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1,965 full-text articles. Page 77 of 77.

Identification And Characterization Of Oxalate Oxidoreductase, A Novel Thiamine Pyrophosphate-Dependent 2-Oxoacid Oxidoreductase That Enables Anaerobic Growth On Oxalate, Elizabeth Pierce, Donald F. Becker, Stephen W. Ragsdale 2010 University of Michigan, Ann Arbor

Identification And Characterization Of Oxalate Oxidoreductase, A Novel Thiamine Pyrophosphate-Dependent 2-Oxoacid Oxidoreductase That Enables Anaerobic Growth On Oxalate, Elizabeth Pierce, Donald F. Becker, Stephen W. Ragsdale

Biochemistry -- Faculty Publications

Moorella thermoacetica is an anaerobic acetogen, a class of bacteria that is found in the soil, the animal gastrointestinal tract, and the rumen. This organism engages the Wood-Ljungdahl pathway of anaerobic CO2 fixation for heterotrophic or autotrophic growth. This paper describes a novel enzyme, oxalate oxidoreductase (OOR), that enables M. thermoacetica to grow on oxalate, which is produced in soil and is a common component of kidney stones. Exposure to oxalate leads to the induction of three proteins that are subunits of OOR, which oxidizes oxalate coupled to the production of two electrons and CO2 or bicarbonate. Like ...


Identification And Characterization Of Small Compound Inhibitors Of Human Fatp2, Angel Sandoval, Aalap Chokshi, Elliot D. Jesch, Paul N. Black, Concetta C. DiRusso 2010 University of Nebraska-Lincoln

Identification And Characterization Of Small Compound Inhibitors Of Human Fatp2, Angel Sandoval, Aalap Chokshi, Elliot D. Jesch, Paul N. Black, Concetta C. Dirusso

Biochemistry -- Faculty Publications

Fatty acid transport proteins (FATPs) are bifunctional proteins, which transport long chain fatty acids into cells and activate very long chain fatty acids by esterification with coenzyme A. In an effort to understand the linkage between cellular fatty acid transport and the pathology associated with excessive accumulation of exogenous fatty acids, we targeted FATP-mediated fatty acid transport in a high throughput screen of more than 100,000 small diverse chemical compounds in yeast expressing human FATP2 (hsFATP2). Compounds were selected for their ability to depress the transport of the fluorescent long chain fatty acid analogue, C1-BODIPY-C12. Among ...


Formation Of The Redox Cofactor Centers During Cox1 Maturation In Yeast Cytochrome Oxidase, Oleh Khalimonchuk, Megan Bestwick, Brigitte Meunier, Talina C. Watts, Dennis R. Winge 2010 University of Nebraska-Lincoln

Formation Of The Redox Cofactor Centers During Cox1 Maturation In Yeast Cytochrome Oxidase, Oleh Khalimonchuk, Megan Bestwick, Brigitte Meunier, Talina C. Watts, Dennis R. Winge

Biochemistry -- Faculty Publications

The biogenesis of cytochrome c oxidase initiates with synthesis and maturation of the mitochondrionencoded Cox1 subunit prior to the addition of other subunits. Cox1 contains redox cofactors, including the low-spin heme a center and the heterobimetallic heme a3:CuB center. We sought to identify the step in the maturation of Cox1 in which the redox cofactor centers are assembled. Newly synthesized Cox1 is incorporated within one early assembly intermediate containing Mss51 in Saccharomyces cerevisiae. Subsequent Cox1 maturation involves the progression to downstream assembly intermediates involving Coa1 and Shy1. We show that the two heme a cofactor sites in ...


The Role Of Coa2 In Hemylation Of Yeast Cox1 Revealed By Its Genetic Interaction With Cox10, Megan Bestwick, Oleh Khalimonchuk, Fabien Pierrel, Dennis R. Winge 2010 University of Utah Health Sciences Center

The Role Of Coa2 In Hemylation Of Yeast Cox1 Revealed By Its Genetic Interaction With Cox10, Megan Bestwick, Oleh Khalimonchuk, Fabien Pierrel, Dennis R. Winge

Biochemistry -- Faculty Publications

Saccharomyces cerevisiae cells lacking the cytochrome c oxidase (CcO) assembly factor Coa2 are impaired in Cox1 maturation and exhibit a rapid degradation of newly synthesized Cox1. The respiratory deficiency of coa2 Δ cells is suppressed either by the presence of a mutant allele of the Cox10 farnesyl transferase involved in heme a biosynthesis or through impaired proteolysis by the disruption of the mitochondrial Oma1 protease. Cox10 with an N196K substitution functions as a robust gain-of-function suppressor of the respiratory deficiency of coa2 Δ cells but lacks suppressor activity for two other CcO assembly mutant strains, the coa1 Δ and shy1 ...


Quantitative Nuclear Proteomics Identifies Mtor Regulation Of Dna Damage Response, Sricharan Bandhakavi, Young-Mi Kim, Seung-Hyun Ro, Hongwei Xie, Getiria Onsongo, Chang-Bong Jun, Do-Hyung Kim, Timothy J. Griffin 2010 University of Minnesota

Quantitative Nuclear Proteomics Identifies Mtor Regulation Of Dna Damage Response, Sricharan Bandhakavi, Young-Mi Kim, Seung-Hyun Ro, Hongwei Xie, Getiria Onsongo, Chang-Bong Jun, Do-Hyung Kim, Timothy J. Griffin

Biochemistry -- Faculty Publications

Cellular nutritional and energy status regulates a wide range of nuclear processes important for cell growth, survival, and metabolic homeostasis. Mammalian target of rapamycin (mTOR) plays a key role in the cellular responses to nutrients. However, the nuclear processes governed by mTOR have not been clearly defined. Using isobaric peptide tagging coupled with linear ion trap mass spectrometry, we performed quantitative proteomics analysis to identify nuclear processes in human cells under control of mTOR. Within 3 h of inhibiting mTOR with rapamycin in HeLa cells, we observed downregulation of nuclear abundance of many proteins involved in translation and RNA modification ...


Bioelectronics, J. Fleming, Steven Ripp 2009 University of Tennessee - Knoxville

Bioelectronics, J. Fleming, Steven Ripp

Steven Ripp

The Encyclopedia of Industrial Biotechnology was published in order to help readers make sense of the vast amounts of information that have been published around the world across a broad array of ournals, books, and websites. With its comprehensive coverage, Encyclopedia of Industrial Biotechnology is the ideal starting point for research projects involving any aspect of industrial biological processes, including fermentation, biocatalysis, bioseparation, and biofabrication


Bacteriophage Probe-Based Biosensors, M. Zourob, Steven Ripp 2009 University of Tennessee - Knoxville

Bacteriophage Probe-Based Biosensors, M. Zourob, Steven Ripp

Steven Ripp

This book presents a significant and up-to-date review of various recognition receptors, their immobilization, and an overview of surface characterization techniques. Distinguished scientists from key institutions worldwide have contributed chapters that provide a deep analysis of their particular subjects; at the same time, each topic is framed within the context of this integrated approach. This work explores comprehensively the field of recognition receptors, presenting a thorough review of the subject and effective integration of these receptors on sensor surfaces in order to appropriately convey state-of the-art fundamentals and applications of the most innovative approaches.


Bacteriophage-Based Pathogen Detection, Steven Ripp 2009 University of Tennessee - Knoxville

Bacteriophage-Based Pathogen Detection, Steven Ripp

Steven Ripp

Covers trends in modern biotechnology All aspects of this interdisciplinary technology, where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science, are treated


Understanding Stem Cell Research: Controversy And Promise, Father Nicanor Austriaco 2009 Providence College

Understanding Stem Cell Research: Controversy And Promise, Father Nicanor Austriaco

Rev. Nicanor Austriaco, O.P.

What are embryonic stem cells, why are they so exciting and yet controversial, and what can be done to move our society beyond the current moral and political impasse? To provide answers to these questions, we will begin with a basic introduction to the science behind stem cell research. We will then move to a description of the emerging field of regenerative medicine, the main reason why stem cell research has generated so much excitement in the scientific community. As we will see, the drive to develop stem cells for regenerative medicine has linked stem cell research with the controversy ...


What About The Children? Benjamin And Arendt: On Education, Work, And The Political, jules simon 2009 University of Texas at El Paso

What About The Children? Benjamin And Arendt: On Education, Work, And The Political, Jules Simon

Jules Simon

This article is a rough draft of an article that I contributed to an edited volume of articles dealing with progressive education theory. I reflect on articles that Hannah Arendt and Walter Benjamin wrote that deal with educational reform and innovation, both political in nature.


Polyphyly Of The Pikeminnows (Teleostei: Cyprinidae) Inferred Using Mitochondrial Dna Sequences, T. Heath Ogden 2009 Utah Valley University

Polyphyly Of The Pikeminnows (Teleostei: Cyprinidae) Inferred Using Mitochondrial Dna Sequences, T. Heath Ogden

T. Heath Ogden

The phylogenetic relationships of the Colorado pikeminnow Ptychocheilus lucius, northern pikeminnow P. oregonensis, Sacramento pikeminnow P. grandis, Umpqua pikeminnow P. umpquae, and hardhead Mylopharodon conocephalus were examined by using molecular data to investigate monophyly of the genus Ptychocheilus. Phylogenies generated using DNA sequence data from the cytochrome b and 16S ribosomal DNA genes of the mitochondrial genome reveal that Ptychocheilus is a polyphyletic genus and suggest that the taxonomy of the group is in need of further revision. These data yield insights into the evolution of the pikeminnows and help place the significant evolutionary events in context with the geological ...


“El Derecho De La Ue Relativo A Los Organismos Modificados Genéticamente: La Comisión Europea Cambia De Estrategia Para Permitir, Restringir O Prohibir Su Cultivo”, Luis González Vaqué 2009 Asociación Iberoamericana para el Dereho Alimentario

“El Derecho De La Ue Relativo A Los Organismos Modificados Genéticamente: La Comisión Europea Cambia De Estrategia Para Permitir, Restringir O Prohibir Su Cultivo”, Luis González Vaqué

Luis González Vaqué

No abstract provided.


Vertical Dispersal Of Aedes (Stegomyia) Spp. In High-Rise Apartments In Putrajaya, Malaysia, Mohd Sofian Bin Azirun 2009 Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA

Vertical Dispersal Of Aedes (Stegomyia) Spp. In High-Rise Apartments In Putrajaya, Malaysia, Mohd Sofian Bin Azirun

Mohd Sofian Bin Azirun

A preliminary study on the vertical dispersal of Aedes populations in high-rise apartments was carried out in Presint 9, Putrajaya, Malaysia. Ovitraps were placed indoors within four blocks of high-rise apartments from the ground floors (0.0 - 3.0 m) until up to the tenth floors (28.1 - 30.0 m). Aedes aegypti was the dominant species found in the ovitraps (87.85%), while Aedes albopictus was found in lower numbers. From total number of larvae collected (650), 40.92% of these larvae were obtained from the fourth block; Block D. The peak density of Aedes sp. was observed at ...


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