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Inhibitors Of Pyruvate Carboxylase, Tonya N. Zeczycki, Martin St. Maurice, Paul V. Attwood 2010 University of Wisconsin - Madison

Inhibitors Of Pyruvate Carboxylase, Tonya N. Zeczycki, Martin St. Maurice, Paul V. Attwood

Biological Sciences Faculty Research and Publications

This review aims to discuss the varied types of inhibitors of biotin-dependent carboxylases, with an emphasis on the inhibitors of pyruvate carboxylase. Some of these inhibitors are physiologically relevant, in that they provide ways of regulating the cellular activities of the enzymes e.g. aspartate and prohibitin inhibition of pyruvate carboxylase. Most of the inhibitors that will be discussed have been used to probe various aspects of the structure and function of these enzymes. They target particular parts of the structure e.g. avidin – biotin, FTP – ATP binding site, oxamate – pyruvate binding site, phosphonoacetate – binding site of the putative carboxyphosphate ...


The Adoption Of Best Management Practices In The Louisiana Crawfish Industry, Narayan P. Nyaupane 2010 Louisiana State University and Agricultural and Mechanical College

The Adoption Of Best Management Practices In The Louisiana Crawfish Industry, Narayan P. Nyaupane

LSU Master's Theses

Agricultural production can have result in environmental deterioration in cases where proper management practices have not been implemented. Louisiana, one of the tropical states, has a significant agricultural base with more than 1,600 farmers raising crawfish. Large volumes of waste water containing environmental pollutants result significant environmental problem in the state. Voluntary adoption of a number of best management practices (BMPs) that are considered to be environmentally and economically beneficial is encouraged in Louisiana. The major objectives of this study are to investigate farmer adoption of 18 selected Natural Resources Conservation Service (NRCS) cost share eligible BMPs and the ...


New Binary Ti Vectors With Co-Directional Replicons For Agrobacterium Tumefaciens-Mediated Transformation Of Higher Plants, Seokhyun Lee 2010 Louisiana State University and Agricultural and Mechanical College

New Binary Ti Vectors With Co-Directional Replicons For Agrobacterium Tumefaciens-Mediated Transformation Of Higher Plants, Seokhyun Lee

LSU Doctoral Dissertations

Small, high-yielding binary Ti vectors of Agrobacterium tumefaciens were constructed. All four basic components of the vector, ColE1 replicon (715 bp) for Escherichia coli, VS1 replicon (2,659 bp) for A. tumefaciens, a bacterial kanamycin resistance gene (999 bp), and the T-DNA region (170 bp), were modified to reduce the vector size to 4,566 bp and to introduce a number of mutations to increase the copy number and other functionality. The transcriptional direction of VS1 replicon can be the same as that of ColE1 replicon (co-directional transcription), or opposite (head-on transcription) as in the case of widely used vectors ...


Target Eccentricity Effects For Defensive Responses, Taegyong Kwon 2010 Louisiana State University and Agricultural and Mechanical College

Target Eccentricity Effects For Defensive Responses, Taegyong Kwon

LSU Doctoral Dissertations

Defensive actions involving goal-directed responses to visual stimuli presented in different parts of the viewing field commonly include movements either toward (TOWARD) or away from (AWAY) the actual stimulus. One can categorize the type of defensive movements by outcome or the level of stimulus-response (S-R) compatibility, where a congruent response corresponds to a response in the TOWARD condition and an incongruent response corresponds to a response in the AWAY condition. In an effort to better understand defensive responses, which have received less attention in the literature than offensive movements regardless of their importance in combative situations, we studied the responses ...


Carbohydrate Sources And Maximizing The Use Of Supplemental Amino Acids In Diets For Weanling Pigs, Victor D. Naranjo 2010 Louisiana State University and Agricultural and Mechanical College

Carbohydrate Sources And Maximizing The Use Of Supplemental Amino Acids In Diets For Weanling Pigs, Victor D. Naranjo

LSU Doctoral Dissertations

The objectives of this research were 1) to determine the effect of replacing dried whey (DW) with milk chocolate product (MCP), dried whey permeate (DWP) with candy oats (CO), and spray-dried plasma protein (SDPP) with a novel swine nutritional supplement (SNS) on growth performance of weanling pigs, and 2) to determine the maximum level of supplemental L-Lys, along with DL-Met, L-Thr, and L-Trp that can be added in diets for 6- to 12 and 13- to 23-kg pigs. Three experiments were conducted to compare the feeding value of MCP (20% lactose and 60% sugars) and DW (70% lactose). Results from ...


Evaluation Of Green Tea Extract As A Glazing Material For Shrimp Frozen By Cryogenic And Air-Blast Freezing, Srijanani Sundararajan 2010 Louisiana State University and Agricultural and Mechanical College

Evaluation Of Green Tea Extract As A Glazing Material For Shrimp Frozen By Cryogenic And Air-Blast Freezing, Srijanani Sundararajan

LSU Master's Theses

Green tea (Camellia sinensis) extract (GTE) is rich in polyphenolic compounds, especially catechins that are potent antioxidants. The antioxidant property of GTE may make it ideal for use as a glazing solution for suppressing lipid oxidation in shrimp (Litopenaeus setiferus) during frozen storage. In this study, GTE was evaluated as a glazing material for shrimp frozen by air-blast (BF) and cryogenic freezing (CF). Two percent, three percent and/or five percent green tea extract solutions (2GTE, 3GTE, 5TGE) were used for glazing. Distilled water glazed (GDW) and non-glazed (NG) shrimp were used as controls. The GTE was characterized by measuring ...


Application Of Anti-H Monoclonal Antibodies To Develop Rapid Immunoassay For Vibrio Vulnificus, Ravirajsinh Jadeja 2010 Louisiana State University and Agricultural and Mechanical College

Application Of Anti-H Monoclonal Antibodies To Develop Rapid Immunoassay For Vibrio Vulnificus, Ravirajsinh Jadeja

LSU Doctoral Dissertations

In the US there are about 76 million foodborne illness cases reported every year, in spite of initiatives by federal agencies. This emphasizes the need for the development of novel detection techniques which are sensitive, specific and rapid. A detection technique should be rapid enough to give results same day, allowing companies to release food lots without delay and decreasing storage costs. This kind of screening would also allow immediate measures, if needed, before releasing the lots. In the case of Vibrio vulnificus (V. vulnificus), conventional methods are available to identify and enumerate this pathogen in oysters, but they are ...


Effects Of Emulsion Coatings On The Internal Quality And Shelf Life Of Eggs, Damir Dennis Torrico 2010 Louisiana State University and Agricultural and Mechanical College

Effects Of Emulsion Coatings On The Internal Quality And Shelf Life Of Eggs, Damir Dennis Torrico

LSU Master's Theses

Mineral oil (MO) is currently used for coating eggs to preserve quality. Chitosan possesses inherent antimicrobial and film-forming properties. Chitosan coating (CH) is dried much faster than MO when applied on egg shell. Information on synergistic effects of MO:CH emulsion coatings on egg quality does not exist. We developed MO:CH emulsion coatings, and evaluated their effects on internal quality and shelf-life of eggs during storage. In the first study, MO, CH and three emulsions (MO:CH = 75:25, 50:50, and 25:75) were evaluated during 5 weeks at 25°C. Haugh unit (HU) and yolk index values ...


An Economic Analysis Of Cover Crops In Corn-Dominated Production Systems, Sukirti Nepal 2010 Louisiana State University and Agricultural and Mechanical College

An Economic Analysis Of Cover Crops In Corn-Dominated Production Systems, Sukirti Nepal

LSU Master's Theses

A survey data by Singer et al. (2007) was used to study the factors affecting the adoption of cover crops by the producers at the Corn Belt area of the United States. Data was collected from four states: Illinois, Indiana, Iowa, and Minnesota. Two binomial logit models were used for the econometric analysis. The first logit model was used to observe the factors affecting the adoption of cover crops by the producers. The second logit model was used to analyze the factors affecting the adoption of cover crops by producers in recent years, specifically, in past five years. The result ...


Association Of Esterases In Resistance To Naled And Resmethrin In The Southern House Mosquito, Culex Quinquefasciatus, Jennifer R. Gordon 2010 Louisiana State University and Agricultural and Mechanical College

Association Of Esterases In Resistance To Naled And Resmethrin In The Southern House Mosquito, Culex Quinquefasciatus, Jennifer R. Gordon

LSU Master's Theses

The vector-competency and its affinity for humans make C. quinquefasciatus an important target of mosquito abatement programs. Whenever such control programs are implemented, protocols to monitor frequencies and mechanisms of resistance to the insecticides used are necessary to optimize the efficacy of the management strategy and to slow development of resistance to the insecticides used. In the current study, susceptibilities to the two adulticides used by EBRMARC (naled and resmethrin) were monitored using field-collected mosquitoes and a topical and contact bioassay, respectively. My hypothesis was that esterase-mediated enhanced metabolism conferred resistance to both insecticides in populations of C. quinquefasciatus from ...


Physiological, Biochemical And Molecular Characteristics Associated With Virulence Of Burkholderia Glumae: The Major Causative Agent Of Bacterial Panicle Blight Of Rice, Hari Sharan Karki 2010 Louisiana State University and Agricultural and Mechanical College

Physiological, Biochemical And Molecular Characteristics Associated With Virulence Of Burkholderia Glumae: The Major Causative Agent Of Bacterial Panicle Blight Of Rice, Hari Sharan Karki

LSU Master's Theses

Burkholderia glumae is the bacterial pathogen causing bacterial panicle blight disease of rice (BPB). The pathogen, whose growth and pathogenicity is favored by high temperatures, has become a serious threat to rice production around the world possibly due to the current global climate changes. In this study, previously reported avirulent B. glumae strains were characterized in respect to the production of known virulence factors, toxoflavin, lipase and motility, and potential virulence factors, including polygalacturnase and type III secretion system (hypersensitive response). Considerable phenotypic variation was observed among the strains tested. Virulence of the B. glumae strains was closely related to ...


Determination Of The Economic Optimal Cycle Length For Major Sugarcane (Saccharum Spp.) Varieties In Louisiana, Juan Steer Nunes 2010 Louisiana State University and Agricultural and Mechanical College

Determination Of The Economic Optimal Cycle Length For Major Sugarcane (Saccharum Spp.) Varieties In Louisiana, Juan Steer Nunes

LSU Master's Theses

The general objective of the study was to determine the economically optimal crop cycle length for major sugarcane varieties currently being produced in Louisiana. The specific objectives of the project included the specification of the mathematical acreage relationships which directly impact the production of a vegetatively propagated perennial crop in a whole farm context; the development of producer decision rules to be used to determine breakeven sugar levels on third stubble sugarcane crops for major varieties in the state; the evaluation of the impact of changes in production factors on developed crop replacement rules; and the optimal cycle length for ...


Copper Oxide Wire Particles In Feed Pellets For Controlling Gastrointestinal Nematode Infection In Ewes And Lambs, Sarah Tammy Nicole Orlik 2010 Louisiana State University and Agricultural and Mechanical College

Copper Oxide Wire Particles In Feed Pellets For Controlling Gastrointestinal Nematode Infection In Ewes And Lambs, Sarah Tammy Nicole Orlik

LSU Master's Theses

Gastrointestinal nematode parasites cause extensive damage to small ruminants, and Haemonchus contortus is a major concern to production. In the past, small ruminants were dewormed at regular intervals and control methods were based primarily on the use of anthelmintics. At present, anthelmintic resistance has been reported worldwide and has developed into a serious problem for small ruminant management programs. In view of this, alternate control methods are needed. One alternative method is the use of copper oxide wire particles (COWP). Three independent trials were conducted during Spring, Summer, and Fall of 2008. Two trials evaluated the effect of COWP in ...


Three Essays On The Role Of Amenities As An Economic Development Strategy, Junpyo Hong 2010 Louisiana State University and Agricultural and Mechanical College

Three Essays On The Role Of Amenities As An Economic Development Strategy, Junpyo Hong

LSU Doctoral Dissertations

It is well known that an amenity is a key driving engine to regional economic growth. However, the site-specific nature of an amenity can characterize them as public goods. Due to this characteristic, local governments have difficulty optimally supplying amenities. This dissertation tries to find relationships between an amenity and economic growth. Three empirical papers comprise the original research in this dissertation. The findings of the meta-analysis in the first essay suggest little methodological diversity exists among researchers linking amenities to economic growth., I do find that employment growth is more likely related to man-made amenities even in research on ...


Quantifying Tarnished Plant Bug, Lygus Lineolaris (Palisot De Beauvois), Resistance To Acephate In Louisiana, Josh T. Copes 2010 Louisiana State University and Agricultural and Mechanical College

Quantifying Tarnished Plant Bug, Lygus Lineolaris (Palisot De Beauvois), Resistance To Acephate In Louisiana, Josh T. Copes

LSU Master's Theses

The tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), is one of the most yield- limiting insect pests attacking cotton in the Mid-Southern region of the U.S. This pest is almost exclusively managed with chemical control strategies. The organophosphate insecticide, acephate, has been one of the most important insecticides recommended to control tarnished plant bugs. In recent years, reports of unsatisfactory acephate performance have become common and actual field rates have been increased to improve control. The objective of this study was to survey acephate susceptibility in Louisiana populations of tarnished plant bug using laboratory bioassays and evaluate acephate ...


Pregnancy Rates In Beef Cattle Artifically Inseminated With Frozen-Thawed Aged Beef Semen, David Barry Carwell 2010 Louisiana State University and Agricultural and Mechanical College

Pregnancy Rates In Beef Cattle Artifically Inseminated With Frozen-Thawed Aged Beef Semen, David Barry Carwell

LSU Master's Theses

The duration of frozen semen storage had no effect on pregnancy rates (60%, 61% and 61%, respectively) in Angus females inseminated with semen from 25 Angus bulls from Time Frames 1 (1960-1975), 2 (1976-1991) and 3 (1992-2006). Pregnancy rates were not different for beef females inseminated with semen from Time Frames 1, 2 and 3 (55%, 62% and 55%, respectively). There were no differences for total and progressive sperm motility across Time Frames 1 (42% and 29%), Time Frame 2 (51% and 38%) and Time Frame 3 (55% and 41%) and percentage of normal and abnormal sperm from bulls in ...


Formation Of The Neural Tube Epithelium Basement Membrane During Secondary Neurulation In The Chick Embryo, Leslie Ann Mataya 2010 University of Northern Iowa

Formation Of The Neural Tube Epithelium Basement Membrane During Secondary Neurulation In The Chick Embryo, Leslie Ann Mataya

Honors Program Theses

Neurulation, the formation of the neural tube, is an important process in the development of vertebrates. Because this organ ultimately becomes the central nervous system of the organism, the proper development of a fully-functioning neural tube is paramount to its survival. Neural tube defects, like spina bifida and anencephaly, are among the most common congenital malformations present in live human births. The prevalence of these defects is a definite concern to medicine and human health.


Mitochondrial Physiology Of Diapausing And Developing Embryos Of The Annual Killifish Austrofundulus Limnaeus: Implications For Extreme Anoxia Tolerance, Jeffrey M. Duerr, Jason E. Podrabsky 2010 George Fox University

Mitochondrial Physiology Of Diapausing And Developing Embryos Of The Annual Killifish Austrofundulus Limnaeus: Implications For Extreme Anoxia Tolerance, Jeffrey M. Duerr, Jason E. Podrabsky

Biology Faculty Publications and Presentations

Diapausing embryos of the annual killifish Austrofundulus limnaeus have the highest reported anoxia tolerance of any vertebrate and previous studies indicate modified mitochondrial physiology likely supports anoxic metabolism. Functional mitochondria isolated from diapausing and developing embryos of the annual killifish exhibited VO2, respiratory control ratios (RCR), and P:O ratios consistent with those obtained from other ectothermic vertebrate species. Reduced oxygen consumption associated with dormancy in whole animal respiration rates are correlated with maximal respiration rates of mitochondria isolated from diapausing versus developing embryos. P:O ratios for developing embryos were similar to those obtained from adult liver, but were ...


European Starling Preferences For Bait Substrates Used In Drc-1339 Applications, H. Jeffrey Homan, George M. Linz, Scott Beckerman, Anthony G. Duffiney, Thomas D. Halstead 2010 USDA/APHIS/Wildlife Services’ National Wildlife Research Center

European Starling Preferences For Bait Substrates Used In Drc-1339 Applications, H. Jeffrey Homan, George M. Linz, Scott Beckerman, Anthony G. Duffiney, Thomas D. Halstead

Human–Wildlife Interactions

Additional bait substrates for the avicide, DRC-1339 Concentrate (3-chloro-4- methylaniline hydrochloride), could provide USDA/Wildlife Services with more fl exibility when managing nuisance populations of European starlings (Sturnus vulgaris) at livestock facilities. From January 11 to 21, 2008, we conducted 11 2-choice preference tests with 6 bait types at a feedlot in central Kansas. The baits included cracked corn mixed with lard (2 concentrations), 2 forms of distiller’s grain (wet powder and pellets), 2 types of livestock feed (calf-starter pellet and sweet-feed mix), and a custom-produced poultry pellet (carrier pellet) made by USDA specifically for baiting starlings. We evaluated ...


Testing Fladry As A Nonlethal Management Tool For Wolves And Coyotes In Michigan, Sarah J. Davidson-Nelson, Thomas M. Gehring 2010 Central Michigan University

Testing Fladry As A Nonlethal Management Tool For Wolves And Coyotes In Michigan, Sarah J. Davidson-Nelson, Thomas M. Gehring

Human–Wildlife Interactions

Several forms of nonlethal management exist, but fi eld testing is problematic, and few such techniques have been tested on free-ranging wolves (Canis lupus) or other predators. We tested fladry in the eastern Upper Peninsula of Michigan during the summers of 2004 and 2005 on treatment farms and control farms. Wolf visitation inside pastures, compared to those outside pastures, was less on fladry-protected farms (U = 45, n = 7, P = 0.004); whereas, we found no difference in wolf visitation inside and outside of pastures on control farms (U = 30, n = 7, P = 0.24). We found no difference in coyote ...


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