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Effect Of Drought On Resin Production In Woody Stems Of Rhus Ovata, Sydney T. Adams, Melody Young, Moriah Hall 2017 Pepperdine University

Effect Of Drought On Resin Production In Woody Stems Of Rhus Ovata, Sydney T. Adams, Melody Young, Moriah Hall

Seaver College Research And Scholarly Achievement Symposium

Resin production in stems of woody plants is generated from resin canal secretions and is thought to play a significant role in protection from fungal infection and spread. This may be one of several factors associated with fungal infection and fungal induced dieback in plants during severe water stress or drought. We set out to determine if reduction in resin production in a chaparral shrub, Sugar Bush (Rhus ovata), was associated with severe drought in southern California. We hypothesized that dehydrated R. ovata would produce less resin than hydrated plants. Our null hypothesis was that there is no significant difference ...


Plant Water Relations During Drought-Induced Dieback In Greenbark Ceanothus, Ceanothus Spinosus, In The Santa Monica Mountains, Christopher F. Ross, Andrew Palaski 2017 Pepperdine University

Plant Water Relations During Drought-Induced Dieback In Greenbark Ceanothus, Ceanothus Spinosus, In The Santa Monica Mountains, Christopher F. Ross, Andrew Palaski

Seaver College Research And Scholarly Achievement Symposium

This experiment investigated Ceanothus spinosus dieback in the natural landscape of Pepperdine University’s campus in Malibu, California. We measured midday water potential, gas-exchange rates (photosynthesis and transpiration), chlorophyll fluorescence, and native embolism levels of stem xylem, comparing irrigated controls on campus to plants undergoing severe water stress in the natural landscape of our campus. The water potentials for naturally occurring C. spinosus (-6.6 MPa) was much lower than for irrigated controls (-3.4 MPa). Water stressed plants experience 47.2% embolism in their stem xylem that blocks water transport to leaves compared to 12.6% embolism in irrigated ...


Progression Of Activity And Predator Avoidance In California Grunion Larvae, Callyn E. Shelley 2017 Pepperdine University

Progression Of Activity And Predator Avoidance In California Grunion Larvae, Callyn E. Shelley

Seaver College Research And Scholarly Achievement Symposium

No abstract provided.


15. Enumeration And Gene Identification Of Extended-Spectrum-Beta-Lactamase-Producing Enterobacteriaceae From Impacted Urban Streams In Hall County, Georgia, Usa., Monica Leavell 2017 University of North Georgia

15. Enumeration And Gene Identification Of Extended-Spectrum-Beta-Lactamase-Producing Enterobacteriaceae From Impacted Urban Streams In Hall County, Georgia, Usa., Monica Leavell

University of North Georgia Annual Research Conference

Extended-spectrum beta-lactamases (ESBLs) are a group of enzymes shown to rapidly evolve and confer resistance to a number of antibiotics, including beta lactams. Organisms that produce ESBLs pose both threats and challenges in the administration of appropriate agents to treat infections. ESBLs exhibit antibiotic resistance by destructing the antibiotics’ structure and are typically found encoded on bacterial plasmids that can easily be transferred between bacteria from the family Enterobacteriaceae. Water environments such as streams can help the spread of antibiotic-resistant bacteria which can originate from a variety of sources, including wastewater treatment plants, agricultural sources, and residential septic tank systems ...


A Sumo-Targeted Ubiquitin Ligase Is Involved In The Degradation Of The Nuclear Pool Of The Sumo E3 Ligase Siz1, Oliver Kerscher, Jason W. Westerbeck, Nagesh Pasupala, Mark Guillotte, Eva Szymanski, Brooke C. Matson, Cecilia Esteban 2017 College of William and Mary

A Sumo-Targeted Ubiquitin Ligase Is Involved In The Degradation Of The Nuclear Pool Of The Sumo E3 Ligase Siz1, Oliver Kerscher, Jason W. Westerbeck, Nagesh Pasupala, Mark Guillotte, Eva Szymanski, Brooke C. Matson, Cecilia Esteban

Oliver Kerscher

The Slx5/Slx8 heterodimer constitutes a SUMO-targeted ubiquitin ligase (STUbL) with an important role in SUMO-targeted degradation and SUMO-dependent signaling. This STUbL relies on SUMO-interacting motifs in Slx5 to aid in substrate targeting and carboxy-terminal RING domains in both Slx5 and Slx8 for substrate ubiquitylation. In budding yeast cells, Slx5 resides in the nucleus, forms distinct foci, and can associate with double-stranded DNA breaks. However, it remains unclear how STUbLs interact with other proteins and their substrates. To examine the targeting and functions of the Slx5/Slx8 STUbL, we constructed and analyzed truncations of the Slx5 protein. Our structure–function ...


Budding Yeast Protein Extraction And Purification For The Study Of Function, Interactions, And Post-Translational Modifications, Eva P. Szymanski, Oliver Kerscher 2017 College of William and Mary

Budding Yeast Protein Extraction And Purification For The Study Of Function, Interactions, And Post-Translational Modifications, Eva P. Szymanski, Oliver Kerscher

Oliver Kerscher

Homogenization by bead beating is a fast and efficient way to release DNA, RNA, proteins, and metabolites from budding yeast cells, which are notoriously hard to disrupt. Here we describe the use of a bead mill homogenizer for the extraction of proteins into buffers optimized to maintain the functions, interactions and post-translational modifications of proteins. Logarithmically growing cells expressing the protein of interest are grown in a liquid growth media of choice. The growth media may be supplemented with reagents to induce protein expression from inducible promoters (e.g. galactose), synchronize cell cycle stage (e.g. nocodazole), or inhibit proteasome ...


Sumo-Targeted Ubiquitin Ligase (Stubl) Slx5 Regulates Proteolysis Of Centromeric Histone H3 Variant Cse4 And Prevents Its Mislocalization To Euchromatin, Kentaro Ohkuni, Yoshimitsu Takahashi, Alyona Fulp, Josh Lawrimore, Wei-Chun Au, Nagesh Pasupala, Reuben Levy-Myers, Jack Warren, Alexander Strunnikov, Richard E. Baker, Oliver Kerscher, Kerry Bloom, Munira A. Basrai 2017 National Cancer Institute

Sumo-Targeted Ubiquitin Ligase (Stubl) Slx5 Regulates Proteolysis Of Centromeric Histone H3 Variant Cse4 And Prevents Its Mislocalization To Euchromatin, Kentaro Ohkuni, Yoshimitsu Takahashi, Alyona Fulp, Josh Lawrimore, Wei-Chun Au, Nagesh Pasupala, Reuben Levy-Myers, Jack Warren, Alexander Strunnikov, Richard E. Baker, Oliver Kerscher, Kerry Bloom, Munira A. Basrai

Oliver Kerscher

Centromeric histone H3, CENP-ACse4, is essential for faithful chromosome segregation. Stringent regulation of cellular levels of CENP-ACse4 restricts its localization to centromeres. Mislocalization of CENP-ACse4 is associated with aneuploidy in yeast, flies and tumorigenesis in human cells; thus, defining pathways that regulate CENP-A levels is critical for understanding how mislocalization of CENP-A contributes to aneuploidy in human cancers. Previous work in budding yeast has shown that ubiquitination of overexpressed Cse4 by Psh1, an E3 ligase, partially contributes to proteolysis of Cse4. Here, we provide the first evidence that Cse4 is sumoylated by E3 ligases Siz1 and Siz2 in vivo and ...


Tim18p Is A New Component Of The Tim54p-Tim22p Translocon In The Mitochondrial Inner Membrane, Oliver Kerscher, Naresh B. Sepuri, Robert E. Jensen 2017 College of William and Mary

Tim18p Is A New Component Of The Tim54p-Tim22p Translocon In The Mitochondrial Inner Membrane, Oliver Kerscher, Naresh B. Sepuri, Robert E. Jensen

Oliver Kerscher

The mitochondrial inner membrane contains two separate translocons: one required for the translocation of matrix-targeted proteins (the Tim23p-Tim17p complex) and one for the insertion of polytopic proteins into the mitochondrial inner membrane (the Tim54p-Tim22p complex). To identify new members of the Tim54p-Tim22p complex, we screened for high-copy suppressors of the temperature-sensitivetim54-1 mutant. We identified a new gene,TIM18, that encodes an integral protein of the inner membrane. The following genetic and biochemical observations suggest that the Tim18 protein is part of the Tim54p-Tim22p complex in the inner membrane: multiple copies of TIM18 suppress thetim54-1 growth defect; thetim18::HIS3 disruption is ...


Recognizing Chromosomes In Trouble: Association Of The Spindle Checkpoint Protein Bub3p With Altered Kinetochores And A Unique Defective Centromere, Oliver Kerscher, Luciana B. Crotti, Munira A. Basrai 2017 College of William and Mary

Recognizing Chromosomes In Trouble: Association Of The Spindle Checkpoint Protein Bub3p With Altered Kinetochores And A Unique Defective Centromere, Oliver Kerscher, Luciana B. Crotti, Munira A. Basrai

Oliver Kerscher

Spindle checkpoint proteins monitor the interaction of the spindle apparatus with the kinetochores, halting anaphase even if the microtubule attachment of only a single chromosome is altered. In this study, we show that Bub3p of Saccharomyces cerevisiae, an evolutionarily conserved spindle checkpoint protein, exhibits distinct interactions with an altered or defective kinetochore(s). We show for the first time that green fluorescent protein-tagged S. cerevisiae Bub3p (Bub3-GFP) exhibits not only a diffuse nuclear localization pattern but also forms distinct nuclear foci in unperturbed growing and G2/M-arrested cells. As Bub3-GFP foci overlap only a subset of kinetochores, we tested ...


Sumo-Targeted Ubiquitin Ligase (Stubl) Slx5 Regulates Proteolysis Of Centromeric Histone H3 Variant Cse4 And Prevents Its Mislocalization To Euchromatin, Kentaro Ohkuni, Yoshimitsu Takahashi, Alyona Flup, Josh La, Wei-Chun Au, Nagesh Pasupala, Ruben Levy-Meyers, Jack Warren, Alexander Strunnikov, Richard E. Baker, Oliver Kerscher, Kerry Bloom, Munira A. Basrai 2017 College of William and Mary

Sumo-Targeted Ubiquitin Ligase (Stubl) Slx5 Regulates Proteolysis Of Centromeric Histone H3 Variant Cse4 And Prevents Its Mislocalization To Euchromatin, Kentaro Ohkuni, Yoshimitsu Takahashi, Alyona Flup, Josh La, Wei-Chun Au, Nagesh Pasupala, Ruben Levy-Meyers, Jack Warren, Alexander Strunnikov, Richard E. Baker, Oliver Kerscher, Kerry Bloom, Munira A. Basrai

Oliver Kerscher

Centromeric histone H3, CENP-ACse4, is essential for faithful chromosome segregation. Stringent regulation of cellular levels of CENP-ACse4 restricts its localization to centromeres. Mislocalization of CENP-ACse4 is associated with aneuploidy in yeast and flies and tumorigenesis in human cells; thus defining pathways that regulate CENP-A levels is critical for understanding how mislocalization of CENP-A contributes to aneuploidy in human cancers. Previous work in budding yeast shows that ubiquitination of overexpressed Cse4 by Psh1, an E3 ligase, partially contributes to proteolysis of Cse4. Here we provide the first evidence that Cse4 is sumoylated by E3 ligases Siz1 and Siz2 in vivo and ...


The Yeast Hex3·Slx8 Heterodimer Is A Ubiquitin Ligase Stimulated By Substrate Sumoylation, Yang Xie, Oliver Kerscher, Mary B. Kroetz, Heather F. McConchie, Patrick Sung, Mark Hochstrasser 2017 College of William and Mary

The Yeast Hex3·Slx8 Heterodimer Is A Ubiquitin Ligase Stimulated By Substrate Sumoylation, Yang Xie, Oliver Kerscher, Mary B. Kroetz, Heather F. Mcconchie, Patrick Sung, Mark Hochstrasser

Oliver Kerscher

Hex3 and Slx8 are Saccharomyces cerevisiae proteins with important functions in DNA damage control and maintenance of genomic stability. Both proteins have RING domains at their C termini. Such domains are common in ubiquitin and ubiquitin-like protein ligases (E3s), but little was known about the molecular functions of either protein. In this study we identified HEX3 as a high-copy suppressor of a temperature-sensitive small ubiquitin-related modifier (SUMO) protease mutant, ulp1ts, suggesting that it may affect cellular SUMO dynamics. Remarkably, even a complete deletion of ULP1 is strongly suppressed. Hex3 forms a heterodimer with Slx8. We found that the Hex3·Slx8 ...


The Yeast Nuclear Pore Complex Functionally Interacts With Components Of The Spindle Assembly Checkpoint, Tatiana Louk, Oliver Kerscher, Robert J. Scott, Munira A. Basrai, Richard W. Wozniak 2017 College of William and Mary

The Yeast Nuclear Pore Complex Functionally Interacts With Components Of The Spindle Assembly Checkpoint, Tatiana Louk, Oliver Kerscher, Robert J. Scott, Munira A. Basrai, Richard W. Wozniak

Oliver Kerscher

Aphysical and functional link between the nuclear pore complex (NPC) and the spindle checkpoint machinery has been established in the yeast Saccharomyces cerevisiae. We show that two proteins required for the execution of the spindle checkpoint, Mad1p and Mad2p, reside predominantly at the NPC throughout the cell cycle. There they are associated with a subcomplex of nucleoporins containing Nup53p, Nup170p, and Nup157p. The association of the Mad1p–Mad2p complex with the NPC requires Mad1p and is mediated in part by Nup53p. On activation of the spindle checkpoint, we detect changes in the interactions between these proteins, including the release of ...


A Sumo-Targeted Ubiquitin Ligase Is Involved In The Degradation Of The Nuclear Pool Of The Sumo E3 Ligase Siz1, Jason W. Westerbeck, Nagesh Pasupala, Mark Guillotte, Eva Szymanski, Brooke C. Matson, Cecilia Esteban, Oliver Kerscher 2017 College of William and Mary

A Sumo-Targeted Ubiquitin Ligase Is Involved In The Degradation Of The Nuclear Pool Of The Sumo E3 Ligase Siz1, Jason W. Westerbeck, Nagesh Pasupala, Mark Guillotte, Eva Szymanski, Brooke C. Matson, Cecilia Esteban, Oliver Kerscher

Oliver Kerscher

The Slx5/Slx8 heterodimer constitutes a SUMO-targeted ubiquitin ligase (STUbL) with an important role in SUMO-targeted degradation and SUMO-dependent signaling. This STUbL relies on SUMO-interacting motifs in Slx5 to aid in substrate targeting and carboxy-terminal RING domains in both Slx5 and Slx8 for substrate ubiquitylation. In budding yeast cells, Slx5 resides in the nucleus, forms distinct foci, and can associate with double-stranded DNA breaks. However, it remains unclear how STUbLs interact with other proteins and their substrates. To examine the targeting and functions of the Slx5/Slx8 STUbL, we constructed and analyzed truncations of the Slx5 protein. Our structure–function ...


Science 108-001 & 002 Essentials Of Forensic Science Course And Assignment Schedule Spring 2015, Christina Beatty 2017 Parkland College

Science 108-001 & 002 Essentials Of Forensic Science Course And Assignment Schedule Spring 2015, Christina Beatty

Christina Beatty

No abstract provided.


Science 108-001 Essentials Of Forensic Science Fall 2015, Christina Beatty 2017 Parkland College

Science 108-001 Essentials Of Forensic Science Fall 2015, Christina Beatty

Christina Beatty

No abstract provided.


Creating A Culture Of Evidence-Based Teaching Through Faculty Professional Development, Kirsten Fleming, David Polcyn 2017 California State University - San Bernardino

Creating A Culture Of Evidence-Based Teaching Through Faculty Professional Development, Kirsten Fleming, David Polcyn

Academic Chairpersons Conference Proceedings

This session will focus on leading change designed to instill a culture of equity-minded, evidence-based teaching and learning. Drawing on a community of practice model, a variety of professional development opportunities will be explored that enable STEM faculty members to redesign courses in ways that intentionally incorporates evidence-based teaching practices.


Evaluating Workload: A Collaborative Approach Between Dean And Department Chair, Melissa J. Marcucci Ph.D., Sandra G. Affenito Ph.D., R.D. FAND 2017 University of Saint Joseph

Evaluating Workload: A Collaborative Approach Between Dean And Department Chair, Melissa J. Marcucci Ph.D., Sandra G. Affenito Ph.D., R.D. Fand

Academic Chairpersons Conference Proceedings

Faculty workload calculations are complex and an institution’s mission directs the level of faculty participation in each teaching, scholarship and service. The University of Saint Joseph (USJ; West Hartford, Connecticut) transitioned from a college to a university, necessitating reexamination of faculty workload policy. The review was conducted collaboratively between senior administration and key faculty to understand best practices in calculating teaching workload. A review of best practice, evaluation of workload policies at a defined set of peer institutions, and an internal investigation of the types of course offered by USJ faculty was examined.


A Flexible, Incentivized Budgeting System For Academic Departments, N. Douglas Lees 2017 Indiana University - Purdue University Indianapolis

A Flexible, Incentivized Budgeting System For Academic Departments, N. Douglas Lees

Academic Chairpersons Conference Proceedings

This session will involve a discussion of the budgeting models of the attendees and a discussion of their overall needs for budgeting guidance. An overview of Responsibility Centered Management (RCM) will be provided along with the model used to set budgets for departments in a School of Science.


Working With Students And External Constituents To Revitalize An Undergraduate Degree Program, Anthony S. Overton 2017 Alabama A&M University

Working With Students And External Constituents To Revitalize An Undergraduate Degree Program, Anthony S. Overton

Academic Chairpersons Conference Proceedings

Offering undergraduate programs which are relevant and provide students excellent opportunities for employment or graduate work are key for program success and program growth. The session will describe our approach to revitalizing an unpopular undergraduate degree program based on student input and collaboration with industry and a community college.


Developing And Supporting The Diversity Of Chairperson Roles, Jean Pawl, Richard Griner, Deborah Richardson, Elizabeth Nesmith 2017 Augusta University

Developing And Supporting The Diversity Of Chairperson Roles, Jean Pawl, Richard Griner, Deborah Richardson, Elizabeth Nesmith

Academic Chairpersons Conference Proceedings

A monthly Chair Professional Development program at Augusta University provides opportunity to mentor new chairpersons and foster collegiality amongst all chairpersons at a university with both liberal arts and health sciences programs. The merits and challenges of this program that addresses needs of such a diverse group will be highlighted


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