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Hla-Do Modulates The Diversity Of The Mhc-Ii Self-Peptidome, Padma P. Nanaware, Mollie M. Jurewicz, John D. Leszyk, Scott A. Shaffer, Lawrence J. Stern 2018 University of Massachusetts Medical School

Hla-Do Modulates The Diversity Of The Mhc-Ii Self-Peptidome, Padma P. Nanaware, Mollie M. Jurewicz, John D. Leszyk, Scott A. Shaffer, Lawrence J. Stern

Open Access Articles

Presentation of antigenic peptides on MHC-II molecules is essential for tolerance to self and for initiation of immune responses against foreign antigens. DO (HLA-DO in humans, H2-O in mice) is a non-classical MHC-II protein that has been implicated in control of autoimmunity and regulation of neutralizing antibody responses to viruses. These effects likely are related to a role of DO in selecting MHC-II epitopes, but previous studies examining the effect of DO on presentation of selected CD4 T cell epitopes have been contradictory. To understand how DO modulates MHC-II antigen presentation, we characterized the full spectrum of peptides presented by ...


Dmk_Dissertation_11.19.18.Pdf, Dana King 2018 Washington University in St. Louis

Dmk_Dissertation_11.19.18.Pdf, Dana King

Dana King

No abstract provided.


Dmk_Dissertation_11.19.18.Pdf, Dana King 2018 Washington University in St. Louis

Dmk_Dissertation_11.19.18.Pdf, Dana King

Dana King

No abstract provided.


Nmecas9 Is An Intrinsically High-Fidelity Genome-Editing Platform, Nadia Amrani, Xin D. Gao, Pengpeng Liu, Alireza Edraki, Aamir Mir, Raed Ibraheim, Ankit Gupta, Kanae E. Sasaki, Tong Wu, Thomas G. Fazzio, Lihua Julie Zhu, Scot A. Wolfe, Erik J. Sontheimer 2018 University of Massachusetts Medical School

Nmecas9 Is An Intrinsically High-Fidelity Genome-Editing Platform, Nadia Amrani, Xin D. Gao, Pengpeng Liu, Alireza Edraki, Aamir Mir, Raed Ibraheim, Ankit Gupta, Kanae E. Sasaki, Tong Wu, Thomas G. Fazzio, Lihua Julie Zhu, Scot A. Wolfe, Erik J. Sontheimer

Open Access Articles

BACKGROUND: The development of CRISPR genome editing has transformed biomedical research. Most applications reported thus far rely upon the Cas9 protein from Streptococcus pyogenes SF370 (SpyCas9). With many RNA guides, wildtype SpyCas9 can induce significant levels of unintended mutations at near-cognate sites, necessitating substantial efforts toward the development of strategies to minimize off-target activity. Although the genome-editing potential of thousands of other Cas9 orthologs remains largely untapped, it is not known how many will require similarly extensive engineering to achieve single-site accuracy within large genomes. In addition to its off-targeting propensity, SpyCas9 is encoded by a relatively large open reading ...


Two Contrasting Classes Of Nucleolus-Associated Domains In Mouse Fibroblast Heterochromatin, Anastassiia Vertii, Jianhong Ou, Jun Yu, Aimin Yan, Hervé Pagès, Haibo Liu, Lihua Julie Zhu, Paul D. Kaufman 2018 University of Massachusetts Medical School

Two Contrasting Classes Of Nucleolus-Associated Domains In Mouse Fibroblast Heterochromatin, Anastassiia Vertii, Jianhong Ou, Jun Yu, Aimin Yan, Hervé Pagès, Haibo Liu, Lihua Julie Zhu, Paul D. Kaufman

University of Massachusetts Medical School Faculty Publications

In interphase eukaryotic cells, almost all heterochromatin is located adjacent to the nucleolus or to the nuclear lamina, thus defining Nucleolus Associated Domains (NADs) and Lamina Associated Domains (LADs), respectively. Here, we determined the first genome-scale map of murine NADs in mouse embryonic fibroblasts (MEFs) via deep sequencing of chromatin associated with purified nucleoli. We developed a Bioconductor package called NADfinder and demonstrated that it identifies NADs more accurately than other peak-calling tools, due to its critical feature of chromosome-level local baseline correction. We detected two distinct classes of NADs. Type I NADs associate frequently with both the nucleolar periphery ...


Dna Subway Purple Line Metagenome Analysis, Ray A. Enke 2018 Selected Works

Dna Subway Purple Line Metagenome Analysis, Ray A. Enke

Ray Enke Ph.D.

This in class walkthrough demonstrates how to use the DNA Subway Purple Line for metagenomics analysis of 16S microbiome Illumina sequencing reads.


Orthogonal Cas9-Cas9 Chimeras Provide A Versatile Platform For Genome Editing, Mehmet F. Bolukbasi, Pengpeng Liu, Kevin Luk, Samantha F. Kwok, Ankit Gupta, Nadia Amrani, Erik J. Sontheimer, Lihua Julie Zhu, Scot A. Wolfe 2018 University of Massachusetts Medical School

Orthogonal Cas9-Cas9 Chimeras Provide A Versatile Platform For Genome Editing, Mehmet F. Bolukbasi, Pengpeng Liu, Kevin Luk, Samantha F. Kwok, Ankit Gupta, Nadia Amrani, Erik J. Sontheimer, Lihua Julie Zhu, Scot A. Wolfe

Open Access Articles

The development of robust, versatile and accurate toolsets is critical to facilitate therapeutic genome editing applications. Here we establish RNA-programmable Cas9-Cas9 chimeras, in single- and dual-nuclease formats, as versatile genome engineering systems. In both of these formats, Cas9-Cas9 fusions display an expanded targeting repertoire and achieve highly specific genome editing. Dual-nuclease Cas9-Cas9 chimeras have distinct advantages over monomeric Cas9s including higher target site activity and the generation of predictable precise deletion products between their target sites. At a therapeutically relevant site within the BCL11A erythroid enhancer, Cas9-Cas9 nucleases produced precise deletions that comprised up to 97% of all sequence alterations ...


20 - Putative Targets Of The Pp2a Serine/Threonine Phosphatase Complex In Regulating Dendritic Morphology, Andre Golshir, Shatabdi Bhattacharjee, Daniel N. Cox 2018 Georgia State University

20 - Putative Targets Of The Pp2a Serine/Threonine Phosphatase Complex In Regulating Dendritic Morphology, Andre Golshir, Shatabdi Bhattacharjee, Daniel N. Cox

Georgia Undergraduate Research Conference (GURC)

Proper dendritic development is essential for functional neuronal networks. Elucidating the molecular mechanisms that regulate dendritic diversification is therefore essential to understanding the formation and modulation of functional neural circuitry. PP2A is a serine/threonine phosphatase that is evolutionarily conserved from yeast to mammals. In Drosophila, the PP2A complex is composed of a catalytic subunit encoded by microtubule star (mts), a scaffolding subunit encoded by PP2A-29B and one of four alternate regulatory subunits encoded by widerborst (wdb), twins, PP2A-B’ and CG4733. Previous studies in the lab showed that specific RNAi-mediated knockdown of various PP2A subunits in CIV neurons severely impaired ...


Alternative Genes For Exploring Ranavirus Phylogenetics: Four Core Genes Compared To The Major Capsid Protein And The Whole Genome., Abigale Garner 2018 Gordon College - Barnesville

Alternative Genes For Exploring Ranavirus Phylogenetics: Four Core Genes Compared To The Major Capsid Protein And The Whole Genome., Abigale Garner

Georgia Undergraduate Research Conference (GURC)

The Iridoviridae is a family of virus that infects invertebrates and poikilothermic vertebrates such as amphibians, reptiles, and fish. Ranavirus is one of the five genera within the Iridoviridae. Ranavirus infections have been seen on every continent. The ranaviruses have 26 core genes, but our study focuses on the utility of: an immediate early protein ICP-46 [open reading frame (ORF) 91R in Frog virus 3 (FV3)]; a transcription elongation factor TIIS (ORF 81R in FV3); Evrl/Air family protein (ORF 88R in FV3); and a hypothetical/putative protein of unknown function (ORF 94R in FV3) as alternative genes for phylogenetic ...


Fastqc Analysis & Hisat Alignments Using Cyverse (Part 2), Ray A. Enke 2018 Selected Works

Fastqc Analysis & Hisat Alignments Using Cyverse (Part 2), Ray A. Enke

Ray Enke Ph.D.

Part 2 of this in class exercise uses CyVerse Discovery Environment (DE) for the following:
  • view the output files of FastQC analysis
  • create custom data tracks from HISAT alignment files for visualization in the UCSC Genome Browser


Fastqc Analysis & Hisat Alignments Using Cyverse (Part 1), Ray A. Enke 2018 Selected Works

Fastqc Analysis & Hisat Alignments Using Cyverse (Part 1), Ray A. Enke

Ray Enke Ph.D.

This in class exercise demonstrates the basic features of the CyVerse Discovery Environment (DE) cyberinfrastructure and also provides a tutorial for setting up FastQC analysis of next generation sequencing reads as well as HISAT alignment of eukaryotic RNA-seq FASTQ files.


Intro To Command Line Coding (Fastqe & Fastp), Ray A. Enke 2018 Selected Works

Intro To Command Line Coding (Fastqe & Fastp), Ray A. Enke

Ray Enke Ph.D.

This in class activity is designed for novices as an introduction to command line coding. The activity uses the programs FASTQE and FASTP to analyze the quality and trim Illumina FASTQ sequencing data.


The Future Of Biodiversity Assessment: Using Environmental Dna And Next-Generation Sequencing To Characterize Biological Communities In The Central Highlands Of Arizona, Courtney Turner-Rathbone 2018 Embry-Riddle Aeronautical University

The Future Of Biodiversity Assessment: Using Environmental Dna And Next-Generation Sequencing To Characterize Biological Communities In The Central Highlands Of Arizona, Courtney Turner-Rathbone

Undergraduate Research Symposium - Prescott

Historically ecological surveys require significant man hours in the field, utilizing methods which can stress species being observed. In using environmental DNA (eDNA) gathered from air, soil, and water, traditional methods may be supplemented or reduced in order to more efficiently utilize funding and reduce or eliminate stress on surveyed species. Water was collected in replicates using aseptic technique from two different sites, 60 miles apart, along the Verde River. Samples were vacuum filtered within 24 hours through 0.45 μm cellulose nitrate (CN) filters to collect eDNA. DNA extraction from the filters was performed. The polymerase chain reaction (PCR ...


Complete Genome Sequences Of 18 Paenibacillus Larvae Phages From The Western United States, Bryan D. Merrill, Christopher P. Fajardo, Jared A. Hilton, Ashley M. Payne, Andy T. Ward, Jamison K. Walker, Aziza Dhalai, Cameron Imahara, James Mangohig, Josh Monk, Cristian Pascacio, Padmani Rai, Alicia Salisbury, Kathie Velez, Travis J. Bloomfield, Brett Buhler, Steven G. Duncan, David A. Fuhriman, Josil George, Kiel Graves, Karli Graves, Hunter L. Hill, Michelle Kim, Bradley K. Knabe, Devin B. Ririe, Spencer L. Rogers, Casey Stamereilers, Michael B. Stephenson, Brittian K. Usher, Colton S. Ward, Jacob M. Withers 2018 Brigham Young University

Complete Genome Sequences Of 18 Paenibacillus Larvae Phages From The Western United States, Bryan D. Merrill, Christopher P. Fajardo, Jared A. Hilton, Ashley M. Payne, Andy T. Ward, Jamison K. Walker, Aziza Dhalai, Cameron Imahara, James Mangohig, Josh Monk, Cristian Pascacio, Padmani Rai, Alicia Salisbury, Kathie Velez, Travis J. Bloomfield, Brett Buhler, Steven G. Duncan, David A. Fuhriman, Josil George, Kiel Graves, Karli Graves, Hunter L. Hill, Michelle Kim, Bradley K. Knabe, Devin B. Ririe, Spencer L. Rogers, Casey Stamereilers, Michael B. Stephenson, Brittian K. Usher, Colton S. Ward, Jacob M. Withers

Life Sciences Faculty Publications

We present here the complete genomes of 18 phages that infect Paenibacillus larvae, the causative agent of American foulbrood in honeybees. The phages were isolated between 2014 and 2016 as part of an undergraduate phage discovery course at Brigham Young University. The phages were isolated primarily from bee debris and lysogens.


Geneweld: A Method For Efficient Targeted Integration Directed By Short Homology, Wesley A. Wierson, Jordan M. Welker, Maira P. Almeida, Carla A. Mann, Dennis A. Webster, Melanie E. Torrie, Trevor J. Weiss, Macy K. Vollbrecht, Merrina Lan, Kenna C. McKeighan, Zhitao MIng, Alec Wehmeier, Christopher S. Mikelson, Jeffrey A. Haltom, Kristen M. Kwan, Chi-­Bin Chien, Darius Balciunas, Stephen C. Ekker, Karl J. Clark, Beau R. Webber, Branden Moriarity, Staci L. Solin, Daniel F. Carlson, Drena L. Dobbs, Maura McGrail, Jeffrey J. Essner 2018 Iowa State University

Geneweld: A Method For Efficient Targeted Integration Directed By Short Homology, Wesley A. Wierson, Jordan M. Welker, Maira P. Almeida, Carla A. Mann, Dennis A. Webster, Melanie E. Torrie, Trevor J. Weiss, Macy K. Vollbrecht, Merrina Lan, Kenna C. Mckeighan, Zhitao Ming, Alec Wehmeier, Christopher S. Mikelson, Jeffrey A. Haltom, Kristen M. Kwan, Chi-­Bin Chien, Darius Balciunas, Stephen C. Ekker, Karl J. Clark, Beau R. Webber, Branden Moriarity, Staci L. Solin, Daniel F. Carlson, Drena L. Dobbs, Maura Mcgrail, Jeffrey J. Essner

Genetics, Development and Cell Biology Publications

Choices for genome engineering and integration involve high efficiency with little or no target specificity or high specificity with low activity. Here, we describe a targeted integration strategy, called GeneWeld, and a vector series for gene tagging, pGTag (plasmids for Gene Tagging), which promote highly efficient and precise targeted integration in zebrafish embryos, pig fibroblasts, and human cells utilizing the CRISPR/Cas9 system. Our work demonstrates that in vivo targeting of a genomic locus of interest with CRISPR/Cas9 and a donor vector containing as little as 24 to 48 base pairs of homology directs precise and efficient knock-in when ...


Complete Genome Sequences Of Paenibacillus Larvae Phages Halcyone, Heath, Scottie, And Unity From Las Vegas, Nevada, Diane G. Yost, Carolyn Chang, Lucy LeBlanc, Erin Cassin, Ceara Peterman, Padmani Rai, Alicia Salisbury, Nicolas Barroga, Ramiro Cisneros, Joseph Fersini, Jonathan Juste, Juvie Ines, Gabriel Leyva, Dyanne Macalinao, Spencer Muscelli, Gustavo S. Reyes, Heather Rhoden, Rodney Tan, Erika Torres, Krystal Tran, Georgette Uriarte-Valle, Christopher Wallace, Simon Wong, Kevin Ayala-Pineda, Vanessa Cadiz, Tiffany Jeanite, Sophia Nhan, Julianne H. Grose, Christy Strong, Penny S. Amy, Philippos K. Tsourkas 2018 University of Nevada, Las Vegas

Complete Genome Sequences Of Paenibacillus Larvae Phages Halcyone, Heath, Scottie, And Unity From Las Vegas, Nevada, Diane G. Yost, Carolyn Chang, Lucy Leblanc, Erin Cassin, Ceara Peterman, Padmani Rai, Alicia Salisbury, Nicolas Barroga, Ramiro Cisneros, Joseph Fersini, Jonathan Juste, Juvie Ines, Gabriel Leyva, Dyanne Macalinao, Spencer Muscelli, Gustavo S. Reyes, Heather Rhoden, Rodney Tan, Erika Torres, Krystal Tran, Georgette Uriarte-Valle, Christopher Wallace, Simon Wong, Kevin Ayala-Pineda, Vanessa Cadiz, Tiffany Jeanite, Sophia Nhan, Julianne H. Grose, Christy Strong, Penny S. Amy, Philippos K. Tsourkas

Life Sciences Faculty Publications

We present the complete genome sequences of four phages that infect Paenibacillus larvae, the causative agent of American foulbrood disease in honeybees. The phages were isolated from beehives and beeswax products from Las Vegas, Nevada. The genomes are 50 to 55 kbp long and use the “direct terminal repeats” DNA-packaging strategy.


Identification And Characterization Of Nitrogen Fixing Bacteria Associated With Kudzu Root Nodules, Chasity Lawless, Michelle Zedonek, Debbie Curtis, Jonda Scharringhausen, Jared Bryant, Wendy A. Dustman, Alexandra M. Kurtz, Bekah Ward Dr. 2018 Georgia Gwinnett College

Identification And Characterization Of Nitrogen Fixing Bacteria Associated With Kudzu Root Nodules, Chasity Lawless, Michelle Zedonek, Debbie Curtis, Jonda Scharringhausen, Jared Bryant, Wendy A. Dustman, Alexandra M. Kurtz, Bekah Ward Dr.

Georgia Journal of Science

Pueraria montana, better known as kudzu, is an invasive species rapidly spreading throughout the southeastern United States. This plant can form root nodules which house nitrogen-fixing bacteria, allowing atmospheric nitrogen to be converted into biologically available forms of nitrogen for use by the plant host. Given the centrality of these bacteria to the spread of kudzu, isolates from nodules were characterized after collection from seven different locations across the metropolitan Atlanta area. Twenty-five isolates were grown on two different variants of nitrogen free media. Four different carbon sources were evaluated as well. Finally, growth under both aerobic and anaerobic conditions ...


Differential Analysis Of Chromatin Accessibility And Histone Modifications For Predicting Mouse Developmental Enhancers, Shaliu Fu, Qin Wang, Jill E. Moore, Michael J. Purcaro, Henry E. Pratt, Kaili Fan, Cuihua Gu, Cizhong Jiang, Ruixin Zhu, Anshul Kundaje, Aiping Lu, Zhiping Weng 2018 Tongji University,China

Differential Analysis Of Chromatin Accessibility And Histone Modifications For Predicting Mouse Developmental Enhancers, Shaliu Fu, Qin Wang, Jill E. Moore, Michael J. Purcaro, Henry E. Pratt, Kaili Fan, Cuihua Gu, Cizhong Jiang, Ruixin Zhu, Anshul Kundaje, Aiping Lu, Zhiping Weng

Program in Bioinformatics and Integrative Biology Publications and Presentations

Enhancers are distal cis-regulatory elements that modulate gene expression. They are depleted of nucleosomes and enriched in specific histone modifications; thus, calling DNase-seq and histone mark ChIP-seq peaks can predict enhancers. We evaluated nine peak-calling algorithms for predicting enhancers validated by transgenic mouse assays. DNase and H3K27ac peaks were consistently more predictive than H3K4me1/2/3 and H3K9ac peaks. DFilter and Hotspot2 were the best DNase peak callers, while HOMER, MUSIC, MACS2, DFilter and F-seq were the best H3K27ac peak callers. We observed that the differential DNase or H3K27ac signals between two distant tissues increased the area under the precision-recall ...


Genetic Architecture Of Gene Expression Traits Across Diverse Populations, Lauren S. Mogil, Angela Andaleon, Alexa Badalamenti, Scott P. Dickinson, Xiuqing Guo, Jerome I. Rotter, W. C. Johnson, Hae Kyung Im, Yongmei Liu, Heather E. Wheeler 2018 Loyola University Chicago

Genetic Architecture Of Gene Expression Traits Across Diverse Populations, Lauren S. Mogil, Angela Andaleon, Alexa Badalamenti, Scott P. Dickinson, Xiuqing Guo, Jerome I. Rotter, W. C. Johnson, Hae Kyung Im, Yongmei Liu, Heather E. Wheeler

Bioinformatics Faculty Publications

For many complex traits, gene regulation is likely to play a crucial mechanistic role. How the genetic architectures of complex traits vary between populations and subsequent effects on genetic prediction are not well understood, in part due to the historical paucity of GWAS in populations of non-European ancestry. We used data from the MESA (Multi-Ethnic Study of Atherosclerosis) cohort to characterize the genetic architecture of gene expression within and between diverse populations. Genotype and monocyte gene expression were available in individuals with African American (AFA, n = 233), Hispanic (HIS, n = 352), and European (CAU, n = 578) ancestry. We performed expression ...


The Transcriptome Of The Bermuda Fireworm Odontosyllis Enopla (Annelida: Syllidae): A Unique Luciferase Gene Family And Putative Epitoky-Related Genes, Mercer R. Brugler, M. Teresa Aguado, Michael Tessler, Mark Siddall 2018 NYC College of Technology (CUNY)

The Transcriptome Of The Bermuda Fireworm Odontosyllis Enopla (Annelida: Syllidae): A Unique Luciferase Gene Family And Putative Epitoky-Related Genes, Mercer R. Brugler, M. Teresa Aguado, Michael Tessler, Mark Siddall

Publications and Research

The Bermuda fireworm Odontosyllis enopla exhibits an extremely tight circalunar circadian behavior that results in an impressive bioluminescent mating swarm, thought to be due to a conventional luciferase-mediated oxidation of a light-emitting luciferin. In addition, the four eyes become hypertrophied and heavily pigmented, and the nephridial system is modified to store and release gametes and associated secretions. In an effort to elucidate transcripts related to bioluminescence, circadian or circalunar periodicity, as well as epitoky-related changes of the eyes and nephridial system, we examined the transcriptomic profile of three female O. enopladuring a bioluminescent swarm in Ferry Reach, Bermuda. Using ...


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