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The Role Of Plasmalogen In The Oxidative Stability Of Neutral Lipids And Phospholipids, Guang Wang, Tong Wang 2010 Iowa State University

The Role Of Plasmalogen In The Oxidative Stability Of Neutral Lipids And Phospholipids, Guang Wang, Tong Wang

Food Science and Human Nutrition Publications

The role of ethanolamine plasmalogen extracted from bovine brain (BBEP) in maintaining oxidative stability of bulk soybean oil and liposome made with egg phospholipids (PL) was studied. In a purified soybean oil (PSO), the addition of 200 and 1000 ppm of BBEP promoted lipid oxidation at rates of 0.037 and 0.071 (all rates in ln (PV) h−1, and PV stands for peroxide value), whereas soy lecithin (SL) added in the same amount showed a trend similar to the PSO blank, which had an oxidation rate of 0.025. The PSO with BBEP was susceptible to cupric ion ...


Rice Expressing Wheat Hppk/Dhps: A Transgenic Cereal Crop With Enhanced Folate, Susan Gillies, Shane McIntosh, Robert Henry 2010 Southern Cross University

Rice Expressing Wheat Hppk/Dhps: A Transgenic Cereal Crop With Enhanced Folate, Susan Gillies, Shane Mcintosh, Robert Henry

Susan Gillies

No abstract provided.


Detecting Mitochondrial Genotypes By Temperature Gradient Gel Electrophoresis And Heteroduplex Analysis, Martin Elphinstone, Peter Baverstock 2010 Southern Cross University

Detecting Mitochondrial Genotypes By Temperature Gradient Gel Electrophoresis And Heteroduplex Analysis, Martin Elphinstone, Peter Baverstock

Martin S Elphinstone

No abstract provided.


An Inexpensive And High-Throughput Procedure To Extract And Purify Total Genomic Dna For Population Studies, Martin Elphinstone, Gavin Hinten, Megan Anderson, Catherine Nock 2010 Southern Cross University

An Inexpensive And High-Throughput Procedure To Extract And Purify Total Genomic Dna For Population Studies, Martin Elphinstone, Gavin Hinten, Megan Anderson, Catherine Nock

Martin S Elphinstone

We describe here a procedure for the purification of high molecular weight genomic DNA that combines the economies of ‘do-it-yourself’, single-tube protocols with the sample throughput and DNA quality of microplate-based DNA extraction and purification kits from commercial suppliers. The procedure allows the preparation of genomic DNA of a quality suitable for polymerase chain reaction-based studies of large populations at around one-tenth of the cost of commercially available kits. Furthermore, 96 samples can be purified from crude tissue digests in around 30 min and are produced in microtitre plate format to allow efficient downstream processing of samples.


Discovery Of Single Nucleotide Polymorphisms In Sugarcane For Gene Mapping Using 454 Sequencing, Peter Bundock, Frances Eliott, Gary Ablett, Adam Benson, Stirling Bowen, Robert Henry 2010 Southern Cross University

Discovery Of Single Nucleotide Polymorphisms In Sugarcane For Gene Mapping Using 454 Sequencing, Peter Bundock, Frances Eliott, Gary Ablett, Adam Benson, Stirling Bowen, Robert Henry

Gary A Ablett

Sugarcane is genetically complex due to polyploidy, aneuploidy and hybridisation which have led to a large but variable number of copies of each chromosome and a large number of chromosomes overall in the genomes of commercial varieties. To map genes of interest, ‘single dose’ SNPs are desirable because they are fully informative across the mapping population. However discovery of these SNPs requires considerable depth of sequencing to find the single copy alleles and confirm the polymorphisms. To achieve this depth, 454 sequencing of pooled PCR amplicons was utilised on the parents of a QTL mapping population. Three hundred pooled amplicons ...


Discovering Snps For Gene Mapping In Sugarcane Using Deep Sequencing, Peter Bundock, Frances Eliott, Gary Ablett, Adam Benson, Rosanne Casu, Karen Aitken, Robert Henry 2010 Southern Cross University

Discovering Snps For Gene Mapping In Sugarcane Using Deep Sequencing, Peter Bundock, Frances Eliott, Gary Ablett, Adam Benson, Rosanne Casu, Karen Aitken, Robert Henry

Gary A Ablett

As part of a marker program for QTL discovery, 313 sugarcane genes were targeted for the development of SNP markers. However, discovering useful SNPs for mapping these candidates from EST sequences in the public domain was found to be inefficient. As an alternative approach we designed primers to amplify regions of more than 200 of these genes for re-sequencing using 454 Life Sciences Genome Sequencer™ FLX. A region of a four gasket 454 sequencing run was used for the pooled amplicons from each of two mapping population parents. The sequencing yielded 96,755 and 86,241 sequences with perfect matches ...


Breeding For Biofuel Traits, Robert Henry, Agnelo Furtado, Nicole Rice 2010 Southern Cross University

Breeding For Biofuel Traits, Robert Henry, Agnelo Furtado, Nicole Rice

Agnelo Furtado

No abstract provided.


Genomics For Cereal Grain Quality Improvement, Agnelo Furtado, Robert Henry 2010 Southern Cross University

Genomics For Cereal Grain Quality Improvement, Agnelo Furtado, Robert Henry

Agnelo Furtado

No abstract provided.


Utility Of Promoter Sequence Variation In Plant Breeding, Agnelo Furtado, Robert Henry 2010 Southern Cross University

Utility Of Promoter Sequence Variation In Plant Breeding, Agnelo Furtado, Robert Henry

Agnelo Furtado

DNA-based markers such as SNP and SSR, rely on the variations in DNA sequence, which in coding sequences can be determined by comparing EST sequences. The public availability of EST sequence data has supported the mining and discovery of a larger number of SNP-based markers. The advent of next generation sequencing (NGS) has accelerated the production of whole genome sequences providing sequence data of coding sequences but also of promoter sequences. The sequence variation within promoter sequences can now be harnessed to develop DNA-based markers for plant breeding. Promoter sequences are crucial for functional genomics where controlled expression of a ...


Automated Detection Of Infectious Disease Outbreaks In Hospitals: A Retrospective Cohort Study, Susan S. Huang, Deborah S. Yokoe, John Stelling, Hilary Placzek, Martin Kulldorff, Ken Kleinman, Thomas F. O'Brien, Michael S. Calderwood, Johanna Vostok, Julie Dunn, Richard Platt 2010 University of California Irvine

Automated Detection Of Infectious Disease Outbreaks In Hospitals: A Retrospective Cohort Study, Susan S. Huang, Deborah S. Yokoe, John Stelling, Hilary Placzek, Martin Kulldorff, Ken Kleinman, Thomas F. O'Brien, Michael S. Calderwood, Johanna Vostok, Julie Dunn, Richard Platt

GSBS Student Publications

BACKGROUND: Detection of outbreaks of hospital-acquired infections is often based on simple rules, such as the occurrence of three new cases of a single pathogen in two weeks on the same ward. These rules typically focus on only a few pathogens, and they do not account for the pathogens' underlying prevalence, the normal random variation in rates, and clusters that may occur beyond a single ward, such as those associated with specialty services. Ideally, outbreak detection programs should evaluate many pathogens, using a wide array of data sources.

METHODS AND FINDINGS: We applied a space-time permutation scan statistic to microbiology ...


Argonautes Alg-3 And Alg-4 Are Required For Spermatogenesis-Specific 26g-Rnas And Thermotolerant Sperm In Caenorhabditis Elegans., Colin C. Conine, Pedro J. Batista, Weifeng Gu, Julie M. Claycomb, Daniel A. Chaves, Masaki Shirayama, Craig C. Mello 2010 University of Massachusetts Medical School

Argonautes Alg-3 And Alg-4 Are Required For Spermatogenesis-Specific 26g-Rnas And Thermotolerant Sperm In Caenorhabditis Elegans., Colin C. Conine, Pedro J. Batista, Weifeng Gu, Julie M. Claycomb, Daniel A. Chaves, Masaki Shirayama, Craig C. Mello

GSBS Student Publications

Gametogenesis is a thermosensitive process in numerous metazoans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute PRG-1, have been implicated in maintaining fertility at elevated temperature. Here we describe the role of two AGO-class paralogs, alg-3 (T22B3.2) and alg-4 (ZK757.3), in promoting thermotolerant male fertility. A rescuing GFP::alg-3 transgene is localized to P granules beginning at the late pachytene stage of male gametogenesis. alg-3/4 double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate ...


The Correlation Between Rates Of Cancer And Autism: An Exploratory Ecological Investigation, Hung-Teh Kao, Stephen L. Buka, Karl T. Kelsey, David F. Gruber, Barbara Porton 2010 Brown University

The Correlation Between Rates Of Cancer And Autism: An Exploratory Ecological Investigation, Hung-Teh Kao, Stephen L. Buka, Karl T. Kelsey, David F. Gruber, Barbara Porton

Publications and Research

Background: Autism is associated with high rates of genomic aberrations, including chromosomal rearrangements and de novo copy-number variations. These observations are reminiscent of cancer, a disease where genomic rearrangements also play a role. We undertook a correlative epidemiological study to explore the possibility that shared risk factors might exist for autism and specific types of cancer.

Methodology/Principal Findings: To determine if significant correlations exist between the prevalence of autism and the incidence of cancer, we obtained and analyzed state-wide data reported by age and gender throughout the United States. Autism data were obtained from the U.S. Department of ...


Analysis Of Primary Risk Factors For Oral Cancer From Us States With Increasing Rates, Anthony Bunnell, Nathan Pettit, Nicole Reddout, Kanika Sharma, Susan O'Malley, Michelle Chino, Karl Kingsley 2010 University of Nevada, Las Vegas

Analysis Of Primary Risk Factors For Oral Cancer From Us States With Increasing Rates, Anthony Bunnell, Nathan Pettit, Nicole Reddout, Kanika Sharma, Susan O'Malley, Michelle Chino, Karl Kingsley

Community Health Sciences Faculty Publications

Objectives

To examine the primary risk factor for oral cancer in the US, smoking and tobacco use, among the specific US states that experienced short-term increases in oral cancer incidence and mortality.

Methods

Population-based data on oral cancer morbidity and mortality in the US were obtained from the National Cancer Institute's (NCI) Surveillance, Epidemiology, and End Results (SEER) database for analysis of recent trends. Data were also obtained from the Centers for Disease Control and Prevention (CDC) Behavioral Risk Factor Surveillance System (BRFSS) to measure current and former trends of tobacco usage. To comprehensive measures of previous state tobacco ...


Iowa Water Conference March 8-9, Brent A. Pringnitz 2010 Iowa State University

Iowa Water Conference March 8-9, Brent A. Pringnitz

Integrated Crop Management News

"Pulling it all together: Policy, programs and practices” is the theme for this year’s Iowa Water Conference hosted by the Iowa Water Center. The conference will feature the Iowa Stormwater Conference, the Agriculture and Environment Conference and the Source Water Protection Conference. This partnership effort strives to advance Iowa urban and agricultural water issues through sustainable watershed management.


The Evolution Of Human Genetic And Phenotypic Variation In Africa, Michael C. Campbell, Sarah A. Tishkoff 2010 Howard University

The Evolution Of Human Genetic And Phenotypic Variation In Africa, Michael C. Campbell, Sarah A. Tishkoff

Michael C. Campbell

Africa is the birthplace of modern humans, and is the source of the geographic expansion of ancestral populations into other regions of the world. Indigenous Africans are characterized by high levels of genetic diversity within and between populations. The pattern of genetic variation in these populations has been shaped by demographic events occurring over the last 200,000 years. The dramatic variation in climate, diet, and exposure to infectious disease across the continent has also resulted in novel genetic and phenotypic adaptations in extant Africans. This review summarizes some recent advances in our understanding of the demographic history and selective ...


Dragnet: Software For Storing, Managing And Analyzing Annotated Draft Genome Sequence Data, Stacy Duncan, Ruchita Sirkanungo, Leslie Miller, Gregory J. Phillips 2010 Iowa State University

Dragnet: Software For Storing, Managing And Analyzing Annotated Draft Genome Sequence Data, Stacy Duncan, Ruchita Sirkanungo, Leslie Miller, Gregory J. Phillips

Veterinary Microbiology and Preventive Medicine Publications

Background
New "next generation" DNA sequencing technologies offer individual researchers the ability to rapidly generate large amounts of genome sequence data at dramatically reduced costs. As a result, a need has arisen for new software tools for storage, management and analysis of genome sequence data. Although bioinformatic tools are available for the analysis and management of genome sequences, limitations still remain. For example, restrictions on the submission of data and use of these tools may be imposed, thereby making them unsuitable for sequencing projects that need to remain in-house or proprietary during their initial stages. Furthermore, the availability and use ...


Bean Leaf Beetle Predictions For 2010, Erin W. Hodgson, Nick Schmidt 2010 Iowa State University

Bean Leaf Beetle Predictions For 2010, Erin W. Hodgson, Nick Schmidt

Integrated Crop Management News

Bean leaf beetles have adapted to Iowa's winters by protecting themselves in leaf litter, but they are still susceptible to cold temperatures. Harsh winters can cause significant mortality. In general, bean leaf beetle adults will die if temperatures fall below -10C. An overwintering model was developed by Lam and Pedigo from Iowa State University in 2000, and is helpful for predicting winter mortality based on accumulating subfreezing temperatures. Figure 1 is a map of predicted mortality in Iowa from the 2009-2010 winter. In general, Iowa experienced very low temperatures, with predicted mortality ranging from 82-99 percent. These numbers are ...


Interview With Darrell Sanders, Cully Community Garden, 2010 (Audio), Darrell Sanders 2010 Portland State University

Interview With Darrell Sanders, Cully Community Garden, 2010 (Audio), Darrell Sanders

All Sustainability History Project Oral Histories

Interview of Darrell Sanders by Jeremy Cohen in Portland, Oregon on February 20th, 2010.

The interview index is available for download.


Lincoln South Rotary Program, February 2010 [Lincoln, Nebraska, Usa]: Global Food Security--Intsormil, Elvis A. Heinrichs 2010 University of Nebraska - Lincoln

Lincoln South Rotary Program, February 2010 [Lincoln, Nebraska, Usa]: Global Food Security--Intsormil, Elvis A. Heinrichs

INTSORMIL Impacts and Bulletins

Short Heinrichs spoke to the Lincoln South Rotary Club in February 2010 about global food security. What is food security? The availability of food and access to it. Many countries have food available but the population has no access to it. Heinrichs gave the group some sobering statistics regarding hunger in the world. Nine hundred forty-seven million people in the world are undernourished. One hundred forty-six billion kids under age 5 are underweight. One in seven people are hungry, and one in six people do not have safe drinking water. Ten million kids die each year from hunger related causes ...


Expanding Ethics Education In Science & Engineering, Jane E. Fountain 2010 University of Massachusetts - Amherst

Expanding Ethics Education In Science & Engineering, Jane E. Fountain

Ethics in Science and Engineering National Clearinghouse

This presentation was part of the AAAS Annual Conference professional development workshop, National Science Foundation and Ethics Education in Science and Engineering, during the recent meeting in San Diego, California (18-22 February). Dr. Michael Gorman, Program Director, Science, Technology & Society, National Science Foundation, moderated the workshop presentations and the discussion that followed. In addition, he contributed a set of powerpoint slides outlining the role of NSF in its response to the America Competes Act, including a commitment to support an online resource in ethics education. Dr. Philip Langlais, Vice Provost for Graduate Studies & Research, Old Dominion University, presented recommendations for ...


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