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Salmon Fibrin Supports An Increased Number Of Sprouts And Decreased Degradation While Maintaining Sprout Length Relative To Human Fibrin In An In Vitro Angiogenesis Model, Alisha Sarang-Sieminski, Keith Gooch 2012 Franklin W. Olin College of Engineering

Salmon Fibrin Supports An Increased Number Of Sprouts And Decreased Degradation While Maintaining Sprout Length Relative To Human Fibrin In An In Vitro Angiogenesis Model, Alisha Sarang-Sieminski, Keith Gooch

Alisha L. Sarang-Sieminski

Salmon-derived fibrin has been proposed as a preferred alternative to human or bovine fibrin because of its reduced potential for disease transmission. Here we evaluate salmon fibrin as an alternative ECM support for therapeutic angiogenesis applications, such as vascularizing engineered tissues. Human umbilical vein endothelial cells (HUVEC) seeded on gelatin beads and suspended in either salmon or human fibrin sprouted and formed capillary-like structures. Sprout length was generally increased with the addition of bFGF and VEGF and further increased with the addition of phorbol myristate acetate (PMA). The number of sprouts per bead was increased 61-188% in salmon fibrin relative ...


Migration Of Tumor Cells In 3d Matrices Is Governed By Matrix Stiffness Along With Cell-Matrix Adhesion And Proteolysis, Alisha Sarang-Sieminski, Muhammad Zaman, Linda Trapani, Drew MacKellar, Haiyan Gong, Roger Kamm, Alan Wells, Douglas Lauffenburger, Paul Matsudaira 2012 Franklin W. Olin College of Engineering

Migration Of Tumor Cells In 3d Matrices Is Governed By Matrix Stiffness Along With Cell-Matrix Adhesion And Proteolysis, Alisha Sarang-Sieminski, Muhammad Zaman, Linda Trapani, Drew Mackellar, Haiyan Gong, Roger Kamm, Alan Wells, Douglas Lauffenburger, Paul Matsudaira

Alisha L. Sarang-Sieminski

Cell migration on 2D surfaces is governed by a balance between counteracting tractile and adhesion forces. Although biochemical factors such as adhesion receptor and ligand concentration and binding, signaling through cell adhesion complexes, and cytoskeletal structure assembly/disassembly have been studied in detail in a 2D context, the critical biochemical and biophysical parameters that affect cell migration in 3D matrices have not been quantitatively investigated. We demonstrate that, in addition to adhesion and tractile forces, matrix stiffness is a key factor that influences cell movement in 3D. Cell migration assays in which Matrigel density, fibronectin concentration, and β1 integrin binding ...


Analysis Of Migration Models Of Biogeography-Based Optimization Using Markov Theory, Haiping Ma, Daniel J. Simon 2012 Shaoxing University, Shaoxing, China

Analysis Of Migration Models Of Biogeography-Based Optimization Using Markov Theory, Haiping Ma, Daniel J. Simon

Daniel J. Simon

Biogeography-based optimization (BBO) is a new evolutionary algorithm inspired by biogeography, which involves the study of the migration of biological species between habitats. Previous work has shown that various migration models of BBO result in significant changes in performance. Sinusoidal migration models have been shown to provide the best performance so far. Motivated by biogeography theory and previous results, in this paper a generalized sinusoidal migration model curve is proposed. A previously derived BBO Markov model is used to analyze the effect of migration models on optimization performance, and new theoretical results which are confirmed with simulation results are obtained ...


Blended Biogeography-Based Optimization For Constrained Optimization, Haiping Ma, Daniel J. Simon 2012 Shaoxing University, Shaoxing, China

Blended Biogeography-Based Optimization For Constrained Optimization, Haiping Ma, Daniel J. Simon

Daniel J. Simon

Biogeography-based optimization (BBO) is a new evolutionary optimization method that is based on the science of biogeography. We propose two extensions to BBO. First, we propose a blended migration operator. Benchmark results show that blended BBO outperforms standard BBO. Second, we employ blended BBO to solve constrained optimization problems. Constraints are handled by modifying the BBO immigration and emigration procedures. The approach that we use does not require any additional tuning parameters beyond those that are required for unconstrained problems. The constrained blended BBO algorithm is compared with solutions based on a stud genetic algorithm (SGA) and standard particle swarm ...


Analysis Of Saint John's Maple Syrup Records: 1942-2009, Stephen G. Saupe 2012 College of Saint Benedict/Saint John's University

Analysis Of Saint John's Maple Syrup Records: 1942-2009, Stephen G. Saupe

Stephen G. Saupe

No abstract provided.


Teaching Stress Physiology Using Zebrafish (Danio Rerio), Michael Cooper, Shree Dhawale, Ahmed Mustafa 2012 Indiana University Purdue University Fort Wayne

Teaching Stress Physiology Using Zebrafish (Danio Rerio), Michael Cooper, Shree Dhawale, Ahmed Mustafa

Ahmed Mustafa Dr.

A straightforward and inexpensive laboratory experiment is presented that investigates the physiological stress response of zebrafish after a 5 °C increase in water temperature. This experiment is designed for an undergraduate physiology lab and allows students to learn the scientific method and relevant laboratory techniques without causing significant stress to animals. An additional experimental design and a set of additional questions for lab report are also included.


Maple Syrup: St. John’S Sweetest Springtime Tradition, Stephen G. Saupe 2012 College of Saint Benedict and Saint John's University

Maple Syrup: St. John’S Sweetest Springtime Tradition, Stephen G. Saupe

Biology Faculty Publications

St. John’s is the home of one of Minnesota’s oldest maple syrup operations. The monks began making syrup in 1942 and have continued roughly every other spring until the present. Currently, the operation is jointly run by the Abbey and St. John’s Arboretum and it is one of the few maple syrup operations associated with a Minnesota college or university. The process by which maple syrup is made at St. John’s differs little from the procedures begun more than 60 years ago. In spring, sugar maple trees are tapped, sap is collected, and then it is ...


Nutrient Mass Balance And Performance Of Feedlot Cattle Fed Barley Based Diets In Large Pens In Western Canada, Erin M. Hussey 2012 University of Nebraska-Lincoln

Nutrient Mass Balance And Performance Of Feedlot Cattle Fed Barley Based Diets In Large Pens In Western Canada, Erin M. Hussey

Theses and Dissertations in Animal Science

Three separate large pen commercial feeding trials with approximately 9,000 heifers with either eight or ten reps/treatment were conducted at a Western Feedlots Ltd.- High River, a commercial feedyard near High River, AB, Canada. For all three experiments, Near Infrared Spectroscopy (NIRS) was used to segregate feed barley. In Exp. 1, barley was segregated based on digestible energy (DE) content as predicted by NIRS and heifers were fed low or high DE barley, or a 50:50 blend of the two. Feeding low DE barley improved weight gain, dry matter intake (DMI), and mortality; with little effect on ...


Polyglutamine Toxicity Is Controlled By Prion Composition And Gene Dosage In Yeast, He Gong, Nina V. Romanova, Kim D. Allen, Pavithra Chandramowlishwaran, Kavita Gokhale, Gary P. Newnam, Piotr MIceczkowski, Michael Y. Sherman, Yury O. Chernoff 2012 Georgia Institute of Technology

Polyglutamine Toxicity Is Controlled By Prion Composition And Gene Dosage In Yeast, He Gong, Nina V. Romanova, Kim D. Allen, Pavithra Chandramowlishwaran, Kavita Gokhale, Gary P. Newnam, Piotr Miceczkowski, Michael Y. Sherman, Yury O. Chernoff

Publications and Research

Polyglutamine expansion causes diseases in humans and other mammals. One example is Huntington’s disease. Fragments of human huntingtin protein having an expanded polyglutamine stretch form aggregates and cause cytotoxicity in yeast cells bearing endogenous QN-rich proteins in the aggregated (prion) form. Attachment of the proline(P)-rich region targets polyglutamines to the large perinuclear deposit (aggresome). Aggresome formation ameliorates polyglutamine cytotoxicity in cells containing only the prion form of Rnq1 protein. Here we show that expanded polyglutamines both with (poly-QP) or without (poly-Q) a P-rich stretch remain toxic in the presence of the prion form of translation termination (release ...


Paramyxovirus Fusion And Entry: Multiple Paths To A Common End, Andres Chang, Rebecca Ellis Dutch 2012 University of Kentucky

Paramyxovirus Fusion And Entry: Multiple Paths To A Common End, Andres Chang, Rebecca Ellis Dutch

Molecular and Cellular Biochemistry Faculty Publications

The paramyxovirus family contains many common human pathogenic viruses, including measles, mumps, the parainfluenza viruses, respiratory syncytial virus, human metapneumovirus, and the zoonotic henipaviruses, Hendra and Nipah. While the expression of a type 1 fusion protein and a type 2 attachment protein is common to all paramyxoviruses, there is considerable variation in viral attachment, the activation and triggering of the fusion protein, and the process of viral entry. In this review, we discuss recent advances in the understanding of paramyxovirus F protein-mediated membrane fusion, an essential process in viral infectivity. We also review the role of the other surface glycoproteins ...


Loss Of Anti-Viral Immunity By Infection With A Virus Encoding A Cross-Reactive Pathogenic Epitope, Alex T. Chen, Markus Cornberg, Stephanie Gras, Carole Guillonneau, Jamie Rossjohn, Andrew Trees, Sebastien Emonet, Juan C. de la Torre, Raymond M. Welsh, Liisa K. Selin 2012 University of Massachusetts Medical School

Loss Of Anti-Viral Immunity By Infection With A Virus Encoding A Cross-Reactive Pathogenic Epitope, Alex T. Chen, Markus Cornberg, Stephanie Gras, Carole Guillonneau, Jamie Rossjohn, Andrew Trees, Sebastien Emonet, Juan C. De La Torre, Raymond M. Welsh, Liisa K. Selin

Open Access Articles

T cell cross-reactivity between different strains of the same virus, between different members of the same virus group, and even between unrelated viruses is a common occurrence. We questioned here how an intervening infection with a virus containing a sub-dominant cross-reactive T cell epitope would affect protective immunity to a previously encountered virus. Pichinde virus (PV) and lymphocytic choriomeningitis virus (LCMV) encode subdominant cross-reactive NP(2)(0)(5)(-)(2)(1)(2) CD8 T cell epitopes sharing 6 of 8 amino acids, differing only in the MHC anchoring regions. These pMHC epitopes induce cross-reactive but non-identical T cell receptor (TCR) repertoires ...


Mixed Methods Analysis Of School Wellness Programs In Nebraska And Indiana: A Descriptive Study, Jessica A. Robinson 2012 University of Nebraska-Lincoln

Mixed Methods Analysis Of School Wellness Programs In Nebraska And Indiana: A Descriptive Study, Jessica A. Robinson

Nutrition & Health Sciences Dissertations & Theses

Schools have become one of the major fronts that the battle against childhood obesity is being fought. In 2004 the Child Nutrition Act passed by Congress required all school districts receiving funds for the National School Lunch Program to create a wellness policy. Since then there have been many research projects conducted to learn more about these policies, and their implementation as programs in the schools. Thus far, no such research has been done in Nebraska or Indiana. The purpose of this descriptive study is to describe how schools in Nebraska and Indiana are implementing wellness programs in their schools ...


Effect Of Torrefaction On Water Vapor Adsorption Properties And Resistance To Microbial Degradation Of Corn Stover, Dorde Medic, Matthew J. Darr, Ajay Shah, Sarah Jane Rahn 2012 Iowa State University

Effect Of Torrefaction On Water Vapor Adsorption Properties And Resistance To Microbial Degradation Of Corn Stover, Dorde Medic, Matthew J. Darr, Ajay Shah, Sarah Jane Rahn

Agricultural and Biosystems Engineering Publications

The equilibrium moisture content (EMC) of biomass affects transportation, storage, downstream feedstock processing, and the overall economy of biorenewables production. Torrefaction is a thermochemical process conducted in the temperature regime between 200 and 300 °C under an inert atmosphere that, among other benefits, aims to reduce the innate hydrophilicity and susceptibility to microbial degradation of biomass. The objective of this study was to examine water sorption properties of torrefied corn stover. The EMC of raw corn stover, along with corn stover thermally pretreated at three temperatures, was measured using the static gravimetric method at equilibrium relative humidity (ERH) and temperatures ...


Mass Spectrometry-Based Proteomics Analysis Of The Matrix Microenvironment In Pluripotent Stem Cell Culture, Christopher Hughes 2012 The University of Western Ontario

Mass Spectrometry-Based Proteomics Analysis Of The Matrix Microenvironment In Pluripotent Stem Cell Culture, Christopher Hughes

Electronic Thesis and Dissertation Repository

The stem cell microenvironment contains soluble factors, support cells, and components of the extracellular matrix (ECM) that combine to effect cellular behavior. Mass spectrometry based proteomics offers the opportunity to directly assay components of extracellular microenvironments, thereby providing a sensitive means for obtaining insight into the stem cell niche. In this study we present the generation and analysis of human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) matrix microenvironments using an MS-based proteomics approach.

One of the primary limitations in the proteomics analysis of hESCs and hiPSCs is the reproducible generation of sufficient cell numbers amenable ...


The Application Of Social/Political Theories To The Spliss (Sport Policy Factors Leading To International Sporting Success) Model, Winston Wing Hong To, Bryn Jones, Juliann Desjardins, Darwin Semotiuk 2012 Western University

The Application Of Social/Political Theories To The Spliss (Sport Policy Factors Leading To International Sporting Success) Model, Winston Wing Hong To, Bryn Jones, Juliann Desjardins, Darwin Semotiuk

Winston Wing Hong To

This research focuses if a sociocultural theory such as the stakeholder theory or social value theory can be applicable to enhance the theoretical explanation of the SPLISS (Sport Policy Factors Leading to International Sporting Success) model. The SPLISS model is a comparative high performance sport (HPS) model which was created by researchers from Belgium, United Kingdom, and Netherlands in 2002 with the purpose of seeking which factors will lead countries to international sporting success (e.g. Olympics, Paralympics, World Championships) (Legg & DeBosscher, nd). A comparative HPS model is a research model that has a set of ingredients composed by academic researcher(s) to compare different HPS systems (To & Smolianov & Semotiuk, under review). A HPS system is the communication or non-communication and organization of stakeholders (such as athletes, coaches, organizations, government) that focuses on HPS within a country (To et al., under review).

The SPLISS model is based on the ideology of world competitiveness that is routinely used in economic studies (De Bosscher & Shibli & van Bottenburg & De Knop & Truyens, 2010). The theoretical framework used to develop the SPLISS model is based on the Porter’s five forces analysis framework developed by Michael Porter in 1979; threat of new entrants, bargaining power of suppliers, threat of substitute products or services, bargaining power of buyers, rivalry ...


Influence Of Prednisolone On Urinary Calcium Oxalate And Struvite Relative Supersaturation In Healthy Young Adult Female Domestic Shorthaired Cats, N Geyer, Joe Bartges, Claudia Kirk, Sherry Cox, A Hezel, T Moyers, J Hayes 2012 University of Tennessee, Knoxville

Influence Of Prednisolone On Urinary Calcium Oxalate And Struvite Relative Supersaturation In Healthy Young Adult Female Domestic Shorthaired Cats, N Geyer, Joe Bartges, Claudia Kirk, Sherry Cox, A Hezel, T Moyers, J Hayes

Sherry Cox

Prednisolone (10 mg PO q24h) or placebo was administered to healthy cats for 2 weeks in a masked, placebo-controlled, crossover-design study, and 24-hour urine samples were collected. When cats received prednisolone, 24-hour urine pH was lower and 24-hour urine excretion of creatinine, magnesium, phosphate, and potassium was higher than when cats received placebo. No significant difference was found in urinary relative supersaturation for calcium oxalate (CaOx) or struvite between treatment groups. Prednisolone administration did not induce diuresis, nor was it associated with increased calcium excretion or urinary saturation for CaOx in these healthy cats. Results of this study, however, should ...


Influence Of Hydrochlorothiazide On Urinary Calcium Oxalate Relative Supersaturation In Healthy Young Adult Female Domestic Shorthaired Cat, A Hezel, Joe Bartges, Claudia Kirk, Sherry Cox, N Geyer, T Moyers, J Hayes 2012 University of Tennessee, Knoxville

Influence Of Hydrochlorothiazide On Urinary Calcium Oxalate Relative Supersaturation In Healthy Young Adult Female Domestic Shorthaired Cat, A Hezel, Joe Bartges, Claudia Kirk, Sherry Cox, N Geyer, T Moyers, J Hayes

Sherry Cox

Hydrochlorothiazide (1 mg/kg PO q12h) or placebo was administered to healthy cats for 2 weeks in a masked, placebo-controlled, crossover-design study, and 24-hour urine samples were collected. When cats received hydrochlorothiazide, 24-hour urine volume, ammonia, chloride, creatinine, magnesium, oxalic acid, phosphate, potassium, and sodium were significantly higher than when cats received placebo. Hydrochlorothiazide was associated with significantly lower urinary saturation for calcium oxalate, but no difference was found in 24-hour urine calcium and citrate, urinary saturation for struvite, or blood ionized calcium. Hydrochlorothiazide decreased urinary saturation for calcium oxalate and could be useful in managing cats with calcium oxalate ...


Thermal Conductivity Of Bovine Serum Albumin: A Tool To Probe Denaturation Of Protein, Byoung Kyoo Park, Namwoo Yi, Jaesung Park, Tae Y. Choi, Jin Young Lee, Ahmed Busnaina, Dongsik Kim 2012 POSTECH, South Korea

Thermal Conductivity Of Bovine Serum Albumin: A Tool To Probe Denaturation Of Protein, Byoung Kyoo Park, Namwoo Yi, Jaesung Park, Tae Y. Choi, Jin Young Lee, Ahmed Busnaina, Dongsik Kim

Ahmed A. Busnaina

We demonstrate a strong correlation between denaturation of bovine serum albumin (BSA) and the thermal conductivity k of aqueous solutions of BSA. When denaturation of BSA began, k dropped significantly. These results suggest that k, i.e., the ability of a protein to transport passively applied thermal energy, can be exploited to probe the conformational dynamics of BSA and potentially of other proteins. The technique of protein analysis demonstrated in this work is expected to be useful in micro-total-analysis systems because it is easier to miniaturize and to integrate into a device than is conventional differential scanning calorimetry analysis.


Development Of Markets For Local Food Crop To Enhance Incomes And Improve Food Security For Smallholder Farmers In East Africa, Betty Bugusu 2012 Purdue University

Development Of Markets For Local Food Crop To Enhance Incomes And Improve Food Security For Smallholder Farmers In East Africa, Betty Bugusu

Changing World Conference

No abstract provided.


A Unified View Of Base Excision Repair, Karen Almeida, Robert Sobol 2012 Rhode Island College

A Unified View Of Base Excision Repair, Karen Almeida, Robert Sobol

Karen H Almeida

Base excision repair (BER) proteins act upon a significantly broad spectrum of DNA lesions that result from endogenous and exogenous sources. Multiple sub-pathways of BER (short-path or longpatch) and newly designated DNA repair pathways (e.g., SSBR and NIR) that utilize BER proteins complicate any comprehensive understanding of BER and its role in genome maintenance, chemotherapeutic response, neurodegeneration, cancer or aging. Herein, we propose a unified model of BER, comprised of three functional processes: Lesion Recognition/Strand Scission, Gap Tailoring and DNA Synthesis/Ligation, each represented by one or more multiprotein complexes and coordinated via the XRCC1/DNA Ligase III ...


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