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Nmr Assignments Of A Stable Processing Intermediate Of Human Frataxin, Kalyan C. Kondapalli, Krisztina Z. Bencze, Eric Dizin, James A. Cowan, Timothy L. Stemmler 2010 Wayne State University

Nmr Assignments Of A Stable Processing Intermediate Of Human Frataxin, Kalyan C. Kondapalli, Krisztina Z. Bencze, Eric Dizin, James A. Cowan, Timothy L. Stemmler

Biochemistry and Molecular Biology Faculty Publications

Frataxin, a nuclear encoded protein targeted to the mitochondrial matrix, has recently been implicated as an iron chaperone that delivers ferrous iron to the iron-sulfur assembly enzyme IscU. During transport across the mitochondrial membrane, the N-terminal mitochondrial targeting sequence of frataxin is cleaved in a two-step process to produce the mature protein found in the matrix, however N-terminal extended forms of the protein have also been observed in vivo. The recent structural characterization studies of the human frataxin ortholog were performed on a truncated variant of the protein. Here we report the NMR spectral assignment of an extended form of ...


Gender And Species Use In Amazonian Home Gardens: The Social And Economic Context Of Biodiversity Conservation, Leigh Ann West, David S. Salisbury, Ana I. Ríos-Sanchez, Jorge Vela Alvarado 2010 University of Richmond

Gender And Species Use In Amazonian Home Gardens: The Social And Economic Context Of Biodiversity Conservation, Leigh Ann West, David S. Salisbury, Ana I. Ríos-Sanchez, Jorge Vela Alvarado

Geography and the Environment Poster Presentations

Home gardens, “the peridomestic area belonging to the household where members plant and/or tend useful plants” (Perrault-Archambault and Coomes 2008), are found throughout the world. However, their use and importance vary from region to region. In the Peruvian Amazon, owners use home gardens for a domestic supply of foods, craft materials, medicines, condiments, and shade (Miller and Nair 2006). With this wide range in function, reflected in species content, home gardens are very biodiverse.

Home garden biodiversity may be increasingly important in a rapidly changing Amazonia (Betts et al. 2008). Thus, the sociocultural and economic factors contributing to home ...


Partitioning Of Food Resources In Bathypelagic Micronekton In The Eastern Gulf Of Mexico, Scott E. Burghart, Thomas L. Hopkins, Joseph J. Torres 2010 University of South Florida St. Petersburg

Partitioning Of Food Resources In Bathypelagic Micronekton In The Eastern Gulf Of Mexico, Scott E. Burghart, Thomas L. Hopkins, Joseph J. Torres

Marine Science Faculty Publications

A mid-water trawl survey conducted between depths of 1000 and 3000 m in the eastern Gulf of Mexico found 54% of the organisms collected were gonostomatid fishes. Lophogastrids within the family Eucopiidae were also common (17.3%), as were decapod shrimps within the families Oplophoridae and Benthesicymidae (9.9 and 7.1% respectively). We examined the gut contents of 14 prominent species from this assemblage to determine if available food resources were partitioned. Cluster analysis of stomach contents resulted in 4 clusters based on taxonomic composition and 3 based on prey size. Bristlemouth fishes (Gonostomatidae) of the genus Cyclothone and ...


Development And Evaluation Of Disease Activity Measures In Rheumatoid Arthritis Using Multi-Level Mixed Modeling And Other Statistical Methodologies: A Dissertation, Mary Jane Bentley 2010 University of Massachusetts Medical School Worcester

Development And Evaluation Of Disease Activity Measures In Rheumatoid Arthritis Using Multi-Level Mixed Modeling And Other Statistical Methodologies: A Dissertation, Mary Jane Bentley

GSBS Dissertations and Theses

Remarkable progress has been made in the development of effective treatments for patients with rheumatoid arthritis (RA). To ensure that a patient is optimally responding to treatment, consistent monitoring of disease activity is recommended. Established composite and individual disease activity measures often cannot be computed due to missing laboratory values. Simplified measures that can be calculated without a lab value have been developed and previous studies have validated these new measures, yet differences in their performance compared with established measures remain. Therefore, the goal of my doctoral research was to examine and evaluate disease activity and composite measures to facilitate ...


Effects Of Short-Term Exercise And Energy Surplus On Hormones Related To Regulation Of Energy Balance, Todd A. Hagobian, Carrie G. Sharoff, Barry Braun 2010 University of Massachusetts - Amherst

Effects Of Short-Term Exercise And Energy Surplus On Hormones Related To Regulation Of Energy Balance, Todd A. Hagobian, Carrie G. Sharoff, Barry Braun

Todd A. Hagobian

Energy surplus raises circulating concentrations of leptin and insulin while lowering plasma ghrelin. Exercise has the opposite effects. The purpose of this study was to determine whether exercise counters the hormonal effects of energy surplus independent of changes in energy balance. To do that, we assessed plasma concentrations of leptin, insulin, and ghrelin at baseline, after overfeeding, and after overfeeding plus exercise. Baseline (B) leptin and insulin concentrations and ghrelin area under the curve were measured during an oral glucose challenge in 9 healthy, active subjects (6 male, 3 female) after 2 days in energy balance without exercise. Measurements were ...


Ape1- And Ape2-Dependent Dna Breaks In Immunoglobulin Class Switch Recombination, Jeroen E. J. Guikema, Erin K. Linehan, Daisuke Tsuchimoto, Yusaku Nakabeppu, Phyllis R. Strauss, Janet Stavnezer, Carol E. Schrader 2010 University of Massachusetts Medical School

Ape1- And Ape2-Dependent Dna Breaks In Immunoglobulin Class Switch Recombination, Jeroen E. J. Guikema, Erin K. Linehan, Daisuke Tsuchimoto, Yusaku Nakabeppu, Phyllis R. Strauss, Janet Stavnezer, Carol E. Schrader

Janet M. Stavnezer

Antibody class switch recombination (CSR) occurs by an intrachromosomal deletion requiring generation of double-stranded breaks (DSBs) in switch-region DNA. The initial steps in DSB formation have been elucidated, involving cytosine deamination by activation-induced cytidine deaminase and generation of abasic sites by uracil DNA glycosylase. However, it is not known how abasic sites are converted into single-stranded breaks and, subsequently, DSBs. Apurinic/apyrimidinic endonuclease (APE) efficiently nicks DNA at abasic sites, but it is unknown whether APE participates in CSR. We address the roles of the two major mammalian APEs, APE1 and APE2, in CSR. APE1 deficiency causes embryonic lethality in ...


Supervillin Slows Cell Spreading By Facilitating Myosin Ii Activation At The Cell Periphery, Norio Takizawa, Reiko Ikebe, Mitsuo Ikebe, Elizabeth J. Luna 2010 University of Massachusetts Medical School

Supervillin Slows Cell Spreading By Facilitating Myosin Ii Activation At The Cell Periphery, Norio Takizawa, Reiko Ikebe, Mitsuo Ikebe, Elizabeth J. Luna

Elizabeth J. Luna

During cell migration, myosin II modulates adhesion, cell protrusion and actin organization at the leading edge. We show that an F-actin- and membrane-associated scaffolding protein, called supervillin (SV, p205), binds directly to the subfragment 2 domains of nonmuscle myosin IIA and myosin IIB and to the N-terminus of the long form of myosin light chain kinase (L-MLCK). SV inhibits cell spreading via an MLCK- and myosin II-dependent mechanism. Overexpression of SV reduces the rate of cell spreading, and RNAi-mediated knockdown of endogenous SV increases it. Endogenous and EGFP-tagged SV colocalize with, and enhance the formation of, cortical bundles of F-actin ...


Supervillin Reorganizes The Actin Cytoskeleton And Increases Invadopodial Efficiency, Jessica Lynn Crowley, Tara C. Smith, Zhiyou Fang, Norio Takizawa, Elizabeth J. Luna 2010 University of Massachusetts Medical School

Supervillin Reorganizes The Actin Cytoskeleton And Increases Invadopodial Efficiency, Jessica Lynn Crowley, Tara C. Smith, Zhiyou Fang, Norio Takizawa, Elizabeth J. Luna

Elizabeth J. Luna

Tumor cells use actin-rich protrusions called invadopodia to degrade extracellular matrix (ECM) and invade tissues; related structures, termed podosomes, are sites of dynamic ECM interaction. We show here that supervillin (SV), a peripheral membrane protein that binds F-actin and myosin II, reorganizes the actin cytoskeleton and potentiates invadopodial function. Overexpressed SV induces redistribution of lamellipodial cortactin and lamellipodin/RAPH1/PREL1 away from the cell periphery to internal sites and concomitantly increases the numbers of F-actin punctae. Most punctae are highly dynamic and colocalize with the podosome/invadopodial proteins, cortactin, Tks5, and cdc42. Cortactin binds SV sequences in vitro and contributes ...


Design And Methods For A Randomized Clinical Trial Treating Comorbid Obesity And Major Depressive Disorder, Kristin L. Schneider, Jamie S. Bodenlos, Yunsheng Ma, Barbara C. Olendzki, Jessica Oleski, Philip A. Merriam, Sybil L. Crawford, Ira S. Ockene, Sherry L. Pagoto 2010 University of Massachusetts Medical School

Design And Methods For A Randomized Clinical Trial Treating Comorbid Obesity And Major Depressive Disorder, Kristin L. Schneider, Jamie S. Bodenlos, Yunsheng Ma, Barbara C. Olendzki, Jessica Oleski, Philip A. Merriam, Sybil L. Crawford, Ira S. Ockene, Sherry L. Pagoto

Sybil L. Crawford

BACKGROUND: Obesity is often comorbid with depression and individuals with this comorbidity fare worse in behavioral weight loss treatment. Treating depression directly prior to behavioral weight loss treatment might bolster weight loss outcomes in this population, but this has not yet been tested in a randomized clinical trial. METHODS AND DESIGN: This randomized clinical trial will examine whether behavior therapy for depression administered prior to standard weight loss treatment produces greater weight loss than standard weight loss treatment alone. Obese women with major depressive disorder (N = 174) will be recruited from primary care clinics and the community and randomly assigned ...


Commercial Pesticide Applicator Test Help Sessions, James A. Fawcett 2010 Iowa State University

Commercial Pesticide Applicator Test Help Sessions, James A. Fawcett

Integrated Crop Management News

All commercial pesticide applicators must take exams in order to become initially certified and some choose to become re-certified by exam rather than by attending continuing instructional courses. With the arrival of Asian soybean rust in this country, many commercial applicators are considering adding agricultural diseases (category 1C) to their certification so that they can apply fungicides.


The Identification Of Rana Ocellata Linnaeus, 1758. Nomenclatural Impact On The Species Currently Known As Leptodactylus Ocellatus (Leptodactylidae) And Osteopilus Brunneus (Gosse, 1851) (Hylidae), Esteban O. Lavilla, José A. Langone, Ulisses Caramaschi, W. Ronald Heyer, Rafael O. de Sá 2010 University of Richmond

The Identification Of Rana Ocellata Linnaeus, 1758. Nomenclatural Impact On The Species Currently Known As Leptodactylus Ocellatus (Leptodactylidae) And Osteopilus Brunneus (Gosse, 1851) (Hylidae), Esteban O. Lavilla, José A. Langone, Ulisses Caramaschi, W. Ronald Heyer, Rafael O. De Sá

Biology Faculty Publications

The usage of the name Rana ocellata Linnaeus, 1758, is plagued by misidentifications and misinterpretations. After an exhaustive bibliographic review, we conclude that the Linnaeus 1758 name refers to Osteopilus brunneus (Gosse, 1851) from Jamaica, while the taxon currently known as Leptodactylus ocellatus corresponds to Rana latrans Steffen, 1815. Herein, we designate and provide descriptions of the neotypes of Leptodactylus latrans (Steffen, 1815), revalidated, new combination, and Osteopilus ocellatus (Linnaeus, 1758), new combination, as a senior synonym of Osteopilus brunneus (Gosse, 1851).


Estrogen Receptor-Alpha In The Bed Nucleus Of The Stria Terminalis Regulates Social Affiliation In Male Prairie Voles (Microtus Ochrogaster), Kelly Lei, Bruce S. Cushing, Sergei Musatov, Sonoko Ogawa, Kristin M. Kramer 2010 University of Memphis

Estrogen Receptor-Alpha In The Bed Nucleus Of The Stria Terminalis Regulates Social Affiliation In Male Prairie Voles (Microtus Ochrogaster), Kelly Lei, Bruce S. Cushing, Sergei Musatov, Sonoko Ogawa, Kristin M. Kramer

Biology Faculty Research

Estrogen receptor alpha (ER alpha) typically masculinizes male behavior, while low levels of ER alpha in the medial amygdala (MeA) and the bed nucleus of the stria terminalis (BST) are associated with high levels of male prosocial behavior. In the males of the highly social prairie vole (Microtus ochrogaster), increasing ER alpha in the MeA inhibited the expression of spontaneous alloparental behavior and produced a preference for novel females. To test for the effects of increased ER alpha in the BST, a viral vector was used to enhance ER alpha expression in the BST of adult male prairie voles. Following ...


Evaluating The Skeletal Chemistry Of Mytilus Californianus As A Temperature Proxy: Effects Of Microenvironment And Ontogeny, Heather L. Ford, Stephen A. Schellenberg, Bonnie Becker, Douglas L. Deutschman, Kelsey A. Dyck, Paul L. Koch 2010 University of California - Santa Cruz

Evaluating The Skeletal Chemistry Of Mytilus Californianus As A Temperature Proxy: Effects Of Microenvironment And Ontogeny, Heather L. Ford, Stephen A. Schellenberg, Bonnie Becker, Douglas L. Deutschman, Kelsey A. Dyck, Paul L. Koch

SIAS Faculty Publications

Molluscan shell chemistry may provide an important archive of mean annual temperature (MAT) and mean annual range in temperature (MART), but such direct temperature interpretations may be confounded by biologic, metabolic, or kinetic factors. To explore this potential archive, we outplanted variously sized specimens of the common mussel Mytilus californianus at relatively low and high intertidal positions in San Diego, California, for 382 days with in situ recording of ambient temperature and periodic sampling of water chemistry. The prismatic calcite layer of eight variously sized specimens from each intertidal position were then serially microsampled and geochemically analyzed. Average intraspecimen delta ...


Constitutively Overexpressed 21 Kda Protein In Hodgkin Lymphoma And Aggressive Non-Hodgkin Lymphomas Identified As Cytochrome B5b (Cyb5b)., Derek Murphy, Jeremy Parker, Minglong Zhou, Faisal M. Fadlelmola, Christian Steidl, Aly Karsan, Randy D. Gascoyne, Hong Chen, Diponkar Banerjee 2010 Royal College of Surgeons in Ireland

Constitutively Overexpressed 21 Kda Protein In Hodgkin Lymphoma And Aggressive Non-Hodgkin Lymphomas Identified As Cytochrome B5b (Cyb5b)., Derek Murphy, Jeremy Parker, Minglong Zhou, Faisal M. Fadlelmola, Christian Steidl, Aly Karsan, Randy D. Gascoyne, Hong Chen, Diponkar Banerjee

Centre for Human Proteomics Articles

BACKGROUND: We have previously reported a novel constitutively overexpressed 21 kDa protein in Hodgkin Lymphoma (HL) and aggressive Non-Hodgkin Lymphomas (NHL). The objective of the current study was to 1) identify this protein using two independent methods, 2) study the expression of the protein and its encoding mRNA in reactive lymph nodes, normal lymphocytes and CD34+ bone marrow precursor cells, 3) analyse patterns of expression of the protein in tissue microarrays assembled from a large number of diagnostic clinical biopsies from patients with HL, and 4) determine the copy number variation and mutation status of the encoding gene in HL ...


Stress Hyperactivation In The Beta-Cell, Sonya G. Fonseca, Fumihiko Urano, Mark Burcin, Jesper Gromada 2010 Novartis Institutes for Biomedical Research

Stress Hyperactivation In The Beta-Cell, Sonya G. Fonseca, Fumihiko Urano, Mark Burcin, Jesper Gromada

Program in Gene Function and Expression Publications and Presentations

In pancreatic beta-cells, the endoplasmic reticulum (ER) is the crucial site for insulin biosynthesis, as this is where the protein-folding machinery for secretory proteins is localized. Perturbations to ER function of the beta-cell, such as a high demand for insulin secretion, can lead to an imbalance in protein homeostasis and lead to ER stress. This stress can be mitigated by an adaptive, cellular response, the unfolded protein response (UPR). UPR activation is vital to the survival of beta-cells, as these cells represent one of the most susceptible tissues for ER stress, due to their highly secretory function. However, in some ...


Elephants - A Conservation Overview, Heidi S. Riddle, Bruce A. Schulte, Ajay A. Desai, Laura van der Meer 2010 Western Kentucky University

Elephants - A Conservation Overview, Heidi S. Riddle, Bruce A. Schulte, Ajay A. Desai, Laura Van Der Meer

Bruce A. Schulte

Loss of habitat is one of the most significant problems facing elephants worldwide,
leading to clashes over resources between wildlife and humans where elephants receive the
largest part of blame – defined as Human Elephant Conflict (HEC). The sub-Saharan region of
Africa contains an approximate population of 500,000 elephants that occupy 37 range countries.
The African Elephant (Loxodonta africana) is categorized as Vulnerable in the Red List of
Threatened Species; they are listed there as two distinct subspecies: the Savanna Elephant (L.
a. africana) and the Forest Elephant (L. a. cyclotis). The Red List of Threatened Species
categorizes the Asian ...


Crop Scout School March 6, Brent A. Pringnitz 2010 Iowa State University

Crop Scout School March 6, Brent A. Pringnitz

Integrated Crop Management News

If you plan to work as a crop scout for the 2010 crop season, you will want to attend the upcoming Crop Scout School offered by Iowa State University Extension. Hands-on sessions will cover identification and scouting methods for weeds, insects and diseases. Workshops on plant growth and development of corn, soybean and alfalfa will be included along with discussion of common field crop problems.


Soil Fertility Short Course Feb. 9-10, Brent A. Pringnitz 2010 Iowa State University

Soil Fertility Short Course Feb. 9-10, Brent A. Pringnitz

Integrated Crop Management News

Understanding crop nutrient needs and soil fertility issues is vital to profitable crop production. ISU Extension is offering a two-day short course focusing on principles of soils, soil fertility and nutrient management to help crop production professionals make better decisions. In addition to classroom work, attendees will also be visiting the ISU Soil and Plant Analysis Laboratory.


Study Of Secondary Metabolite Gene Expression In Marine Microbial Co-Cultures Using Quantitative Real-Time Pcr, Crystal A. Conway 2010 Nova Southeastern University

Study Of Secondary Metabolite Gene Expression In Marine Microbial Co-Cultures Using Quantitative Real-Time Pcr, Crystal A. Conway

HCNSO Student Theses and Dissertations

Interactions among microbial organisms often cannot be observed directly, but they can be inferred genetically using new molecular techniques. The analysis of secondary metabolite gene expression produced by co-cultured marine microbial species allows us to see how these organisms interact with one another when kept in the same environment. Co-cultures of three different strains of marine bacteria, P. aeruginosa PAO1, Roseobacter denitrificans OCH114, and Salinispora arenicola CNS-205 were grown in a laboratory setting, and using the Real-Time qPCR method gene expression levels of two different secondary metabolite producing genes from each organism was accessed across three time points. P. aeruginosa ...


Manifest Greatness The Final Original Version By Emmanuel Mario B Santos Aka Marc Guerrero, Emmanuel Mario B. Santos aka Marc Guerrero 2010 theINSTITUTE of Health & Wellness PHILIPPINES Foundation Inc

Manifest Greatness The Final Original Version By Emmanuel Mario B Santos Aka Marc Guerrero, Emmanuel Mario B. Santos Aka Marc Guerrero

Emmanuel Mario B Santos aka Marc Guerrero

MANIFEST GREATNESS vf24jan2010 WE COME TOGETHER THERE OUGHT TO BE NO POOR WE TAKE CHARGE.


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