Biochemistry, Biophysics, and Structural Biology Commons^™

Multiple Phase Equilibria In Binary Mixtures Of Phospholipids, Elizabeth Luna, Harden Mcconnell

Elizabeth J. Luna

Approximate phse diagrams describing lateral phase separations are given for binary mixtures of dimyristoyl phosphatidylcholine with dipalmitoyl phosphatidylcholine, distearoyl phosphatidycholine, and dipalmitoyl phosphatidylethanolamine. These diagrams are based in part on freeze-fracture electron microscopic data. These phase diagrams represent an improvement over previous studies in that both solid phses (Pbeta' and Lbeta') of the phosphatidylcholines are included. Further consideration is given to the problem of binary mixtures in which there are two Pbeta' phases that do not form a continuous range of solid solutions.

Bed Bugs Evolved Unique Adaptive Strategy To Resist Pyrethroid Insecticides, Fang Zhu, Hemant Gujar, Jennifer R. Gordon, Kenneth F. Haynes, Michael F. Potter, Subba R. Palli

Entomology Faculty Publications

Recent advances in genomic and post-genomic technologies have facilitated a genome-wide analysis of the insecticide resistance-associated genes in insects. Through bed bug, Cimex lectularius transcriptome analysis, we identified 14 molecular markers associated with pyrethroid resistance. Our studies revealed that most of the resistance-associated genes functioning in diverse mechanisms are expressed in the epidermal layer of the integument, which could prevent or slow down the toxin from reaching the target sites on nerve cells, where an additional layer of resistance (kdr) is possible. This strategy evolved in bed bugs is based on their unique morphological, physiological and behavioral characteristics …

Broad Vision: The Art & Science Of Looking, Heather Barnett, John R. A. Smith

The STEAM Journal

Undergraduate students and academic staff from diverse disciplines in the arts and sciences investigated questions of mediated vision through a year-long interdisciplinary research project at the University of Westminster, London, United Kingdom. The Broad Vision project explored the perception and interpretation of microscopic worlds, and investigated the benefits and challenges of working across disciplinary divides in a university setting. This article describes the three-phase model for interdisciplinary learning and research developed through the project, providing a valuable case study for inquiry based art/science education.

Investigation Of Ionizable Groups That Participate In ᴅ-Arginine Dehydrogenase Catalysis And Binding, Jacob A. Ball

Georgia State Undergraduate Research Conference

No abstract provided.

The Effect Of The R1648h Sodium Channel Mutation On Neuronal Excitability: A Model Study, Christopher Locandro, Robert Clewley

Georgia State Undergraduate Research Conference

No abstract provided.

Probing Manganese (Iii) And (Iv) Porphyrin Complexes With Different Coordination Numbers And Oxidation States By Uv-Vis And 1h-Nmr Spectroscopy, Laren Cyr

Georgia State Undergraduate Research Conference

No abstract provided.

Development Of Novel Inhibitors Targeting Epigenetic Enzymes, Johnny Truong, Leilei Yan Dr., Brandon Canup, Kun Qian, George Y. Zheng Dr.

Georgia State Undergraduate Research Conference

No abstract provided.

Modifying Commercially Available Vectors For Expression Of Standardized Igem Biobricks, Merhawi T. Yigzaw, Dafna Lapido, Holly M. Bowman

Georgia State Undergraduate Research Conference

No abstract provided.

Switching Bistable Leech Heart Interneurons With A Pulse Of Current From Bursting To Silence, Aaron Gomez-Lugo

Georgia State Undergraduate Research Conference

No abstract provided.

Resiliency To Bistability In The Neurons From The Pre-Bötzinger Complex, Treadwell D. Singfield Ii

Georgia State Undergraduate Research Conference

No abstract provided.

Axial Ligands Of The Heme Uptake Protein Hmut From Corynebacterium Diphtheriae, Brandford K. Adobaw, Kimberley Bartlett, Elizabeth Bennett, Dabney Dixon

Georgia State Undergraduate Research Conference

No abstract provided.

Ph And Solvent Viscosity Effects Of ᴅ-Arginine Dehydrogenase With Different ᴅ-Amino Acid Substrates, Quan Bui

Georgia State Undergraduate Research Conference

No abstract provided.

Characterization Of Myxococcus Xanthus Mazf And Implications For A New Point Of Regulation, Tye O. Boynton, Jonathan L. Mcmurry, Lawrence J. Shimkets

Jonathan McMurry

During development, Myxococcus xanthus cells undergo programmed cell death (PCD) whereby 80% of vegetative cells die. Previously, the MazF RNA interferase has been implicated in this role. Recently, it was shown that deletion of the mazF gene does not eliminate PCD in wild-type strain DK1622 as originally seen in DZF1. To clarify the role of MazF, recombinant enzyme was characterized using a highly sensitive assay in the presence and absence of the proposed antitoxin MrpC. In contrast to previous reports that MrpC inhibits MazF activity, the hydrolysis rate was enhanced in a concentration-dependent manner with MrpC or MrpC2, an N-terminally …

A Single Point Mutation In The Listerial Betl Sigma(A)-Dependent Promoter Leads To Improved Osmo- And Chill-Tolerance And A Morphological Shift At Elevated Osmolarity, Ronald F. Hoffman, Susan Mclernon, Audrey Feeney, Colin Hill, Roy D. Sleator

Department of Biological Sciences Publications

Betaine uptake in Listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter BetL. Using a random mutagenesis approach, based on the E. coli XL1 Red mutator strain, we identified a single point mutation in a putative promoter region upstream of the BetL coding region which leads to a significant increase in betL transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress tolerance. Furthermore, the mutation appears to counter the heretofore unreported “twisted” cell morphology observed for L. monocytogenes grown at elevated osmolarities in tryptone soy …

The Effect Of Glycan Linked To Cationic Peanut Peroxidase On Its Activity, Maha M. Al-Subaie

Electronic Thesis and Dissertation Repository

The roles of heme-, calcium- and glycan moieties on the peanut peroxidase enzyme activity have been previously studied. While the 3-D protein structure is known and some information about the glycan chain is known, due to uneven distribution of the 5 glycans, only a partial understanding of the furthest sugar groups on the 16 glycan chain was available. Now, having used gel-filtration column chromatography followed by lectin affinity column work, I showed that there is a rich array of galactose there. Moreover, I determined that not too many of the sugars are removed in the culture period of 14 days. …

Two Biochemically Distinct Lipophosphoglycans From Leishmania Braziliensis And Leishmania Infantum Trigger Different Innate Immune Responses In Murine Macrophages, Izabela Coimbra Ibraim, Rafael Ramiro De Assis, Natália Lima Pessoa, Marco Antônio Campos, Maria Norma Melo, Salvatore Joseph Turco, Rodrigo Pedro Soares

Molecular and Cellular Biochemistry Faculty Publications

BACKGROUND: The dominant, cell surface lipophosphoglycan (LPG) of Leishmania is a multifunctional molecule involved in the interaction with vertebrate and invertebrate hosts. Although the role of LPG on infection has been extensively studied, it is not known if LPG interspecies variations contribute to the different immunopathologies of leishmaniases. To investigate the issue of interspecies polymorphisms, two Leishmania species from the New World that express structural variations of side chains of LPG repeat units were examined. In this context, the procyclic form of L. braziliensis LPG (strain M2903), is devoid of side chains, while the L. infantum LPG (strain BH46) has …

Nuclear Magnetic Resonance Spectroscopic And Computational Investigations Of Chloroperoxidase Catalyzed Regio- And Enantio-Selective Transformations, Rui Zhang

FIU Electronic Theses and Dissertations

Chloroperoxidase (CPO) is the most versatile heme-containing enzyme that catalyzes a broad spectrum of reactions. The remarkable feature of this enzyme is the high regio- and enantio-selectivity exhibited in CPO-catalyzed oxidation reactions. The aim of this dissertation is to elucidate the structural basis for regio- and enantio-selective transformations and investigate the application of CPO in biodegradation of synthetic dyes.

To unravel the mechanism of CPO-catalyzed regioselective oxidation of indole, the dissertation explored the structure of CPO-indole complex using paramagnetic relaxation and molecular modeling. The distances between the protons of indole and the heme iron revealed that the pyrrole ring of …

Hirsutella Sinensis Mycelium Suppresses Interleukin-1b And Interleukin-18 Secretion By Inhibiting Both Canonical And Non-Canonical Inflammasomes, Tsung-Teng Huang, Kowit-Yu Chong, David M. Ojcius, Yi-Hui Wu, Yun-Fei Ko, Cheng-Yeu Wu, Jan Martel, Chia-Chen Lu, Hsin-Chih Lai, John Ding-E. Young

All Dugoni School of Dentistry Faculty Articles

Cordyceps sinensis is a medicinal mushroom used for centuries in Asian countries as a health supplement and tonic. Hirsutella sinensis—the anamorphic, mycelial form of C. sinensis—possesses similar properties, and is increasingly used as a health supplement. Recently, C. sinensis extracts were shown to inhibit the production of the pro-inflammatory cytokine IL-1β in lipopolysaccharide-treated macrophages. However, the molecular mechanism underlying this process has remained unclear. In addition, whether H. sinensis mycelium (HSM) extracts also inhibit the production of IL-1β has not been investigated. In the present study, the HSM extract suppresses IL-1β and IL-18 secretion, and ATP-induced activation of caspase-1. Notably, …

Insights Into The Roles Of Desolvation And Π-Electron Interactions During Dna Polymerization, Edward A. Motea, Irene Lee, Anthony J. Berdis

Chemistry Faculty Publications

This report describes the use of several isosteric non-natural nucleotides as probes to evaluate the roles of nucleobase shape, size, solvation energies, and π-electron interactions as forces influencing key kinetic steps of the DNA polymerization cycle. Results are provided using representative high- and low-fidelity DNA polymerases. Results generated with the E. coli Klenow fragment reveal that this high-fidelity polymerase utilizes hydrophobic nucleotide analogues with higher catalytic efficiencies compared to hydrophilic analogues. These data support a major role for nucleobase desolvation during nucleotide selection and insertion. In contrast, the low-fidelity HIV-1 reverse transcriptase discriminates against hydrophobic analogues and only tolerates non-natural …

Essential Role Of Grim-Led Programmed Cell Death For The Establishment Of Corazonin-Producing Peptidergic Nervous System During Embryogenesis And Metamorphosis In Drosophila Melanogaster, G Lee, R Sehgal, Z Wang, S Nair, K Kikuno, C H. Chen, B Hay, Jae H. Park

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

In Drosophila melanogaster, combinatorial activities of four death genes, head involution defective (hid), reaper (rpr), grim, and sickle (skl), have been known to play crucial roles in the developmentally regulated programmed cell death (PCD) of various tissues. However, different expression patterns of the death genes also suggest distinct functions played by each. During early metamorphosis, a great number of larval neurons unfit for adult life style are removed by PCD. Among them are eight pairs of corazonin-expressing larval peptidergic neurons in the ventral nerve cord (vCrz). To reveal death genes responsible for the PCD of vCrz neurons, we examined extant …