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The Influence Of Inoculated And Native Ectomycorrhizal Fungi On Morphology, Physiology And Survival Of American Chestnut, Jenise M. Bauman, Carolyn H. Keiffer, Shiv Hiremath 2011 Western Washington University

The Influence Of Inoculated And Native Ectomycorrhizal Fungi On Morphology, Physiology And Survival Of American Chestnut, Jenise M. Bauman, Carolyn H. Keiffer, Shiv Hiremath

Huxley College on the Peninsulas Publications

The objective of this study was to evaluate the influence of five different species of ectomycorrhizal (ECM) fungi on root colonization of native fungi on putatively blight resistant chestnut hybrids (Castanea dentata x C. mollissima) in a reclaimed mine site in central Ohio. The five species were Hebeloma crustuliniforme, Laccaria bicolor, Scleroderma polyrhizum, Amanita rubescens, and Suillus luteus. We used a combination of DNA sequencing of the ITS region and phylogenetic analyses to indentify fungi found on roots after 12 and 18 months in the field. Non-metric multidimensional scaling (NMDS) ordinations were used to determine if ECM community composition was ...


Gordon Natural Area Tour - Guide, Kendra McMillin, Gerard Hertel 2011 West Chester University of Pennsylvania

Gordon Natural Area Tour - Guide, Kendra Mcmillin, Gerard Hertel

Gordon Natural Area-related Curricula Documents

No abstract provided.


Ectomycorrhizal Colonization & Diversity On Red Oaks Are Reduced In Response To Garlic Mustard Density & Extracts, Justin DiPhillippo, Gregory D. Turner 2011 west chester university

Ectomycorrhizal Colonization & Diversity On Red Oaks Are Reduced In Response To Garlic Mustard Density & Extracts, Justin Diphillippo, Gregory D. Turner

Garlic Mustard Study Documents

No abstract provided.


The Green Legacy Project: Evaluating Campus Tree Benefits, Joy Fritschle, Gary Coutu, Joan Welch, Gerard Hertel 2011 West Chester University of Pennsylvania

The Green Legacy Project: Evaluating Campus Tree Benefits, Joy Fritschle, Gary Coutu, Joan Welch, Gerard Hertel

Geography & Planning Faculty Publications

No abstract provided.


Effect Of Substrate Composition And Alignment On Corneal Cell Phenotype, Donna Phu '09, Lindsay S. Wray '08, Robert V. Warren '10, Richard C. Haskell, Elizabeth J. Orwin 2011 Harvey Mudd College

Effect Of Substrate Composition And Alignment On Corneal Cell Phenotype, Donna Phu '09, Lindsay S. Wray '08, Robert V. Warren '10, Richard C. Haskell, Elizabeth J. Orwin

All HMC Faculty Publications and Research

Corneal blindness is a significant problem treated primarily by corneal transplants. Donor tissue supply is low, creating a growing need for an alternative. A tissue-engineered cornea made from patient-derived cells and biopolymer scaffold materials would be widely accessible to all patients and would alleviate the need for donor sources. Previous work in this lab led to a method for electrospinning type I collagen scaffolds for culturing corneal fibroblasts ex vivo that mimics the microenvironment in the native cornea. This electrospun scaffold is composed of small-diameter, aligned collagen fibers. In this study, we investigate the effect of scaffold nanostructure and composition ...


Albumin Precursorandhsp70modulatecornealwoundhealinginanorgan Culturemodel, Anwar Ali Siddiqui Aga Khan University, Shamim Mushtaq Shamim Mushtaq, ZulfiqarAliNaqvi ZulfiqarAliNaqvi, NikhatAhmed NikhatAhmed 2011 AgaKhanUniversity

Albumin Precursorandhsp70modulatecornealwoundhealinginanorgan Culturemodel, Anwar Ali Siddiqui Aga Khan University, Shamim Mushtaq Shamim Mushtaq, Zulfiqaralinaqvi Zulfiqaralinaqvi, Nikhatahmed Nikhatahmed

Department of Biological & Biomedical Sciences

In order to investigate the role of albumin precursor and Hsp70 in corneal wound healing, we have analyzed the distribution of these proteins in wounded and non-wounded corneas of rabbits and the effects of topical applications of anti-albumin precursor and anti-Hsp70 antibodies on wound healing. Anti-albumin precursor and anti-Hsp70 antibodies were topically applied in healing corneal epithelium of rabbit eyes in organ culture. Corneas were allowed to heal in vitro for up to 120 h in serum-free medium with 5 and 10 μg/ml or without (migrating control) anti-albumin precursor/ or anti-Hsp70 antibodies. Fibronectin (Fb) (5 μg/ml) was used ...


William R. Overlease (1925 - 2007), Jack Holt 2011 West Chester University

William R. Overlease (1925 - 2007), Jack Holt

Gordon Natural Area History & Strategic Plan Documents

No abstract provided.


The Jackrabbit Dairy Review, Vikram Mistry 2011 South Dakota State University

The Jackrabbit Dairy Review, Vikram Mistry

The Jackrabbit Dairy Review

No abstract provided.


Electrospinning Fabrication, Structural And Mechanical Characterization Of Rod-Like Virus-Based Composite Nanofibers, L. Wu, J. Zang, L. A. Lee, Z. Niu, G. C. Horvatha, V. Braxtona, A. C. Wibowo, M. A. Bruckman, Soumitra Ghoshroy, Hans Conrad zur Loye, X. Li, Qian Wang 2011 University of South Carolina - Columbia

Electrospinning Fabrication, Structural And Mechanical Characterization Of Rod-Like Virus-Based Composite Nanofibers, L. Wu, J. Zang, L. A. Lee, Z. Niu, G. C. Horvatha, V. Braxtona, A. C. Wibowo, M. A. Bruckman, Soumitra Ghoshroy, Hans Conrad Zur Loye, X. Li, Qian Wang

Faculty Publications

Tobacco mosaic virus (TMV) was electrospun with polyvinyl alcohol (PVA) into continuous TMV–PVA composite nanofibers to form a biodegradable nonwoven fibrous mat as an extracellular matrix (ECM) mimetic. Morphological characterizations by electron microscopyshowed that the addition of varying amounts of TMV resulted in homogeneous nanofibers without phase separation and did not change the diameter of the composite nanofibers. The orientation of TMV in as-spun fibers could be readily controlled and post-processing of the nonwoven TMV–PVA mat significantly improved its water resistance. In addition, tensile tests were performed on individual nanofibers, which revealed that the TMV–PVA composite nanofibers ...


Systematic Approach To Electrostatically Induced 2d Crystallization Of Nanoparticles At Liquid Interfaces, S. Kewalramani, S. Wang, Y. Lin, H. G. Nguyen, Qian Wang, M. Fukuto, L. Yang 2011 University of South Carolina - Columbia

Systematic Approach To Electrostatically Induced 2d Crystallization Of Nanoparticles At Liquid Interfaces, S. Kewalramani, S. Wang, Y. Lin, H. G. Nguyen, Qian Wang, M. Fukuto, L. Yang

Faculty Publications

We report an experimental demonstration of a strategy for inducing two-dimensional (2D)crystallization of charged nanoparticles on oppositely charged fluid interfaces. This strategy aims to maximize the interfacial adsorption of nanoparticles, and hence their lateral packing density, by utilizing a combination of weakly charged particles and a high surface charge density on the planar interface. In order to test this approach, we investigated the assembly of cowpea mosaic virus (CPMV) on positively charged lipid monolayers at the aqueous solution surface, by means of in situX-ray scattering measurements at the liquid–vapor interface. Theassembly was studied as a function of ...


Custom-Molded Foot-Orthosis Intervention And Multi-Segment Medial Foot Kinematics During Walking, Stephen C. Cobb, Laurie L. Tis, Jeffrey T. Johnson, Yang "Tai" Wong, Mark D. Geil 2011 University of Wisconsin - Milwaukee

Custom-Molded Foot-Orthosis Intervention And Multi-Segment Medial Foot Kinematics During Walking, Stephen C. Cobb, Laurie L. Tis, Jeffrey T. Johnson, Yang "Tai" Wong, Mark D. Geil

Kinesiology Faculty Publications

Context: Foot-orthosis (FO) intervention to prevent and treat numerous lower extremity injuries is widely accepted clinically. However, the results of quantitative gait analyses have been equivocal. The foot models used, participants receiving intervention, and orthoses used might contribute to the variability.

Objective: To investigate the effect of a custom-molded FO intervention on multisegment medial foot kinematics during walking in participants with low-mobile foot posture.

Design: Crossover study.

Setting: University biomechanics and ergonomics laboratory. Patients or Other

Participants: Sixteen participants with low-mobile foot posture (7 men, 9 women) were assigned randomly to 1 of 2 FO groups. Intervention(s): After a ...


Serotonergic Lesions Of The Periaqueductal Gray, A Primary Source Of Serotonin To The Nucleus Paragigantocellularis, Facilitate Sexual Behavior In Male Rats, Joseph J. Normandin, Anne Z. Murphy PhD 2011 Georgia State University

Serotonergic Lesions Of The Periaqueductal Gray, A Primary Source Of Serotonin To The Nucleus Paragigantocellularis, Facilitate Sexual Behavior In Male Rats, Joseph J. Normandin, Anne Z. Murphy Phd

Neuroscience Institute Faculty Publications

While selective serotonin reuptake inhibitors (SSRIs) are widely used to treat anxiety and depression, they also produce profound disruptions of sexual function including delayed orgasm/ejaculation. The nucleus paragigantocellularis (nPGi), a primary source of inhibition of ejaculation in male rats, contains receptors for serotonin (5-HT). The ventrolateral periaqueductal gray (vlPAG) provides serotonin to this region, thus providing an anatomical and neurochemical basis for serotonergic regulation of the nPGi. We hypothesize that 5-HT acting at the nPGi could underlie the SSRI-induced inhibition of ejaculation in rodents. To this end, we produced 5-HT lesions of the source of 5-HT to the nPGi ...


Lack Of Processing Of The Expressed Orf1 Gene Product Of Hepatitis E Virus, Suganthi Suppiah, Yumei Zhou, Teryl K. Frey 2011 Georgia State University

Lack Of Processing Of The Expressed Orf1 Gene Product Of Hepatitis E Virus, Suganthi Suppiah, Yumei Zhou, Teryl K. Frey

Biology Faculty Publications

Background: Proteolytic processing is a common mechanism among plus strand RNA viruses and the replicases of all plus strand RNA viruses of animals thus far characterized undergo such processing. The replicase proteins of hepatitis E virus (HEV) are encoded by ORF1. A previous report published by our group [1] provided data that processing potentially occurred when ORF1 (Burma strain; genotype 1) was expressed using a vaccinia virus-based expression system.

Findings: To further test for processing and to rule out artifacts associated with the expression system, ORF1 was re-expressed using a plasmid-based expression vector with the result that the previous processing ...


Carbenoxolone Blocks The Light-Evoked Rise In Intracellular Calcium In Isolated Melanopsin Ganglion Cell Photoreceptors, Jayne R. Bramley, Erin M. Wiles, Patricia J. Sollars, Gary E. Pickard 2011 University of Nebraska-Lincoln

Carbenoxolone Blocks The Light-Evoked Rise In Intracellular Calcium In Isolated Melanopsin Ganglion Cell Photoreceptors, Jayne R. Bramley, Erin M. Wiles, Patricia J. Sollars, Gary E. Pickard

Papers in Veterinary and Biomedical Science

Background: Retinal ganglion cells expressing the photopigment melanopsin are intrinsically photosensitive (ipRGCs). These ganglion cell photoreceptors send axons to several central targets involved in a variety of functions. Within the retina ipRGCs provide excitatory drive to dopaminergic amacrine cells via glutamatergic signals and ipRGCs are coupled to widefield GABAergic amacrine cells via gap junctions. However, the extent to which ipRGCs are coupled to other retinal neurons in the ganglion cell layer via gap junctions is unclear. Carbenoxolone, a widely employed gap junction inhibitor, greatly reduces the number of retinal neurons exhibiting non-rod, non-cone mediated light-evoked Ca2+ signals suggesting extensive ...


Relative Dependence Of Neural Plasticity On Age And Activity In The Bumblebee (Bombus Huntii), Geoffrey Nielsen 2011 University of Puget Sound

Relative Dependence Of Neural Plasticity On Age And Activity In The Bumblebee (Bombus Huntii), Geoffrey Nielsen

Summer Research

Neural Plasticity is one of the most fascinating and complex aspects of neuroscience. Due to the complexity of neural plasticity mechanisms, simple models are used to study neural plasticity. The bumblebee, Bombus huntii provides an ideal model organism with which to study neural plasticity, particularly the way it is affected by age and activity1. The Mushroom Body is the portion of the B. huntii brain that is responsible for higher order sensory integration and experience related learning so it will be the focus of this study.


Visualizing The Correlation Between Human Brain Structure And Function, Douglas Hamilton 2011 University of Puget Sound

Visualizing The Correlation Between Human Brain Structure And Function, Douglas Hamilton

Summer Research

In order to help neuroscience researchers better investigate correlations between human brain structure and function, three visualization techniques were implemented in an existing neuroscience investigation software tool. These techniques aimed to produce effective visualizations of gray matter and white matter in the same virtual scene. The three techniques were: 1) wireframe rendering of the gray matter 2) multiple views of separate data sets with linked vantage point and 3) abstraction of white matter pathways drawn as arcs going outside the gray matter.


The Time Progression Of Bub1 And Cdc2 Activation In Zebrafish (Danio Rerio) During Egg Maturation, Julie Swinson 2011 University of Puget Sound

The Time Progression Of Bub1 And Cdc2 Activation In Zebrafish (Danio Rerio) During Egg Maturation, Julie Swinson

Summer Research

The genes bub1 and cdc20 are involved in regulation of meiotic arrest during maturation of the oocyte (immature egg). Meiotic arrest is important in oogenesis, the development of fertilizable eggs, and allows for identification of any DNA mutations or chromosome assembly errors, thus preventing damaged eggs from being ovulated. This study has examined the expression of spindle assembly checkpoint genes bub1 and cdc20. Oocytes were treated with DHP (hormone to induce maturation) or by nocodazole, a spindle destabilizing agent, and relative amounts of mRNA were measured using RT-PCR. These experiments will establish a timeline of bub1 and cdc20 activation after ...


Determining The Origin Of Changing Β-Catenin Concentrations In Zebrafish Oocytes During Maturation, Nathan Pincus 2011 University of Puget Sound

Determining The Origin Of Changing Β-Catenin Concentrations In Zebrafish Oocytes During Maturation, Nathan Pincus

Summer Research

During maturation, oocytes (immature eggs) progress from prophase I to metaphase II of meiosis, and a multitude of other cellular changes occur. β-catenin is a unique protein as it is involved in cell-cell adhesion when it is bound in plasma membrane complexes, and acts as a transcription factor when freed into the cytoplasm and allowed to move into the nucleus. β-catenin has been found to take part in signaling pathways such as the Wnt/β-catenin pathway, which inhibits β-catenin degradation, and subsequently regulates gene transcription. β-catenin has also been found to exist in distinct molecular forms depending on its role ...


Exploring The Effects Of C-Amendment On The Soil Microbial Community In A Puget Lowland Prairie, Jessica Wong 2011 University of Puget Sound

Exploring The Effects Of C-Amendment On The Soil Microbial Community In A Puget Lowland Prairie, Jessica Wong

Summer Research

Scotch broom is a non-native plant that has invaded plant communities worldwide. Able to grow on a variety of soil conditions, Scotch broom associates with soil bacteria to fix nitrogen (N) from the atmosphere, elevating soil N and crowding out native plant species by encouraging the growth of non-natives like itself. Adding carbon (C) has been used in previous studies to lower soil N and restore native plant growth. However, little is known about C-amendment’s effects on the soil microbial community that correspond to changes in soil N and plant community composition. Recent studies have shown that C-amendment increases ...


Methods Of Protein Characterization, Michael Tieu 2011 University of Puget Sound

Methods Of Protein Characterization, Michael Tieu

Summer Research

AppA is a protein in Rhodobacter sphaeroides that has been the topic of debate among scientists for the past several years with regards to the structure of the protein. It has been known that AppA has an effect on the activity of PpsR, which controls the gene expression of photosystems. There are two conflicting experimental structures (2IYG and 1YRX) of the protein, both of which claim to be taken in the dark phase (meaning when there is no light shining on the protein). The debate is about whether some slight differences in the structures represent the shift from the dark ...


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