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198,239 full-text articles. Page 4952 of 5011.

Electroporation-Mediated Delivery Of A Naked Dna Plasmid Expressing Vegf To The Porcine Heart Enhances Protein Expression, W. G. Marshall Jr., B. A. Boone, J. D. Burgos, S. I. Gografe, M. K. Baldwin, M. L. Danielson, M. J. Larson, D. R. Caretto, Y. Cruz, B. Ferraro, L. C. Heller, K. E. Ugen, M. J. Jaroszeski, R. Heller 2010 Old Dominion University

Electroporation-Mediated Delivery Of A Naked Dna Plasmid Expressing Vegf To The Porcine Heart Enhances Protein Expression, W. G. Marshall Jr., B. A. Boone, J. D. Burgos, S. I. Gografe, M. K. Baldwin, M. L. Danielson, M. J. Larson, D. R. Caretto, Y. Cruz, B. Ferraro, L. C. Heller, K. E. Ugen, M. J. Jaroszeski, R. Heller

Bioelectrics Publications

Gene therapy is an attractive method for the treatment of cardiovascular disease. However, using current strategies, induction of gene expression at therapeutic levels is often inefficient. In this study, we show a novel electroporation (EP) method to enhance the delivery of a plasmid expressing an angiogenic growth factor (vascular endothelial growth factor, VEGF), which is a molecule previously documented to stimulate revascularization in coronary artery disease. DNA expression plasmids were delivered in vivo to the porcine heart with or without coadministered EP to determine the potential effect of electrically mediated delivery. The results showed that plasmid delivery through EP significantly ...


Ancient Dna Analyses Exclude Humans As The Driving Force Behind Late Pleistocene Musk Ox (Ovibos Moschatus) Population Dynamics, Paula F. Campos, Eske Willerslev, Andrei Sher, Ludovic Orlando, Erik Axelsson, Alexei Tikhonov, Kim Aaris-Sorensen, Alex D. Greenwood, Ralf-Dietrich Kahlke, Pavel Kosintsev 2010 Old Dominion University

Ancient Dna Analyses Exclude Humans As The Driving Force Behind Late Pleistocene Musk Ox (Ovibos Moschatus) Population Dynamics, Paula F. Campos, Eske Willerslev, Andrei Sher, Ludovic Orlando, Erik Axelsson, Alexei Tikhonov, Kim Aaris-Sorensen, Alex D. Greenwood, Ralf-Dietrich Kahlke, Pavel Kosintsev

Biological Sciences Faculty Publications

The causes of the late Pleistocene megafaunal extinctions are poorly understood. Different lines of evidence point to climate change, the arrival of humans, or a combination of these events as the trigger. Although many species went extinct, others, such as caribou and bison, survived to the present. The musk ox has an intermediate story: relatively abundant during the Pleistocene, it is now restricted to Greenland and the Arctic Archipelago. In this study, we use ancient DNA sequences, temporally unbiased summary statistics, and Bayesian analytical techniques to infer musk ox population dynamics throughout the late Pleistocene and Holocene. Our results reveal ...


Apoptosis Initiation And Angiogenesis Inhibition: Melanoma Targets For Nanosecond Pulsed Electric Fields, Xinhua Chen, Juergen F. Kolb, R. James Swanson, Karl H. Schoenbach, Stephen J. Beebe 2010 Old Dominion University

Apoptosis Initiation And Angiogenesis Inhibition: Melanoma Targets For Nanosecond Pulsed Electric Fields, Xinhua Chen, Juergen F. Kolb, R. James Swanson, Karl H. Schoenbach, Stephen J. Beebe

Bioelectrics Publications

Many effective anti-cancer strategies target apoptosis and angiogenesis mechanisms. Applications of non-ionizing, nanosecond pulsed electric fields (nsPEFs) induce apoptosis in vitro and eliminate cancer in vivo; however in vivo mechanisms require closer analysis. These studies investigate nsPEF-induced apoptosis and anti-angiogenesis examined by fluorescent microscopy, immunoblots, and morphology. Six hours after treatment with one hundred 300 ns pulses at 40 kV/cm, cells transiently expressed active caspases indicating that caspase-mediated mechanisms. Three hours after treatment transient peaks in Histone 2AX phosphorylation coincided with terminal deoxynucleotidyl transferase dUTP nick end labeling positive cells and pyknotic nuclei, suggesting caspase-independent mechanisms on nuclei/DNA ...


A Golden Garment? Identifying New Ways Of Looking At The Past Through A Preliminary Report Of Textile Fragments From The Pafos ‘Erotes’ Sarcophagus, Adriana Garcia, Diana Wood Conroy 2010 University of Wollongong

A Golden Garment? Identifying New Ways Of Looking At The Past Through A Preliminary Report Of Textile Fragments From The Pafos ‘Erotes’ Sarcophagus, Adriana Garcia, Diana Wood Conroy

Faculty of Science - Papers (Archive)

A golden garment? A preliminary report of textile fragments from the Pafos ‘Erotes’ Sarcophagus Diana Wood Conroy and Adriana Garcia Remnants of very fine gold thread and reddish fibre were found among bone fragments in the ‘pillow’ end of the interior of the Pafos marble sarcophagus in 2001. The placement of the threads suggested a cloth laid over the upper part of the body. The excavator, Dr Eustathios Raptou has described how the sarcophagus had been looted in antiquity, leaving only one jewel and a finial from what must have been rich funerary goods. The textile fragments demonstrated the opulence ...


Development Of A Regional Habitat Classification Scheme For The Amirante Islands, Seychelles, Sarah Hamylton, Tom Spencer, Annelise Hagan 2010 University of Wollongong

Development Of A Regional Habitat Classification Scheme For The Amirante Islands, Seychelles, Sarah Hamylton, Tom Spencer, Annelise Hagan

Faculty of Science - Papers (Archive)

A collaborative expedition between Khaled bin Sultan Living Oceans Foundation, Cambridge Coastal Research Unit and Seychelles Centre for Marine Research and Technology – Marine Parks Authority (SCMRT-MPA) was conducted to the southern Seychelles, western Indian Ocean, in January 2005. This resulted in a series of habitat maps of the reefs and reef islands of the Amirantes Archipelago, derived from remotely-sensed Compact Airborne Spectrographic Imager (CASI) data. The procedures used in map development, image processing techniques and field survey methods are outlined. Habitat classification, and regional-scale comparisons of relative habitat composition are described. The study demonstrates the use of remote sensing data ...


Carboxymethylated-K-Casein: A Convenient Tool For The Identification Of Polyphenolic Inhibitors Of Amyloid Fibril Formation, John A. Carver, Peter J. Duggan, Heath Ecroyd, Yanqin Liu, Adam G. Meyer, C E. Tranberg 2010 University of Wollongong

Carboxymethylated-K-Casein: A Convenient Tool For The Identification Of Polyphenolic Inhibitors Of Amyloid Fibril Formation, John A. Carver, Peter J. Duggan, Heath Ecroyd, Yanqin Liu, Adam G. Meyer, C E. Tranberg

Faculty of Science - Papers (Archive)

Reduced and carboxymethylated-κ-casein (RCM-κ-CN) is a milk-derived amyloidogenic protein that readily undergoes nucleation-dependent aggregation and amyloid fibril formation via a similar pathway to disease-specific amyloidogenic peptides like amyloid beta (Aβ), which is associated with Alzheimer’s disease. In this study, a series of flavonoids, many known to be inhibitors of Aβ fibril formation, were screened for their ability to inhibit RCM-κ-CN fibrilisation, and the results were compared with literature data on Aβ inhibition. Flavonoids that had a high degree of hydroxylation and molecular planarity gave good inhibition of RCM-κ-CN fibril formation. IC50 values were between 10- and 200-fold higher with ...


Alphab-Crystallin Inhibits The Cell Toxicity Associated With Amyloid Fibril Formation By Kappa-Casein And The Amyloid-Beta Peptide, Francis C. Dehle, Heath Ecroyd, Ian F. Musgrave, John A. Carver 2010 University of Adelaide

Alphab-Crystallin Inhibits The Cell Toxicity Associated With Amyloid Fibril Formation By Kappa-Casein And The Amyloid-Beta Peptide, Francis C. Dehle, Heath Ecroyd, Ian F. Musgrave, John A. Carver

Faculty of Science - Papers (Archive)

Amyloid fibril formation is associated with diseases such as Alzheimer’s, Parkinson’s, and prion diseases. Inhibition of amyloid fibril formation by molecular chaperone proteins, such as the small heat-shock protein αB-crystallin, may play a protective role in preventing the toxicity associated with this form of protein misfolding. Reduced and carboxymethylated κ-casein (RCMκ-CN), a protein derived from milk, readily and reproducibly forms fibrils at physiological temperature and pH. We investigated the toxicity of fibril formation by RCMκ-CN using neuronal model PC12 cells and determined whether the inhibition of fibril formation altered its cell toxicity. To resolve ambiguities in the literature ...


Protein Chemistry Of Amyloid Fibrils And Chaperones: Implications For Amyloid Formation And Disease, Justin J. Yerbury, Janet R. Kumita 2010 University of Wollongong

Protein Chemistry Of Amyloid Fibrils And Chaperones: Implications For Amyloid Formation And Disease, Justin J. Yerbury, Janet R. Kumita

Faculty of Science - Papers (Archive)

Understanding the mechanisms by which amyloid fibrils are formed, both in vivo and in vitro, is vital for developing methods to treat and prevent debilitating deposition diseases such as Alzheimer's disease, Parkinson's disease, type II diabetes and systemic amyloidoses. In recent years, computer modelling and biophysical studies have broadened our understanding of the biochemical mechanisms underpinning protein aggregation. As a result, it is now believed that the ability to form fibrils is an intrinsic property of polypeptide chains and not isolated to disease-related proteins or peptides. Molecular chaperones are a diverse group of functionally related proteins well known ...


Interpreting New England Subduction Complex Rocks Using Deep-Sea Drilling Results From The Nankai Trough (Offshore Southwest Japan), Christopher L. Fergusson 2010 University of Wollongong

Interpreting New England Subduction Complex Rocks Using Deep-Sea Drilling Results From The Nankai Trough (Offshore Southwest Japan), Christopher L. Fergusson

Faculty of Science - Papers (Archive)

Two distinct structural styles are considered likely to have affected parts of the subduction complex of the New England Orogen. Imbricate thrusting, as occurs by duplexing at a ramp along the décollement at the base of the subduction complex, accounts for the structure of units such as the Gundahl Complex (also the Wandilla Formation of central Queensland and the Sandon beds of the Armidale district). These units have relatively straight-forward imbricate structure although in the case of the Gundahl Complex the thickness of units is condensed and reflects factors controlling deposition on the ocean plate and in the trench. In ...


And Then There Were None?, Richard G. Roberts, Barry W. Brook 2010 University of Wollongong

And Then There Were None?, Richard G. Roberts, Barry W. Brook

Faculty of Science - Papers (Archive)

No abstract provided.


Age And Origin Of Alluvial Sediments Within And Flanking The Mt Lofty Ranges, Southern South Australia: A Late Quaternary Archive Of Climate And Environmental Change, D Banerjee, N F. Alley, R P. Bourman, S Buckman, J R. Prescott 2010 University of Adelaide

Age And Origin Of Alluvial Sediments Within And Flanking The Mt Lofty Ranges, Southern South Australia: A Late Quaternary Archive Of Climate And Environmental Change, D Banerjee, N F. Alley, R P. Bourman, S Buckman, J R. Prescott

Faculty of Science - Papers (Archive)

No abstract provided.


Detection And Quantification Of Tear Phospholipids And Cholesterol In Contact Lens Deposits: The Effect Of Contact Lens Material And Lens Care Solution, Jennifer Saville, Zhenjun Zhao, Mark D.P. Willcox, Stephen J. Blanksby, Todd W. Mitchell 2010 University of Wollongong

Detection And Quantification Of Tear Phospholipids And Cholesterol In Contact Lens Deposits: The Effect Of Contact Lens Material And Lens Care Solution, Jennifer Saville, Zhenjun Zhao, Mark D.P. Willcox, Stephen J. Blanksby, Todd W. Mitchell

Faculty of Science - Papers (Archive)

PURPOSE. To examine the deposition of tear phospholipids and cholesterol onto worn contact lenses and the effect of lens material and lens care solution. METHODS. Lipids were extracted from tears and worn contact lenses using 2: 1 chloroform: methanol and the extract washed with aqueous ammonium acetate, before analysis by electrospray ionization tandem mass spectrometry (ESI-MS/MS). RESULTS. Twenty-three molecular lipids from the sphingomyelin (SM) and phosphatidylcholine (PC) classes were detected in tears, with total concentrations of each class determined to be 5 +/- 1 pmol/mu L (similar to 3.8 mu g/mL) and 6 +/- 1 pmol/mu L ...


A Mass Spectrometric Investigation Of Novel Quadruplex Dna-Selective Berberine Derivatives, Karina Gornall, Siritron Samosorn, Bongkot Tanwirat, Apichart Suksamrarn, John B. Bremner, Michael J. Kelso, Jennifer L. Beck 2010 University of Wollongong

A Mass Spectrometric Investigation Of Novel Quadruplex Dna-Selective Berberine Derivatives, Karina Gornall, Siritron Samosorn, Bongkot Tanwirat, Apichart Suksamrarn, John B. Bremner, Michael J. Kelso, Jennifer L. Beck

Faculty of Science - Papers (Archive)

ESI mass spectrometry was used to assess the binding of 13-substituted, 5-nitro-2-phenylindolyl- and 2-naphthalenyl-based berberine derivatives to inter- and intramolecular G-quadruplex DNA molecules. In contrast with the parent berberine, the compounds showed selectivity for quadruplex over duplex DNA and stabilised the quadruplex structure. They represent a new class of quadruplex DNA-selective ligands. © 2010 The Royal Society of Chemistry.


A Combined Experimental And Theoretical Study Of Ion Solvation In Liquid N-Methylacetamide, Haibo Yu, Christopher L. Mazzanti, Troy W. Whitfield, Roger E. Koeppe II, Olaf S. Andersen, Benoit Roux 2010 University of Wollongong

A Combined Experimental And Theoretical Study Of Ion Solvation In Liquid N-Methylacetamide, Haibo Yu, Christopher L. Mazzanti, Troy W. Whitfield, Roger E. Koeppe Ii, Olaf S. Andersen, Benoit Roux

Faculty of Science - Papers (Archive)

Most current biomolecular simulations are based on potential energy functions that treat the electrostatic energy as a sum of pairwise Coulombic interactions between effective fixed atomic charges. This approximation, in which many-body induced polarization effects are included in an average way, is expected to be satisfactory for a wide range of systems, but less accurate for processes involving the transfer and partition of ions among heterogeneous environments. The limitations of these potential energy functions are perhaps most obvious in studies of ion permeation through membrane channels. In many cases, the pore is so narrow that the permeating ion must shed ...


Elastic Scattering Studies Of Aligned Dmpc Multilayers On Different Hydrations, Marcus Trapp, Fanni Juranyi, Moeava Tehei, Lambert van Eijck, Bruno Demé, Thomas Gutberlet, Judith Peters 2010 Institut de Biologie Structurale, France

Elastic Scattering Studies Of Aligned Dmpc Multilayers On Different Hydrations, Marcus Trapp, Fanni Juranyi, Moeava Tehei, Lambert Van Eijck, Bruno Demé, Thomas Gutberlet, Judith Peters

Faculty of Science - Papers (Archive)

Biological membranes, consisting mainly of phospholipids and proteins, are organized in a bilayered structure which exhibits dynamical behaviour within time regimes ranging from 10-12 s with the motion of alkyl chain defects and 1 s corresponding to collective excitations of the bilayer [1]. With the prominent role hydration plays on the structural phase behaviour of phospholipids membranes, it is essential for a better description of membranes to understand also the influence of hydration on the dynamics of membrane systems. In the present study we have performed neutron scattering investigations on highly oriented DMPC-d54 multilayers at two different relative humidity (rh ...


Catchments And Waterways, Bob Bourman, Nick Harvey, Simon Bryars 2010 University of Wollongong

Catchments And Waterways, Bob Bourman, Nick Harvey, Simon Bryars

Faculty of Science - Papers (Archive)

No abstract provided.


The Nature Of Flooding, Robert P. Bourman 2010 University of Wollongong

The Nature Of Flooding, Robert P. Bourman

Faculty of Science - Papers (Archive)

No abstract provided.


Management Of Amphibian Populations In Booderee National Park, South-Eastern Australia, Trent D. Penman, Traecey Brassil 2010 University of Wollongong

Management Of Amphibian Populations In Booderee National Park, South-Eastern Australia, Trent D. Penman, Traecey Brassil

Faculty of Science - Papers (Archive)

Often land set aside for conservation becomes a multiple use area, which forces land managers to balance biodiversity values against competing needs. Booderee National Park is an important conservation reserve for a range of amphibian species in south-eastern Australia. The Park includes a number of townships, defence facilities, and recreation areas, as well as land for conservation. We examined amphibian communities in the area and related these to broad habitat features and identified potential threats to the long term viability of these populations. Two distinct assemblages occurred within the Park that could be related to broad habitat features of the ...


Soil Geochemistry And Pathfinder Element Distribution Associated With The Hillgrove Antimony-Gold-Tungsten Deposit, New England Orogen, Nsw, R Ellsmore, Solomon Buckman, C Simpson 2010 University of Wollongong

Soil Geochemistry And Pathfinder Element Distribution Associated With The Hillgrove Antimony-Gold-Tungsten Deposit, New England Orogen, Nsw, R Ellsmore, Solomon Buckman, C Simpson

Faculty of Science - Papers (Archive)

No abstract provided.


Parcel Subdivision Automation For Agent-Based Land Use Modelling, Rohan Wickramasuriya, Laurie Chisholm, Marji Puotinen, Nicholas Gill, Peter Klepeis 2010 University of Wollongong

Parcel Subdivision Automation For Agent-Based Land Use Modelling, Rohan Wickramasuriya, Laurie Chisholm, Marji Puotinen, Nicholas Gill, Peter Klepeis

Faculty of Science - Papers (Archive)

To a significant extent rural Australia is transforming into multifunctional landscapes. Amenity migration (i.e. movement of people from metropolitan to rural settings) is a major driving force of this transition in many areas. However, the effects of amenity migration on the receiving landscapes are not yet fully understood. Agent-based land use modelling helps unravel the complex spatio-temporal relationships that affect landscape response to change from amenity migration. A land subdivision module is essential for a complete agent-based land use model developed for these landscapes because the land sold to in-migrants are lots that are subdivided from much larger tracts ...


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