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Effect Of Turmeric On The Promoter Activity Of The Cyp6a8 Gene Of Drosophila Melanogaster, Alexa Stroh 2019 University of Tennessee, Knoxville

Effect Of Turmeric On The Promoter Activity Of The Cyp6a8 Gene Of Drosophila Melanogaster, Alexa Stroh

University of Tennessee Honors Thesis Projects

No abstract provided.


Palmitoylation Of The Sodium Hydrogen Exchanger Isoform 1 (Nhe1): A Novel Post-Translational Protein Modification And Its Regulation Of Nhe1, Emily Pitsch 2018 University of San Diego

Palmitoylation Of The Sodium Hydrogen Exchanger Isoform 1 (Nhe1): A Novel Post-Translational Protein Modification And Its Regulation Of Nhe1, Emily Pitsch

Undergraduate Honors Theses

The sodium-hydrogen exchanger isoform 1 (NHE1) is a ubiquitously expressed transmembrane transporter protein involved in regulating intracellular pH and interacts with a variety of proteins involved in cell signaling, proliferation, and cell motility. The protein has an extended cytosolic tail known as the regulatory region. This project investigates the role of palmitoylation, a reversible fatty acid modification on cysteine amino acids, in the function and behavior of NHE1.


Regulation Of The Pi3-Kinase/Pten Signaling Pathway By Tgf-Β In Prostate Cancer Cells, Mawiyah Kimbrough-Allah 2018 Atlanta University Center

Regulation Of The Pi3-Kinase/Pten Signaling Pathway By Tgf-Β In Prostate Cancer Cells, Mawiyah Kimbrough-Allah

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

Transforming growth factor -β (TGF-β) plays an important role in the progression of prostate cancer. It acts as a tumor suppressor in normal epithelial cells but as a tumor promoter in advanced prostate cancer cells. The PI3-kinase pathway has been shown to play integral roles in many cellular processes including cell proliferation, survival, and cell migration in many cell types. PI3-kinase pathway mediates TGF-β effects on prostate cancer cell migration and invasion. Phosphatase and tensin homolog (PTEN), a tumor suppressor gene, inhibits PI3-kinase pathway and is frequently mutated in prostate cancers. In this present study, we investigated possible roles of ...


Cyclic Urethane: A Versatile Handle For Synthetic Peptide Applications, Hader E. Elashal 2018 Seton Hall University

Cyclic Urethane: A Versatile Handle For Synthetic Peptide Applications, Hader E. Elashal

Seton Hall University Dissertations and Theses (ETDs)

While peptides have impeccable potential due to their biological and therapeutic abilities, they are inherently limited in clinical practice due to their instability towards proteolysis and poor bioavailability. In the past few years peptidomimetics have emerged as a powerful class of compounds that overcomes the shortcomings associated with peptides. Peptidomimetics essentially mimic a natural peptide or protein in its chemical composition, structure and its ability to perform a biologically relevant role. In addition to increased stability and bioavailability, peptidomimetics can also have increased receptor binding affinity and selectivity, thereby making these mimics lead compounds in the field of drug design ...


Development Of Lc-Ms For The Identification And Characterization Of Non-Adjacent Dna Photoproduct Formation In G-Quadruplex Forming Sequences, Claudia Posadas 2018 Washington University in St. Louis

Development Of Lc-Ms For The Identification And Characterization Of Non-Adjacent Dna Photoproduct Formation In G-Quadruplex Forming Sequences, Claudia Posadas

Arts & Sciences Electronic Theses and Dissertations

Ultraviolet light is well known to induce cyclobutane pyrimidine dimers (CPD) and pyrimidine (6–4) pyrimidone photoproducts in duplex DNA, which interfere with DNA replication and transcription. Recently, a new class of DNA photoproducts known as anti cyclobutanepyrimidine dimers have been discovered, which form in G-quadruplex forming sequences in solution. G-quadruplex structures have been proposed to form in human DNA telomeres and certain promoters in vivo but evidence for their existence has been lacking. Since anti-cyclobutante pyrimidine dimers have been shown to form in G-quadruplex forming sequences, their formation in irradiated human cells could be used to confirm the ...


What’S Really Wrong With Medical Research And How To Fix It., Richard Feinman 2018 SUNY Downstate Medical Center

What’S Really Wrong With Medical Research And How To Fix It., Richard Feinman

Journal of Evolution and Health

No abstract provided.


Investigation Into The Amino Acid Characteristic Of Intrinsically Disordered Proteins, Samantha Patterson 2018 University of Wyoming

Investigation Into The Amino Acid Characteristic Of Intrinsically Disordered Proteins, Samantha Patterson

Honors Theses AY 17/18

Caulobacter crescentus, which belongs to the class of Alphaproteobacteria, has a highly organized cytoplasm that includes complex macromolecular structures at the cell poles. These polar complexes are assembled through the activity of a scaffolding protein, known as the Polar Organizing Protein Z (PopZ). PopZ interacts with other proteins via an N-terminal domain that is mostly intrinsically disordered, and contains, an abundance of proline, glutamte, and aspartate (P,E, and D) residues. Surprisingly, scrambling the order of amino acids in the PED region does not affect PopZ function, suggesting that binding affinity and specificity are not dependent on interactions with individual ...


Structure Determination Of A Bioengineered Human/Porcine Factor Viii For Hemophilia A Treatment, And Improvements To The Human Factor Viii Model, Ian Smith 2018 Western Washington University

Structure Determination Of A Bioengineered Human/Porcine Factor Viii For Hemophilia A Treatment, And Improvements To The Human Factor Viii Model, Ian Smith

Graduate Student Symposium

Blood coagulation factor VIII (FVIII), is a non-enzymatic cofactor which plays a crucial role in the formation of a stable blood clot. Absence or deficiency of FVIII results in the blood disorder hemophilia A; with symptoms including internal hemorrhaging and the inability to stop bleeding from open wounds. Treatment of hemophilia A relies on infusions of blood, plasma, or protein concentrates to replace FVIII. Unfortunately, approximately 30% of patients receiving replacement FVIII generate pathologic anti-FVIII inhibitory antibodies, which both reduce the effectiveness of the FVIII therapeutic and increase the severity of hemophilia A symptoms. We have determined the molecular structure ...


Synthetic Routes To Bromo-Terminated Phosphonate Films And Alkynyl Pyridine Compounds For Click Coupling, Poornima Sunder 2018 University of Mary Washington

Synthetic Routes To Bromo-Terminated Phosphonate Films And Alkynyl Pyridine Compounds For Click Coupling, Poornima Sunder

Student Research Submissions

Click reactions are a highly versatile class of reactions that produce a diverse range of products. Copper-catalyzed azide-alkyne cycloaddition (CuAAC) click reactions require an azide and a terminal alkyne and produce a coupled product that is “clicked” through a triazole ring that can have a variety of substituents. In this work, bromo-terminated phosphonate films on copper oxide surfaces were explored as the platform for click coupling, as the terminal azide needed for the reaction can be generated through an in situ SN2 reaction with a terminal bromo group. The reactions were characterized using model reactions in solution before being conducted ...


Structure Of Frequency-Interacting Rna Helicase From Neurospora Crassa Reveals High Flexibility In A Domain Critical For Circadian Rhythm And Rna Surveillance, Yalemi Morales, Keith J. Olsen, Jacqueline M. Bulcher, Sean J. Johnson 2018 Utah State University

Structure Of Frequency-Interacting Rna Helicase From Neurospora Crassa Reveals High Flexibility In A Domain Critical For Circadian Rhythm And Rna Surveillance, Yalemi Morales, Keith J. Olsen, Jacqueline M. Bulcher, Sean J. Johnson

Chemistry and Biochemistry Faculty Publications

The FRH (frequency-interacting RNA helicase) protein is the Neurospora crassa homolog of yeast Mtr4, an essential RNA helicase that plays a central role in RNA metabolism as an activator of the nuclear RNA exosome. FRH is also a required component of the circadian clock, mediating protein interactions that result in the rhythmic repression of gene expression. Here we show that FRH unwinds RNA substrates in vitro with a kinetic profile similar to Mtr4, indicating that while FRH has acquired additional functionality, its core helicase function remains intact. In contrast with the earlier FRH structures, a new crystal form of FRH ...


Engineering The Structure Of The Human Acidic Fibroblast Growth Factor To Enhance Its Stability And Cell Proliferation Activity, Julie Davis Eberle 2018 University of Arkansas, Fayetteville

Engineering The Structure Of The Human Acidic Fibroblast Growth Factor To Enhance Its Stability And Cell Proliferation Activity, Julie Davis Eberle

Theses and Dissertations

Human acidic fibroblast growth factor (hFGF1) is a protein well known for its role in cell growth and differentiation. To elicit these cell-signaling processes, hFGF1 non-selectively binds to any one of the seven cell surface hFGF receptor isoforms. Due to its significant involvement in tissue repair activity, hFGF1 is a prime candidate for novel wound healing therapeutics. However, one drawback toward its use as a novel wound healing therapeutic is the poor inherent thermal stability of hFGF1, as it has been found to unfold near physiological temperature. The cause of this instability is strong electrostatic repulsion created by a dense ...


Optimizing Genetic Manipulation Of Methanogens Through Faster Cloning Techniques, Merrisa Jennings 2018 University of Arkansas, Fayetteville

Optimizing Genetic Manipulation Of Methanogens Through Faster Cloning Techniques, Merrisa Jennings

Biological and Agricultural Engineering Undergraduate Honors Theses

Methanogenesis is the biological production of methane. Only anaerobic archaea known as methanogens are capable of such a metabolic feat. They have strict living conditions and substrate sources which determine their rate of metabolism. This is of particular importance from a greenhouse gas reduction perspective or biogas capturing perspective. One of the best ways to optimize methanogen methane production is via genetic manipulation. The current procedures are timely though, therefore a faster cloning processes should be developed. The objective of this study was to optimize a premade genetic transformation kit known as the Gibson Kit. The Gibson Kit was supposed ...


Efficacy Of Intranasal Administration: A Clinical Approach To Attention, Jacob Feldmann 2018 College of William and Mary

Efficacy Of Intranasal Administration: A Clinical Approach To Attention, Jacob Feldmann

Undergraduate Honors Theses

Orexins are a class of neuropeptides that are associated with homeostatic functions, such as food intake, sexual behavior, and the sleep/wake cycle. Orexins also play a pivotal role in cognitive processes such as attention and memory. In the present experiment, we employed a visual attention task to assess the effects of intranasal administration of orexin A in rats. This task required the rats to discriminate between trials when a visual light was illuminated from trials when the light was not illuminated. We tested the efficacy of this administration technique to see its viability as a therapeutic in attention disorders ...


Functional Studies Of The E. Coli Proc And A Putative Ortholog Mrub_1345, Mourin Azar, Dr. Lori Scott 2018 Augustana College, Rock Island Illinois

Functional Studies Of The E. Coli Proc And A Putative Ortholog Mrub_1345, Mourin Azar, Dr. Lori Scott

Meiothermus ruber Genome Analysis Project

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of Escherichia coli and Meiothermus ruber proC genes using the complementation assay. In this research project, mutants of varying severity to the functional state of the protein were developed. The results showed that two or more amino acid deletions reduced or eliminated ProC function. Amino acid substitutions, on the other hand, were not severe enough to impact ProC function. Double and triple mutants ...


Investigation For Novel Anti-Apoptotic Factors In The Neurons Of Drosophila Melanogaster, Haylie Rachel Lam 2018 University of Tennessee, Knoxville

Investigation For Novel Anti-Apoptotic Factors In The Neurons Of Drosophila Melanogaster, Haylie Rachel Lam

University of Tennessee Honors Thesis Projects

No abstract provided.


Analyzing The Effects Of Site-Directed Mutagenesis On The Structure Of Coxsackievirus B3 Genomic Rna, Erin Dimon 2018 University of Nebraska at Omaha

Analyzing The Effects Of Site-Directed Mutagenesis On The Structure Of Coxsackievirus B3 Genomic Rna, Erin Dimon

Theses/Capstones/Creative Projects

Coxsackievirus B3 (CVB3) is known to cause myocarditis and pancreatitis in humans. The virus has a single stranded RNA genome that codes for 11 different proteins. CVB3 is found to have two serotypes: 28 (virulent and disease causing) and GA (avirulent and not disease causing). Like other members of the Picornaviridae family, CVB3 utilizes the genomic 5’ untranslated region (5’ UTR) to initiate viral replication mechanisms through interactions with host protein factors. Structural variations of the CVB3 5’ UTR are hypothesized to influence the success of such interactions and consequentially determine viral virulence. The aim of this project was to ...


Genetic Testing And A Real World Case Of Lynch Syndrome, Paige Montanaro 2018 University of Rhode Island

Genetic Testing And A Real World Case Of Lynch Syndrome, Paige Montanaro

Senior Honors Projects

In recent years, advancements in genetic testing methods have revolutionized the medical field by enhancing the ability to identify persons with an inherited predisposition to cancer. According to the American Society for Clinical Oncology, individuals should undergo genetic testing when he or she meets the following criteria: the individual demonstrates familial history that indicates a predisposition to certain cancers, the test can be adequately interpreted, and the results will aid in the diagnosis, treatment, or management of the patient or additional family members at risk. Genetic testing can be done on samples of hair, skin, blood, amniotic fluid, or other ...


Design And Development Of A Quartz Crystal Microbalance Immunosensor For Exosomes, Wesley Cox 2018 University of Tennessee, Knoxville

Design And Development Of A Quartz Crystal Microbalance Immunosensor For Exosomes, Wesley Cox

University of Tennessee Honors Thesis Projects

No abstract provided.


Determining The Effects Of Quercetin On Cadmium Toxicity In Kidney Cells, Elizabeth Dugan 2018 Bellarmine University

Determining The Effects Of Quercetin On Cadmium Toxicity In Kidney Cells, Elizabeth Dugan

Undergraduate Theses

Cadmium is a heavy transition metal that causes kidney disease via prolonged, low-level exposure due to circulating metallothioneins. These proteins transport cadmium ions to the proximal convoluted tubule, where they induce the creation of reactive oxygen species (ROS). Oxidative damage from ROS can lead to kidney dysfunction and eventually failure. Previous studies have shown that antioxidants, including quercetin, which is found in most fruits and vegetables, can lessen cadmium-induced toxicity. In this study, human embryonic kidney cells were pretreated for one hour with varying concentrations of quercetin ranging from 10 - 100 μM. This was followed by 24-hour treatment with 30 ...


Roles Of Phospholipases And Ribosomal S6 Kinase In Lipid Remodeling And Growth In Arabidopsis Response To Phosphate Deprivation, Yuan Su 2018 University of Missouri, St. Louis

Roles Of Phospholipases And Ribosomal S6 Kinase In Lipid Remodeling And Growth In Arabidopsis Response To Phosphate Deprivation, Yuan Su

Dissertations

Phosphate (Pi) is one of three macronutrients for plants, which is vital for plant growth and development. Understanding the mechanism by which plants respond and adapt to Pi deficiency not only unveils functions of genes and pathways involved, but also provides potential tools to manipulate crops to better stand Pi stress in low Pi-containing lands. One of the significant metabolic changes in plants under Pi starvation is the membrane lipid remodeling that converts Pi-containing lipids such as phospholipids to Pi-free lipids, such as glycolipids. To elucidate the metabolism and regulation of lipid remodeling, this dissertation characterizes the role of two ...


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