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204,358 full-text articles. Page 5171 of 5198.

Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris, Jer-Young Lin, Robert M. Stupar, Christian Hans, D. L. Hyten, Scott A. Jackson 2010 Purdue University

Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris, Jer-Young Lin, Robert M. Stupar, Christian Hans, D. L. Hyten, Scott A. Jackson

Agronomy & Horticulture -- Faculty Publications

Soybean (Glycine max) has undergone at least two rounds of polyploidization, resulting in a paleopolyploid genome that is a mosaic of homoeologous regions. To determine the structural and functional impact of these duplications, we sequenced two ~1-Mb homoeologous regions of soybean, Gm8 and Gm15, derived from the most recent ~13 million year duplication event and the orthologous region from common bean (Phaseolus vulgaris), Pv5. We observed inversions leading to major structural variation and a bias between the two chromosome segments as Gm15 experienced more gene movement (gene retention rate of 81% in Gm15 versus 91% in Gm8) and a nearly ...


Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean, Ki-Seung Kim, Stephanie Bellendir, Karen A. Hudson, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers 2010 University of Illinois

Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean, Ki-Seung Kim, Stephanie Bellendir, Karen A. Hudson, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Agronomy & Horticulture -- Faculty Publications

The soybean aphid (Aphis glycines Matsumura) is an important soybean [Glycine max (L.) Merr.] pest in North America. The dominant aphid resistance gene Rag1 was previously mapped from the cultivar ‘Dowling’ to a 12 cM marker interval on soybean chromosome 7 (formerly linkage group M). The development of additional genetic markers mapping closer to Rag1 was needed to accurately position the gene to improve the effectiveness of markerassisted selection (MAS) and to eventually clone it. The objectives of this study were to identify single nucleotide polymorphisms (SNPs) near Rag1 and to position these SNPs relative to Rag1. To generate a ...


A High Density Integrated Genetic Linkage Map Of Soybean And The Development Of A 1536 Universal Soy Linkage Panel For Quantitative Trait Locus Mapping, D. L. Hyten, Ik-Young Choi, Qijian Song, James E. Specht, Thomas E. Carter Jr., Randy C. Shoemaker, Eun-Young Hwang, Lakshmi K. Matukumalli, P. B. Cregan 2010 USDA-ARS, Soybean Genomics and Improvement Laboratory, Beltsville, Maryland

A High Density Integrated Genetic Linkage Map Of Soybean And The Development Of A 1536 Universal Soy Linkage Panel For Quantitative Trait Locus Mapping, D. L. Hyten, Ik-Young Choi, Qijian Song, James E. Specht, Thomas E. Carter Jr., Randy C. Shoemaker, Eun-Young Hwang, Lakshmi K. Matukumalli, P. B. Cregan

Agronomy & Horticulture -- Faculty Publications

Single nucleotide polymorphisms (SNPs) are the marker of choice for many researchers due to their abundance and the high-throughput methods available for their multiplex analysis. Only recently have SNP markers been available to researchers in soybean [Glycine max (L.) Merr.] with the release of the third version of the consensus genetic linkage map that added 1141 SNP markers to the map. Our objectives were to add 2500 additional SNP markers to the soybean integrated map and select a set of 1536 SNPs to create a universal linkage panel for high-throughput soybean quantitative trait locus (QTL) mapping. The GoldenGate assay is ...


An Integrative Approach To Genomic Introgression Mapping, Andrew J. Severin, Gregory A. Peiffer, Wayne W. Xu, D. L. Hyten, Bruna Bucciarelli, Jamie A. O'Rourke, Yung-Tsi Bolon, David Grant, Andrew D. Farmer, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker, Robert M. Stupar 2010 Iowa State University

An Integrative Approach To Genomic Introgression Mapping, Andrew J. Severin, Gregory A. Peiffer, Wayne W. Xu, D. L. Hyten, Bruna Bucciarelli, Jamie A. O'Rourke, Yung-Tsi Bolon, David Grant, Andrew D. Farmer, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker, Robert M. Stupar

Agronomy & Horticulture -- Faculty Publications

Near-isogenic lines (NILs) are valuable genetic resources for many crop species, including soybean (Glycine max). The development of new molecular platforms promises to accelerate the mapping of genetic introgressions in these materials. Here, we compare some existing and emerging methodologies for genetic introgression mapping: single-feature polymorphism analysis, Illumina GoldenGate single nucleotide polymorphism (SNP) genotyping, and de novo SNP discovery via RNA-Seq analysis of next-generation sequence data. We used these methods to map the introgressed regions in an iron-inefficient soybean NIL and found that the three mapping approaches are complementary when utilized in combination. The comparative RNA-Seq approach offers several additional ...


Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan 2010 USDA-ARS, Soybean Genomics and Improvement Lab., Beltsville, MD

Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan

Agronomy & Horticulture -- Faculty Publications

Simple sequence repeat (SSR) genetic markers, also referred to as microsatellites, function in map-based cloning and for marker-assisted selection in plant breeding. The objectives of this study were to determine the abundance of SSRs in the soybean genome and to develop and test soybean SSR markers to create a database of locus-specific markers with a high likelihood of polymorphism. A total of 210,990 SSRs with di-, tri-, and tetranucleotide repeats of five or more were identified in the soybean whole genome sequence (WGS) which included 61,458 SSRs consisting of repeat units of di- (≥10), tri- (≥8), and tetranucleotide ...


Toxin-Nutrient Interactions Influence Diet Selection, USU Extension 2010 Utah State University

Toxin-Nutrient Interactions Influence Diet Selection, Usu Extension

All Current Publications

Given a choice herbivores prefer foods that are high in nutrients and low in toxins. Nevertheless, plants high in toxins are common on rangelands.


Training Livestock To Leave Streams And Use Uplands, USU Extension 2010 Utah State University

Training Livestock To Leave Streams And Use Uplands, Usu Extension

All Current Publications

Cattle can damage streams and surrounding vegetation—riparian areas—by breaking down banks decreasing water quality, and reducing wildlife living in the stream and on the land.


31p Nmr Phospholipid Profiling Of Soybean Emulsion Recovered From Aqueous Extraction, Linxing Yao, Stéphanie Jung 2010 Iowa State University

31p Nmr Phospholipid Profiling Of Soybean Emulsion Recovered From Aqueous Extraction, Linxing Yao, Stéphanie Jung

Food Science and Human Nutrition Publications

The quantity and composition of phospholipids in full-fat soybean flour, flakes, and extruded flakes and in the cream fraction recovered after aqueous extraction (AEP) and enzyme-assisted aqueous extraction (EAEP) of these substrates were studied with 31P NMR. Extruded flakes had significantly more phosphatidic acid (PA) than flakes and flour prior to aqueous extraction. The PA content of the cream recovered after AEP and EAEP of extruded flakes was similar to that of the starting material, whereas the PA content of the creams from flour and flakes significantly increased. Changes in the PA content could be explained by the action ...


Efficient Methods Of Estimating Switchgrass Biomass Supplies, Marty R. Schmer, Robert B. Mitchell, Kenneth P. Vogel, Walter H. Schacht, David B. Marx 2010 USDA-ARS, University of Nebraska - Lincoln

Efficient Methods Of Estimating Switchgrass Biomass Supplies, Marty R. Schmer, Robert B. Mitchell, Kenneth P. Vogel, Walter H. Schacht, David B. Marx

Agronomy & Horticulture -- Faculty Publications

Switchgrass (Panicum virgatum L.) is being developed as a biofuel feedstock for the United States. Efficient and accurate methods to estimate switchgrass biomass feedstock supply within a production area will be required by biorefineries. Our main objective was to determine the effectiveness of indirect methods for estimating biomass yields and composition of switchgrass fields. Indirect measurements were conducted in eastern Nebraska from 2003 to 2007 in which switchgrass biomass yields were manipulated using three nitrogen rates (0 kg N ha-1, 60 kg N ha-1, and 120 kg N ha-1) and two harvest periods (August and post-killing frost ...


Economic Potential Of Substituting Legumes For Synthetic Nitrogen In Warm Season Perennial Grasses Used For Stocker Cattle Grazing, Maru Kering, John Biermacher, Ryan Reuter, Twain Butler, Job D. Springer, James Rogers, John Blanton, Joe Bouton, John A. Guretzky 2010 The Noble Foundation, Ardmore OK

Economic Potential Of Substituting Legumes For Synthetic Nitrogen In Warm Season Perennial Grasses Used For Stocker Cattle Grazing, Maru Kering, John Biermacher, Ryan Reuter, Twain Butler, Job D. Springer, James Rogers, John Blanton, Joe Bouton, John A. Guretzky

Agronomy & Horticulture -- Faculty Publications

Stocker cattle grazing warm season perennial grasses is an important economic activity in the southern Great Plains. Substantial increases in the price of nitrogen fertilizer is negatively affecting forage producers‟ profitability. Two alternative nitrogen management systems that use annual and perennial legumes have been developed for bermudagrass pastures. The goal of the study is to determine if the legumes systems are more profitable than the conventional practice of applying synthetic sources of nitrogen. Results of the two-year grazing study show that the legume systems could not compete economically with the common practice.


Fertilizer Rate Effects On Forage Yield Stability And Nutrient Uptake Of Midland Bermudagrass, John A. Guretzky, Maru K. Kering, Jagadeesh Mosali, Eddie Funderburg, Jon T. Biermacher 2010 University of Nebraska-Lincoln

Fertilizer Rate Effects On Forage Yield Stability And Nutrient Uptake Of Midland Bermudagrass, John A. Guretzky, Maru K. Kering, Jagadeesh Mosali, Eddie Funderburg, Jon T. Biermacher

Agronomy & Horticulture -- Faculty Publications

Our objectives were to document effects of nitrogen (N), phosphorus (P), and potassium (K) fertilizer rates on forage yields and uptake of N, P, and K by Midland bermudagrass [Cynodon dactylon (L.) Pers.] on a Minco fine, sandy loam in southern Oklahoma. After six years of this long-term experiment, forage yield responses to fertilization were mixed and depended on year. Stability analysis indicated forage yields responded positively to N fertilization during favorable weather conditions but negatively during poor weather conditions. Application of 112 kg N ha−1 provided the best yield stability and mean annual forage yield among treatments, 11 ...


A Putative Rna-Binding Protein Positively Regulates Salicylic Acid–Mediated Immunity In Arabidopsis, Yiping Qi, Kenichi Tsuda, Anna Joe, Masanao Sato, Le V. Nguyen, Jane Glazebrook, James R. Alfano, Jerry D. Cohen, Fumiaki Katagiri 2010 University of Minnesota

A Putative Rna-Binding Protein Positively Regulates Salicylic Acid–Mediated Immunity In Arabidopsis, Yiping Qi, Kenichi Tsuda, Anna Joe, Masanao Sato, Le V. Nguyen, Jane Glazebrook, James R. Alfano, Jerry D. Cohen, Fumiaki Katagiri

Faculty Publications from the Center for Plant Science Innovation

RNA-binding proteins (RBP) can control gene expression at both transcriptional and post-transcriptional levels. Plants respond to pathogen infection with rapid reprogramming of gene expression. However, little is known about how plant RBP function in plant immunity. Here, we describe the involvement of an RBP, Arabidopsis thaliana RNA-binding protein-defense related 1 (AtRBP-DR1; At4g03110), in resistance to the pathogen Pseudomonas syringae pv. tomato DC3000. AtRBP-DR1 loss-of-function mutants showed enhanced susceptibility to P. syringae pv. tomato DC3000. Overexpression of AtRBP-DR1 led to enhanced resistance to P. syringae pv. tomato DC3000 strains and dwarfism. The hypersensitive response triggered by P. syringae pv. tomato DC3000 ...


The Ecology Of Speciation In Bacillus, Nora Connor, Johannes Sikorski, Alejandro P. Rooney, Sarah Kopac, Alexander F. Koeppel, Andrew Burger, Scott G. Cole, Elizabeth B. Perry, Danny Krizanc, Nicholas C. Field, Michele Slaton, Frederick M. Cohan 2010 Wesleyan University

The Ecology Of Speciation In Bacillus, Nora Connor, Johannes Sikorski, Alejandro P. Rooney, Sarah Kopac, Alexander F. Koeppel, Andrew Burger, Scott G. Cole, Elizabeth B. Perry, Danny Krizanc, Nicholas C. Field, Michele Slaton, Frederick M. Cohan

Division III Faculty Publications

No abstract provided.


Effects Of Prescribed Fire And Herbicide Application On Cattle Grazing And Herbage Production From Yellow Bluestem Pastures, S. A. Gunter, R. L. Gillen 2010 USDA, ARS, Southern Plains Range Research Station

Effects Of Prescribed Fire And Herbicide Application On Cattle Grazing And Herbage Production From Yellow Bluestem Pastures, S. A. Gunter, R. L. Gillen

Publications from USDA-ARS / UNL Faculty

Forage management practices influence the quantity and quality of forage available to grazing cattle. The objectives of this experiment were to determine the effects of prescribed fire or herbicides on the density of forbs, the production and nutritive value of the herbage, and the performance of beef steers grazing Yellow bluestem [Bothriochloa ischaemum (L.) Keng var. ischaemum (Hack.) Celarier and Harlan] pastures. The experiment was conducted in northwestern Oklahoma from 1998 through 2000, and the untreated pastures (control) were not burned or sprayed. The prescribed-fire treatment (PFT) occurred annually between March 27 and April 10. The herbicide treatment (HT) consisted ...


Horticulture Of Ribes, K. E. Hummer, A. Dale 2010 USDA Agriculture Research Service

Horticulture Of Ribes, K. E. Hummer, A. Dale

Publications from USDA-ARS / UNL Faculty

The genus Ribes L., known as currants and gooseberries, contains more than 150 diverse species indigenous throughout the northern hemisphere and along the Rocky Mountain, Sierra Nevada and Sierra Madres in North America through mountain ranges of Central America to the Andes in South America. Beginning in the 1400s, four main crop types, black currants (Ribes, subgenus: Ribes, section: Botrycarpum), red and white currants (Ribes, subgenus: Ribes, section: Ribes) and gooseberries (Ribes, subgenus: Grossularia) were domesticated from European species. American and Eurasian species were selected and combined into the germplasm base of European and American breeding programmes in the ...


Thermal And Mechanical Properties Of Compression-Molded Pmdi-Reinforced Pcl/Gluten Composites, Abdellatif Mohamed, Victoria L. Finkenstadt, Sherald H. Gordon, Debra E. Palmquist 2010 USDA-ARS

Thermal And Mechanical Properties Of Compression-Molded Pmdi-Reinforced Pcl/Gluten Composites, Abdellatif Mohamed, Victoria L. Finkenstadt, Sherald H. Gordon, Debra E. Palmquist

Publications from USDA-ARS / UNL Faculty

Many biopolymers and synthetic polymers composites were developed by different researchers for environmental protection and for cost reduction. One of these composites is polycaprolactone (PCL) and vital wheat gluten or wheat flour composites were prepared and compatibilized with polymeric diphenylmethane diisocyanate (pMDI) by blending and compression-molding. PCL/pMDI blend exhibited glass transition (Tg) at -67 °C (0.20 J/g/ °C) and vital gluten at 63 °C (0.45 J/g/ °C), whereas no Tg was recorded for wheat flour. Although Tg was unmistakable for either PCL or gluten, all composite exhibited one Tg, which is ...


Comparison Of Percent Hatch And Fungal Infestation In Channel Catfish Eggs After Copper Sulfate, Diquat Bromide, Formalin, And Hydrogen Peroxide Treatment, Andrew J. Mitchell, David L. Straus, Bradley Farmer, Ray Carter 2010 United States Department of Agriculture

Comparison Of Percent Hatch And Fungal Infestation In Channel Catfish Eggs After Copper Sulfate, Diquat Bromide, Formalin, And Hydrogen Peroxide Treatment, Andrew J. Mitchell, David L. Straus, Bradley Farmer, Ray Carter

Publications from USDA-ARS / UNL Faculty

Reduced survival of fish eggs is often a result of infestation with fungi Saprolegnia spp. However, timely chemical treatments often limit these infestations and increase survival. The effect of copper sulfate pentahydrate (CSP; 10 mg of CSP/L of water), diquat bromide (25 mg of diquat cation/L), formalin (433 mg/L), and hydrogen peroxide (H2O2; 250 mg/L) on percent hatch and fungal infestation in eggs of channel catfish Ictalurus punctatus was compared in two identical trials (five replicates for each chemical treatment per trial). The percent hatch in all chemical treatments was significantly better than ...


Novel Carboxamides As Potential Mosquito Repellents, Alan R. Katritzky, Zuoquan Wang, Svetoslav Slavov, Dimitar A. Dobchev, C. Dennis Hall, Maia Tsikolia, Ulrich R. Bernier, Natasha M. Elejalde, Gary G. Clark, Kenneth J. Linthicum 2010 University of Florida

Novel Carboxamides As Potential Mosquito Repellents, Alan R. Katritzky, Zuoquan Wang, Svetoslav Slavov, Dimitar A. Dobchev, C. Dennis Hall, Maia Tsikolia, Ulrich R. Bernier, Natasha M. Elejalde, Gary G. Clark, Kenneth J. Linthicum

Publications from USDA-ARS / UNL Faculty

A model was developed using 167 carboxamide derivatives, from the United States Department of Agriculture archival database, that were tested as arthropod repellents over the past 60 yr. An artificial neural network employing CODESSA PRO descriptors was used to construct a quantitative structure-activity relationship model for prediction of novel mosquito repellents. By correlating the structure of these carboxamides with complete protection time, a measure of repellency based on duration, 34 carboxamides were predicted as candidate mosquito repellents. There were four additional compounds selected on the basis of their structural similarity to those predicted. The compounds were synthesized either by reaction ...


Structure-Activity Relationships Of 33 Carboxamides As Toxicants Against Female Aedes Aegypti (Diptera: Culicidae), Julia Pridgeon, James J. Becnel, Ulrich R. Bernier, Gary G. Clark, Kenneth J. Linthicum 2010 USDA-ARS

Structure-Activity Relationships Of 33 Carboxamides As Toxicants Against Female Aedes Aegypti (Diptera: Culicidae), Julia Pridgeon, James J. Becnel, Ulrich R. Bernier, Gary G. Clark, Kenneth J. Linthicum

Publications from USDA-ARS / UNL Faculty

Aedes aegypti L. is the primary vector of dengue and yellow fever viruses, and use of aerosolized insecticides is one of the primary ways to control this medically important mosquito. However, few new insecticides have been developed for mosquito control in recent years. As a part of our effort to search for new insecticides to control mosquitoes, toxicities of 33 carboxamides were evaluated against female A. aegypti by topical application. This group included nine different categories of compounds, namely benzamides, phenyl-propenamides, propanamides, butanamides, butenamides, pentanamides, pentenamides, hexanamides, and hexenamides, that exhibited varying levels of toxicity against this mosquito species. The ...


Identification Of Protein Carbonyls In Serum Of The Fetal And Neonatal Pig, Thomas J. Caperna, Amy E. Shannon, Le Ann Blomberg, Wesley M. Garrett, Timothy G. Ramsay 2010 USDA-ARS

Identification Of Protein Carbonyls In Serum Of The Fetal And Neonatal Pig, Thomas J. Caperna, Amy E. Shannon, Le Ann Blomberg, Wesley M. Garrett, Timothy G. Ramsay

Publications from USDA-ARS / UNL Faculty

Oxidation of serum proteins leads to non-reversible carbonyl formation which alters their function and is associated with stress-related disease processes. The primary objective of this study was to quantify and identify oxidized serum proteins in fetal and newborn piglets. Protein carbonyls were converted to hydrazones with dinitrophenyl hydrazine and quantified spectrophotometrically. For identification, serum protein carbonyls were derivatized with biotin hydrazide, separated by 2D PAGE and stained with FITCavidin. Biotin-labeled proteins were excised from gels and identified by mass spectrometry. At birth, carbonyls were determined to be ∼600 pmole/mg serum protein. Fetuses at 50 and 100 days of gestation ...


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