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Association Of Brain-Derived Neurotrophic Factor (Val66met) Genetic Polymorphism With Methamphetamine Dependence In A Malaysian Population, Zahurin Mohamed 2009 University of Malaya

Association Of Brain-Derived Neurotrophic Factor (Val66met) Genetic Polymorphism With Methamphetamine Dependence In A Malaysian Population, Zahurin Mohamed

Zahurin Mohamed

Methamphetamine is a highly addictive psychostimulant that has surged in popularity worldwide in the last decade. Brain-derived neurotrophic factor (BDNF), a member of the neurotrophic factor family, is widely expressed in the adult mammalian brain and plays an important role in the long-term survival, differentiation, and outgrowth of neurons. Previous studies suggested that the BDNF gene may be involved in the mechanisms underlying substance dependence. This study investigated the association of the BDNF gene Val66Met polymorphism with methamphetamine dependence and with psychosis in a Malaysian population with different ethnicities. The BDNF Val66Met polymorphism was genotyped by PCR-RFLP in 186 male ...


Nutrient Analysis Of Varying Socioeconomic Status Home Food Environments In New Jersey State, Scott D. Schefske, Cara L. Cuite, Anne C. Bellows, Carol Byrd-Bredbenner, Teresa Vivar, Holly Rapport, William K. Hallman 2009 Syracuse University

Nutrient Analysis Of Varying Socioeconomic Status Home Food Environments In New Jersey State, Scott D. Schefske, Cara L. Cuite, Anne C. Bellows, Carol Byrd-Bredbenner, Teresa Vivar, Holly Rapport, William K. Hallman

Anne Bellows

Home food inventories of Oaxacan Mexican-American and African-American families of low-socioeconomic status living in an urban area in New Jersey with at least one child under the age of 12 were conducted using Universal Product Code scanning. The African-American and Oaxacan household food supplies were compared with a sample of White households, also with at least one child under the age of 12, not of low-socioeconomic status. Nutrient Adequacy Ratios for total fat, saturated fat, cholesterol, sodium, total carbohydrate, sugar, dietary fiber, protein, vitamin A, vitamin C, calcium, and iron were used to quantify the adequacy of household food supplies ...


Regional Assemblages Of Lygus (Heteroptera: Miridae) In Montana Canola Fields, Andrew W. Lenssen, R. A. Ritter, Sue L. Blodgett, M. L. Taper 2009 United States Department of Agriculture

Regional Assemblages Of Lygus (Heteroptera: Miridae) In Montana Canola Fields, Andrew W. Lenssen, R. A. Ritter, Sue L. Blodgett, M. L. Taper

Andrew W. Lenssen

Sweep net sampling of canola (Brassica napus L.) was conducted in 2002 and 2003 to determine Lygus (Heteroptera: Miridae) species composition and parasitism levels in four regions of Montana. Regardless of region or seasonal change, Lygus elisus (Van Duzee) was the dominant species in all canola fields sampled, averaging 60–99% of the total adult populations. Lygus borealis (Kelton), Lygus keltoni (Schwartz) and Lygus lineolaris (Palisot) were detected at much lower levels. Total lygus population density was greatest in the southwest and central regions. The northeast and southwest regions had the greatest lygus species diversity. The proportion of L. elisus ...


Direct Polymerase Chain Reaction-Based Detection Of Cercospora Beticola In Field Soils, Andrew W. Lenssen, R. T. Lartey, T. C. Caesar-TonThat, J. Eckhoff, S. L. Hanson, R. G. Evans 2009 United States Department of Agriculture

Direct Polymerase Chain Reaction-Based Detection Of Cercospora Beticola In Field Soils, Andrew W. Lenssen, R. T. Lartey, T. C. Caesar-Tonthat, J. Eckhoff, S. L. Hanson, R. G. Evans

Andrew W. Lenssen

Cercospora beticola, the causal agent of Cercospora leaf spot of sugar beet, survives as pseudostromata in infected sugar beet residues in the soil. Under optimal conditions, overwintering propagules germinate and produce conidia that are dispersed as primary inoculum to initiate infection in sugar beet. We developed a polymerase chain reaction (PCR) technique for rapid detection of C. beticola in field soils. Total DNA was first isolated from soil amended with C. beticola culture using the PowerSoil DNA Kit. The purified DNA was subjected to PCR in Extract- N-Amp PCR mix with CBACTIN primers over 35 cycles. The amplified products were ...


Effects Of Tillage On Microbial Populations Associated To Soil Aggregation In Dryland Spring Wheat System, Andrew W. Lenssen, TheCan Caesar-TonThat, Anthony J. Caesar, Upendra M. Sainju, John F. Gaskin 2009 United States Department of Agriculture

Effects Of Tillage On Microbial Populations Associated To Soil Aggregation In Dryland Spring Wheat System, Andrew W. Lenssen, Thecan Caesar-Tonthat, Anthony J. Caesar, Upendra M. Sainju, John F. Gaskin

Andrew W. Lenssen

Tillage may influence the microbial populations involved in soil aggregation.We evaluated the effects of no till (NT) and conventional tillage (CT, tillage depth about 7 cm) continuous spring wheat system on culturable heterotrophic bacterial communities predominant in microaggregates (0.25e0.05 mm) and on soil-aggregating basidiomycete fungi in aggregate-size classes (4.75e2.00, 2.00e0.25, and 0.25e0.05 mm) at 0e20 cm depth of a Williams loam (fine-loamy, mixed, Typic Argiustolls) in dryland Montana, USA. Enzyme-linked immunosorbent assay used to quantify antigenic response to basidiomycete cellwalls,was higher in NT than in CT in 4.75e2.00 ...


The Apoptotic Effect Of 1 ' S-1 '-Acetoxychavicol Acetate From Alpinia Conchigera On Human Cancer Cells, Noor Hasima Nagoor 2009 University of Malaya

The Apoptotic Effect Of 1 ' S-1 '-Acetoxychavicol Acetate From Alpinia Conchigera On Human Cancer Cells, Noor Hasima Nagoor

Noor Hasima Nagoor

1'-(S)-1'-Acetoxychavicol acetate (ACA) isolated from the Malaysian ethnomedicinal plant Alpinia conchigera Griff. was investigated for its potential as an anticancer drug. In this communication, we describe the cytotoxic and apoptotic properties of ACA on five human tumour cell lines. Data from MTT cell viability assays indicated that ACA induced both time-and dose-dependent cytotoxicity on all tumour cell lines tested and had no adverse cytotoxic effects on normal cells. Total mortality of the entire tumour cell population was achieved within 30 hrs when treated with ACA at 40.0 mu M concentration. Flow cytometric analysis for annexin-V and ...


Changes In Light Levels With Loss Of Eastern Hemlock (Tsuga Canadensis) At A Southern Appalachian Headwater Stream: Implications For Brook Trout (Salvelinus Fontinalis), L. A. Siderhurst, Heather P. Griscom, M. Hudy, Z. J. Bortolot 2009 James Madison University

Changes In Light Levels With Loss Of Eastern Hemlock (Tsuga Canadensis) At A Southern Appalachian Headwater Stream: Implications For Brook Trout (Salvelinus Fontinalis), L. A. Siderhurst, Heather P. Griscom, M. Hudy, Z. J. Bortolot

Heather P. Griscom

No abstract provided.


Tuberculosis: An Eight Year (2000-2007) Retrospective Study At The University Of Malaya Medical Centre (Ummc), Kuala Lumpur, Malaysia, Jamaiah Ibrahim 2009 University of Malaya

Tuberculosis: An Eight Year (2000-2007) Retrospective Study At The University Of Malaya Medical Centre (Ummc), Kuala Lumpur, Malaysia, Jamaiah Ibrahim

Jamaiah Ibrahim

This was an eight year (2000-2007) retrospective study of tuberculosis in patients admitted to the UMMC. A total of 131 cases were analyzed. Malays constituted the most cases, (43%), followed by Chinese (22%), Indians (17%) and others (18%). The majority of cases were within the 21-60 year old age group, which constituted 69.5% of the total. Males were more commonly affected (65%). Most cases were reported among Malaysians (83%). The majority of patients were unemployed (39%), followed by housewives (10%), laborers (9%), students (8%), shop assistants (7%), and other occupations (27%) The most common presenting complaints were prolonged productive ...


Indigenous Ectosymbiotic Bacteria Associated With Diverse Hydrothermal Vent Invertebrates, Shana K. Goffredi 2009 Occidental College

Indigenous Ectosymbiotic Bacteria Associated With Diverse Hydrothermal Vent Invertebrates, Shana K. Goffredi

Shana Goffredi

Symbioses involving bacteria and invertebrates contribute to the biological diversity and high productivity of both aquatic and terrestrial environments. Well-known examples from chemosynthetic deep-sea hydrothermal vent environments involve ectosymbiotic microbes associated with the external surfaces of marine invertebrates. Some of these ectosymbioses confer protection or defence from predators or the environment itself, some are nutritional in nature, and many still are of unknown function. Several recently discovered hydrothermal vent invertebrates, including two populations of yeti crab (Kiwa spp.), a limpet (Symmetromphalus aff. hageni), and the scaly-foot snail (as yet undescribed), support a consortium of diverse bacteria. Comparisons of these ectosymbioses ...


Poleward Transport Of Tpx2 In The Mammalian Mitotic Spindle Requires Dynein, Eg5, And Microtubule Flux, Patricia Wadsworth, N. Ma, S. Tulu, N. Ferenz, C. Fagerstrom, A. Mogilner 2009 University of Massachusetts - Amherst

Poleward Transport Of Tpx2 In The Mammalian Mitotic Spindle Requires Dynein, Eg5, And Microtubule Flux, Patricia Wadsworth, N. Ma, S. Tulu, N. Ferenz, C. Fagerstrom, A. Mogilner

Patricia Wadsworth

TPX2 is a Ran-regulated spindle assembly factor that is required for kinetochore fiber formation and activation of the mitotic kinase Aurora A. TPX2 is enriched near spindle poles and is required near kinetochores, suggesting that it undergoes dynamic relocalization throughout mitosis. Using photoactivation, we measured the movement of PA-GFP-TPX2 in the mitotic spindle. TPX2 moves poleward in the half-spindle and is static in the interzone and near spindle poles. Poleward transport of TPX2 is sensitive to inhibition of dynein or Eg5 and to suppression of microtubule flux with nocodazole or antibodies to Kif2a. Poleward transport requires the C terminus of ...


Toward Genuine Rodent Welfare: Response To Reviewer Comments, Jonathan P. Balcombe 2009 Humane Society Institute for Science and Policy

Toward Genuine Rodent Welfare: Response To Reviewer Comments, Jonathan P. Balcombe

Jonathan Balcombe, Ph.D.

No abstract provided.


A New Genus To Accommodate Gymnopus Acervatus (Agaricales), Karen Hughes, David A. Mather, Ronald H. Peterson 2009 University of Tennessee, Knoxville

A New Genus To Accommodate Gymnopus Acervatus (Agaricales), Karen Hughes, David A. Mather, Ronald H. Peterson

Karen Hughes

Phylogenies based on ITS and LSU nrDNA sequences show Agaricus (Gymnopus) acervatus as unique within the Gymnopus/Rhodocollybia complex. These phylogenies imply that a separate genus is necessary, and Connopus is proposed. Infraspecific morphological and DNA-based variation within C. acervatus suggests that a western North American clade might be reproductively isolated from the eastern North American/Scandinavian clade and that in this species complex the European and eastern North American clade might be conspecific. A Scandinavian exemplar is selected for bar-coding. Two GenBank sequences with name-phylogenetic placement inconsistencies are identified.


The Coconut Palm, Cocos Nucifera, Impacts Forest Composition And Soil Characteristics At Palmyra Atoll, Central Pacific, Hillary S. Young, Ted K. Raab, Douglas J. McCauley, Amy A. Briggs, Rodolfo Dirzo 2009 Stanford University

The Coconut Palm, Cocos Nucifera, Impacts Forest Composition And Soil Characteristics At Palmyra Atoll, Central Pacific, Hillary S. Young, Ted K. Raab, Douglas J. Mccauley, Amy A. Briggs, Rodolfo Dirzo

Ted K. Raab

Cocos nucifera, the coconut palm, has a pantropical distribution and reaches near monodominance in many atolls, low lying islands and coastal regions. This paper examines the ecological correlation between C. nucifera abundance and changes in forest structure, floristic diversity and forest soil characteristics. Cumulatively, these data show that C. nucifera has important impacts on floristic, structural and soil characteristics of forests where it becomes dominant. Given the high proportion of tropical coastal areas in which C. nucifera is now naturalized and abundant, this likely has important implications for coastal forest diversity and structure.


An Integrated Modeling Method For Tibia Strain Analysis, Daniel Leib, Henry Wang 2009 Boise State University

An Integrated Modeling Method For Tibia Strain Analysis, Daniel Leib, Henry Wang

Daniel Leib

No abstract provided.


Members’ Discoveries: Fatal Flaws In Cancer Research, Jeffrey S. Morris 2009 The University of Texas M.D. Anderson Cancer Center

Members’ Discoveries: Fatal Flaws In Cancer Research, Jeffrey S. Morris

Jeffrey S. Morris

A recent article published in The Annals of Applied Statistics (AOAS) by two MD Anderson researchers—Keith Baggerly and Kevin Coombes—dissects results from a highly-influential series of medical papers involving genomics-driven personalized cancer therapy, and outlines a series of simple yet fatal flaws that raises serious questions about the veracity of the original results. Having immediate and strong impact, this paper, along with related work, is providing the impetus for new standards of reproducibility in scientific research.


Caffeine Does Not Augment Markers Of Muscle Damage Or Leukocytosis Following Resistance Exercise, Marco Machado, Alexander J. Koch, Jeffrey Willardson, Frederico C. dos Santos, Victor M. Curty, Lucan N. Pereira 2009 Truman State University

Caffeine Does Not Augment Markers Of Muscle Damage Or Leukocytosis Following Resistance Exercise, Marco Machado, Alexander J. Koch, Jeffrey Willardson, Frederico C. Dos Santos, Victor M. Curty, Lucan N. Pereira

Jeffrey Willardson

The purpose of this study was to evaluate the effects of caffeine ingestion before a resistance exercise session on markers of muscle damage (CK, LDH, ALT, AST) and leukocyte levels. Methods: Fifteen soccer athletes completed two resistance exercise sessions that differed only in the ingestion of caffeine or a placebo preworkout. Results: CK concentration increased significantly following the caffeine session (415.8 ± 62.8 to 542.0 ± 73.5) and the placebo session (411.5 ± 43.3 to 545.8 ± 59.9), with no significant differences between sessions. Similarly, LDH concentration increased significantly following the caffeine session (377.5 ± 18 ...


Interference With Histidyl-Trna Synthetase By A Crispr Spacer Sequence As A Factor In The Evolution Of Pelobacter Carbinolicus, Derek Lovley, Muktak Aklujkar 2009 University of Massachusetts - Amherst

Interference With Histidyl-Trna Synthetase By A Crispr Spacer Sequence As A Factor In The Evolution Of Pelobacter Carbinolicus, Derek Lovley, Muktak Aklujkar

Muktak Aklujkar

BACKGROUND: Pelobacter carbinolicus, a bacterium of the family Geobacteraceae, cannot reduce Fe(III) directly or produce electricity like its relatives. How P. carbinolicus evolved is an intriguing problem. The genome of P. carbinolicus contains clustered regularly interspaced short palindromic repeats (CRISPR) separated by unique spacer sequences, which recent studies have shown to produce RNA molecules that interfere with genes containing identical sequences. RESULTS: CRISPR spacer #1, which matches a sequence within hisS, the histidyl-tRNA synthetase gene of P. carbinolicus, was shown to be expressed. Phylogenetic analysis and genetics demonstrated that a gene paralogous to hisS in the genomes of Geobacteraceae ...


Browse Biomass Removal And Nutritional Condition Of Moose Alces Alces, C Tom Seaton, Thomas F. Paragi, Rodney D. Boertje, Knut Kielland, Stephen DuBois, Craig L. Fleener 2009 University of Alaska, Fairbanks

Browse Biomass Removal And Nutritional Condition Of Moose Alces Alces, C Tom Seaton, Thomas F. Paragi, Rodney D. Boertje, Knut Kielland, Stephen Dubois, Craig L. Fleener

Craig L Fleener

No abstract provided.


The 1.9 Å Structure Of Human Α-N-Acetylgalactosaminidase The Molecular Basis Of Schindler And Kanzaki Diseases, Nathaniel E. Clark, Scott Garman 2009 University of Massachusetts - Amherst

The 1.9 Å Structure Of Human Α-N-Acetylgalactosaminidase The Molecular Basis Of Schindler And Kanzaki Diseases, Nathaniel E. Clark, Scott Garman

Scott Garman

alpha-N-acetylgalactosaminidase (alpha-NAGAL; E.C. 3.2.1.49) is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalactosamine residues from glycopeptides and glycolipids. In humans, a deficiency of alpha-NAGAL activity results in the lysosomal storage disorders Schindler disease and Kanzaki disease. To better understand the molecular defects in the diseases, we determined the crystal structure of human alpha-NAGAL after expressing wild-type and glycosylation-deficient glycoproteins in recombinant insect cell expression systems. We measured the enzymatic parameters of our purified wild-type and mutant enzymes, establishing their enzymatic equivalence. To investigate the binding specificity and catalytic mechanism of the human alpha-NAGAL enzyme, we determined three ...


Antioxidant And Antibacterial Activities Of Ethanolic Extracts Of Asparagus Officinalis Cv. Mary Washington: Comparison Of In Vivo And In Vitro Grown Plant Bioactivities, Rosna Binti Mat Taha 2009 University of Malaya

Antioxidant And Antibacterial Activities Of Ethanolic Extracts Of Asparagus Officinalis Cv. Mary Washington: Comparison Of In Vivo And In Vitro Grown Plant Bioactivities, Rosna Binti Mat Taha

Rosna Binti Mat Taha

The antioxidant and antibacterial activities of ethanolic extracts of in vivo grown Asparagus officinalis cv. Mary Washington were investigated using superoxide dismutase, erythrocyte haemolysis and 2,2diphenyl-1-picrylhydrazil free radical scavenging methods. The measured antioxidant and antimicrobial potential were then compared to the activities offered by the ethanolic extracts of in vitro grown A. officinalis as well as ethanolic extract of undifferentiated callus cells of A. officinalis produced on Murashige and Skoog medium containing 1.5 mg/l 6-benzylaminopurine combined with 0.5 mg/l naphthalene acetic acid. The highest antioxidant capacity was obtained from the in vivo grown plant extract ...


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