within a 42-ha area in eastern Nebraska, USA. We observed a 99% decrease in deer leaving the enclosure area after we installed fencing (1 deer jumped; 0.02 deer/hr) compared with prefence rates (5.26 deer/hr). However, 8 deer (53% of censused population) escaped the enclosure during a census drive after our study. Poly-mesh fencing may be effective in temporarily containing free-ranging ...


Exclusion Fencing For Feral Hogs At White-Tailed Deer Feeders, Justin M. Rattan, Billy J. Higginbotham, David B. Long, Tyler A. Campbell 2010 Texas A&M University-Kingsville

Exclusion Fencing For Feral Hogs At White-Tailed Deer Feeders, Justin M. Rattan, Billy J. Higginbotham, David B. Long, Tyler A. Campbell

USDA National Wildlife Research Center - Staff Publications

Management programs aimed at white-tailed deer (Odocoileus virginianus) production often include the use of feeders, either to deliver supplemental feed or bait. However, much of the feed placed into deer feeders is consumed by non-target species, such as feral hogs (Sus scrofa). Our objectives were to compare three exclusion fence designs at deer feeders for their ability to restrict feral hog visitation and enable white-tailed deer visitation. Our high fence design consisted of 86 cm high graduated paneling. Our medium fence design consisted of 76 cm high, 10´10 cm paneling. Our low height design consisted of 51 cm high ...


Assessment Of Abilities Of White-Tailed Deer To Jump Fences, Kurt C. Vercauteren, Timothy R. Vandeelen, Michael J. Lavelle, Wayne Hall 2010 USDA-APHIS-Wildlife Services

Assessment Of Abilities Of White-Tailed Deer To Jump Fences, Kurt C. Vercauteren, Timothy R. Vandeelen, Michael J. Lavelle, Wayne Hall

USDA National Wildlife Research Center - Staff Publications

There is a need for insight into fence heights required for impeding white-tailed deer (Odocoileus virginianus). We evaluated the ability of wild-caught deer to jump progressively taller fences and documented deterrence rates of 0% for fences ≤1.5 m followed by increasing deterrence rates of 14% at 1.8 m, 85% at 2.1 m, and 100% at 2.4 m. We documented 100% deterrence rates during 5 additional experiments with different deer and the test fence at 2.4 m, a common height of fences at captive deer facilities. Our results will be valuable to those managing spread of ...


Natural History Of Belonuchus Nordmann Spp. And Allies (Coleoptera: Staphylinidae) In Heliconia L. (Zingiberales: Heliconiaceae) Flower Bracts, J. H. Frank, R. Barrera 2010 University of Florida

Natural History Of Belonuchus Nordmann Spp. And Allies (Coleoptera: Staphylinidae) In Heliconia L. (Zingiberales: Heliconiaceae) Flower Bracts, J. H. Frank, R. Barrera

Insecta Mundi

Adults, and in some species larvae, of several members of Belonuchus Nordmann (Coleoptera: Staphylinidae: Staphylininae) and a few related genera seem to be to various degrees consistently associated with flower bracts of the genus Heliconia (Zingiberales: Heliconiaceae). They are predators and eat various dipterous and lepidopterous larvae in that habitat. Adults of at least Belonuchus cephalotes (Sharp) and Odontolinus fasciatus Sharp are able to immerse completely in water to capture larvae and/or pupae of mosquitoes (Culicidae).

Adults and larvae of Belonuchus satyrus Erichson, and adults of B. cacao Blackwelder and B. rufipennis (F.) were found in water-filled flower bracts ...


Afm Study Of Structural Changes In Supported Planar Dppc Bilayers Containing General Anesthetic Isofluorane (Plus Additional Afm Experiments), Chad McKell, Hiram Conley, David D. Busath 2010 Brigham Young University

Afm Study Of Structural Changes In Supported Planar Dppc Bilayers Containing General Anesthetic Isofluorane (Plus Additional Afm Experiments), Chad Mckell, Hiram Conley, David D. Busath

Faculty Publications

Atomic force microscopy (AFM) is a remarkable tool for assessing the structural properties of supported lipid planar bilayers under different physiological conditions. Previous work has shown that incorporation of anesthetics into artificial lipid bilayers results in domain formation [1], destruction of lipid aggregates and patches [2], anesthetic-lipid mixed micelle formation [2], and the development of interdigitated phases of reduced thickness compared to anesthetic-free bilayers [3]. In particular, these interdigitated phases are suspected to affect the structure and activity of membrane proteins, such as ion channels, and thus further research with proteinembedded bilayers exposed to anesthetics could reveal the mechanism responsible ...


Effect Of Ingestion By Alligator Snapping Turtles (Macrochelys Temminckii) On Seeds Of Riparian Vegetation, Jean Pierre Elbers 2010 Missouri State University

Effect Of Ingestion By Alligator Snapping Turtles (Macrochelys Temminckii) On Seeds Of Riparian Vegetation, Jean Pierre Elbers

MSU Graduate Theses

The alligator snapping turtle (Macrochelys temminckii) is a large freshwater turtle that inhabits many lotic water bodies in the Southeastern United States. The species consumes primarily fish but also consumes large amounts of vegetation including seeds of common persimmon (Diospyros virginiana), tupelos (Nyssa sp.), willow oak (Quercus phellos), and pecan (Carya illinoensis). Captive specimens of M. temminckii were fed samples of the above-mentioned seeds to assess how the species affects ingested seeds in order to evaluate the potential role this species may play as a seed disperser. The proportion of seeds defecated intact varied with species (57−99 %), was lowest ...


Relationships Between Jump Characteristics Of Collegiate Female Athletes Competing In Different Disciplines, Christopher J. MacDonalds, Hugh S. Lamont, John C. Garner, Jeremy A. Gentles, Ashley A. Kavanaugh, Michael H. Stone 2010 East Tennessee State University

Relationships Between Jump Characteristics Of Collegiate Female Athletes Competing In Different Disciplines, Christopher J. Macdonalds, Hugh S. Lamont, John C. Garner, Jeremy A. Gentles, Ashley A. Kavanaugh, Michael H. Stone

ETSU Faculty Works

No abstract provided.


Actions Of Ncx, Pmca And Serca On Short-Term Facilitation And Maintenance Of Transmission In Nerve Terminals, Mohati Desai-Shah, Robin L. Cooper 2010 University of Kentucky

Actions Of Ncx, Pmca And Serca On Short-Term Facilitation And Maintenance Of Transmission In Nerve Terminals, Mohati Desai-Shah, Robin L. Cooper

Biology Faculty Publications

Residual Ca2+ can accumulate in the nerve terminal during repetitive stimulation; thus, the basis for short-term facilitation (STF). The plasmalemmal Na+/Ca2+ exchanger [NCX], the Ca2+-ATPase (PMCA) and the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA) on the endoplasmic reticulum are three important Ca2+ regulatory processes in controlling [Ca2+]i. The role of these [Ca2+]i regulators in the development and maintenance of STF was addressed at the neuromuscular junction. When the NCX is compromised by reduced [Na+]o, the EPSP amplitudes decrease, but with KB-R7943 (a reverse blocker of NCX) the amplitude ...


Roles Of The Sarcoplasmic/Endoplasmic Reticulum Ca2+-Atpase, Plasma Membrane Ca2+-Atpase And Na+/Ca2+ Exchanger In Regulation Of Heart Rate In Larval Drosophila, Mohati Desai-Shah, A. R. Papoy, M. Ward, Robin L. Cooper 2010 University of Kentucky

Roles Of The Sarcoplasmic/Endoplasmic Reticulum Ca2+-Atpase, Plasma Membrane Ca2+-Atpase And Na+/Ca2+ Exchanger In Regulation Of Heart Rate In Larval Drosophila, Mohati Desai-Shah, A. R. Papoy, M. Ward, Robin L. Cooper

Biology Faculty Publications

We investigated the roles of three regulatory proteins that impact [Ca2+]i within cardiac myocytes of Drosophila melanogaster. The NCX (Na+/Ca2+ exchanger), PMCA (plasma membrane Ca2+-ATPase) and SERCA (sarcoplasmic/endoplasmic reticulum Ca2+-ATPase) were compromised by ionic, pharmacological, mutationalmanipulation, and with a combination of approaches, while heart rate (HR) was monitored. A decrease in SERCA function reduced HR more for intact larva in comparison to a dissected larva. Dissected preparations were used to expose the heart directly to agents. A compromised PMCA also reduced HR; however, attenuated NCX function by low [Na+]○ increased HR ...


Ethylene Receptors Function As Components Of High-Molecular-Mass Protein Complexes In Arabidopsis, Brad Binder, Yi-Feng Chen, Zhiyong Gao, Robert Kerris, Wuyi Wang, G. Schaller 2009 University of Tennessee - Knoxville

Ethylene Receptors Function As Components Of High-Molecular-Mass Protein Complexes In Arabidopsis, Brad Binder, Yi-Feng Chen, Zhiyong Gao, Robert Kerris, Wuyi Wang, G. Schaller

Brad M. Binder

Background The gaseous plant hormone ethylene is perceived in Arabidopsis thaliana by a five-member receptor family composed of ETR1, ERS1, ETR2, ERS2, and EIN4. Methodology/Principal Findings Gel-filtration analysis of ethylene receptors solubilized from Arabidopsis membranes demonstrates that the receptors exist as components of high-molecular-mass protein complexes. The ERS1 protein complex exhibits an ethylene-induced change in size consistent with ligand-mediated nucleation of protein-protein interactions. Deletion analysis supports the participation of multiple domains from ETR1 in formation of the protein complex, and also demonstrates that targeting to and retention of ETR1 at the endoplasmic reticulum only requires the first 147 amino ...


Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek 2009 The University of Tennessee

Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek

Seung J Baek

Expression of cyclooxygenases (COX) and lipoxygenases (LOX) has been linked to many pathophysiological phenotypes, including cell adhesion. However, many current approaches to measure cellular changes are performed only in a fixed-time point. Since cells dynamically move in conjunction with the cell matrix, there is a pressing need for dynamic or time-dependent methods for the investigation of cell properties. In the presented study, we used stable human colorectal cancer cell lines ectopically expressing COX-1, COX-2, and 15LOX-1, to investigate whether expression of COX-1, COX-2, or 15LOX-1 would affect cell adhesion using our opto-electric methodology. In a fixed-time point experiment, only COX-1- ...


The Tick Saliva Protein, Salp15, Contributes To Th17-Induced Pathology During Experimental Autoimmune Encephalomyelitis, Juan Anguita, I.J. Juncadella, T.C. Bates, R. Suleiman, A. Monteagudo-Mera, C.M. Olson, N. Navasa, E.R. Olivera, C. Teuscher, B.A. Osborne 2009 University of Massachusetts - Amherst

The Tick Saliva Protein, Salp15, Contributes To Th17-Induced Pathology During Experimental Autoimmune Encephalomyelitis, Juan Anguita, I.J. Juncadella, T.C. Bates, R. Suleiman, A. Monteagudo-Mera, C.M. Olson, N. Navasa, E.R. Olivera, C. Teuscher, B.A. Osborne

Juan Anguita

No abstract provided.


Delineation Of Precursors In Murine Spleen That Develop In Contact With Splenic Endothelium To Give Novel Dendritic-Like Cells., Jonathan Tan, Pravin Periasamy, Helen O'Neill 2009 Australian National University

Delineation Of Precursors In Murine Spleen That Develop In Contact With Splenic Endothelium To Give Novel Dendritic-Like Cells., Jonathan Tan, Pravin Periasamy, Helen O'Neill

Helen O'Neill

Hematopoietic cell lineages are best described in terms of distinct progenitors with limited differentiative capacity. To distinguish cell lineages, it is necessary to define progenitors and induce their differentiation in vitro. We previously reported in vitro development of immature dendritic-like cells (DCs) in long-term cultures (LTCs) of murine spleen, and in cocultures of spleen or bone marrow (BM) over splenic endothelial cell lines derived from LTCs. Cells produced are phenotypically distinct CD11b(hi)CD11c(lo)CD8(-)MHC-II(-) cells, tentatively named L-DCs. Here we delineate L-DC progenitors as different from known DC progenitors in BM and DC precursors in spleen. The ...


A Rapid Direct Solvent Extraction Method For The Extraction Of 2-Dodecylcyclobutanone From Irradiated Ground Beef Patties Using Acetonitrile., Amit Kumar 2009 Kansas State University

A Rapid Direct Solvent Extraction Method For The Extraction Of 2-Dodecylcyclobutanone From Irradiated Ground Beef Patties Using Acetonitrile., Amit Kumar

Amit Kumar, DVM, MS, PhD

The amount of irradiated beef in the U.S. market is growing, and a reliable, rapid method is needed to detect irradiated beef and quantify the irradiation dose. The official analytical method (BS EN 1785 2003) that has been adopted by the European Union is time consuming. The objective of this study was to develop a rapid method for the analysis of 2-dodecylcyclobutanone (2-DCB) in irradiated beef. A 5 g sample of commercially irradiated ground beef patty (90/10) was extracted with n-hexane using a Soxhlet apparatus or with acetonitrile via direct solvent extraction. The Soxhlet hexane extract was evaporated ...


Early Cranial Patterning In The Direct-Developing Frog Eleutherodactylus Coqui Revealed Through Gene Expression, Ryan Kerney, Joshua Gross, James Hanken 2009 Gettysburg College

Early Cranial Patterning In The Direct-Developing Frog Eleutherodactylus Coqui Revealed Through Gene Expression, Ryan Kerney, Joshua Gross, James Hanken

Ryan Kerney

Genetic and developmental alterations associated with the evolution of amphibian direct development remain largely unexplored. Specifically, little is known of the underlying expression of skeletal regulatory genes, which may reveal early modifications to cranial ontogeny in direct-developing species. We describe expression patterns of three key skeletal regulators (runx2, sox9, and bmp4) along with the cartilage-dominant collagen 2a1 gene (col2a1) during cranial development in the direct- developing anuran, Eleutherodactylus coqui. Expression patterns of these regulators reveal transient skeletogenic anlagen that correspond to larval cartilages, but which never fully form in E. coqui. Suprarostral anlagen in the frontonasal processes are detected through ...


Bioelectronics, J. Fleming, Steven Ripp 2009 University of Tennessee - Knoxville

Bioelectronics, J. Fleming, Steven Ripp

Steven Ripp

The Encyclopedia of Industrial Biotechnology was published in order to help readers make sense of the vast amounts of information that have been published around the world across a broad array of ournals, books, and websites. With its comprehensive coverage, Encyclopedia of Industrial Biotechnology is the ideal starting point for research projects involving any aspect of industrial biological processes, including fermentation, biocatalysis, bioseparation, and biofabrication


Fitness Variation Due To Sexual Antagonism And Linkage Disequilibrium, Manus Patten, David Haig, Fransisco Úbeda de Torres 2009 University of Tennessee, Knoxville

Fitness Variation Due To Sexual Antagonism And Linkage Disequilibrium, Manus Patten, David Haig, Fransisco Úbeda De Torres

Francisco Úbeda de Torres

Extensive fitness variation for sexually antagonistic characters has been detected in nature. However, current population genetic theory suggests that sexual antagonism is unlikely to play a major role in the maintenance of variation. We present a twolocus model of sexual antagonism that is capable of explaining greater fitness variance at equilibrium than previous single-locus models. The second genetic locus provides additional fitness variance in two complementary ways. First, linked loci can maintain gene variants that are lost in single-locus models of evolution, expanding the opportunity for polymorphism. Second, linkage disequilibrium results between any two sexually antagonistic genes, producing an excess ...


Bacteriophage Probe-Based Biosensors, M. Zourob, Steven Ripp 2009 University of Tennessee - Knoxville

Bacteriophage Probe-Based Biosensors, M. Zourob, Steven Ripp

Steven Ripp

This book presents a significant and up-to-date review of various recognition receptors, their immobilization, and an overview of surface characterization techniques. Distinguished scientists from key institutions worldwide have contributed chapters that provide a deep analysis of their particular subjects; at the same time, each topic is framed within the context of this integrated approach. This work explores comprehensively the field of recognition receptors, presenting a thorough review of the subject and effective integration of these receptors on sensor surfaces in order to appropriately convey state-of the-art fundamentals and applications of the most innovative approaches.


Biosensors As Environmental Monitors, S. Ripp, M. DiClaudio, Gary Sayler 2009 University of Tennessee, Knoxville

Biosensors As Environmental Monitors, S. Ripp, M. Diclaudio, Gary Sayler

Gary S. Sayler

This is the long-awaited and much-anticipated revision of the bestselling text and reference. Based on the latest information and investigative techniques from molecular biology and genetics, this Second Edition offers an in-depth examination of the role of microbiological processes related to environmental deterioration with an emphasis on the detection and control of environmental contaminants. Its goal is to further our understanding of the complex microbial processes underlying environmental degradation, its detection and control, and ultimately, its prevention.


Diagnostic Exercise: Chronic Vomiting In A Dog., Debra Miller 2009 University of Tennessee, Knoxville

Diagnostic Exercise: Chronic Vomiting In A Dog., Debra Miller

Debra L Miller

An approximately one-and-a-half-year-old, neutered male, mixed-breed dog was presented for a chronic history of vomiting. Profuse diarrhea was also noted during examination. An exploratory laparotomy was performed, bone chips were removed from the stomach, and a raised, circular area of gastric mucosa was biopsied. Histologically, there was severe gastric cryptosporidiosis as well as numerous spiral bacteria, consistent with Helicobacter spp. Polymerase chain reaction revealed visible bands for the 18S ribosomal RNA gene for Cryptosporidium spp. The polymerase chain reaction product was sequenced and was found to be most similar to Cryptosporidium muris. Both the gastric location and the species of ...


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