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Novel Translational Regulation Of The Proapoptotic Bcl2 Member Puma And Its Role During Skeletal Myoblast Apoptosis, Atossa Shaltouki 2011 Cleveland State University

Novel Translational Regulation Of The Proapoptotic Bcl2 Member Puma And Its Role During Skeletal Myoblast Apoptosis, Atossa Shaltouki

ETD Archive

Differentiation and apoptosis are coordinately regulated in skeletal myoblasts. During the process of skeletal myoblast differentiation, our lab has determined that roughly 30 of myoblasts undergo apoptosis rather than differentiation. Further, we have reported that cytochrome C is released, and that the expression level of the pro-apoptotic Bcl2 family member PUMA is elevated, when 23A2 myoblasts are cultured in DM. Our long-term goal is to identify targets for manipulation that would abrogate this apoptosis without affecting differentiation. These findings could be significant to the study of myoblast transfer as a therapeutic approach. The goals of this dissertation are to: 1- ...


The Parasympathetic Nervous System In Human Heart Failure, Jessica A. French 2011 Cleveland State University

The Parasympathetic Nervous System In Human Heart Failure, Jessica A. French

ETD Archive

Heart failure (HF) affects 5.8 million Americans and is characterized by an inability of the heart to pump blood throughout the body. In a non-diseased state, the sympathetic (SNS) and parasympathetic nervous systems (PNS) innervate the heart to regulate rate and force of contraction. Actions of the PNS on the cardiovascular system are mediated via the vagus nerve, releasing acetylcholine which binds muscarinic receptors on cardiomyocytes. The SNS has been found to be overstimulated in HF, with the role of the PNS in HF unclear. We hypothesized that the PNS is dysregulated in HF, resulting in a change of ...


Intricate Rna : Rna Interactions In U12-Dependent Nuclear Pre-Mrna Splicing, Tupa Basu Roy 2011 Cleveland State University

Intricate Rna : Rna Interactions In U12-Dependent Nuclear Pre-Mrna Splicing, Tupa Basu Roy

ETD Archive

Coding regions or exons of most human genes are interrupted by noncoding intervening regions or introns. Removal of nuclear precursor messenger RNA (pre-mRNA) introns by RNA splicing is an essential step in eukaryotic gene expression. Two types of nuclear pre-mRNA introns are known as U2-dependent or major type and U12-dependent or minor type. Nuclear pre-mRNA introns are removed by two distinct sets of ribonucleoprotein complexes or spliceosomes, which are formed by five small nuclear RNAs (snRNAs) for each spliceosome. U6atac and U12 snRNAs are central to U12-dependent spliceosome and play essential roles in the removal of U12-dependent introns. U6atac and ...


Nest-Site Selection, Nocturnal Nest Desertion, And Productivity In A Common Tern (Sterna Hirundo) Colony At Detroit River, Michigan, Gregory J. Norwood 2011 Eastern Michigan University

Nest-Site Selection, Nocturnal Nest Desertion, And Productivity In A Common Tern (Sterna Hirundo) Colony At Detroit River, Michigan, Gregory J. Norwood

Master's Theses and Doctoral Dissertations

Common terns are declining in the Great Lakes region and represent a population relatively distinct from that of eastern North America. On an artificial site, I studied the substrate and vegetation preference and productivity of nesting terns at two scales: general habitat and nest-site. Terns nested later and less successfully in large river rock (13 to 25-cm diameter) compared to more diverse substrate and limestone (2.5-5.0 cm) when it was free of dead vegetation. Within a habitat, terns chose to nest where substrate was most diverse in rock size, soil, and non-vegetative debris. Percent standing cover did not ...


Diet Of Three Mormoopid Bats (Mormoops Blainvillei, Pteronotus Quadridens, And Pteronotus Portoricensis ) On Puerto Rico, Ashley K. Rolfe 2011 Eastern Michigan University

Diet Of Three Mormoopid Bats (Mormoops Blainvillei, Pteronotus Quadridens, And Pteronotus Portoricensis ) On Puerto Rico, Ashley K. Rolfe

Master's Theses and Doctoral Dissertations

This study used visual analysis to determine the percent volume and percent frequency of orders of insects in the guano of the Antillean ghost-faced bat (Mormoops blainvillei), sooty mustached bat (Pteronotus quadridens), and Puerto Rican mustached bat (Pteronotus portoricensis). The most common orders for all three species were Coleoptera, Hymenoptera, and Lepidoptera, although the relative proportions of these orders differed among species. Variation in diet was primarily due to species, but season, habitat, and sex also affected the composition of insects in the feces. In addition, species-level identification of prey was achieved through molecular techniques that examined mitochondrial DNA isolated ...


Factors Associated With Fruit And Vegetable Consumption Among Caribbean Immigrants In The United States, Claudia Martin-Ayoade 2011 Eastern Michigan University

Factors Associated With Fruit And Vegetable Consumption Among Caribbean Immigrants In The United States, Claudia Martin-Ayoade

Master's Theses and Doctoral Dissertations

Background: There are limited data available on the fruit and vegetable consumption of Caribbean immigrants in the United States.

Purpose: To assess changes in intakes of fruits and vegetables in Caribbean immigrants while assessing knowledge of the recommended servings and barriers to intake.

Method: A cross sectional Internet questionnaire was conducted for Caribbean-born members of the social network website Cariblifecentral.com. Data were analyzed using descriptive frequencies, Pearson’s chi-square, and the Student’s t-test.

Results: Of 113 respondents, 37 reported consuming less vegetables since emigrating (p<0.10) while 29 reported eating more. However, 10.8% of respondents did not know the recommended servings for vegetables. Access to fresh produce was no barrier to consumption.

Conclusions: As this population continues to grow, it becomes necessary to tailor nutrition ...


Munterville: A New Soil Series In Iowa, Mostafa A. Ibrahim, C. Lee Burras, Jason Steele, Mark La Van, Michael L. Thompson, Michael Sucik 2011 Ohio State University - Main Campus

Munterville: A New Soil Series In Iowa, Mostafa A. Ibrahim, C. Lee Burras, Jason Steele, Mark La Van, Michael L. Thompson, Michael Sucik

Agronomy Publications

Before 2009, the Natural Resources Conservation Service (NRCS) staff noticed plant roots and argillans deep within the B horizon of Gosport polypedons (a fine, illitic, mesic Oxyaquic Dystrudept) while updating Major Land Resource Area (MLRA) 108 (Illinois and Iowa deep loess and drift) and 109 (Iowa and Missouri heavy till plain) in the central United States. In 2009, Gosport soils in MLRAs 108 and 109 were remapped and reclassified. Both map unit transects and the relevé method were used to identify pedons for detailed laboratory analyses. Eight pedons representing Gosport soils were collected from eight counties in southern Iowa, USA ...


Sulfur Fertilization Response In Iowa Corn Production, John E. Sawyer, Brian J. Lang, Daniel W. Barker 2011 Iowa State University

Sulfur Fertilization Response In Iowa Corn Production, John E. Sawyer, Brian J. Lang, Daniel W. Barker

Agronomy Publications

With the positive results from S fertilization in alfalfa (see related article, page 6), trials were started in 2006 corn fields where early plant growth was exhibiting S deficiency symptoms or where there was expectation of S deficiency. Calcium sulfate (CaSO4•H2O, gypsum) was surface broadcast applied after early corn growth at 40 lb S/A, with a control treatment for comparison. The 40 lb S/A rate was chosen as a non-limiting S rate to maximize any potential yield increase.


Sulfur Emerges As A Nutritional Issue In Iowa Alfalfa Production, John E. Sawyer, Brian J. Lang, Daniel W. Barker 2011 Iowa State University

Sulfur Emerges As A Nutritional Issue In Iowa Alfalfa Production, John E. Sawyer, Brian J. Lang, Daniel W. Barker

Agronomy Publications

Sulfur is often classified as a “secondary” essential element, mainly due to a smaller plant requirement, but also because it is less frequently applied as a fertilizer compared to N, P, and K. This has certainly been the case in Iowa, where research had not documented S deficiency or fertilization need for optimal crop production. However, if deficient, S can have a dramatic effect on plant growth and crop productivity – more than the classification “secondary” would imply.


Gene Expression Patterns Are Correlated With Genomic And Genic Structure In Soybean, Jenna L. Woody, Andrew J. Severin, Yung-Tsi Bolon, Bindu Joseph, Brian W. Diers, Andrew D. Farmer, Nathan Weeks, Gary J. Muehlbauer, Rex T. Nelson, David Grant, James E. Specht, Michelle A. Graham, Steven B. Cannon, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker 2011 Iowa State University

Gene Expression Patterns Are Correlated With Genomic And Genic Structure In Soybean, Jenna L. Woody, Andrew J. Severin, Yung-Tsi Bolon, Bindu Joseph, Brian W. Diers, Andrew D. Farmer, Nathan Weeks, Gary J. Muehlbauer, Rex T. Nelson, David Grant, James E. Specht, Michelle A. Graham, Steven B. Cannon, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker

Agronomy Publications

Studies have indicated that exon and intron size and intergenic distance are correlated with gene expression levels and expression breadth. Previous reports on these correlations in plants and animals have been conflicting. In this study, next-generation sequence data, which has been shown to be more sensitive than previous expression profiling technologies, were generated and analyzed from 14 tissues. Our results revealed a novel dichotomy. At the low expression level, an increase in expression breadth correlated with an increase in transcript size because of an increase in the number of exons and introns. No significant changes in intron or exon sizes ...


The Effects Of Soil Ph On Elymus Repens Growth And Tissue Nutrients, Jack H. Dekker, Heather MacKenzie, Kevin Chandler 2011 Iowa State University

The Effects Of Soil Ph On Elymus Repens Growth And Tissue Nutrients, Jack H. Dekker, Heather Mackenzie, Kevin Chandler

Agronomy Publications

The perennial, graminaceous, plant quackgrass (Elymus repens (L.) Gould) is a serious weed problem. When corn is grown continuously, the high amounts of nitrogen fertilizer applied can alter the pH of the soil, and this decrease in soil pH with time may change the weed spectrum present in corn fields. Studies were conducted to determine the effect of four different pH soils (3.7, 4.3, 5.5, 6.2) on the growth of quackgrass rhizome fragments in terms of biomass accumulation and tissue nutrient content. As the soil pH decreased from 6.2 to 3.7, quackgrass plants accumulated ...


Localization And Characterization Of The Interactions Between Basigin Gene Products And Monocarboxlate Transporters In The Olfactory Bulb Of The Mouse, Paul L. Gambon 2011 University of North Florida

Localization And Characterization Of The Interactions Between Basigin Gene Products And Monocarboxlate Transporters In The Olfactory Bulb Of The Mouse, Paul L. Gambon

UNF Theses and Dissertations

Basigin, the mouse form of the human protein EMMPRIN, is commonly found as a transmembrane homodimer with carboxy termini in the cytosol and extracellular amino-termini. Because of its important role as a cell-to-cell junction molecule, and possible implications for cancer research, a Basigin null mouse was developed in 1996 by Igakura et al., to aid in the study of this protein. Their early research demonstrated that Basigin plays a large role in embryonic development. Mice lacking the Basigin gene are blind from the time of eye opening, and have demonstrated a lack of aversion to offensive odors such as acetic ...


Assessing The Effects Of Pollutant Exposure On Sharks: A Biomarker Approach, Christina J. Walker 2011 University of North Florida

Assessing The Effects Of Pollutant Exposure On Sharks: A Biomarker Approach, Christina J. Walker

UNF Theses and Dissertations

Many hydrophobic environmental pollutants have been shown to bioaccumulate and biomagnify at high levels in sharks due to their high liver lipid content, high trophic level, and life history characteristics. Studies have demonstrated that the levels of pollutants present in shark tissues can not only exceed the recommended levels for human consumption, but that, in some cases, they are also greater than the threshold for physiological effect in other aquatic species. However, few studies to date have investigated the biological effect of environmental exposure to contaminants in sharks. Therefore, the goal of this study was to investigate, through the use ...


Comparison Of Tungsten Carbide And Stainless Steel Ball Bearings For Grinding Single Maize Kernels In A Reciprocating Grinder, Jay Hoch, Adrienne Moran Lauter, M. Paul Scott 2011 Iowa State University

Comparison Of Tungsten Carbide And Stainless Steel Ball Bearings For Grinding Single Maize Kernels In A Reciprocating Grinder, Jay Hoch, Adrienne Moran Lauter, M. Paul Scott

Agronomy Publications

When grinding many corn kernels at once, there are several different options to choose from, but single kernel grinding is limited to few options with relatively low throughput. An attractive alternative is a reciprocating grinder with 3/8” grinding balls and a 4ml polycarbonate vial set. This format allows grinding of 24 samples simultaneously, which is a significant improvement to grinding throughput. A reciprocating grinder like the Talboys HT Homogenizer (Troemner, Thorofare, NJ), operates by violently shaking the 4ml polycarbonate vials containing a bearing and a single kernel. In our experience, stainless steel ball bearings do not lead to a ...


Examination Of The Mitochondrial Health Of The Basigin Null Mouse Retina, Kristine Anne V. Pablo 2011 University of North Florida

Examination Of The Mitochondrial Health Of The Basigin Null Mouse Retina, Kristine Anne V. Pablo

UNF Theses and Dissertations

Basigin gene products are cell adhesion molecules that are expressed by photoreceptor cells, Müller cells and endothelial cells of the mammalian retina. Previous studies have suggested that a lactate shuttle exists between the photoreceptor cells and the Müller cells, with Basigin being an essential component in this shuttle. Deletion of the Basigin gene in mice results in blindness with an eventual retinal degeneration. It was hypothesized that the lactate shuttle between photoreceptors and Müller cells does not form in Basigin null mice and that the blindness is attributed to faulty photoreceptor metabolism. Therefore, the purpose of this study was to ...


Protein Expression Of Hypoxia-Inducible Factor 1-Alpha (Hif-1Α) In Spot (Leiostomus Xanthurus) Under Acute Exposure To Hypoxic Treatments : A Laboratory-Field Comparison Study, Mason James Smith 2011 University of North Florida

Protein Expression Of Hypoxia-Inducible Factor 1-Alpha (Hif-1Α) In Spot (Leiostomus Xanthurus) Under Acute Exposure To Hypoxic Treatments : A Laboratory-Field Comparison Study, Mason James Smith

UNF Theses and Dissertations

Hypoxia in coastal estuaries is a topic of increasing concern, as the magnitude and frequency of hypoxic events have increased over the past several decades. These hypoxic events are highly detrimental to the coastal biota, particulary fish. The hypoxia-inducible factor 1α (HIF-1α) protein was used as a candidate biomarker for deciphering exposure of fish to hypoxic events. In chapter one, Spot (Leiostomus xanthurus) were exposed to three treatment groups of DO (means 7.32 ± 0.18, 5.15 ± .37, and 2.57 ± 0.01 mg Lˉ¹ DO) and sampled (n = 5) at time zero, 36 and 72-h for each treatment ...


Physiology And Biochemistry Of The Tropical Seagrass Thalassia Testudinum In Response To Hypersalinity Stress And Labyrinthula Sp. Infection, Stacey Marie Trevathan-Tackett 2011 University of North Florida

Physiology And Biochemistry Of The Tropical Seagrass Thalassia Testudinum In Response To Hypersalinity Stress And Labyrinthula Sp. Infection, Stacey Marie Trevathan-Tackett

UNF Theses and Dissertations

Seagrass meadows are essential to coastal ecosystems and have experienced declines in abundance due to a series of environmental stressors including elevated salinity and incidence of disease caused by the pathogen Labyrinthula sp. This thesis evaluated the dynamics between T. testudinum and Labyrinthula concerning the impacts of short term elevated salinity stress on the early stages of infection in Thalassia testudinum (Chapter 2) as well as the presence of anti-labyrinthulid secondary metabolites produced by the tropical seagrass (Chapter 3). The results showed that short term exposure to hypersalinity alters some cellular processes but does not necessarily lead to an immediate ...


Maize Transgenes Containing Zein Promoters Are Regulated By Opaque2, Megan N. Schroder, Brice E. Floyd, M. Paul Scott 2011 Iowa State University

Maize Transgenes Containing Zein Promoters Are Regulated By Opaque2, Megan N. Schroder, Brice E. Floyd, M. Paul Scott

Agronomy Publications

Understanding epistatic interactions between transgenes and native genes is critical for predicting the response of transgenes to different genetic backgrounds and environments. Our objective was to determine if predicted epistatic interactions occur between maize (Zea mays L.) transgenes carrying zein promoters and opaque2, a transcriptional regulator of zein genes. Expression of the transgenes was significantly decreased in kernels containing the opaque2 mutation. Native zein proteins in these kernels were reduced as well. Thus, transgene expression mirrored expression of the native gene that contributed the promoter of the transgene. This work demonstrates that information about native gene expression may be useful ...


Resistant Starch And Starch Thermal Characteristics In Exotic Corn Lines Grown In Temperate And Tropical Environments, Linda M. Pollak, M. Paul Scott, Susan A. Duvick 2011 United States Department of Agriculture

Resistant Starch And Starch Thermal Characteristics In Exotic Corn Lines Grown In Temperate And Tropical Environments, Linda M. Pollak, M. Paul Scott, Susan A. Duvick

Agronomy Publications

Corn as a food that is heated and cooled to allow starch retrogradation has higher levels of resistant starch (RS). Increasing the amount of RS can make corn an even healthier food and may be accomplished by breeding and selection, especially by using exotic germplasm. Sixty breeding lines of introgressed exotic germplasm backgrounds, selected for high yield, were grown in three tropical and temperate locations and analyzed for starch thermal characteristics and RS levels. Although actual values for all starch characteristics were within normal levels, most characteristics had significant genotypic effects, and all had significant location effects. Thermal properties of ...


Identification And Characterization Of The Major Outer Membrane Proteins Of Haemophilus Parasuis For The Rational Development Of A Vaccine Candidate And Diagnostic For Glässer's Disease, Mandy Kay Zimmerli 2011 Iowa State University

Identification And Characterization Of The Major Outer Membrane Proteins Of Haemophilus Parasuis For The Rational Development Of A Vaccine Candidate And Diagnostic For Glässer's Disease, Mandy Kay Zimmerli

Graduate Theses and Dissertations

Haemophilus parasuis is the causative agent of Glässer's disease, a respiratory illness in swine, which causes significant economic loss in the industry. To date, there is no commercially available cross-protective vaccine or diagnostic for this disease. Because of this, identification and characterization of putative virulence factors is crucial. In order to understand the mechanism of infection of H. parasuis, it is imperative to not only identify proteins traditionally associated with virulence but to also recognize differences that exist for those proteins at a molecular and/or expression level. Using a combination of bottom-up and isobaric-tagging for relative and absolute ...


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