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Plants, Processes, Places: Sensory Intimacy And Poetic Enquiry, John Ryan 2011 Edith Cowan University

Plants, Processes, Places: Sensory Intimacy And Poetic Enquiry, John Ryan

ECU Publications 2011

As an arts-based research approach, poetic enquiry has been theorised and applied recently in the social sciences and in education. In this article, I extend its usage to eco-critical studies of Australian flora and fauna. The Southwest corner of Western Australia affords opportunities to deploy arts-based methodologies, including field poetry, for celebrating the natural heritage of a region of distinguished biodiversity. I suggest that lyric practices in places such as Lesueur National Park and Anstey-Keane Damplands in southern Perth can catalyse embodied engagements with flora. The outcome of these practices is the invocation of the multiple senses— including the proximities ...


Maize Transgenes Containing Zein Promoters Are Regulated By Opaque2, Megan N. Schroder, Brice E. Floyd, M. Paul Scott 2011 Iowa State University

Maize Transgenes Containing Zein Promoters Are Regulated By Opaque2, Megan N. Schroder, Brice E. Floyd, M. Paul Scott

Agronomy Publications

Understanding epistatic interactions between transgenes and native genes is critical for predicting the response of transgenes to different genetic backgrounds and environments. Our objective was to determine if predicted epistatic interactions occur between maize (Zea mays L.) transgenes carrying zein promoters and opaque2, a transcriptional regulator of zein genes. Expression of the transgenes was significantly decreased in kernels containing the opaque2 mutation. Native zein proteins in these kernels were reduced as well. Thus, transgene expression mirrored expression of the native gene that contributed the promoter of the transgene. This work demonstrates that information about native gene expression may be useful ...


Phosphodiesterase-5 Inhibition: A Novel Strategy To Improve Stem Cell Therapy In The Heart, Nicholas Hoke 2011 Virginia Commonwealth University

Phosphodiesterase-5 Inhibition: A Novel Strategy To Improve Stem Cell Therapy In The Heart, Nicholas Hoke

Theses and Dissertations

Several studies have shown cellular replacement therapy as a treatment strategy of myocardial infarction but results have been limited. Therefore, enhancing the therapeutic potential of stem cells injected into ischemic microenvironments by novel preconditioning (PC) techniques is critical for improving cellular therapy. Recent studies have shown that inhibition of phosphodiesterase-5 (PDE-5) is a powerful strategy to precondition the heart and cardiomyocytes against ischemia/reperfusion injury. We therefore tested the hypothesis that inhibition of PDE-5 with sildenafil (Viagra®) or selective knockdown with a silencing vector in adipose derived stem cells (ASCs) would improve their survival after ischemia/reoxygenation in vitro and ...


The River Continuum Redux: Aquatic Insect Diets Reveal The Importance Of Autochthonous Resources In The Salmon River, Idaho, Kathryn Vallis 2011 Loyola University Chicago

The River Continuum Redux: Aquatic Insect Diets Reveal The Importance Of Autochthonous Resources In The Salmon River, Idaho, Kathryn Vallis

Master's Theses

Along a river network, changes in the physical characteristics of a stream - changes articulated by the River Continuum Concept (RCC) - have been predicted to influence stream food webs, particularly the resources supporting aquatic macroinvertebrates. Although diets of macroinvertebrates can track resource availability along the longitudinal gradient of streams, insect diets were not assessed in the original RCC study; rather, insects were grouped into functional feeding groups (FFGs). In addition, global climate change is increasing the frequency and spatial extent of wildfire and beetle outbreaks throughout the western United States and could affect riparian vegetation and the amount of allochthonous inputs ...


Role For Histone Deacetylases In Glucocorticoid Receptor Mediated Transpression Of Natural Killer Cell Activity, Kristin Bush 2011 Loyola University Chicago

Role For Histone Deacetylases In Glucocorticoid Receptor Mediated Transpression Of Natural Killer Cell Activity, Kristin Bush

Master's Theses

During periods of psychosocial distress glucocorticoids (GCs) are known to reduce the lytic activity of natural killer cells (NKCA). Glucocorticoid treatment also reduces acetylation of histone residues; however, the glucocorticoid receptor (GR) lacks deacetylase activity. GR is known to interact with histone deacetylases (HDACs) and with corepressors that mediate gene transrepression. In this investigation, GC induced histone deacetylation was demonstrated to be due to GR recruitment of HDAC1 and the corepressor complex SMRT. These data show that reduced acetylation of immune functional genes associated with NKCA is likely due to histone deacetylation by HDAC1 and transrepression of those genes by ...


Investigation Of A Pharmaceutical Compound With Artificial Streams: Effects Of The Antihistamine Cimetidine On Stream Ecosystem Function., Paul David Hoppe 2011 Loyola University Chicago

Investigation Of A Pharmaceutical Compound With Artificial Streams: Effects Of The Antihistamine Cimetidine On Stream Ecosystem Function., Paul David Hoppe

Master's Theses

Pharmaceutical compounds have been widely detected in surface waters but their effects on stream ecosystems are unknown. Cimetidine (Tagamet®), a widely used H2 histamine antagonist has been detected in surface waters. To measure chronic effects of cimetidine on stream invertebrates, I conducted a long-term (83d) artificial-stream experiment. A range of cimetidine concentrations (0.07 μg L-1 to 70.0 μg L-1) were added to streams supporting populations of the amphipod Gammarus fasciatus and beetle Psephenus herricki. P. herricki individual growth rates were reduced in the presence of cimetidine, but G. fasciatus individual growth rates were not different among treatments. G ...


Human Trophoblast Survival And Invasion In The Developing Placenta: Autocrine Regulation By Hbegf, Philip Jessmon 2011 Wayne State University

Human Trophoblast Survival And Invasion In The Developing Placenta: Autocrine Regulation By Hbegf, Philip Jessmon

Wayne State University Dissertations

HBEGF is a multifunctional protein in early pregnancy that induces cytotrophoblast (CTB) cell differentiation to an invasive phenotype, protects against apoptosis, and is involved in an autocrine signaling mechanism that leads to its own protein synthesis. CTBs exist in a low O2 environment during the first 10 weeks of implantation, during which they invade the decidualized uterine stroma. Inhibitors of intracellular signaling pathways demonstrated that at 20% O2 HBEGF induces an increase in cell migration through the ERK, MAPK14, JNK, or PIK3 pathways downstream of signaling through its ERBB receptors. Also downstream of these four pathways, HBEGF induces ...


Gene Expression Patterns Are Correlated With Genomic And Genic Structure In Soybean, Jenna L. Woody, Andrew J. Severin, Yung-Tsi Bolon, Bindu Joseph, Brian W. Diers, Andrew D. Farmer, Nathan Weeks, Gary J. Muehlbauer, Rex T. Nelson, David Grant, James E. Specht, Michelle A. Graham, Steven B. Cannon, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker 2011 Iowa State University

Gene Expression Patterns Are Correlated With Genomic And Genic Structure In Soybean, Jenna L. Woody, Andrew J. Severin, Yung-Tsi Bolon, Bindu Joseph, Brian W. Diers, Andrew D. Farmer, Nathan Weeks, Gary J. Muehlbauer, Rex T. Nelson, David Grant, James E. Specht, Michelle A. Graham, Steven B. Cannon, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker

Agronomy Publications

Studies have indicated that exon and intron size and intergenic distance are correlated with gene expression levels and expression breadth. Previous reports on these correlations in plants and animals have been conflicting. In this study, next-generation sequence data, which has been shown to be more sensitive than previous expression profiling technologies, were generated and analyzed from 14 tissues. Our results revealed a novel dichotomy. At the low expression level, an increase in expression breadth correlated with an increase in transcript size because of an increase in the number of exons and introns. No significant changes in intron or exon sizes ...


Nanosecond Pulse Electrical Fields Used In Conjunction With Multi-Wall Carbon Nanotubes As A Potential Tumor Treatment, Michael W. Stacey, Christopher Osgood, Bhargava Subhash Kalluri, Wei Cao, Hani Elsayed-Ali, Tarek Abdel-Fattah 2011 Old Dominion University

Nanosecond Pulse Electrical Fields Used In Conjunction With Multi-Wall Carbon Nanotubes As A Potential Tumor Treatment, Michael W. Stacey, Christopher Osgood, Bhargava Subhash Kalluri, Wei Cao, Hani Elsayed-Ali, Tarek Abdel-Fattah

Bioelectrics Publications

The objectives of this communication were to fabricate pure samples of multi-walled carbon nanotubes (MWCNTs) and to determine their toxicity in tumor cell lines. MWCNTs were dispersed in a concentration of the surfactant T80 that was minimally toxic. Cell-type variation in toxicity to MWCNTs was observed but was not significantly different to unexposed controls. Additionally, we investigated the increased cell killing of the pancreatic cancer cell line PANC1 when exposed to ultrashort (nanosecond) pulsed electrical fields (nsPEF) in the presence of MWCNTs as a potential form of cancer therapy. We hypothesized that the unique electronic properties of MWCNTs disrupt cell ...


Seagrass Patch Dynamics In Areas Of Historical Loss In Tampa Bay, Fl, Usa, Kristen A. Kaufman 2011 University of South Florida

Seagrass Patch Dynamics In Areas Of Historical Loss In Tampa Bay, Fl, Usa, Kristen A. Kaufman

Graduate Theses and Dissertations

The study documents seagrass patch dynamics over large spatial extents in Tampa Bay, Florida. Using GIS techniques a set of fine scale seagrass maps was created within locations previously identified as "patchy" seagrass or areas of seagrass loss. Thirty randomly selected landscape windows of various extents were mapped for the years 2004, 2006, and 2008 by visualizing 0.3 m resolution color imagery on-screen at a digitizing scale of 1:500 using a minimum mapping unit of 1 m2. Characteristics of seagrass patches and patterns of seagrass change were quantified using area-based and time interval metrics including total seagrass ...


Role Of Protein Kinase C-Iota In Glioblastoma, Shraddha R. Desai 2011 University of South Florida

Role Of Protein Kinase C-Iota In Glioblastoma, Shraddha R. Desai

Graduate Theses and Dissertations

The focus of this research was to investigate the role of protein kinase C-iota (PKC-é) in the regulation of Bad function, a pro-apoptotic member of the Bcl-2 family and Cdk7 function, a master cell cycle regulator in glioblastoma.

The results were obtained from the human glial tumor derived cell lines, T98G and U87MG. In these cells, PKC-é co-localized and directly associated with Bad as shown by immunofluorescence, immunoprecipitation, and Western blotting. Furthermore, in-vitro kinase activity assay showed that PKC-é directly phosphorylated Bad at phospho specific residues, S112, S136 and S155 which in turn induced inactivation of Bad and disruption of ...


The Role Of Mdm2 In Estrogen-Mediated Breast Cancer Cell Proliferation, Angelika Brekman 2011 Graduate Center, City University of New York

The Role Of Mdm2 In Estrogen-Mediated Breast Cancer Cell Proliferation, Angelika Brekman

All Dissertations, Theses, and Capstone Projects

Estrogen signaling is important in breast cancer development and progression. Mdm2, a negative regulator of the p53 tumor suppressor, is often over-expressed in estrogen receptor positive breast cancers. To study the role of Mdm2 in the estrogen-mediated breast cancer cell proliferation, we examined the effect of estrogen on the p53-Mdm2 pathway in estrogen receptor positive and p53 wild-type MCF-7 breast cancer cells. Estrogen-mediated increase in cell proliferation correlated with increased Mdm2, but no concomitant decrease in the p53 protein level. Blocking Mdm2 expression with inducible shRNA inhibited estrogen-mediated cell proliferation and colony formation in soft agar. Mdm2 knockdown in the ...


Structural And Functional Analysis Of The Type Iii Secretion System From Pseudomonas Fluorescens Q8r1-96, Dmitri V. Mavrodi, Anna Joe, Olga V. Mavrodi, Karl A. Hassan, David M. Weller, Ian T. Paulsen, Joyce E. Loper, James R. Alfano, Linda S. Thomashow 2011 Washington State University

Structural And Functional Analysis Of The Type Iii Secretion System From Pseudomonas Fluorescens Q8r1-96, Dmitri V. Mavrodi, Anna Joe, Olga V. Mavrodi, Karl A. Hassan, David M. Weller, Ian T. Paulsen, Joyce E. Loper, James R. Alfano, Linda S. Thomashow

Faculty Publications from the Center for Plant Science Innovation

Pseudomonas fluorescens Q8r1-96 represents a group of rhizosphere strains responsible for the suppressiveness of agricultural soils to take-all disease of wheat. It produces the antibiotic 2,4-diacetylphloroglucinol and aggressively colonizes the roots of cereal crops. In this study, we analyzed the genome of Q8r1-96 and identified a type III protein secretion system (T3SS) gene cluster that has overall organization similar to that of the T3SS gene cluster of the plant pathogen Pseudomonas syringae. We also screened a collection of 30 closely related P. fluorescens strains and detected the T3SS genes in all but one of them. The Q8r1-96 genome contained ...


Vulture Flight Behavior And Implications For Aircraft Safety, Michael L. Avery, John S. Humphrey, Trey S. Daughtery, Justin W. Fischer, Michael P. Milleson, Eric A. Tillman, William E. Bruce, W. David Walter 2011 USDA/APHIS/WS National Wildlife Research Center

Vulture Flight Behavior And Implications For Aircraft Safety, Michael L. Avery, John S. Humphrey, Trey S. Daughtery, Justin W. Fischer, Michael P. Milleson, Eric A. Tillman, William E. Bruce, W. David Walter

USDA National Wildlife Research Center - Staff Publications

Growing vulture populations represent increasing hazards to civil and military aircraft. To assess vulture flight behavior and activity patterns at the Marine Corps Air Station in Beaufort, South Carolina, we equipped 11 black vultures (Coragyps atratus) and 11 turkey vultures (Cathartes aura) with solarpowered Global Positioning System (GPS) satellite transmitters during a 2-year study (1 Oct 2006–30 Sep 2008). Turkey vultures had larger seasonal home ranges than did black vultures, and 2 turkey vultures made round-trips to Florida. Black vultures consistently spent less time in flight (8.4%) than did turkey vultures (18.9%), and black vultures flew at ...


Acetaminophen And Zinc Phosphide For Lethal Management Of Invasive Lizards Ctenosaura Similis , Michael L. Avery, John D. Eisemann, Kandy L. Keacher, Peter J. Savarie 2011 USDA/APHIS/WS National Wildlife Research Center

Acetaminophen And Zinc Phosphide For Lethal Management Of Invasive Lizards Ctenosaura Similis , Michael L. Avery, John D. Eisemann, Kandy L. Keacher, Peter J. Savarie

USDA National Wildlife Research Center - Staff Publications

Reducing populations of invasive lizards through trapping and shooting is feasible in many cases but effective integrated management relies on a variety of tools, including toxicants. In Florida, using wild-caught non-native black spiny-tailed iguanas Ctenosaura similis, we screened acetaminophen and zinc phosphide to determine their suitability for effective population management of this prolific invasive species. Of the animals that received acetaminophen, none died except at the highest test dose, 240 mg per lizard, which is not practical for field use. Zinc phosphide produced 100% mortality at dose levels as little as 25 mg per lizard, equivalent to about 0.5 ...


Modeling Connectivity Of Black Bears In A Desert Sky Island Archipelago, Todd C. Atwood, Julie K. Young, Jon P. Beckmann, Stewart W. Breck, Jennifer A. Fike, Olin E. Rhodes Jr., Kirby D. Bristow 2011 USDA National Wildlife Research Center, Fort Collins, CO

Modeling Connectivity Of Black Bears In A Desert Sky Island Archipelago, Todd C. Atwood, Julie K. Young, Jon P. Beckmann, Stewart W. Breck, Jennifer A. Fike, Olin E. Rhodes Jr., Kirby D. Bristow

USDA National Wildlife Research Center - Staff Publications

Landscape features such as rivers, mountains, desert basins, roads, and impermeable man-made structures may influence dispersal and gene flow among populations, thereby creating spatial structure across the landscape. In the US–Mexico borderland, urbanization and construction of the border fence have the potential to increase genetic subdivision and vulnerability to isolation in large mammal populations by bisecting movement corridors that have enabled dispersal between adjacent Sky Island mountain ranges. We examined genetic variation in black bears (Ursus americanus) from three regions in central and southern Arizona, US, to assess genetic and landscape connectivity in the US–Mexico border Sky Islands ...


Rock Pigeon Use Of Livestock Facilities In Northern Colorado: Implications For Improving Farm Bio-Security., James C. Carlson, Larry Clark, Michael F. Antolin, M. D. Salman 2011 USDA/APHIS/WS National Wildlife Research Center

Rock Pigeon Use Of Livestock Facilities In Northern Colorado: Implications For Improving Farm Bio-Security., James C. Carlson, Larry Clark, Michael F. Antolin, M. D. Salman

USDA National Wildlife Research Center - Staff Publications

Rock pigeons (Columba livia) have been implicated in the spread of pathogens within commercial livestock facilities. Currently, there is no data characterizing pigeon habitat use and movement patterns within and among commercial livestock facilities. To better understand the capacity for pigeons to spread pathogens, we used radio-telemetry techniques to estimate the home-range, travel distance, activity, and habitat use of pigeons roosting on and off dairies and feedlots in western Weld County, Colorado. Our observations suggest that pigeons roosting on (resident) and off (nonresident) livestock facilities use habitat differently. Nonresident pigeons used larger home-range areas than did resident pigeons. Nonresident pigeons ...


Two Distinct Roles Of Arabidopsis Homolog Of Trithorax1 (Atx1) At Promoters And Within Transcribed Regions Of Atx1-Regulated Genes, Yong Ding, Zoya Avramova, Michael E. Fromm 2011 University of Nebraska - Lincoln

Two Distinct Roles Of Arabidopsis Homolog Of Trithorax1 (Atx1) At Promoters And Within Transcribed Regions Of Atx1-Regulated Genes, Yong Ding, Zoya Avramova, Michael E. Fromm

Faculty Publications from the Center for Plant Science Innovation

The Arabidopsis thaliana trithorax-like protein, ATX1, shares common structural domains, has similar histone methyltransferase (HMT) activity, and belongs in the same phylogenetic subgroup as its animal counterparts. Most of our knowledge of the role of HMTs in trimethylating lysine 4 of histone H3 (H3K4me3) in transcriptional regulation comes from studies of yeast and mammalian homologs. Little is known about the mechanism by which ATX1, or any other HMT of plant origin, affects transcription. Here, we provide insights into how ATX1 influences transcription at regulated genes, playing two distinct roles. At promoters, ATX1 is required for TATA binding protein (TBP) and ...


Myth4 And Ferm Have Overlapping And Distinct Roles In The Function Of Myo1, A Class Xiv Myosin In Tetrahymena Thermophila, Michael Gotesman 2011 Graduate Center, City University of New York

Myth4 And Ferm Have Overlapping And Distinct Roles In The Function Of Myo1, A Class Xiv Myosin In Tetrahymena Thermophila, Michael Gotesman

All Dissertations, Theses, and Capstone Projects

MyTH4 and FERM are conserved tail domains in myosin classes VII, X, XII, XIV, XV and in MyoG. Myo1, a class XIV myosin in Tetrahymena thermophila, contains MyTH4 and FERM. Previous studies have shown that Myo1 localizes to phagosomes, the cytoskeleton, and the macronucleus, and that phagosome trafficking and division of the macronucleus are affected in a MYO1 knockout. To investigate the roles for MyTH4 and FERM in the function of Myo1, GFP-tagged MyTH4, FERM, and truncated FERM were separately overexpressed in Tetrahymena. Actin antibody coprecipitated tubulin, GFP-MyTH4, and GFP-FERM. GFP-MyTH4 and GFP-FERM cosedimented with either exogenous microtubules or exogenous ...


Influenza A And Flavivirus Manipulation Of Cell Death, Jeffrey E. McLean 2011 The Graduate Center, City University of New York

Influenza A And Flavivirus Manipulation Of Cell Death, Jeffrey E. Mclean

All Dissertations, Theses, and Capstone Projects

Viruses employ a variety of strategies to manipulate cell fate and maximize their replication potential during infection. Interacting specifically with cell death pathways allows viruses direct control over the cellular decision to survive or die during infection.

Influenza A virus, of the family Orthomyxoviridae, encodes proteins that interact with the cell at several points along the apoptosis pathway to induce apoptosis. We demonstrate that caspase activation through pro-apoptotic Bax, a downstream target of Bcl-2, is a critical determinant of the nature of cell death induced by Influenza A infection. Influenza A virus cannot establish an apoptotic response without functional Bax ...


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