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Circadian Wheel-Running Activity During Withdrawal From Chronic Intermittent Ethanol Exposure In Mice, Joseph Seggio 2009 Bridgewater State College

Circadian Wheel-Running Activity During Withdrawal From Chronic Intermittent Ethanol Exposure In Mice, Joseph Seggio

Joseph A. Seggio

Alcohol withdrawal is associated with affective–behavioral disturbances in both human alcoholics and in animal models. In general, these phenomena are potentiated by increased alcohol exposure duration and by prior withdrawal episodes. Previous studies have also reported locomotor hypoactivity during ethanol withdrawal in rats and mice, but only in novel test environments and not in the home cage. In the present study, we examined the effects of withdrawal from chronic intermittent ethanol (CIE) vapor exposure on the level and circadian periodicity of wheel-running activity in C57BL/6J mice. CIE treatment resulted in reductions in wheel-running activity compared with plain-air controls ...


Using A Free Online Citizen-Science Project To Teach Observation & Quantification Of Animal Behavior, Margaret Voss, Caren Cooper 2009 No institutional affiliation

Using A Free Online Citizen-Science Project To Teach Observation & Quantification Of Animal Behavior, Margaret Voss, Caren Cooper

Margaret A Voss

Students of animal behavior must learn to accurately define and quantify observations and to be aware of observational bias. We designed an introductory, college-level animal behavior laboratory that uses a free, online citizen-science project (CamClickr) to help students identify and overcome biases when interpreting observations. The exercise encourages proficiency in making structural descriptions of animal behavior while teaching students to use ethograms to generate and test discrete hypotheses.


The Genomics Education Partnership: Successful Integration Of Research Into Laboratory Classes At A Diverse Group Of Undergraduate Institutions, Paul Overvoorde, Chris Shaffer, Consuelo Alvarez, Cheryl Bailey, Daron Barnard, Satish Bhalla, Chitra Chandrasekaran, Vidya Chandrasekaran, Hui-Min Chung, Doug Dorer, Chunguang Du, Todd Eckdahl, Jeff Poet, Donald Frohlich, Anya Goodman, Yuying Gosser, Charles Hauser, Laura Hoopes, Diana Johnson, Chris Jones, Marian Kaehler, Nighat Kokan, Olga Kopp, Gary Kuleck, Gerry McNeil, Robert Moss, Jennifer Myka, Alexis Nagengast, Robert Morris, Libby Shoop, Susan Parrish, Kelly Reed, E. Gloria Regisford, Dennis Revie, Anne Rosenwald, Ken Saville, Stephanie Schroeder, Mary Shaw, Christopher Smith, Mary Smith, Eric Spana, Mary Spratt, Joyce Stamm, Jeff Thompson, Matthew Wawersik, Barbara Wilson, Jim Youngblom, Wilson Leung, David Lopatto, Sarah Elgin 2009 Macalester College

The Genomics Education Partnership: Successful Integration Of Research Into Laboratory Classes At A Diverse Group Of Undergraduate Institutions, Paul Overvoorde, Chris Shaffer, Consuelo Alvarez, Cheryl Bailey, Daron Barnard, Satish Bhalla, Chitra Chandrasekaran, Vidya Chandrasekaran, Hui-Min Chung, Doug Dorer, Chunguang Du, Todd Eckdahl, Jeff Poet, Donald Frohlich, Anya Goodman, Yuying Gosser, Charles Hauser, Laura Hoopes, Diana Johnson, Chris Jones, Marian Kaehler, Nighat Kokan, Olga Kopp, Gary Kuleck, Gerry Mcneil, Robert Moss, Jennifer Myka, Alexis Nagengast, Robert Morris, Libby Shoop, Susan Parrish, Kelly Reed, E. Gloria Regisford, Dennis Revie, Anne Rosenwald, Ken Saville, Stephanie Schroeder, Mary Shaw, Christopher Smith, Mary Smith, Eric Spana, Mary Spratt, Joyce Stamm, Jeff Thompson, Matthew Wawersik, Barbara Wilson, Jim Youngblom, Wilson Leung, David Lopatto, Sarah Elgin

Paul Overvoorde

No abstract provided.


Composition Of Ceanothus Gregii Oil As Determined By Steam Distillation And Solid-Phase Microextraction, Ed Frederickson, R. Estell, M. Lucero 2009 Eastern Kentucky University

Composition Of Ceanothus Gregii Oil As Determined By Steam Distillation And Solid-Phase Microextraction, Ed Frederickson, R. Estell, M. Lucero

Ed L. Frederickson

Ceanothus gregii Gray was collected from the Jornada Experimental Range in south central New Mexico. Current year’s growth was collected from ten plants found within an approximate 50 m radius of the GPS coordinates N32°40.605’ and W106°33.486’at an altitude of 1,741 m during July 2001. Composite samples of the plants were steam distilled in triplicate, and the composite oil was analyzed using both GC-FID and GC/MS. The volatile composition of the same plants was also examined using solid-phase microextraction (SPME) with a 100?m polydimethylsiloxane fiber. Mass spectra and retention indices were ...


Bees In America: How The Honey Bee Shaped A Nation, Tammy Horn 2009 Eastern Kentucky University

Bees In America: How The Honey Bee Shaped A Nation, Tammy Horn

Tammy Horn

" Honey bees--and the qualities associated with them--have quietly influenced American values for four centuries. During every major period in the country's history, bees and beekeepers have represented order and stability in a country without a national religion, political party, or language. Bees in America is an enlightening cultural history of bees and beekeeping in the United States. Tammy Horn, herself a beekeeper, offers a varied social and technological history from the colonial period, when the British first introduced bees to the New World, to the present, when bees are being used by the American military to detect bombs. Early ...


Characterizing Weed Populations In Different Turfgrass Sites Throughout The Klang Valley Of Western Peninsular Malaysia, Md Kamal Uddin, Abdul Juraimi, Mohd Ismail, Jim Brosnan 2009 University of Tennessee, Knoxville

Characterizing Weed Populations In Different Turfgrass Sites Throughout The Klang Valley Of Western Peninsular Malaysia, Md Kamal Uddin, Abdul Juraimi, Mohd Ismail, Jim Brosnan

Jim Brosnan

A field survey was conducted in 2007 to characterize weed populations in different turfgrass sites throughout the Klang Valley of western Peninsular Malaysia. Sites included golf course putting greens, athletic fields, sod farms, and residential lawns. Weeds present in each site were identified and the data were used to calculate frequency, distribution uniformity, density, relative abundance, and community coefficient values for each species. Seventy-nine weed species, belonging to 16 families, were found. The most species were found on residential lawns, and the fewest were found on golf course putting greens; athletic fields and sod farms ranked intermediately. A total of ...


Review Of Pregnancy Diagnosis Techniques In Cattle And Small Ruminants, Brian Whitlock, Elizabeth Coffman 2009 University of Tennessee - Knoxville

Review Of Pregnancy Diagnosis Techniques In Cattle And Small Ruminants, Brian Whitlock, Elizabeth Coffman

Brian K Whitlock, PhD, DVM, DACT

Pregnancy diagnosis is a common management practice for ruminants and there are a number of methods available, with each having advantage(s) and/or disadvantage(s). A brief review of pregnancy diagnosis techniques in cattle, goats, and sheep is provided.


Epizootic Ulcerative Syndrome Caused By Aphanomyces Invadans In Captive Bullseye Snakehead Channa Marulius Collected From South Florida, Usa., R. Saylor, Debra Miller, M. Vandersea, M. Bevelhimer, P. Schofield, W. Bennett 2009 University of Tennessee, Knoxville

Epizootic Ulcerative Syndrome Caused By Aphanomyces Invadans In Captive Bullseye Snakehead Channa Marulius Collected From South Florida, Usa., R. Saylor, Debra Miller, M. Vandersea, M. Bevelhimer, P. Schofield, W. Bennett

Debra L Miller

Epizootic ulcerative syndrome (EUS) caused by the oomycete Aphanomyces invadans is an invasive, opportunistic disease of both freshwater and estuarine fishes. Originally documented as the cause of mycotic granulomatosis of ornamental fishes in Japan and as the cause of EUS of fishes in southeast Asia and Australia, this pathogen is also present in estuaries and freshwater bodies of the Atlantic and gulf coasts of the USA. We describe a mass mortality event of 343 captive juvenile bullseye snakehead Channa marulius collected from freshwater canals in Miami-Dade County, Florida. Clinical signs appeared within the first 2 d of captivity and included ...


Functional Anatomy Of Penaeid Shrimp, Gary Martin, Jo Hose 2009 Occidental College

Functional Anatomy Of Penaeid Shrimp, Gary Martin, Jo Hose

Gary Martin

A comprehensive source of information on all aspects of shrimp production, this reference covers not only the global status of shrimp farming, but also examines shrimp anatomy and physiology. From nutrition to health management and harvesting issues to biosecurity, this well-researched volume evaluates existing knowledge, proposes new concepts, and questions common practices. With an extensive review on worldwide production systems, this compilation will be highly relevant to research scientists, students, and shrimp producers.


Pharmacology Supplement, Ahmed Mustafa, Ellioott Blumenthal 2009 Indiana University Purdue University Fort Wayne

Pharmacology Supplement, Ahmed Mustafa, Ellioott Blumenthal

Ahmed Mustafa Dr.

No abstract provided.


Response To Vaccination With A Commercial Inactivated Rabies Vaccine In A Captive Colony Of Brazilian Free-Tailed Bats (Tadarida Brasiliensis), Amy Turmelle, Louise Allen, Barbara Schmidt-French, Felix Jackson, Thomas Kunz, Charles Rupprecht, Gary McCracken 2009 University of Tennessee, Knoxville

Response To Vaccination With A Commercial Inactivated Rabies Vaccine In A Captive Colony Of Brazilian Free-Tailed Bats (Tadarida Brasiliensis), Amy Turmelle, Louise Allen, Barbara Schmidt-French, Felix Jackson, Thomas Kunz, Charles Rupprecht, Gary Mccracken

Gary F. McCracken

A captive colony of Brazilian free-tailed bats (Tadarida brasiliensis) was vaccinated with a commercial monovalent inactivated rabies virus (RABV) vaccine (RABVAC 1). Baseline rabies virus neutralizing antibodies (VNA) and the response to vaccination were measured in 50 bats. Rabies VNA was detected in the plasma of 64% (27/42) of bats that had been vaccinated 1 yr prior, but only 19% (8/42) had levels considered adequate. Rabies VNA was detected in the plasma of 63% (5/8) of bats with no record of previous vaccination, suggesting natural RABV exposure before captivity. All bats demonstrated a VNA response by 10 ...


Batrachochytrium Dendrobatidis And The Collapse Of Anuran Species Richness And Abundance In The Upper Manu National Park, Southeastern Peru, Edgar Lehr, Allessandro Catenazzi, Lily Rodriguez, Vance Vrendeburg 2009 Illinois Wesleyan University

Batrachochytrium Dendrobatidis And The Collapse Of Anuran Species Richness And Abundance In The Upper Manu National Park, Southeastern Peru, Edgar Lehr, Allessandro Catenazzi, Lily Rodriguez, Vance Vrendeburg

Edgar Lehr

Amphibians are declining worldwide, but these declines have been particularly dramatic in tropical mountains, where high endemism and vulnerability to an introduced fungal pathogen, Batrachochytrium dendrobatidis (Bd), is associated with amphibian extinctions. We surveyed frogs in the Peruvian Andes in montane forests along a steep elevational gradient (1200-3700 m). We used visual encounter surveys to sample stream-dwelling and arboreal species and leaf-litter plots to sample terrestrial-breeding species. We compared species richness and abundance among the wet seasons of 1999, 2008, and 2009. Despite similar sampling effort among years, the number of species (46 in 1999) declined by 47% between 1999 ...


Darwinian Controversies: An Historiographical Recounting, David Depew 2009 University of Iowa

Darwinian Controversies: An Historiographical Recounting, David Depew

David J Depew

This essay reviews key controversies in the history of the Darwinian research tradition: the Wilberforce-Huxley debate in 1860, early twentieth-century debates about the heritability of acquired characteristics and the consistency of Mendelian genetics with natural selection; the 1925 Scopes trial about teaching evolution; tensions about race, culture, and eugenics at the 1959 centenary celebration Darwin’s Origin of Species; adaptationism and its critics in the Sociobiology debate of 1970s and, more recently, Evolutionary Psychology; and current disputes about Intelligent Design. These controversies, I argue, are etched into public memory because they occur at the emotionally charged boundaries between public-political, technical-scientific ...


Haematopoietic Stem Cells In Spleen Have Distinct Differentiative Potential For Antigen Presenting Cells., Jonathan Tan, Helen O'Neill 2009 Australian National University

Haematopoietic Stem Cells In Spleen Have Distinct Differentiative Potential For Antigen Presenting Cells., Jonathan Tan, Helen O'Neill

Helen O'Neill

Dendritic cells (DC) are known to develop from macrophage dendritic progenitors (MDP) in bone marrow (BM), which give rise to conventional (c)DC and monocytes, both dominant antigen presenting cell (APC) subsets in spleen. This laboratory has however defined a distinct dendritic-like cell subset in spleen (L-DC), which can also be derived in long-term cultures of spleen. In line with the restricted in vitro development of only L-DC in these stromal cultures, we questioned whether self-renewing HSC or progenitors exist in spleen with restricted differentiative capacity for only L-DC. Neonatal spleen and BM were compared for their ability to reconstitute ...


“Inheritance, Race And The Four Major Factors For The Unity Between African Americans And European Americans: Land/Territory, Blood/Genes, Religion And Language,”, Amadu Kaba 2009 Seton Hall University

“Inheritance, Race And The Four Major Factors For The Unity Between African Americans And European Americans: Land/Territory, Blood/Genes, Religion And Language,”, Amadu Kaba

Amadu Jacky Kaba

There is no American or human being in the United States or the world today alive 150 years ago. This means that American citizens today have inherited one of the richest territories in the world from their ancestors who inhabited the land before them. No other people have inherited such a wealthy and geographically diverse territory. In 1990, for example, the buying power of all Americans was $4.27 trillion. By 2009, that figure increased to $10.72 trillion. Apart from Native Americans, people of Black African descent and people of European descent are the oldest inhabitants of the United ...


Gingival Osteogenic Melanoma In Two Dogs., A. Ellis, B. Harmon, Debra Miller, N. Northup, K. Latimer, E. Uhl 2009 University of Tennessee, Knoxville

Gingival Osteogenic Melanoma In Two Dogs., A. Ellis, B. Harmon, Debra Miller, N. Northup, K. Latimer, E. Uhl

Debra L Miller

Osteogenic melanoma is a rare variant of metaplastic malignant melanoma in human medicine and appears to be a similarly rare variant in dogs. Two dogs with oral malignant melanoma with neoplastic bone formation are reported in this study. Both tumors were characterized by malignant melanocytes that transitioned into neoplastic bone at the deep margins of the neoplasm. Immunohistochemical analysis revealed S100- and Melan-A-positive neoplastic cells adjacent to, and occasionally embedded within, an osteoid and chondroblastic matrix. Scattered clusters of neoplastic cells were also positive for osteocalcin. The findings indicate that in dogs, as in humans, neoplastic melanocytes have metaplastic potential ...


Expression Profiling Of Crambe Abyssinica Under Arsenate Stress Identifies Genes And Gene Networks Involved In Arsenic Metabolism And Detoxification, Om Parkash, Suganthi Kandasamy, Bibin Paulose 2009 University of Massachusetts - Amherst

Expression Profiling Of Crambe Abyssinica Under Arsenate Stress Identifies Genes And Gene Networks Involved In Arsenic Metabolism And Detoxification, Om Parkash, Suganthi Kandasamy, Bibin Paulose

Om Parkash

Background Arsenic contamination is widespread throughout the world and this toxic metalloid is known to cause cancers of organs such as liver, kidney, skin, and lung in human. In spite of a recent surge in arsenic related studies, we are still far from a comprehensive understanding of arsenic uptake, detoxification, and sequestration in plants. Crambe abyssinica, commonly known as 'abyssinian mustard', is a non-food, high biomass oil seed crop that is naturally tolerant to heavy metals. Moreover, it accumulates significantly higher levels of arsenic as compared to other species of the Brassicaceae family. Thus, C. abyssinica has great potential to ...


Lysophosphatidic Acid Induction Of Interleukin 6 Secretion From Aortic Smooth Muscle Cells Via Lpa1 Regulated, Pkc Dependent And P38alpha Mediated Pathway, F. Hao, M. Tan, D. Wu, X. Xu, Mei Cui 2009 University of Tennessee, Knoxville

Lysophosphatidic Acid Induction Of Interleukin 6 Secretion From Aortic Smooth Muscle Cells Via Lpa1 Regulated, Pkc Dependent And P38alpha Mediated Pathway, F. Hao, M. Tan, D. Wu, X. Xu, Mei Cui

Mei-Zhen Cui

Lysophosphatidic acid (LPA) is a potent bioactive lysophospholipid. Accumulated evidence supports a role for LPA in inflammation. To profile LPA-induced cytokine production in vascular smooth muscle cells (SMCs), we used a cytokine antibody array system and found that LPA prominently induces the secretion of IL-6 and monocyte chemoattractant protein (MCP)-1 from human aortic SMCs (HASMCs). The mechanism by which LPA induces MCP-1 expression in SMCs has been previously reported. However, LPA induction of IL-6 secretion from vascular SMCs and its regulatory mechanism are unknown. The present study reveals that LPA induces the expression of IL-6 mRNA and protein in ...


Nsaid-Activated Gene-1 As A Molecular Target For Capsaicin-Induced Apoptosis Through A Novel Molecular Mechanism Involving Gsk3beta, C/Ebpbeta And Atf3, Seung Baek, Seong-Ho Lee, Chutwadee Krisanapun 2009 The University of Tennessee

Nsaid-Activated Gene-1 As A Molecular Target For Capsaicin-Induced Apoptosis Through A Novel Molecular Mechanism Involving Gsk3beta, C/Ebpbeta And Atf3, Seung Baek, Seong-Ho Lee, Chutwadee Krisanapun

Seung J Baek

Capsaicin, a natural product of the Capsicum species of red peppers, is known to induce apoptosis and suppress growth. Non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) is a cytokine associated with pro-apoptotic and antitumorigenic property in colorectal and lung cancer. Our data demonstrate that capsaicin leads to induction of apoptosis and up-regulates NAG-1 gene expression at the transcriptional level. Overexpression of CCAAT/enhancer binding protein beta (C/EBPbeta) caused a significant increase of basal and capsaicin-induced NAG-1 promoter activity. We subsequently identified C/EBPbeta binding sites in the NAG-1 promoter responsible for capsaicin-induced NAG-1 transactivation. Electrophoretic mobility shift assay and chromatin immunoprecipitation ...


Host Immunity To Repeated Rabies Virus Infection In Big Brown Bats, Gary McCracken 2009 University of Tennessee, Knoxville

Host Immunity To Repeated Rabies Virus Infection In Big Brown Bats, Gary Mccracken

Gary F. McCracken

Bats are natural reservoirs for the majority of lyssaviruses globally, and are unique among mammals in having exceptional sociality and longevity. Given these facets, and the recognized status of bats as reservoirs for rabies viruses (RABVs) in the Americas, individual bats may experience repeated exposure to RABV during their lifetime. Nevertheless, little information exists with regard to within-host infection dynamics and the role of immunological memory that may result from abortive RABV infection in bats. In this study, a cohort of big brown bats (Eptesicus fuscus) was infected intramuscularly in the left and right masseter muscles with varying doses [10 ...


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