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Molecular, Genetic, and Biochemical Nutrition Commons

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The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Dwight Forshee 2018 Olivet Nazarene University

The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Dwight Forshee

ELAIA

The microbiome is a dynamic community that can positively and negatively influence host health. Lactobacillus reuteri is a probiotic that has received much attention for its ability to inhibit pathogens such as Salmonella typhimurium, Escherichia coli, and Clostridium difficile. It does so by its unique ability to metabolize glycerol into the antimicrobial compound 3-HPA, which is commonly referred to as reuterin. The ability to secrete reuterin is dependent not only on glycerol availability but also the concentration of glucose. In fact, there appears to be a “goldilocks” ratio between glucose and glycerol as either too much or too little glucose ...


Reporter Bacteriophage T7nlc Utilizes A Novel Nanoluc::Cbm Fusion For The Ultrasensitive Detection Of Escherichia Coli In Water., T.C. Hinkley, S. Garing, S. Singh, A-L. M. Le Ny, K.P. Nichols, J.E. Peters, Joey N. Talbert, S.R. Nugen 2018 Cornell University

Reporter Bacteriophage T7nlc Utilizes A Novel Nanoluc::Cbm Fusion For The Ultrasensitive Detection Of Escherichia Coli In Water., T.C. Hinkley, S. Garing, S. Singh, A-L. M. Le Ny, K.P. Nichols, J.E. Peters, Joey N. Talbert, S.R. Nugen

Food Science and Human Nutrition Publications

Rapid detection of bacteria responsible for foodborne diseases is a growing necessity for public health. Reporter bacteriophages (phages) are robust biorecognition elements uniquely suited for the rapid and sensitive detection of bacterial species. The advantages of phages include their host specificity, ability to distinguish viable and non-viable cells, low cost, and ease of genetic engineering. Upon infection with reporter phages, target bacteria express reporter enzymes encoded within the phage genome. In this study, the T7 coliphage was genetically engineered to express the newly developed luceriferase, NanoLuc (NLuc), as an indicator of bacterial contamination. While several genetic approaches were employed to ...


Biology Of Exosomes And Their Microrna Cargos In Human And Bovine Milk, Amy Lynn Leiferman 2018 University of Nebraska-Lincoln

Biology Of Exosomes And Their Microrna Cargos In Human And Bovine Milk, Amy Lynn Leiferman

Public Access Theses and Dissertations from the College of Education and Human Sciences

Exosomes are small, cargo-containing vesicles secreted by cells to facilitate intercellular communication. Of exosome cargos, microRNAs are especially interesting because of their involvement in gene regulation. Recently, our lab has shown that exosomes and their microRNA cargo are absorbed through the diet and elicit effects exogenously. Human and animal milk contain exosomes, which may have implications in infant and adult nutrition. There is evidence that bovine milk exosomes enhance growth of murine C2C12 myotube cell cultures, but whether this translates to muscle in vivo is unknown. The USDA National Nutrient Database for Standard Reference is lacking up-to-date information about human ...


Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender 2018 Fordham University

Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender

Student Theses 2015-Present

This paper aims to shed light on the dissonance caused by the superimposition of Dominant Human Systems on Natural Systems. I highlight the synthetic nature of Dominant Human Systems as egoic and linguistic phenomenon manufactured by a mere portion of the human population, which renders them inherently oppressive unto peoples and landscapes whose wisdom were barred from the design process. In pursuing a radical pragmatic approach to mending the simultaneous oppression and destruction of the human being and the earth, I highlight the necessity of minimizing entropic chaos caused by excess energy expenditure, an essential feature of systems that aim ...


Pkm2 Influences The Metabolic Fate Of Butyrate In Colorectal Cancer Cells, Megan Louise Pence 2018 University of Tennessee, Knoxville

Pkm2 Influences The Metabolic Fate Of Butyrate In Colorectal Cancer Cells, Megan Louise Pence

University of Tennessee Honors Thesis Projects

No abstract provided.


The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Forshee 2018 Olivet Nazarene University

The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Forshee

Honors Program Projects

The microbiome is a dynamic community that can positively and negatively influence host health. Lactobacillus reuteri is a probiotic that has received much attention for its ability to inhibit pathogens such as Salmonella Typhimurium, Escherichia coli, and Clostridium difficile. It does so by its unique ability to metabolize glycerol into the antimicrobial compound 3-HPA, which is commonly referred to as reuterin. The ability to secrete reuterin is dependent not only on glycerol availability but also the concentration of glucose. In fact, there appears to be a “goldilocks” ratio between glucose and glycerol as either too much or too little glucose ...


Effects Of Mitochondrial Nadp+-Dependent Isocitrate Dehydrogenase Deficiency On Fructose-Induced Obesity In Mice, Allison Michelle Montalbano, Kaleigh Elizabeth Beane 2018 University of Arkansas, Fayetteville

Effects Of Mitochondrial Nadp+-Dependent Isocitrate Dehydrogenase Deficiency On Fructose-Induced Obesity In Mice, Allison Michelle Montalbano, Kaleigh Elizabeth Beane

Human Nutrition and Hospitality Innovation Undergraduate Honors Theses

Obesity prevalence in the United States continues to increase and is associated with health consequences such as type 2 diabetes, hypertension, atherosclerosis, and hyperlipidemia. Among many contributing factors to obesity, fructose may be one of the major reasons as it disrupts the antioxidant system thereby resulting in an accumulation of reactive oxidative species and leading to obese conditions. The enzyme, isocitrate dehydrogenase 2 (IDH2), reduces nicotinamide adenine dinucleotide phosphate from the TCA Cycle, hence might be implicated with not only energy metabolism but also cellular redox homeostasis. Therefore, the hypothesis was that IDH2 deficiency in mice would exacerbate hepatic lipid ...


The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Forshee 2018 Olivet Nazarene University

The Effects Of Inulin And Galactooligosaccharides On The Production Of Reuterin By Lactobacillus Reuteri, Micah Forshee

Scholar Week 2016 - present

The microbiome is a dynamic community that can positively and negatively influence host health. Lactobacillus reuteri is a probiotic that has received much attention for its ability to inhibit pathogens such as Salmonella Typhimurium, Escherichia coli, and Clostridium difficile. It does so by its unique ability to metabolize glycerol into the antimicrobial compound 3-HPA, which is commonly referred to as reuterin. The ability to secrete reuterin is dependent not only on glycerol availability but also the concentration of glucose. In fact, there appears to be a “goldilocks” ratio between glucose and glycerol as either too much or too little glucose ...


Eating Crickets? Nutrient Mineral Content Of Two Cricket Species And Chapul Cricket Protein Bars Using Mp-Aes, Nathan Hopkins 2018 Lynchburg College

Eating Crickets? Nutrient Mineral Content Of Two Cricket Species And Chapul Cricket Protein Bars Using Mp-Aes, Nathan Hopkins

Student Scholar Showcase

Global agriculture is beset by numerous challenges which must be surmounted to feed a developing world, from combating climate change to the cultivation of sustainable food products. This, in addition to protein and nutrient deficiencies in the developing world, makes considering alternative protein sources, such as a crickets, an attractive option. Crickets use a fraction of the land and water that protein sources like beef cattle do, and can be cultivated in urban environments in stacked boxes. This research investigation aims to quantify the nutrient mineral content (Iron, Sodium, Potassium, Calcium, and Magnesium) of two cricket species (Acheta domesticus & Gryllodes ...


Impact Of Bodyweight On Tissue-Specific Folate Status, Genome Wide And Gene-Specific Dna Methylation In Normal Breast Tissues From Premenopausal Women, Armina-Lyn Frederick 2018 University of Massachusetts Amherst

Impact Of Bodyweight On Tissue-Specific Folate Status, Genome Wide And Gene-Specific Dna Methylation In Normal Breast Tissues From Premenopausal Women, Armina-Lyn Frederick

Masters Theses

Obesity has reached an epidemic level in the United States. A number of epidemiological studies have established obesity as a critical risk factor for postmenopausal breast cancer (post-BC), whereas a reverse association holds prior to menopause. A significant scientific gap exists in understanding the mechanism(s) underpinning this epidemiological phenomenon, particularly the reverse association between obesity and premenopausal breast cancer (pre-BC). This study aimed to understand how folate metabolism and DNA methylation informs the association between obesity and pre-BC. Fifty normal breast tissue samples were collected from premenopausal women who underwent reduction mammoplasty. We developed and measured the breast tissue ...


Health Disparities Score Composite Of Youth And Parent Dyads From An Obesity Prevention Intervention: Icook 4-H, Melissa D. Olfert, Makenzie L. Barr, Rebecca L. Hagedorn, Lisa Franzen-Castle, Sarah E. Colby, Kendra K. Kattelmann, Adrienne A. White 2018 West Virginia University

Health Disparities Score Composite Of Youth And Parent Dyads From An Obesity Prevention Intervention: Icook 4-H, Melissa D. Olfert, Makenzie L. Barr, Rebecca L. Hagedorn, Lisa Franzen-Castle, Sarah E. Colby, Kendra K. Kattelmann, Adrienne A. White

Nutrition and Health Sciences -- Faculty Publications

iCook 4-H is a lifestyle intervention to improve diet, physical activity and mealtime behavior. Control and treatment dyads (adult primary meal preparer and a 9–10-year-old youth) completed surveys at baseline and 4, 12, and 24 months. A Health Disparity (HD) score composite was developed utilizing a series of 12 questions (maximum score = 12 with a higher score indicating a more severe health disparity). Questions came from the USDA short form U.S. Household Food Security Survey (5), participation in food assistance programs (1), food behavior (2), level of adult education completed (1), marital status (1), and race (1 adult ...


Inhibitory Effects Of Toll-Like Receptor 4, Nlrp3 Inflammasome, And Interleukin-1Β On White Adipocyte Browning, Meshail Okla, Walid Zaher, Musaad Alfayez, Soonkyu Chung 2018 King Saud University

Inhibitory Effects Of Toll-Like Receptor 4, Nlrp3 Inflammasome, And Interleukin-1Β On White Adipocyte Browning, Meshail Okla, Walid Zaher, Musaad Alfayez, Soonkyu Chung

Nutrition and Health Sciences -- Faculty Publications

Adipose tissue expansion is accompanied by infiltration and accumulation of pro-inflammatory macrophages, which links obesity to pathologic conditions such as type 2 diabetes. However, little is known regarding the role of pro-inflammatory adipose tissue remodeling in the thermogenic activation of brown/beige fat. Here, we investigated the effect of pattern recognition receptors (PRR) activation in macrophages, especially the toll-like receptor 4 (TLR4) and Nod-like receptor 3 (NLRP3), on white adipocyte browning. We report that TLR4 activation by lipopolysaccharide repressed white adipocyte browning in response to β3-adrenergic receptor activation and caused ROS production and mitochondrial dysfunction, while genetic deletion of TLR4 ...


Teens Implementing A Childhood Obesity Prevention Program In The Community: Feasibility And Perceptions Of A Partnership With Hsta And Icook 4-H, Rebecca L. Hagedorn, Jade A. White, Lisa Franzen-Castle, Sarah E. Colby, Kendra K. Kattelmann, Adrienne A. White, Melissa D. Olfert 2018 West Virginia University

Teens Implementing A Childhood Obesity Prevention Program In The Community: Feasibility And Perceptions Of A Partnership With Hsta And Icook 4-H, Rebecca L. Hagedorn, Jade A. White, Lisa Franzen-Castle, Sarah E. Colby, Kendra K. Kattelmann, Adrienne A. White, Melissa D. Olfert

Nutrition and Health Sciences -- Faculty Publications

High school student researchers and teen leaders from the Health Science Technology Academy (HSTA), under the supervision of HSTA teachers, led a childhood obesity prevention (COP) program (iCook 4-H). The objective was to evaluate the feasibility and perceptions of having teen leaders implement a COP program for dyads of youth (9–10 years old) and their primary adult food preparer. Behavior change and perceptions were assessed through surveys and open-ended interviews. Across eight HSTA organizations, 43 teen leaders participated in teaching the iCook 4-H program to 24 dyads. Increased frequency of culinary skills, physical activity and mealtime behavior were reported ...


Milk Exosomes Are Bioavailable And Distinct Microrna Cargos Have Unique Tissue Distribution Patterns, Sonia Manca, Bijaya Upadhyaya, Ezra Mutai, Amy T. Desaulniers, Rebecca A. Cederberg, Brett R. White, Janos Zempleni 2018 University of Nebraska - Lincoln

Milk Exosomes Are Bioavailable And Distinct Microrna Cargos Have Unique Tissue Distribution Patterns, Sonia Manca, Bijaya Upadhyaya, Ezra Mutai, Amy T. Desaulniers, Rebecca A. Cederberg, Brett R. White, Janos Zempleni

Nutrition and Health Sciences -- Faculty Publications

Exosomes participate in cell-to-cell communication, facilitated by the transfer of RNAs, proteins and lipids from donor to recipient cells. Exosomes and their RNA cargos do not exclusively originate from endogenous synthesis but may also be obtained from dietary sources such as the inter-species transfer of exosomes and RNAs in bovine milk to humans. Here, we assessed the bioavailability and distribution of exosomes and their microRNA cargos from bovine, porcine and murine milk within and across species boundaries. Milk exosomes labeled with fluorophores or fluorescent fusion proteins accumulated in liver, spleen and brain following suckling, oral gavage and intravenous administration in ...


Voices For Food: Methodologies For Implementing A Multi-State Community-Based Intervention In Rural, High Poverty Communities, Suzanne Stluka, Lindsay Moore, Heather A. Eicher-Miller, Lisa Franzen-Castle, Becky Henne, Donna Mehrle, Daniel Remley, Lacey McCormack 2018 South Dakota State University

Voices For Food: Methodologies For Implementing A Multi-State Community-Based Intervention In Rural, High Poverty Communities, Suzanne Stluka, Lindsay Moore, Heather A. Eicher-Miller, Lisa Franzen-Castle, Becky Henne, Donna Mehrle, Daniel Remley, Lacey Mccormack

Nutrition and Health Sciences -- Faculty Publications

Background: Rural communities experience unique barriers to food access when compared to urban areas and food security is a public health issue in rural, high poverty communities. A multi-leveled socio-ecological intervention to develop food policy councils (FPCs), and improve food security in rural communities was created. Methods to carry out such an intervention were developed and are described.

Methods: A longitudinal, matched treatment and comparison study was conducted in 24 rural, high poverty counties in South Dakota, Indiana, Missouri, Michigan, Nebraska and Ohio. Counties were assigned to a treatment (n = 12) or comparison (n = 12) group. Intervention activities focus on ...


Epigenetic Modification Of Vitamin D-Induced Gene Expression In Human Colorectal And Breast Cancer Cell Lines, Sharmin Hossain 2018 University of Massachusetts Amherst

Epigenetic Modification Of Vitamin D-Induced Gene Expression In Human Colorectal And Breast Cancer Cell Lines, Sharmin Hossain

Doctoral Dissertations

Epigenetics is the study of the regulation of genes that is not dependent on nucleotide sequence. This may include heritable changes in gene activity and expression but also those that are not heritable. In recent years, epigenetic researchers have made great strides in understanding the molecular mechanisms behind cellular functions in response to diet, exercise, and other lifestyle modifications, like methylation, histone modification etc. It is now known that the epigenome is critical to healthy human development in addition to genetics, and dietary factors can modulate epigenetic alterations in cells. The classic view of cancer etiology is that genetic alterations ...


Discovery Of Bioactive Natural Products From Sugarcane, Rory Patrick Taylor 2018 Southern Cross University

Discovery Of Bioactive Natural Products From Sugarcane, Rory Patrick Taylor

Theses

This research was undertaken to discover new potential products (with a focus on flavonoids) from sugarcane and sugar production by-products that have pharmaceutical, nutraceutical or functional food applications. Specifically, cytotoxic, antioxidant, anti-inflammatory/immune modulation and glycaemic index (GI) activities were surveyed. The results suggest that there are potentially novel anti-inflammatory and α-amylase inhibiting compound(s) present. These extracts or compounds may have use in the treatment of diabetes mellitus type 2 or inflammation-related diseases such as arthritis, multiple sclerosis and atherosclerosis. Further research such as compound identification and in vivo testing is warranted.


Daily Dietary Intake Patterns Improve After Visiting A Food Pantry Among Food-Insecure Rural Midwestern Adults, Breanne N. Wright, Regan L. Bailey, Bruce A. Craig, Richard D. Mattes, Lacey McCormack, Suzanne Stluka, Lisa Franzen-Castle, Becky Henne, Donna Mehrle, Dan Remley, Heather A. Eicher-Miller 2018 Purdue University

Daily Dietary Intake Patterns Improve After Visiting A Food Pantry Among Food-Insecure Rural Midwestern Adults, Breanne N. Wright, Regan L. Bailey, Bruce A. Craig, Richard D. Mattes, Lacey Mccormack, Suzanne Stluka, Lisa Franzen-Castle, Becky Henne, Donna Mehrle, Dan Remley, Heather A. Eicher-Miller

Nutrition and Health Sciences -- Faculty Publications

Emergency food pantries provide food at no cost to low-resource populations. The purpose of this study was to evaluate single-day dietary intake patterns before and after visiting a food pantry among food-secure and food-insecure pantry clients. This observational cohort study comprised a paired, before-and-after design with a pantry visit as the intervention. Participants (n = 455) completed a demographic and food security assessment, and two 24-h dietary recalls. Adult food security was measured using the U.S. Household Food Security Survey Module. Dietary intake patterns were assessed using Automated Self-Administered 24-h Recall data and classified by Healthy Eating Index (HEI-2010) scores ...


Co-Activation, Estimated Anterior And Posterior Cruciate Ligament Forces, And Motor Unit Activation Strategies During The Time Course Of Fatigue, Cory M. Smith, Terry J. Housh, Ethan C. Hill, Josh L. Keller, Glen O. Johnson, Richard J. Schmidt 2018 University of Nebraska - Lincoln

Co-Activation, Estimated Anterior And Posterior Cruciate Ligament Forces, And Motor Unit Activation Strategies During The Time Course Of Fatigue, Cory M. Smith, Terry J. Housh, Ethan C. Hill, Josh L. Keller, Glen O. Johnson, Richard J. Schmidt

Nutrition and Health Sciences -- Faculty Publications

This study aimed to combine co-activation as well as anterior and posterior cruciate ligament force estimations with the motor unit activation strategies employed by the primary muscles that are involved in the movement at the knee joint. Fourteen male subject performed 25 maximal concentric isokinetic leg extension muscle actions at 120 s-1. Electromyographic and mechanomyographic signals from the vastus lateralis and bicep femoris, as well as force, were used to measure co-activation, and estimated anterior and posterior ligament forces during the time course of fatigue. There were decreases in quadriceps force and increases in hamstring force during the 25 ...


Characteristics, Functional Properties, And Antioxidant Activities Of Water-Soluble Proteins Extracted From Grasshoppers, Patanga Succincta And Chondracris Roseapbrunner, Niphattha Chatsuwan, Sitthipong Nalinanon, Yuporn Puechkamut, Buddhi P. Lamsal, Praphan Pinsirodom 2018 King Mongkut’s Institute of Technology Ladkrabang

Characteristics, Functional Properties, And Antioxidant Activities Of Water-Soluble Proteins Extracted From Grasshoppers, Patanga Succincta And Chondracris Roseapbrunner, Niphattha Chatsuwan, Sitthipong Nalinanon, Yuporn Puechkamut, Buddhi P. Lamsal, Praphan Pinsirodom

Food Science and Human Nutrition Publications

Water-soluble proteins extracted from two species of grasshoppers, Patanga succincta (WSPP) and Chondracris roseapbrunner (WSPC), were characterized as well as their functional properties and antioxidant activities were investigated. The extraction yield, on a wet weight basis, was 7.35% and 7.46% for WSPP and WSPC, respectively. The most abundant amino acid in both proteins was glutamic acid, followed by aspartic, alanine, and leucine, in that order. The electrophoretic study revealed that proteins with MW of 29, 42, 50, 69, and 146 kDa were the major protein components in WSPP and WSPC. FTIR analysis showed that those proteins remained their ...


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