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Characterization Of Regulatory Mechanisms For Alternative Splicing In Alpha Thyroid Hormone Receptor Mrna, Chao Zhang 2010 Marquette University

Characterization Of Regulatory Mechanisms For Alternative Splicing In Alpha Thyroid Hormone Receptor Mrna, Chao Zhang

Master's Theses (2009 -)

In eukaryotes, alternative splicing is an essential post transcriptional modification process for functional gene expression and a major contributor to protein diversity. The regulation of alternative splicing generally involves the engagements of RNA sequences cis-acting elements) and corresponding protein factors (trans-acting factors). The cisacting RNA motifs can be categorized depending on positional and functional differences. Trans-acting protein factors will then bind to RNA sequences and affect the corresponding splicing activity. Recently, factors associated with 3’ polyadenylation have also been identified to affect alternative splicing.

In mammals, the α-thyroid hormone receptor gene (TRα) produces transcripts for two functionally antagonistic isoforms, TRα1 ...


The Effect Of Natural Antimicrobial Ingredients On The Quality Of Roast Beef And Oven Roasted Turkey, Ace F. VanDeWalle 2010 University of Nebraska at Lincoln

The Effect Of Natural Antimicrobial Ingredients On The Quality Of Roast Beef And Oven Roasted Turkey, Ace F. Vandewalle

Theses and Dissertations in Animal Science

This research evaluates the use of a buffered vinegar and lemon juice concentrate (LV) solution on the quality of restructured beef and turkey rolls. Two studies were conducted to evaluate processing and ingredient concentration. Beef and turkey rolls were formulated with minimal ingredients. 1.5% sea salt, 0.5% turbinado sugar, with LV or no LV. Study one evaluated length of mixing time (2.5, 5, 10, 15, 20 minutes) on quality and sensory attributes with 2.5% LV or 0% LV (C). Study two was divided into four treatments, 0% (C), 1.5%, 2.5%, and 3.5% LV ...


Interaction Between Roughages And Corn Milling Byproducts In Finishing Cattle Diets, Joshua R. Benton 2010 University of Nebraska at Lincoln

Interaction Between Roughages And Corn Milling Byproducts In Finishing Cattle Diets, Joshua R. Benton

Theses and Dissertations in Animal Science

During ethanol production, starch is the primary nutrient fermented and the remaining byproducts are excellent sources of fiber and protein. In addition, inclusion of byproducts in finishing diets may reduce the incidence of acidosis. As a result, roughage level and quality could potentially be reduced in finishing diets containing byproducts. Three experiments were conducted to examine the effects of roughage and wet corn gluten feed (WCGF) in finishing cattle diets containing corn distillers grains plus solubles. Cattle fed finishing diets containing wet distillers grains plus solubles (WDGS) with no roughage had decreased DMI and ADG compared to cattle fed roughage ...


Aryl Boronic Acid Inhibition Of Synthetic Melanin Polymerization, Jason M. Belitsky 2010 Oberlin College

Aryl Boronic Acid Inhibition Of Synthetic Melanin Polymerization, Jason M. Belitsky

Faculty & Staff Scholarship

Inhibitors of melanin formation are sought after for a range of applications. Boronophenylalanine is known to inhibit melanogenesis via boronic acid-catechol interactions. A spectroscopic assay was developed to study the polymerization of L-dopa to synthetic melanin in the presence of para-substituted aryl boronic acids. The best inhibition was observed for aryl boronic acids with electron-withdrawing substituents. The IC50 values exhibit a correlation with the Hammett sigma(p) parameter (rho = 0.97, r(2) = 0.92).


Neutralization Of Vascular Endothelial Growth Factor Antiangiogenic Isoforms Or Administration Of Proangiogenic Isoforms Stimulates Vascular Development In The Rat Testis, Michelle M. Baltes-Breitwisch, Robin A. Artac, Rebecca C. Bott, Renee M. McFee, Jill G. Kerl, Debra T. Clopton, Andrea S. Cupp 2010 University of Nebraska-Lincoln

Neutralization Of Vascular Endothelial Growth Factor Antiangiogenic Isoforms Or Administration Of Proangiogenic Isoforms Stimulates Vascular Development In The Rat Testis, Michelle M. Baltes-Breitwisch, Robin A. Artac, Rebecca C. Bott, Renee M. Mcfee, Jill G. Kerl, Debra T. Clopton, Andrea S. Cupp

Faculty Papers and Publications in Animal Science

Vascular endothelial growth factor A (VEGFA) plays a role in both angiogenesis and seminiferous cord formation, and alternative splicing of the Vegfa gene produces both proangiogenic isoforms and antiangiogenic isoforms (B-isoforms). The objectives of this study were to evaluate the expression of pro- and antiangiogenic isoforms during testis development and to determine the role of VEGFA isoforms in testis morphogenesis. Quantitative RT-PCR determined that Vegfa_165b mRNA was most abundant between embryonic days 13.5 and 16 (E13.5 and 16; P < 0.05). Compared with ovarian mRNA levels, Vegfa_120 was more abundant at E13–14 (P < 0.05), Vegfa_164 was less abundant at E13 (P < 0.05), and Vegfa_165b tended to be ...


Genetic Mapping Of Quantitative Trait Loci Associated With Bioenergy Traits, And The Assessment Of Genetic Variability In Sweet Sorghum (Sorghum Bicolor (L.). Moench), Aatshwaelwe Lekgari Lekgari 2010 University of Nebraska at Lincoln

Genetic Mapping Of Quantitative Trait Loci Associated With Bioenergy Traits, And The Assessment Of Genetic Variability In Sweet Sorghum (Sorghum Bicolor (L.). Moench), Aatshwaelwe Lekgari Lekgari

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Sweet sorghum, a botanical variety of sorghum is a potential source of bioenergy because high sugar levels accumulate in its stalks. The objectives of this study were to explore the global diversity of sweet sorghum germplasm, and map the genomic regions that are associated with bioenergy traits. In assessing diversity, 142 sweet sorghum accessions were evaluated with three marker types (SSR, SRAP, and morphological markers) to determine the degree of relatedness among the accessions. The traits measured (anthesis date [AD], plant height [PH], biomass yield [BY], and moisture content [MC]) were all significantly different (P<0.05) among accessions. Morphological marker clustered the accessions into five groups based on PH, MC and AD. The three traits accounted for 92.5% of the variation. There were four and five groups based on SRAP and SSR data respectively classifying accessions mainly on their origin or breeding history. The observed difference between SSR and SRAP based clusters could be attributed to the difference in marker type. SSRs amplify any region of the genome whereas SRAP amplify the open reading frames and promoter regions. Comparing the three marker-type clusters, the markers complimented each other in grouping accessions and would be valuable in assisting breeders to select appropriate lines for crossing. In evaluating QTLs that are associated with bioenergy traits, 165 recombinant inbred lines (RILs) were planted at four environments in Nebraska. A genetic linkage map constructed spanned a length of 1541.3 cM, and generated 18 linkage groups that aligned to the 10 sorghum chromosomes. Fourteen QTLs (6 for brix, 3 for BY, 2 each for AD and MC, and 1 for PH) were mapped. QTLs for the traits that were significantly correlated, colocalized in two clusters on linkage group Sbi01b. Both parents contributed beneficial alleles for most of traits measured, supporting the transgressive segregation in this population. Additional work is needed on exploiting the usefulness of chromosome 1 in breeding sorghum for bioenergy.


Xenia Effects On Quality Of Maize Female Inbred In Production Of Hybrid Seeds, Leandro Castañeda 2010 University of Nebraska at Lincoln

Xenia Effects On Quality Of Maize Female Inbred In Production Of Hybrid Seeds, Leandro Castañeda

Theses, Dissertations, and Student Research in Agronomy and Horticulture

In Maize (Zea maize L.), cost of hybrid seed production is directly related to the yield and quality of seed obtained per hectare of female parent. It is also important to consider the effects that a male parent can exert on the development of hybrid seed in the female parent. This effect is known as xenia. The objectives of this study were to evaluate xenia effects on 1) yield as 80K units, 2) germination of the hybrid seed and 3) susceptibility of the hybrid seed to mechanical damage. One female inbred and four male inbred lines were selected from a ...


Evaluation Of Health And Diabetes Knowledge Of Cu4health Participants By Food Frequency Questionnaire And Michigan Diabetes Knowledge Test, Peter Mukwevho 2010 Clemson University

Evaluation Of Health And Diabetes Knowledge Of Cu4health Participants By Food Frequency Questionnaire And Michigan Diabetes Knowledge Test, Peter Mukwevho

All Theses

Type 2 Diabetes (T2D) has been steadily rising in the United States. The condition is characterized by elevated blood glucose levels and is preceded by insulin resistance. Before the onset of T2D patients go through a state of pre-diabetes. To be tested for pre-diabetes and diabetes, either a Fasting Plasma Glucose test or an Oral Glucose Tolerance Test may be used. While an estimated 24 million Americans have T2D, another 57 million Americans are estimated to be in a state of pre-diabetes. There are many risk factors that may contribute to the development of T2D including obesity, a high calorie ...


Further Purification And Characterization Of Jenseniin P, A Bacteriocin Produced By Propionibacterium Jensenii B1264, Gaoyan Wang 2010 Clemson University

Further Purification And Characterization Of Jenseniin P, A Bacteriocin Produced By Propionibacterium Jensenii B1264, Gaoyan Wang

All Theses

Jenseniin P, a bacteriocin discovered by Prince, can inhibit the growth of several strains of propionibacteria and lactobacillus(Prince 1993). Ratnam further purified jenseniin P by ammonia sulfate precipitation and anion exchange chromatography. In that study, partially purified jenseniin P was stable in various pH ranges, SDS concentrations, several solvents, and remained active after holding at 100 ¡C for 60 minutes. The molecular weight was estimated to be between 6 and 9 kDa. In addition, jenseniin P was demonstrated to be inhibitory to Propionibacterium acnes using the spot-on-lawn method. Thus, Ratnam postulated its possible application in acne treatment(Ratnam 1997 ...


Performance And Carcass Characteristics Of Finishing Beef Cattle Managed In A Bedded Hoop-Barn System, Mark S. Honeyman, W. D. Busby, Steven M. Lonergan, Anna K. Butters-Johnson, Dallas L. Maxwell, Jay D. Harmon, Shawn C. Shouse 2010 Iowa State University

Performance And Carcass Characteristics Of Finishing Beef Cattle Managed In A Bedded Hoop-Barn System, Mark S. Honeyman, W. D. Busby, Steven M. Lonergan, Anna K. Butters-Johnson, Dallas L. Maxwell, Jay D. Harmon, Shawn C. Shouse

Animal Science Publications

The use of bedded hoop barns in finishing systems for beef cattle has not been widely researched. In this management system, beef cattle are confined to hoop barns throughout finishing, and bedding is used to absorb animal waste, which results in minimal effluent. The objective of this study was to compare the performance and carcass characteristics of finishing beef steers (n = 1,428) managed in a bedded hoop-barn management system vs. an open-feedlot system with shelter. Six feeding trials were conducted over a 3-yr period. Three trials were conducted during summer-fall and 3 trials were conducted during winter-spring. Crossbred steers ...


Effects Of Common Culinary Cooking Techniques On The Antioxidants In Collard Greens, Alexander Clifford 2010 Clemson University

Effects Of Common Culinary Cooking Techniques On The Antioxidants In Collard Greens, Alexander Clifford

All Theses

This thesis explores the effects of different cooking techniques on the antioxidant activity in collard greens. Chapter 1 is a literature review covering the topics of antioxidants, nutrition, cooking techniques and the effect of thermal treatment on antioxidants. Chapter 2 is a research manuscript evaluating collard greens from a local South Carolina farm exposed to seven different thermal treatments along with an untreated raw group. The thermal treatments utilized were sautŽ, both long and short simmer, the sautŽ treatment applied to both a long and short simmer treated group and the reserved cooking water from both a long and short ...


Effects Of An Enriched And Barren Environment On Behavioral And Physiological Responses Related To Laying Hen Welfare, Caroline Davis 2010 Clemson University

Effects Of An Enriched And Barren Environment On Behavioral And Physiological Responses Related To Laying Hen Welfare, Caroline Davis

All Theses

This study was conducted to determine the effects of housing environment on behavioral and physiological responses related to laying hen welfare. General observations and two behavioral fear assessments (emergence test [EM] and tonic immobility test [TI]) were used as behavioral assessments. A heterophil/lymphocyte ratio was used as a physiological stress indicator. Nine hundred day-old Leghorn chicks were randomly assigned to either a floor pen environment or a commercial cage housing environment. The cage chicks were housed in 20 commercial battery brooder cages (25 per cage) up until four weeks of age and then moved to 39 battery grower cages ...


Seed Removal By The Red-Rumped Agouti, Dasyprocta Leporina, On A Caribbean Island, Benton Taylor 2010 Clemson University

Seed Removal By The Red-Rumped Agouti, Dasyprocta Leporina, On A Caribbean Island, Benton Taylor

All Theses

Agoutis are important seed dispersers as well as predators of numerous plant species in Neotropical continental rainforests, but little is known about their role as seed removers (dispersers and predators) on islands. We investigated seed removal of seven rain forest species on the island of Dominica in the Lesser Antilles by the entire seed-remover community and specifically by the Red-rumped Agouti, Dasyprocta leporina, a scatterhoarding rodent introduced to the island approximately 2500 years ago. We recorded removal rates in three regions of Dominica from 168 experimentally placed seed groups containing a total of 1356 seeds. Seed groups were either accessible ...


In Memoriam: Mark Dmitrievich Sonin, 1932-2010, K. V. Galaktionov, A. J. Russ, O. Pugachev, A. Pelgunov, A. L. Kontrimavichus, Eric P. Hoberg 2010 Zoological Institute of the Russian Academy of Sciences

In Memoriam: Mark Dmitrievich Sonin, 1932-2010, K. V. Galaktionov, A. J. Russ, O. Pugachev, A. Pelgunov, A. L. Kontrimavichus, Eric P. Hoberg

Faculty Publications from the Harold W. Manter Laboratory of Parasitology

In memoriam for Mark Dmitrievich Sonin, parasitologist at the Russian Academy of Sciences, 1932-2010.


Mixing Considerations In Stirred Tank Bioreactors When Using Fluid Property Altering Microorganisms, Enes Kadic, Theodore J. Heindel 2010 Iowa State University

Mixing Considerations In Stirred Tank Bioreactors When Using Fluid Property Altering Microorganisms, Enes Kadic, Theodore J. Heindel

Mechanical Engineering Conference Presentations, Papers, and Proceedings

Stirred tank reactors are one of the standard reactors in the chemical industry and have been widely implemented for biological applications. They are used with viscous liquids, slurries, very low gas flow rates, and large liquid volumes. Stirred tank bioreactors are popular because a well-mixed state, required or preferred for numerous biological processes, is usually achieved in such situations; however, many production processes using microorganisms tend to experience fluid property alterations, which significantly impact mixing, operational parameters, and process results. The most troubling issues occur when a fluid gradually undergoes a viscosity change and/or slowly exhibits non-Newtonian behavior due ...


Pattern Space Maintenance For Data Updates And Interactive Mining, Mengling Feng, Guozhu Dong, Jinyan Li, Yap-Peng Tan, Limsoon Wong 2010 Wright State University - Main Campus

Pattern Space Maintenance For Data Updates And Interactive Mining, Mengling Feng, Guozhu Dong, Jinyan Li, Yap-Peng Tan, Limsoon Wong

Kno.e.sis Publications

This article addresses the incremental and decremental maintenance of the frequent pattern space. We conduct an in-depth investigation on how the frequent pattern space evolves under both incremental and decremental updates. Based on the evolution analysis, a new data structure, Generator-Enumeration Tree (GE-tree), is developed to facilitate the maintenance of the frequent pattern space. With the concept of GE-tree, we propose two novel algorithms, Pattern Space Maintainer+ (PSM+) and Pattern Space Maintainer− (PSM−), for the incremental and decremental maintenance of frequent patterns. Experimental results demonstrate that the proposed algorithms, on average, outperform the representative state-of-the-art methods by an order of ...


The Joint Fire Science Program’S First 10 Years, Gail Wells 2010 Gail Wells Communications

The Joint Fire Science Program’S First 10 Years, Gail Wells

JFSP Fire Science Digests

Fire scientists and managers at the 4th International Fire Ecology and Management Congress offer their thoughts about the program’s accomplishments, challenges, and future direction


Hexapod Herald- Volume 22 Number 4, August 2010, 2010 University of Nebraska - Lincoln

Hexapod Herald- Volume 22 Number 4, August 2010

Hexapod Herald & Other Entomology Department Newsletters

Welcome

Congratulations

Grants

Faculty News

Meet and Off Campus Student

Student News

From the Office

Travel

Condolences


Checklist Of The Cerambycidae (Coleoptera) Of Costa Rica, Ian P. Swift, Larry G. Bezark, Eugenio H. Nearns, Ángel Solís, Frank T. Hovore 2010 California State Collection of Arthropods

Checklist Of The Cerambycidae (Coleoptera) Of Costa Rica, Ian P. Swift, Larry G. Bezark, Eugenio H. Nearns, Ángel Solís, Frank T. Hovore

Insecta Mundi

An updated checklist of the Cerambycidae of Costa Rica is presented. This new version includes 1,071 species and subspecies in 429 genera, 69 tribes, and six subfamilies. Of these, 181 are new country records and 136 species are known only from Costa Rica. In addition, provincial distribution data are provided for each species. The checklist supports a wealth of scientific literature in many other groups of flora and fauna indicating Costa Rica has high species richness of cerambycid beetles.

Se presenta una lista actualizada de los Cerambycidae de Costa Rica. Esta nueva versión incluye 1.071 especies y subespecies ...


Scraping Behavior In Male White-Tailed Deer As A Potential Means Of Transmitting Chronic Wasting Disease, Travis C. Kinsell 2010 University of Nebraska at Lincoln

Scraping Behavior In Male White-Tailed Deer As A Potential Means Of Transmitting Chronic Wasting Disease, Travis C. Kinsell

Dissertations & Theses in Natural Resources

Chronic wasting disease (CWD) has become a concern for wildlife managers and hunters across the United States. High prevalence of chronic wasting disease (CWD) in older male white-tailed deer (Odocoileus virginianus) suggests that sex-specific social behavior may contribute to the spread of the disease among males. Scraping is a marking behavior performed by male white-tailed deer during the rut in which a pawed depression and associated over-hanging branch are marked with saliva, glandular secretions, urine, and feces. We placed 71 and 35 motion-activated cameras on scrapes in DeSoto National Wildlife Refuge in western Nebraska and eastern Iowa from Oct. – Nov ...


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