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Initial Events Associated With Virus Pbcv-1 Infection Of Chlorella Nc64a, Gerhard Thiel, Anna Moroni, David Dunigan, James L. Van Etten 2010 Universitat Darmstadt

Initial Events Associated With Virus Pbcv-1 Infection Of Chlorella Nc64a, Gerhard Thiel, Anna Moroni, David Dunigan, James L. Van Etten

Papers in Plant Pathology

Chlorella viruses (or chloroviruses) are very large, plaque-forming viruses. The viruses are multilayered structures containing a large double-stranded DNA genome, a lipid bilayered membrane, and an outer icosahedral capsid shell. The viruses replicate in certain isolates of the coccal green alga, Chlorella. Sequence analysis of the 330-kbp genome of Paramecium bursaria Chlorella virus 1 (PBCV-1), the prototype of the virus family Phycodnaviridae, reveals <365 protein-encoding genes and 11 tRNA genes. Products of about 40% of these genes resemble proteins of known function, including many that are unexpected for a virus. Among these is a virus-encoded protein, called Kcv, which forms a functional K+ channel. This chapter focuses on the initial steps in virus infection and provides a plausible role for the function of the viral K+ channel in lowering the turgor pressure of the host. This step appears to be a prerequisite for delivery of the viral genome into the host.


Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers 2010 University of Illinois

Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Agronomy & Horticulture -- Faculty Publications

The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from ‘Dowling’. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping was carried ...


Meloidogyne Spp. Infecting Ornamental Plants In Florida, J. A. Brito, R. Kaur, R. Cetintas, J. D. Stanley, M. L. Mendes, Thomas O. Powers, D. W. Dickson 2010 Division of Plant Industry

Meloidogyne Spp. Infecting Ornamental Plants In Florida, J. A. Brito, R. Kaur, R. Cetintas, J. D. Stanley, M. L. Mendes, Thomas O. Powers, D. W. Dickson

Papers in Plant Pathology

A total of 206 root samples were collected from ornamental plants growing in ornamental nurseries and various landscapes in Florida. Isozyme phenotypes, especially esterase (EST) and malate dehydrogenase (MDH) were the main methods used to identify the root-knot nematode species. When needed, the morphology of female perineal patterns, morphometric characters and mitochondrial DNA were used to aid in the identification. Six Meloidogyne spp., M. arenaria , M. floridensis , M. graminis , M. incognita , M. javanica and M. mayaguensis were found infecting ornamental plants in Florida. As previously reported EST activity was of highest diagnostic value to identify Meloidogyne spp. found in this ...


Heterologous Minor Coat Proteins Of Citrus Tristeza Virus Strains Affect Encapsidation, But The Coexpression Of Hsp70h And P61 Restores Encapsidation To Wild-Type Levels, Satyanarayana Tatineni, Siddarame Gowda, William O. Dawson 2010 USDA-ARS

Heterologous Minor Coat Proteins Of Citrus Tristeza Virus Strains Affect Encapsidation, But The Coexpression Of Hsp70h And P61 Restores Encapsidation To Wild-Type Levels, Satyanarayana Tatineni, Siddarame Gowda, William O. Dawson

Papers in Plant Pathology

The long flexuous bipolar virions of Citrus tristeza virus (CTV), a Closterovirus, are encapsidated with two capsid proteins at opposite ends: the minor coat protein (CPm) encapsidates the 5′ 630 nts of the genomic RNA and the major coat protein encapsidates the remainder of the genome. In this study, we found encapsidation of CTV CPm in the absence of other assembly-related proteins is highly specific in contrast to most plant viruses that allow virion assembly by a range of heterologous coat proteins. Heterologous CPms with 95–96% amino acid identity from related strains in CTV-CPm, a replicon with CPm as ...


Selecting Strawberry Cultivars For Winter Greenhouse Production, Ellen T. Paparozzi, Stacy A. Adams, George E. Meyer, M. Elizabeth Conley, Vicki L. Schlegel, Erin E. Blankenship, Paul E. Read 2010 University of Nebraska-Lincoln

Selecting Strawberry Cultivars For Winter Greenhouse Production, Ellen T. Paparozzi, Stacy A. Adams, George E. Meyer, M. Elizabeth Conley, Vicki L. Schlegel, Erin E. Blankenship, Paul E. Read

Agronomy & Horticulture -- Faculty Publications

Costs of transportation and food safety concerns have spawned an increase in public support of locally grown vegetables and fruit. With this in mind, a two-phase investigation was planned with the goal of combining low start up costs for sustainable greenhouse production with selection of strawberry cultivars that would provide the greatest number and largest size of berries. Additionally, berries from each cultivar will be analyzed for their beneficial nutraceutical properties to determine if there is a difference among cultivars.


An Integrative Approach To Genomic Introgression Mapping, Andrew J. Severin, Gregory A. Peiffer, Wayne W. Xu, D. L. Hyten, Bruna Bucciarelli, Jamie A. O'Rourke, Yung-Tsi Bolon, David Grant, Andrew D. Farmer, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker, Robert M. Stupar 2010 Iowa State University

An Integrative Approach To Genomic Introgression Mapping, Andrew J. Severin, Gregory A. Peiffer, Wayne W. Xu, D. L. Hyten, Bruna Bucciarelli, Jamie A. O'Rourke, Yung-Tsi Bolon, David Grant, Andrew D. Farmer, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker, Robert M. Stupar

Agronomy & Horticulture -- Faculty Publications

Near-isogenic lines (NILs) are valuable genetic resources for many crop species, including soybean (Glycine max). The development of new molecular platforms promises to accelerate the mapping of genetic introgressions in these materials. Here, we compare some existing and emerging methodologies for genetic introgression mapping: single-feature polymorphism analysis, Illumina GoldenGate single nucleotide polymorphism (SNP) genotyping, and de novo SNP discovery via RNA-Seq analysis of next-generation sequence data. We used these methods to map the introgressed regions in an iron-inefficient soybean NIL and found that the three mapping approaches are complementary when utilized in combination. The comparative RNA-Seq approach offers several additional ...


Soil And Root Populations Of Fluorescent Pseudomonas Spp. Associated With Seedlings And Field-Grown Plants Are Affected By Sorghum Genotype, Deanna L. Funnell-Harris, Jeffrey F. Pedersen, Scott E. Sattler 2010 USDA-ARS

Soil And Root Populations Of Fluorescent Pseudomonas Spp. Associated With Seedlings And Field-Grown Plants Are Affected By Sorghum Genotype, Deanna L. Funnell-Harris, Jeffrey F. Pedersen, Scott E. Sattler

Papers in Plant Pathology

Sorghum [Sorghum bicolor (L.) Moench] is valued for bioenergy, feed and food. Potential of sorghum genotypes to support differing populations of root- and soil-associated fluorescent Pseudomonas spp. or Fusarium spp., in two soils, was assessed. Culturable pseudomonads were enumerated from roots and soil of sorghum (Redlan and RTx433) and wheat (Lewjain) seedlings repeatedly grown in cycled soils in the growth chamber. Pseudomonads and Fusarium spp. were assessed from roots and soil of field-grown sorghum along with biological control traits hydrogen cyanide (HCN) and 2,4-diacetylphlorogluconol (phl) production. After four 4-week cycles, soil associated with Redlan seedlings had greater numbers of ...


Brown Midrib Mutations And Their Importance To The Utilization Of Maize, Sorghum, And Pearl Millet Lignocellulosic Tissues, Scott E. Sattler, Deanna L. Funnell-Harris, Jeffrey F. Pedersen 2010 USDA-ARS

Brown Midrib Mutations And Their Importance To The Utilization Of Maize, Sorghum, And Pearl Millet Lignocellulosic Tissues, Scott E. Sattler, Deanna L. Funnell-Harris, Jeffrey F. Pedersen

Papers in Plant Pathology

Brown midrib mutants have been isolated in maize (Zea mays), sorghum (Sorghum bicolor) and pearl millet (Pennisetum glaucum) arising by either spontaneous or chemical mutagenesis. The characteristic brown coloration of the leaf mid veins is associated with reduced lignin content and altered lignin composition, traits useful to improve forage digestibility for livestock. Brown midrib phenotype is correlated with two homologous loci in maize (bm1 and bm3) and sorghum (bmr6 and bmr12), which encode cinnamyl alcohol dehydrogenase (CAD) and a caffeic O-methyl transferase (COMT). These enzymes are involved in the last two steps of monolignol biosynthesis. In maize, bm phenotype is ...


Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan 2010 USDA-ARS, Soybean Genomics and Improvement Lab., Beltsville, MD

Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan

Agronomy & Horticulture -- Faculty Publications

Simple sequence repeat (SSR) genetic markers, also referred to as microsatellites, function in map-based cloning and for marker-assisted selection in plant breeding. The objectives of this study were to determine the abundance of SSRs in the soybean genome and to develop and test soybean SSR markers to create a database of locus-specific markers with a high likelihood of polymorphism. A total of 210,990 SSRs with di-, tri-, and tetranucleotide repeats of five or more were identified in the soybean whole genome sequence (WGS) which included 61,458 SSRs consisting of repeat units of di- (≥10), tri- (≥8), and tetranucleotide ...


Efficacy Of Singular And Stacked Brown Midrib 6 And 12 In The Modification Of Lignocellulose And Grain Chemistry, Scott E. Sattler, Deanna L. Funnell-Harris, Jeffrey F. Pedersen 2010 USDA-ARS

Efficacy Of Singular And Stacked Brown Midrib 6 And 12 In The Modification Of Lignocellulose And Grain Chemistry, Scott E. Sattler, Deanna L. Funnell-Harris, Jeffrey F. Pedersen

Agronomy & Horticulture -- Faculty Publications

In sorghum, brown midrib (bmr) 6 and 12 impair the last two steps of monolignol synthesis. bmr genes were introduced into grain sorghum to improve the digestibility of lignocellulosic tissues for grazing or bioenergy uses following grain harvest. Near-isogenic grain sorghum hybrids (AWheatland X RTx430) were developed containing bmr6, bmr12, and the bmr6 bmr12 double mutant (stacked), and their impacts were assessed in a two-year field study. The bmr genes did not significantly impact grain or lignocellulosic tissue yield. Lignocellulosic tissue from bmr6, bmr12, and stacked hybrids had reduced lignin content and increased in vitro dry matter digestibility (IVDMD) compared ...


Crop Registration: The Pathway To Public Access Of Plant Genetic Materials To Build Crops For The Future, D. D. Ellis, K. A. Garland-Campbell, J. A. Grotenhuis, M. M. Jenderek, Jeffrey F. Pedersen 2010 USDA, ARS, National Center for Genetic Resources Preservation

Crop Registration: The Pathway To Public Access Of Plant Genetic Materials To Build Crops For The Future, D. D. Ellis, K. A. Garland-Campbell, J. A. Grotenhuis, M. M. Jenderek, Jeffrey F. Pedersen

Agronomy & Horticulture -- Faculty Publications

Starting in 1926 and continuing for 80+ yr, 11,241 crop genetic materials have been registered as of 31 Dec. 2008. The crop registration process is an important pathway to publically describe and document new and useful genetic materials and to incorporate these into the public domain via the National Plant Germplasm System (NPGS). Crop Registration materials are now searchable via the Germplasm Resources Information Network (GRIN) and demand for registered materials remains strong with more than 9150 registered accessions distributed in the past 26 yr by the NPGS. Guidelines continue to evolve to accommodate global factors effecting germplasm exchange ...


Heterosis In Sweet Sorghum And Selection Of A New Sweet Sorghum Hybrid For Use In Syrup Production In Appalachia, T. W. Pfeiffer, M. J. Bitzer, J. J. Toy, Jeffrey F. Pedersen 2010 University of Kentucky

Heterosis In Sweet Sorghum And Selection Of A New Sweet Sorghum Hybrid For Use In Syrup Production In Appalachia, T. W. Pfeiffer, M. J. Bitzer, J. J. Toy, Jeffrey F. Pedersen

Agronomy & Horticulture -- Faculty Publications

Although heterosis is well established in grain and forage sorghum [Sorghum bicolor (L.) Moench], reports of heterosis in sweet sorghum are limited to results from grain sorghum × sweet sorghum hybrids. Recent development of cytoplasmic male-sterile sweet sorghum lines allows creation of sweet sorghum hybrids for research and industry. Male sterility may also affect allocation of photosynthate to plant parts, creating the potential to increase sugar content in stems by eliminating seed as a sink. The objectives of this study were to compare performance of A3 cytoplasmic male-sterile lines and A3 cytoplasmic male-sterile hybrids to fertile B3 counterparts ...


Isolation Of The Phycodnavirus Pbcv-1 By Biological Laser Printing, Lisa A. Fitzgerald, Peter K. Wu, James R. Gurnon, Justin C. Biffinger, Bradley R. Ringeisen, James L. Van Etten 2010 U.S. Naval Research Laboratory

Isolation Of The Phycodnavirus Pbcv-1 By Biological Laser Printing, Lisa A. Fitzgerald, Peter K. Wu, James R. Gurnon, Justin C. Biffinger, Bradley R. Ringeisen, James L. Van Etten

Papers in Plant Pathology

The Phycodnaviridae family of viruses is diverse genetically but similar morphologically. These viruses infect eukaryotic algal hosts from both fresh and marine waters, and are an important component of aqueous environments. They play important roles in the dynamics of algal blooms, nutrient cycling, algal community structure, and possibly gene transfer between organisms. As such, it is important to identify new viruses within the Phycodnaviridae family. Biological laser printing (BioLP) was used to isolate single virus particles from solution. BioLP prints droplets containing a single virus particle directly onto a host medium, thereby enabling viruses to be isolated from unmodified samples ...


Population- And Genome-Specific Patterns Of Linkage Disequilibrium And Snp Variation In Spring And Winter Wheat (Triticum Aestivum L.), Shiaoman Chao, Jorge Dubcovsky, Jan Dvorak, Ming-Cheng Luo, P. Stephen Baenziger, Rustam Matnyazov, Dale R. Clark, Luther E. Talbert, James A. Anderson, Susanne Dreisigacker, Karl Glover, Jianli Chen, Kim Campbell, Phil L. Bruckner, Jackie C. Rudd, Scott Haley, Brett F. Carver, Sid Perry, Mark E. Sorrells, Eduard D. Akhunov 2010 USDA-ARS

Population- And Genome-Specific Patterns Of Linkage Disequilibrium And Snp Variation In Spring And Winter Wheat (Triticum Aestivum L.), Shiaoman Chao, Jorge Dubcovsky, Jan Dvorak, Ming-Cheng Luo, P. Stephen Baenziger, Rustam Matnyazov, Dale R. Clark, Luther E. Talbert, James A. Anderson, Susanne Dreisigacker, Karl Glover, Jianli Chen, Kim Campbell, Phil L. Bruckner, Jackie C. Rudd, Scott Haley, Brett F. Carver, Sid Perry, Mark E. Sorrells, Eduard D. Akhunov

Papers in Plant Pathology

Background: Single nucleotide polymorphisms (SNPs) are ideally suited for the construction of high-resolution genetic maps, studying population evolutionary history and performing genome-wide association mapping experiments. Here, we used a genome-wide set of 1536 SNPs to study linkage disequilibrium (LD) and population structure in a panel of 478 spring and winter wheat cultivars (Triticum aestivum) from 17 populations across the United States and Mexico.

Results: Most of the wheat oligo pool assay (OPA) SNPs that were polymorphic within the complete set of 478 cultivars were also polymorphic in all subpopulations. Higher levels of genetic differentiation were observed among wheat lines within ...


Morphological And Molecular Characterization Of Discocriconemella Inarata, An Endemic Nematode From North American Native Tallgrass Prairies, Thomas O. Powers, Timothy Harris, Rebecca Higgins, Lisa Sutton, Kirsten S. Powers 2010 University of Nebraska - Lincoln

Morphological And Molecular Characterization Of Discocriconemella Inarata, An Endemic Nematode From North American Native Tallgrass Prairies, Thomas O. Powers, Timothy Harris, Rebecca Higgins, Lisa Sutton, Kirsten S. Powers

Papers in Plant Pathology

Discocriconemella inarata, a plant parasitic nematode species originally discovered in a virgin tallgrass prairie in northwest Iowa, was re-examined by molecular and morphological analyses of topotype material. This species has never been recorded in cultivated fields and could potentially serve as an indicator for high quality prairie habitats. DNA sequence from a conserved 3’ portion of the 18S ribosomal gene exhibited an identical match between D. inarata topotype specimens and topotype specimens of Mesocriconema xenoplax from Fresno, California. Higher resolution sequence analyses using the internal transcribed spacer 1 (ITS1) and a portion of the mitochondrial gene cytochrome b (cytb) allowed ...


Spring Seed Guide 2010: Nebraska Variety And Hybrid Tests -- Soybeans, Teshome Regassa, Robert N. Klein, Bruce Anderson, Charles Shapiro, Jim Krall 2010 University of Nebraska-Lincoln

Spring Seed Guide 2010: Nebraska Variety And Hybrid Tests -- Soybeans, Teshome Regassa, Robert N. Klein, Bruce Anderson, Charles Shapiro, Jim Krall

Agronomy & Horticulture -- Faculty Publications

NEBRASKA SOYBEAN VARIETY TESTS 2009

Crop Production Summary: The November 2009 estimated soybean yield for Nebraska was 52 bushels per acre from 4.800 million harvested acres. The total production of soybeans for the state was forecasted at 247 million bushels. These estimates are from the November National Agricultural Statistics Service.


Upper Canopy Collection And Identification Of Grapevines (Vitis) From Selected Forests In The Southeastern United States, Sydney E. Everhart 2010 University of Nebraska-Lincoln

Upper Canopy Collection And Identification Of Grapevines (Vitis) From Selected Forests In The Southeastern United States, Sydney E. Everhart

Papers in Plant Pathology

Woody grapevines (Vitis spp.) are common in the deciduous forests of the southeastern United States. Their growth habit makes leaf collection challenging and polymorphic leaves make identification of species difficult. Mature grapevines can grow up to 48 cm in diameter at breast height and reach the upper canopy of trees more than 35 m in height. Leaf morphology is the most readily available character used for species identification. However, most mature grapevines do not produce leaves below the upper canopy and if they do, these leaves are morphologically indistinguishable from other species. In order to sample leaves from mature grapevines ...


Importance Of Myxomycetes In Biological Research And Teaching, Harold W. Keller, Sydney E. Everhart 2010 University of Central Missouri, Warrensburg

Importance Of Myxomycetes In Biological Research And Teaching, Harold W. Keller, Sydney E. Everhart

Papers in Plant Pathology

Myxomycetes, the true slime molds, are highlighted in research and teaching that emphasizes various stages of the life cycle as experimental models. Past and current phylogenetic classifications of Myxomycetes on the tree of life are presented. Life cycle stages are illustrated, described, and discussed. Simple laboratory demonstrations and experiments are described that include spore germination, spore release, and moist chamber cultures utilizing organic matter from various microhabitats. Novel compounds isolated from fruiting bodies and plasmodia of 22 myxomycete species are tabulated, some of which exhibit biological activity that function as antibiotics, antimicrobials, and are cytotoxic to cancer cells. Aeroallergens include ...


Regulation Of Septum Formation By The Bud3–Rho4 Gtpase Module In Aspergillus Nidulans, Haoyu Si, Daniela Justa-Schuch, Stephan Seiler, Steven D. Harris 2010 University of Nebraska - Lincoln

Regulation Of Septum Formation By The Bud3–Rho4 Gtpase Module In Aspergillus Nidulans, Haoyu Si, Daniela Justa-Schuch, Stephan Seiler, Steven D. Harris

Papers in Plant Pathology

The ability of fungi to generate polarized cells with a variety of shapes likely reflects precise temporal and spatial control over the formation of polarity axes. The bud site selection system of Saccharomyces cerevisiae represents the best-understood example of such a morphogenetic regulatory system. However, the extent to which this system is conserved in the highly polarized filamentous fungi remains unknown. Here, we describe the functional characterization and localization of the Aspergillus nidulans homolog of the axial bud site marker Bud3. Our results show that AnBud3 is not required for polarized hyphal growth per se, but is involved in septum ...


Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean, Ki-Seung Kim, Stephanie Bellendir, Karen A. Hudson, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers 2010 University of Illinois

Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean, Ki-Seung Kim, Stephanie Bellendir, Karen A. Hudson, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Agronomy & Horticulture -- Faculty Publications

The soybean aphid (Aphis glycines Matsumura) is an important soybean [Glycine max (L.) Merr.] pest in North America. The dominant aphid resistance gene Rag1 was previously mapped from the cultivar ‘Dowling’ to a 12 cM marker interval on soybean chromosome 7 (formerly linkage group M). The development of additional genetic markers mapping closer to Rag1 was needed to accurately position the gene to improve the effectiveness of markerassisted selection (MAS) and to eventually clone it. The objectives of this study were to identify single nucleotide polymorphisms (SNPs) near Rag1 and to position these SNPs relative to Rag1. To generate a ...


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