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The Evaluation Of Conservation Practice Placement In The Little River Experimental Watershed Using Geographic Information Systems, J Settimi, Dana Sullivan, Timothy Strickland 2009 Eastern Kentucky University

The Evaluation Of Conservation Practice Placement In The Little River Experimental Watershed Using Geographic Information Systems, J Settimi, Dana Sullivan, Timothy Strickland

John R. Settimi

The Conservation Effects Assessment Program Watershed Assessment Study is a joint effort between the USDA Natural Resources Conservation Service (NRCS) and the USDA Agricultural Research Service to evaluate the effectiveness of federally funded conservation programs. In response to this initiative, a 26-year history of NRCS conservation practice placement (1980 to 2006) was evaluated for the Little River Experimental Watershed (LREW) in the southeastern coastal plain of Georgia. To accomplish this task, currently available geographic databases were integrated and queried to assess levels of commonly adopted practices and to evaluate factors affecting practice placement. Databases included (1) USDA NRCS Conservation Practice ...


Effects Of 12- And 24-Week Multimodal Interventions On Physical Activity, Nutritional Behaviors, And Body Mass Index And Its Psychological Predictors In Severely Obese Adolescents At Risk For Diabetes, Jim Annesi, Ann M. Walsh, Alice E. Smith 2009 Kennesaw State University

Effects Of 12- And 24-Week Multimodal Interventions On Physical Activity, Nutritional Behaviors, And Body Mass Index And Its Psychological Predictors In Severely Obese Adolescents At Risk For Diabetes, Jim Annesi, Ann M. Walsh, Alice E. Smith

Jim Annesi

Background: Although 7% of US adolescents have impaired fasting glucose, a precursor of type 2 diabetes, research has suggested that few interventions for obese adolescents at risk for diabetes have been effective. Therefore, pediatricians seek effective behavioral treatments for referral for this age group.

Objective: We wanted to determine the effects of two different durations of nutritional and exercise treatments on changes in nutrition, physical activity, body mass index (BMI), and psychological predictors of BMI change in overweight and obese adolescents at risk for type 2 diabetes.

Methods: We obtained data from 64 pediatrician-referred patients with diabetes risk factors (mean ...


Relations Of Changes In Self-Regulatory Efficacy And Physical Self-Concept With Improvements In Body Satisfaction In Obese Women Initiating Exercise With Cognitive-Behavioral Support, Jim Annesi 2009 Kennesaw State University

Relations Of Changes In Self-Regulatory Efficacy And Physical Self-Concept With Improvements In Body Satisfaction In Obese Women Initiating Exercise With Cognitive-Behavioral Support, Jim Annesi

Jim Annesi

Effects of exercise on body image are not well understood. Obese women seeking weight loss were randomly assigned to groups of exercise supported by a cognitive-behavioral protocol (n = 68) or typical introductory methods (n = 66), and assessed on body satisfaction (Body Areas Satisfaction Scale; BASS), self-regulatory efficacy (Exercise Self-Efficacy Scale; ESE), physical self-concept (Physical Self-Concept; PSC), weight, and body composition, over 6 months. Improvements on all measures were greatest in the cognitive-behavioral support condition. Improvement in BASS scores was better predicted by changes in the two psychological measures (PSC, ESE; R2 adj ¼ :30 and :32, respectively) than changes in ...


Dc Expressing Transgene Foxp3 Are Regulatory Apc, Michael Lipscomb 2009 Howard University

Dc Expressing Transgene Foxp3 Are Regulatory Apc, Michael Lipscomb

Michael Lipscomb

Tolerogenic DC and suppressive Foxp3(+) Treg play important roles in preventing autoimmunity and allograft rejection. We report that (adenovirus mediated) ectopic expression of Foxp3 in human DC (i.e. DC.Foxp3) yields an APC that severely limits T-cell proliferation and type-1 immune responses from the naïve, but not memory, pool of responder T cells in vitro. In marked contrast, the frequencies of type-2 and Treg responses were dramatically increased after stimulation of naïve T cells with DC.Foxp3 versus control DC. DC.Foxp3-induced CD4(+)CD25(+) Treg cells potently suppressed the proliferation of, and IFN-gamma production from, CD4(+) and CD8(+) responder ...


Novel Thiochromane Ring Modified Sheta2 Analogs Inhibit Growth Of Er Positive Breast Cancer Cell Mcf7 [Abstract], Shengquan Liu, Maggie Louie 2009 Touro University

Novel Thiochromane Ring Modified Sheta2 Analogs Inhibit Growth Of Er Positive Breast Cancer Cell Mcf7 [Abstract], Shengquan Liu, Maggie Louie

Maggie Louie

No abstract available


Lifestyle, Anthropometric, And Obesity-Related Physiologic Determinants Of Insulin-Like Growth Factor-1 In The Third National Health And Nutrition Examination Survey (1988-1994).Pdf, Niyati Parekh, Calpurnyia B. Roberts, Maya Vadiveloo, Thanusha Puvananayagam, Jeanine Albu, Grace L. Lu-Yao 2009 University of Rhode Island

Lifestyle, Anthropometric, And Obesity-Related Physiologic Determinants Of Insulin-Like Growth Factor-1 In The Third National Health And Nutrition Examination Survey (1988-1994).Pdf, Niyati Parekh, Calpurnyia B. Roberts, Maya Vadiveloo, Thanusha Puvananayagam, Jeanine Albu, Grace L. Lu-Yao

Maya Vadiveloo

PURPOSE: Epidemiologic studies suggest that insulin-like growth factor-1 (IGF-1) is associated with obesity and, more recently, cancer. This study investigates multiple lifestyle, physiologic, and anthropometric determinants of circulating IGF-1 concentrations.
METHODS: Nationally representative data were used from the cross-sectional Third National Health and Nutrition Examination (NHANES III, 1988–1994) survey, which measured IGF-1 concentrations in blood, from a subsample of participants who were examined in the morning. After exclusion of persons with missing data, 6,058 men and women 20 years of age or older were included in the study.
RESULTS: The mean IGF-1 concentrations were 260 ng/mL in ...


The Intellectual Property Landscape For Ips Cells, Robin C. Feldman 2009 University of California, Hastings

The Intellectual Property Landscape For Ips Cells, Robin C. Feldman

Robin C Feldman

Beginning in 2006, induced pluripotent stem cells have raised the tantalizing possibility that stem cell research could move forward without the significant moral and ethical dilemmas that have paralyzed the field. These cells, known as iPS cells, originate from adult somatic cells, but function in a manner that is almost equivalent to embryonic stem cells. If iPS cell research lives up to its promise, stem cell research, diagnostics, and treatment could be accomplished without destroying or in any way interfering with human embryos or their development.

While we may be entering a historic moment in stem cell research, we are ...


Nomenclatural Changes For Some Grasses In California And The Muhlenbergia Clade (Poaceae), James Payne Smith Jr. 2009 Humboldt State University

Nomenclatural Changes For Some Grasses In California And The Muhlenbergia Clade (Poaceae), James Payne Smith Jr.

James Payne Smith

No abstract provided.


Contributions Of Francisella Tularensis Subsp. Novicida Chitinases And Sec Secretion System To Biofilm Formation On Chitin, Jeffrey J. Margolis, Sahar H. El-Etr, Lydia-Marie Joubert, Emily Moore, Richard Robinson, Amy Rasley, Alfred M. Spormann, Denise M. Monack 2009 Stanford University School of Medicine

Contributions Of Francisella Tularensis Subsp. Novicida Chitinases And Sec Secretion System To Biofilm Formation On Chitin, Jeffrey J. Margolis, Sahar H. El-Etr, Lydia-Marie Joubert, Emily Moore, Richard Robinson, Amy Rasley, Alfred M. Spormann, Denise M. Monack

Jeffrey Margolis

Francisella tularensis, the zoonotic cause of tularemia, can infect numerous mammals and other eukaryotes.
Although studying F. tularensis pathogenesis is essential to comprehending disease, mammalian infection is
just one step in the ecology of Francisella species. F. tularensis has been isolated from aquatic environments and
arthropod vectors, environments in which chitin could serve as a potential carbon source and as a surface for
attachment and growth. We show that F. tularensis subsp. novicida forms biofilms during the colonization of
chitin surfaces. The ability of F. tularensis to persist using chitin as a sole carbon source is dependent on
chitinases, since ...


Autonomous Bioluminescent Expression Of The Bacterial Luciferase Gene Cassette (Lux) In A Mammalian Cell Line, Dan M. Close, Stacey S. Patterson, Steven Ripp, Seung J. Baek, John Sanseverino, Gary S. Sayler 2009 University of Tennessee - Knoxville

Autonomous Bioluminescent Expression Of The Bacterial Luciferase Gene Cassette (Lux) In A Mammalian Cell Line, Dan M. Close, Stacey S. Patterson, Steven Ripp, Seung J. Baek, John Sanseverino, Gary S. Sayler

Steven Ripp

Background

The bacterial luciferase (lux) gene cassette consists of five genes (luxCDABE) whose protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations. Historically expressible only in prokaryotes, the lux operon was re-synthesized through a process of multi-bicistronic, codon-optimization to demonstrate for the first time self-directed bioluminescence emission in a mammalian HEK293 cell line in vitro and in vivo.

Methodology/Principal Findings

Autonomous in vitro light production was shown to be 12-fold greater than the observable background associated with untransfected control cells. The availability of reduced riboflavin phosphate (FMNH2) was identified as the limiting bioluminescence ...


Spatial Structure And Activity Of Sedimentary Microbial Communities Underlying A Beggiatoa Spp. Mat In A Gulf Of Mexico Hydrocarbon Seep, Karen Lloyd, Daniel B. Albert, Jennifer F. Biddle, Jeffrey P. Chanton, Oscar Pizarro, Andreas Teske 2009 Selected Works

Spatial Structure And Activity Of Sedimentary Microbial Communities Underlying A Beggiatoa Spp. Mat In A Gulf Of Mexico Hydrocarbon Seep, Karen Lloyd, Daniel B. Albert, Jennifer F. Biddle, Jeffrey P. Chanton, Oscar Pizarro, Andreas Teske

Karen Lloyd

Background Subsurface fluids from deep-sea hydrocarbon seeps undergo methane- and sulfur-cycling microbial transformations near the sediment surface. Hydrocarbon seep habitats are naturally patchy, with a mosaic of active seep sediments and non-seep sediments. Microbial community shifts and changing activity patterns on small spatial scales from seep to non-seep sediment remain to be examined in a comprehensive habitat study. Methodology/Principal Findings We conducted a transect of biogeochemical measurements and gene expression related to methane- and sulfur-cycling at different sediment depths across a broad Beggiatoa spp. mat at Mississippi Canyon 118 (MC118) in the Gulf of Mexico. High process rates within ...


Ganaxolone Suppression Of Behavioral And Electrographic Seizures In The Mouse Amygdala Kindling Model, Doodipala S. Reddy, Michael A. Rogawski 2009 University of California - Davis

Ganaxolone Suppression Of Behavioral And Electrographic Seizures In The Mouse Amygdala Kindling Model, Doodipala S. Reddy, Michael A. Rogawski

Michael A. Rogawski

Ganaxolone (3alpha-hydroxy-3alpha-methyl-5alpha-pregnan-20-one), a synthetic analog of the endogenous neurosteroid allopregnanolone and a positive allosteric modulator of GABA-A receptors, may represent a new treatment approach for epilepsy. Here we demonstrate that pretreatment with ganaxolone (1.25—20 mg/kg, s.c.) causes a dose-dependent suppression of behavioral and electrographic seizures in fully amygdala-kindled female mice, with nearly complete seizure protection at the highest dose tested. The ED50 for suppression of behavioral seizures was 6.6 mg/kg. The seizure suppression produced by ganaxolone was comparable to that of clonazepam (ED50, 0.1 mg/kg, s.c.). To the extent that amygdala ...


Indigenous Ectosymbiotic Bacteria Associated With Diverse Hydrothermal Vent Invertebrates, Shana K. Goffredi 2009 Occidental College

Indigenous Ectosymbiotic Bacteria Associated With Diverse Hydrothermal Vent Invertebrates, Shana K. Goffredi

Shana Goffredi

Symbioses involving bacteria and invertebrates contribute to the biological diversity and high productivity of both aquatic and terrestrial environments. Well-known examples from chemosynthetic deep-sea hydrothermal vent environments involve ectosymbiotic microbes associated with the external surfaces of marine invertebrates. Some of these ectosymbioses confer protection or defence from predators or the environment itself, some are nutritional in nature, and many still are of unknown function. Several recently discovered hydrothermal vent invertebrates, including two populations of yeti crab (Kiwa spp.), a limpet (Symmetromphalus aff. hageni), and the scaly-foot snail (as yet undescribed), support a consortium of diverse bacteria. Comparisons of these ectosymbioses ...


A New Genus To Accommodate Gymnopus Acervatus (Agaricales), Karen Hughes, David A. Mather, Ronald H. Peterson 2009 University of Tennessee, Knoxville

A New Genus To Accommodate Gymnopus Acervatus (Agaricales), Karen Hughes, David A. Mather, Ronald H. Peterson

Karen Hughes

Phylogenies based on ITS and LSU nrDNA sequences show Agaricus (Gymnopus) acervatus as unique within the Gymnopus/Rhodocollybia complex. These phylogenies imply that a separate genus is necessary, and Connopus is proposed. Infraspecific morphological and DNA-based variation within C. acervatus suggests that a western North American clade might be reproductively isolated from the eastern North American/Scandinavian clade and that in this species complex the European and eastern North American clade might be conspecific. A Scandinavian exemplar is selected for bar-coding. Two GenBank sequences with name-phylogenetic placement inconsistencies are identified.


The 1.9 Å Structure Of Human Α-N-Acetylgalactosaminidase The Molecular Basis Of Schindler And Kanzaki Diseases, Nathaniel E. Clark, Scott Garman 2009 University of Massachusetts - Amherst

The 1.9 Å Structure Of Human Α-N-Acetylgalactosaminidase The Molecular Basis Of Schindler And Kanzaki Diseases, Nathaniel E. Clark, Scott Garman

Scott Garman

alpha-N-acetylgalactosaminidase (alpha-NAGAL; E.C. 3.2.1.49) is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalactosamine residues from glycopeptides and glycolipids. In humans, a deficiency of alpha-NAGAL activity results in the lysosomal storage disorders Schindler disease and Kanzaki disease. To better understand the molecular defects in the diseases, we determined the crystal structure of human alpha-NAGAL after expressing wild-type and glycosylation-deficient glycoproteins in recombinant insect cell expression systems. We measured the enzymatic parameters of our purified wild-type and mutant enzymes, establishing their enzymatic equivalence. To investigate the binding specificity and catalytic mechanism of the human alpha-NAGAL enzyme, we determined three ...


Autonomous Bioluminescent Expression Of The Bacterial Luciferase Gene Cassette (Lux) In A Mammalian Cell Line, Dan M. Close, Stacey S. Patterson, Steven Ripp, Seung J. Baek, John Sanseverino, Gary S. Sayler 2009 University of Tennessee - Knoxville

Autonomous Bioluminescent Expression Of The Bacterial Luciferase Gene Cassette (Lux) In A Mammalian Cell Line, Dan M. Close, Stacey S. Patterson, Steven Ripp, Seung J. Baek, John Sanseverino, Gary S. Sayler

Gary S. Sayler

Background

The bacterial luciferase (lux) gene cassette consists of five genes (luxCDABE) whose protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations. Historically expressible only in prokaryotes, the lux operon was re-synthesized through a process of multi-bicistronic, codon-optimization to demonstrate for the first time self-directed bioluminescence emission in a mammalian HEK293 cell line in vitro and in vivo.

Methodology/Principal Findings

Autonomous in vitro light production was shown to be 12-fold greater than the observable background associated with untransfected control cells. The availability of reduced riboflavin phosphate (FMNH2) was identified as the limiting bioluminescence ...


Dispersal Of Stable Flies (Diptera: Muscidae) From Larval Developmental Sites., David B. Taylor, Roger D. Moon, John B. Campbell, Dennis R. Berkebile, Philip J. Scholl, Alberto B. Broce, Jerome A. Hogsette 2009 University of Minnesota - Twin Cities

Dispersal Of Stable Flies (Diptera: Muscidae) From Larval Developmental Sites., David B. Taylor, Roger D. Moon, John B. Campbell, Dennis R. Berkebile, Philip J. Scholl, Alberto B. Broce, Jerome A. Hogsette

David B. Taylor

Seven mark-recapture studies were conducted over 3 yr to assess dispersal of newly emerging adult stable flies, Stomoxys calcitrans L., from larval development sites in a mixed agricultural environment in northeastern Nebraska. Infested hay debris piles were marked by dusting their surfaces with fluorescent pigments, adults were captured with surrounding grids of Alsynite sticky traps, and specimens were dissected to determine feeding histories and reproductive age. Distances and directions of 3,889 marked specimens indicated males and females dispersed equally and in all directions. Midguts of males and females were equally likely to contain blood-meal remnants. Percentage with blood remnants ...


Criterion Iv: Social And Economic Indicators Of Rangeland Sustainability, Mark W. Brunson, Daniel W. McCollum, Louis E. Swanson, John A. Tanaka, Aaron J. Harp, L. Allen Torell, H. Theodore Heintz Jr. 2009 Utah State University

Criterion Iv: Social And Economic Indicators Of Rangeland Sustainability, Mark W. Brunson, Daniel W. Mccollum, Louis E. Swanson, John A. Tanaka, Aaron J. Harp, L. Allen Torell, H. Theodore Heintz Jr.

John A Tanaka

Social and economic systems provide the context and rationale for rangeland management. Sustaining rangeland ecosystems requires attention to the social and economic conditions that accompany the functioning of those systems. We present and discuss economic and social indicators for rangeland sustainability. A brief conceptual basis for each indicator is offered, describing its potential relationship to rangelands.


The Construction And Analysis Of Marker Gene Libraries, S.M. Short, F. Chen, Steven Wilhelm 2009 University of Tennessee, Knoxville

The Construction And Analysis Of Marker Gene Libraries, S.M. Short, F. Chen, Steven Wilhelm

Steven Wilhelm

Marker genes for viruses are typically amplified from aquatic samples to determine whether specific viruses are present in the sample, or to examine the diversity of a group of related viruses. In this chapter, we will provide an overview of common methods used to amplify, clone, sequence, and analyze virus marker genes, and will focus our discussion on viruses infecting algae, bacteria, and heterotrophic flagellates. Within this chapter, we endeavor to highlight critical aspects and components of these methods. To this end, instead of providing a detailed experimental protocol for each of the steps involved in examining virus marker gene ...


Obesity And Food Choices Among Faculty And Staff At A Large Urban University, Marjorie R. Freedman, Rebecca J. Rubinstein 2009 San Jose State University

Obesity And Food Choices Among Faculty And Staff At A Large Urban University, Marjorie R. Freedman, Rebecca J. Rubinstein

Marjorie R. Freedman

Objective: In order to address increasing health care costs associated with obesity, this study sought to determine prevalence of overweight and obesity and examine eating behaviors, food choices, health beliefs, and attitudes of university employees. Participants and Methods: An online survey was distributed to > 3,800 faculty and staff at a large public metropolitan university in Winter 2008. Results: Almost half (48%) of 806 respondents were classified as overweight or obese. Compared to those with normal weights, overweight and obese respondents consumed fewer fruits and vegetables (p < .05), were less confident in making healthful food choices (p < .001), and were ...


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