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Laser-Assisted Zona Pellucida Hatching Of Frozen-Thawed In Vivo-Produced Bovine Embryos, Mindy Kaye Chiasson 2010 Louisiana State University and Agricultural and Mechanical College

Laser-Assisted Zona Pellucida Hatching Of Frozen-Thawed In Vivo-Produced Bovine Embryos, Mindy Kaye Chiasson

LSU Master's Theses

Incomplete zona hatching or failure of the zona to rupture compromises post-transfer embryo viability and conceptus development. Assisted hatching prior to the transfer of frozen-thawed bovine embryos has been proposed as a means to increase recipient pregnancy rates. The objective of this study was to determine if laser-assisted hatching would improve in vivo-produced frozen-thawed bovine embryo hatching and pregnancy rates. In Experiment I,II and III frozen direct-transfer embryos received either two a or three symmetrical rents at either 40% or 80% through the outer zona surface using the Hamilton Thorne XYClone® (Hamilton Thorne Biosciences) diode laser at 90% power ...


Incentives, Risk, And The Role Of Private Investments In Louisiana Coastal Wetland Restoration, cheikhna Ould Dedah 2010 Louisiana State University and Agricultural and Mechanical College

Incentives, Risk, And The Role Of Private Investments In Louisiana Coastal Wetland Restoration, Cheikhna Ould Dedah

LSU Doctoral Dissertations

The coast of Louisiana, with more than three million wetland acres, accounts for about 40 percent of the nation’s total wetlands. Louisiana is estimated to have lost more than 1.2 million acres of its coastal wetland in the last century. Although 75% of Louisiana’s coastal wetlands are privately owned, little has been done to encourage private landowners to undertake wetland restoration projects. This dissertation examines the factors that influence the decisions of the landowners to undertake wetland restoration projects. We develop a theoretical framework for understanding the landowner’s decision-making process in the presence of high uncertainty ...


Optimized Extraction Of Soluble Defatted Rice Bran Fiber And Its Application For Microencapsulation Of Fish Oil, Yuting Wan 2010 Louisiana State University and Agricultural and Mechanical College

Optimized Extraction Of Soluble Defatted Rice Bran Fiber And Its Application For Microencapsulation Of Fish Oil, Yuting Wan

LSU Master's Theses

Defatted rice bran (DRB) is a byproduct of rice milling and rice bran oil extraction. Soluble rice bran fiber (SRBF) extracted from defatted rice bran is known for its antioxidant activity and hypocholesterolemic effects in human, while purified menhaden oil (PMO) is a good source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The goal of the study was to estimate optimum extraction conditions to extract SRBF from DRB, develop a cost effective method to purify SRBF and produce microencapsulated PMO with SRBF. The response surface methodology showed that an estimated optimum yield of SRBF (7.89%) could be extracted ...


Environmental Correlates Of Abundances Of Mosquito Species And Stages In Discarded Vehicle Tires, Donald A. Yee, Jamie M. Kneitel, Steven A. Juliano 2010 University of Southern Mississippi

Environmental Correlates Of Abundances Of Mosquito Species And Stages In Discarded Vehicle Tires, Donald A. Yee, Jamie M. Kneitel, Steven A. Juliano

Faculty Publications

Discarded vehicle tires are a common habitat for container mosquito larvae, although the environmental factors that may control their presence or abundance within a tire are largely unknown. We sampled discarded vehicle tires in six sites located within four counties of central Illinois during the spring and summer of 2006 to determine associations between a suite of environmental factors and community composition of container mosquitoes. Our goal was to find patterns of association between environmental factors and abundances of early and late instars. We hypothesized that environmental factors correlated with early instars would be indicative of oviposition cues, whereas environmental ...


Detect Live Salmonella Cells In Produce By Coupling Propidium Monoazide With Loop-Mediated Isothermal Amplification (Pma-Lamp), Siyi Chen 2010 Louisiana State University and Agricultural and Mechanical College

Detect Live Salmonella Cells In Produce By Coupling Propidium Monoazide With Loop-Mediated Isothermal Amplification (Pma-Lamp), Siyi Chen

LSU Master's Theses

Salmonella is a leading cause of foodborne illnesses worldwide. In recent years, an increasing number of Salmonella-related outbreaks in produce has been reported. It is therefore important that the produce industry be equipped with rapid, sensitive, specific detection methods for live Salmonella cells in produce to better ensure the produce safety. In this study, we first designed and optimized a loop-mediated isothermal amplification (LAMP) assay for Salmonella detection by targeting the invasion gene (invA). Then we incorporated a chemical reagent, propidium monoazide (PMA) into the sample preparation step to prevent LAMP amplification of dead Salmonella cells. To our knowledge, this ...


Prepartum Maternal Cortisol Concentrations On Postnatal Cortisol Concentration And Immunoglobulin Absorption In Neonatal Dairy Calves, Dana Nadine Wooley 2010 Louisiana State University and Agricultural and Mechanical College

Prepartum Maternal Cortisol Concentrations On Postnatal Cortisol Concentration And Immunoglobulin Absorption In Neonatal Dairy Calves, Dana Nadine Wooley

LSU Master's Theses

The role of glucocorticoids on intestinal closure in neonates has recently become an area of interest but a definitive mechanism remains to be identified. It is known that glucocorticoids enhance immunoglobulin absorption in dairy calves, but the role of maternal glucocorticoids at parturition is not clear. In the present experiment, we obtained plasma and milk samples from primiparous and multiparous Holstein cows (n=24) to measure cortisol at 72, 48 and 24 hours before and 3, 6, 12, 24 and 48 hours after spontaneous parturition. After parturition, calves (n=24) were immediately removed from their dams and a blood sample ...


Effects Of Emulsion Coatings On The Internal Quality And Shelf Life Of Eggs, Damir Dennis Torrico 2010 Louisiana State University and Agricultural and Mechanical College

Effects Of Emulsion Coatings On The Internal Quality And Shelf Life Of Eggs, Damir Dennis Torrico

LSU Master's Theses

Mineral oil (MO) is currently used for coating eggs to preserve quality. Chitosan possesses inherent antimicrobial and film-forming properties. Chitosan coating (CH) is dried much faster than MO when applied on egg shell. Information on synergistic effects of MO:CH emulsion coatings on egg quality does not exist. We developed MO:CH emulsion coatings, and evaluated their effects on internal quality and shelf-life of eggs during storage. In the first study, MO, CH and three emulsions (MO:CH = 75:25, 50:50, and 25:75) were evaluated during 5 weeks at 25°C. Haugh unit (HU) and yolk index values ...


Cooking Times And Temperatures For Safe Consumption Of Louisiana Blue Crabs (Callinectes Sapidus), Nicole Watson Hazard 2010 Louisiana State University and Agricultural and Mechanical College

Cooking Times And Temperatures For Safe Consumption Of Louisiana Blue Crabs (Callinectes Sapidus), Nicole Watson Hazard

LSU Master's Theses

While all seafood has the potential of being associated with foodborne illness, Louisiana blue crabs (Callinectes sapidus) are exposed to Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Listeria monocytogenes and Salmonella species in the estuarine habitat. This study was designed to determine the least amount of time and temperature needed to reduce or eliminate each of the aforementioned bacteria from a single Louisiana blue crab with either boiling or steaming heat treatments. Once the single crab heat treatment studies were completed, the bacteria that showed the greatest thermal resistance, Listeria monocytogenes, and the bacteria most associated with foodborne illness in Louisiana ...


Individuality In Gut Microbiota Composition Is A Complex Polygenic Trait Shaped By Multiple Environmental And Host Genetic Factors, Andrew K. Benson, Scott A. Kelly, Ryan Legge, Fangrui Ma, Soo Jen Low, Jaehyoung Kim, Min Zhang, Phaik Lyn Oh, Derrick Nehrenberg, Kunjie Hua, Stephen D. Kachman, Etsuko N. Moriyama, Jens Walter, Daniel A. Peterson, Daniel Pomp 2010 University of Nebraska-Lincoln

Individuality In Gut Microbiota Composition Is A Complex Polygenic Trait Shaped By Multiple Environmental And Host Genetic Factors, Andrew K. Benson, Scott A. Kelly, Ryan Legge, Fangrui Ma, Soo Jen Low, Jaehyoung Kim, Min Zhang, Phaik Lyn Oh, Derrick Nehrenberg, Kunjie Hua, Stephen D. Kachman, Etsuko N. Moriyama, Jens Walter, Daniel A. Peterson, Daniel Pomp

Faculty Publications in Food Science and Technology

In vertebrates, including humans, individuals harbor gut microbial communities whose species composition and relative proportions of dominant microbial groups are tremendously varied. Although external and stochastic factors clearly contribute to the individuality of the microbiota, the fundamental principles dictating how environmental factors and host genetic factors combine to shape this complex ecosystem are largely unknown and require systematic study. Here we examined factors that affect microbiota composition in a large (n = 645) mouse advanced intercross line originating from a cross between C57BL/6J and an ICR-derived outbred line (HR). Quantitative pyrosequencing of the microbiota defined a core measurable microbiota (CMM ...


Weed Genomics Advance: A Commentary, Stephen O. Duke, Jonathan Gressel 2010 USDA

Weed Genomics Advance: A Commentary, Stephen O. Duke, Jonathan Gressel

Publications from USDA-ARS / UNL Faculty

A recent book edited by Neal Stewart summarized the salient information about the genomics of weeds. It is a relatively slim volume, in part because so little is known of weed genomics. The rapidly evolving weeds of modern agriculture cry out for understanding their evolution at the genomic level. Still, granting agencies have been reluctant to fund weed genomics in the past, stating that it was enough to support the sequencing of economically important plants (crops), and forgetting that there may be use to sequencing plants that have extremely negative economicimpactsonthose crops. This attitude hasbeenchanging, and the costs have come ...


Prey Preference As A Function Of Feeding Experience And Prey Type In Neonate Gartersnakes (Colubridae: Thamnophis), Kathryn B. Hale 2010 Eastern Illinois University

Prey Preference As A Function Of Feeding Experience And Prey Type In Neonate Gartersnakes (Colubridae: Thamnophis), Kathryn B. Hale

Masters Theses

No abstract provided.


Estimation Of Effective Plant Area Index For South Korean Forests Using Lidar System, D. A. Kwak, W. K. Lee, Menas Kafatos, Y. Son, H. K. Cho, S. H. Lee 2010 Korea University

Estimation Of Effective Plant Area Index For South Korean Forests Using Lidar System, D. A. Kwak, W. K. Lee, Menas Kafatos, Y. Son, H. K. Cho, S. H. Lee

Mathematics, Physics, and Computer Science Faculty Articles and Research

Light Detection and Ranging (LiDAR) systems can be used to estimate both vertical and horizontal forest structure. Woody components, the leaves of trees and the understory can be described with high precision, using geo-registered 3D-points. Based on this concept, the Effective Plant Area Indices (PAIe) for areas of Korean Pine (Pinus koraiensis), Japanese Larch (Larix leptolepis) and Oak (Quercus spp.) were estimated by calculating the ratio of intercepted and incident LIDAR laser rays for the canopies of the three forest types. Initially, the canopy gap fraction (GLiDAR) was generated by extracting the LiDAR data reflected from the canopy surface, or ...


Leptodactylus Savagei, W. R. Heyer, Miriam M. Heyer, Rafael O. de Sá 2010 University of Richmond

Leptodactylus Savagei, W. R. Heyer, Miriam M. Heyer, Rafael O. De Sá

Biology Faculty Publications

Adult Leptodactylus savagei are large, the head is as wide as long or usually wider than long, and the hind limbs are moderate in length (Table 1; Heyer and Thompson (2000) provided definitions of adult size and leg length categories for Leptodactylus). Male vocal sacs are not visible externally. Sexually active males have hypertrophied forearms, usually 1 large black spine on each thumb, rarely with 1 large spine and a prepollical bump, and a pair of black chest spines. A pair of entire dorsolateral folds extend anteriorly from at least one_half to full distance from eye to groin, the dorsolateral ...


2010 Wildflower Of The Year: Wild Ginger, Asarum Canadense, W. John Hayden 2010 University of Richmond

2010 Wildflower Of The Year: Wild Ginger, Asarum Canadense, W. John Hayden

Biology Faculty Publications

Wild ginger is a low herbaceous plant. The stem consists of a branched creeping rhizome at or just below the soil surface. Soft-hairy leaves arise in pairs annually from rhizome branches. Petioles can be up to 20 cm long, elevating the 7—25 mm wide kidney-shaped leaf blades above the forest floor. Small flowers appear in the spring shortly after the leaves have expanded. Typically, one must push the leaves aside in order to glimpse the jug-like flowers. A single flower stalk appears between the paired leaf bases, but it is short and barely lifts the flower above the soil ...


Don't Judge A Book By Its Cover: The Curious Case Of Wild Ginger Pollination, W. John Hayden 2010 University of Richmond

Don't Judge A Book By Its Cover: The Curious Case Of Wild Ginger Pollination, W. John Hayden

Biology Faculty Publications

What pollinates wild ginger? This seems like an easy question. The inconspicuous little flowers are held close to the forest floor, often completely hidden by a dense canopy of ginger leaves above. Flower color is rather drab, dominated by brown and maroon hues. Wind pollination seems completely unlikely and flowers pollinated by bees, butterflies, moths, or hummingbirds are always much more showy and accessible to these flying creatures. Flies, however, given their natural inclination to seek carrion as a food source for their babies (i.e. maggots), are often attracted to brown and maroon flowers. And because their actual quarry ...


Characterization Of Tram, A Required Bacteroides Fragilis Conjugal Apparatus Protein That Interacts With The B. Fragilis Putative Coupling Protein Bcta And The B. Fragilis Relaxase Protein Bmph, Mai Thi Thanh Nguyen 2010 Loyola University Chicago

Characterization Of Tram, A Required Bacteroides Fragilis Conjugal Apparatus Protein That Interacts With The B. Fragilis Putative Coupling Protein Bcta And The B. Fragilis Relaxase Protein Bmph, Mai Thi Thanh Nguyen

Dissertations

Bacteroides spp. organisms, the most prominent bacteria of the human colon, are reservoirs of antibiotic resistance genes which promiscuously transfer within and from the genus via conjugation. The B. fragilis clinical isolate LV23 harbors a 37kb conjugative transposon, BTF-37. Two important conjugal apparatus proteins encoded by BTF-37 are BctA, a predicted coupling protein (CP) that powers DNA translocation, and ORF7 (TraM). In this study, we demonstrated that TraM exhibits characteristics of a required conjugal apparatus protein including 1) upregulated expression under conjugation conditions; 2) localization to the bacterial inner membrane; 3) interaction with the putative coupling protein BctA and the ...


Hindbrain Serotonin Neurons Activate 5-Ht1a Receptors In The Nucleus Tractus Solitarius (Nts) To Modulate Sympathetic And Ventilatory Recovery Following Hypotensive Hemorrhage, Ling-Hsuan Kung 2010 Loyola University Chicago

Hindbrain Serotonin Neurons Activate 5-Ht1a Receptors In The Nucleus Tractus Solitarius (Nts) To Modulate Sympathetic And Ventilatory Recovery Following Hypotensive Hemorrhage, Ling-Hsuan Kung

Dissertations

Trauma is the leading cause of death of young people in the United States. Most such deaths result either from the initial blood loss or from secondary reperfusion injury following resuscitation.

Currently, the standard treatments for hypovolemic shock include volume resuscitation and using of vasoconstrictors to raise pressure. However, these treatments can exacerbate reperfusion injury, and require technical care when apply to patients. Therefore, novel therapies that can safely restore perfusion pressure are needed.

Our lab has found that 5-HT1A-receptor agonist, 8-OH-DPAT, increases perfusion pressure and improves acid-base balance in hypovolemic rats by stimulation of sympathetic-mediated increases in venous tone ...


Technical Note: Hydraulic Property Determination Of Denitrifying Bioreactor Fill Media, Laura E. Christianson, Adrià Castelló, Reid D. Christianson, Matthew J. Helmers, Alok Bhandari 2010 Iowa State University

Technical Note: Hydraulic Property Determination Of Denitrifying Bioreactor Fill Media, Laura E. Christianson, Adrià Castelló, Reid D. Christianson, Matthew J. Helmers, Alok Bhandari

Agricultural and Biosystems Engineering Publications

Denitrification bioreactors are one of the newest options for nitrate removal in agricultural drainage waters. Optimization of denitrification bioreactor design requires the ability to identify concrete values for the hydraulic properties of bioreactor fill media. Hydraulic properties, chiefly saturated hydraulic conductivity but also porosity and particle size, are not known for many types of possible bioreactor media though they have a significant impact upon bioreactor design and performance. This work was undertaken to more fully quantify the hydraulic properties of the major type of fill media used in Iowa denitrification bioreactors through a series of porosity, hydraulic conductivity, and particle ...


Effect Of Ultrasonic Pretreatment On Methane Production Potential From Corn Ethanol Coproducts, Wei Wu-Haan, Robert T. Burns, Lara B. Moody, Cody John Hearn, David A. Grewell 2010 Iowa State University

Effect Of Ultrasonic Pretreatment On Methane Production Potential From Corn Ethanol Coproducts, Wei Wu-Haan, Robert T. Burns, Lara B. Moody, Cody John Hearn, David A. Grewell

Agricultural and Biosystems Engineering Publications

This article addresses the biochemical methane potential (BMP) production from anaerobic digestion of corn-ethanol coproducts including dried distiller grain with solubles (DDGS), distiller's wet grains (DWG), thin stillage, and condensed distiller's solubles (CDS) as well as evaluating the effects of ultrasonic pretreatment on methane production from these feedstocks. Ultrasonic pretreatment was applied with three amplitude settings of 33% (52.8 µmpp), 66% (105.6 µmpp), and 100% (160 µmpp) as well as five time settings (10, 20, 30, 40, and 50 s) to each of the four coproducts prior to conducting benchtop BMP trials. Ultrasonic pretreatment reduced mean ...


Prediction Of Indoor Climate And Long-Term Air Quality Using The Bta-Aqp Model: Part I. Bta Model Development And Evaluation, Gang Sun, Steven J. Hoff 2010 Iowa State University

Prediction Of Indoor Climate And Long-Term Air Quality Using The Bta-Aqp Model: Part I. Bta Model Development And Evaluation, Gang Sun, Steven J. Hoff

Agricultural and Biosystems Engineering Publications

The objective of this research was to develop a building thermal analysis and air quality predictive (BTA-AQP) model to predict ventilation rate, indoor temperature, and long-term air quality (NH3, H2S, and CO2 concentrations and emissions) for swine deep-pit buildings. This article, part I of II, presents a lumped capacitance model (BTA model) to predict the transient behavior of ventilation rate and indoor air temperature according to the thermo-physical properties of a typical swine building, setpoint temperature scheme, fan staging scheme, transient outside temperature, and the heat fluxes from pigs and supplemental heaters. The obtained ventilation rate and resulting indoor air ...


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