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Liquid Chromatography Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Elisha Ogbebor 2023 Western Kentucky University

Liquid Chromatography Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Elisha Ogbebor

Masters Theses & Specialist Projects

Bovine mastitis is caused by a wide range of pathogens which results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the innate immune system and act as the first line of defense against mastitis in bovine. β-defensins (~6- 10kDa) are antimicrobial peptides that contain about 28 to 67 amino acids, the presence of six cysteine residues results in their three disulfide bonds and a β-sheet structure. A few β- defensins have been identified in cows based on the site of occurrence and time of expression in tissue, but not in milk. A …


Towards The Quantification Of Estrone And 17Β-Estradiol Conjugates In Dairy Cattle Urine Sorptive Stir Bar Extraction And Gas Chromatography-Mass Spectometry, S.M. Istiak Ahmed 2023 Western Kentucky University

Towards The Quantification Of Estrone And 17Β-Estradiol Conjugates In Dairy Cattle Urine Sorptive Stir Bar Extraction And Gas Chromatography-Mass Spectometry, S.M. Istiak Ahmed

Masters Theses & Specialist Projects

The global concern regarding the presence of compounds with estrogenic properties in the environment has grown significantly. Endocrine-disrupting compounds (EDCs) are environmental pollutants that disrupt the endocrine systems of both wildlife and humans. Estrogens, with their potent estrogenic effects, are particularly noteworthy among the various substances with endocrine-disrupting properties. They are of concern due to their prevalence in the environment, especially due to dairy waste contamination. These lipophilic compounds can accumulate in livestock fat tissues, potentially entering the human food chain. Sulfated forms of estrone and β- estriol, which exhibit a higher degree of saturation than their non-conjugated counterparts, have …


Parrotfish Skull, Grace Mikus, David Kerstetter 2023 Nova Southeastern University

Parrotfish Skull, Grace Mikus, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.


Serranidae Spp. Neurocranium, Justin Abonce, David Kerstetter 2023 Nova Southeastern University

Serranidae Spp. Neurocranium, Justin Abonce, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.


Rainbow Parrotfish Neurocranium, Theophilus J. Hebert, David Kerstetter 2023 Nova Southeastern University

Rainbow Parrotfish Neurocranium, Theophilus J. Hebert, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.


Forages And Technology Management In Growing And Finishing Beef Cattle Systems, Kelton Adair 2023 University of Nebraska-Lincoln

Forages And Technology Management In Growing And Finishing Beef Cattle Systems, Kelton Adair

Department of Animal Science: Dissertations, Theses, and Student Research

A systems study evaluated the effects of an implant [25.7 mg estradiol (Compudose; Elanco Animal Health)] at two rates of gain (LOW: 0.45 kg/d and HIGH: 0.9 kg/d) applied during the winter backgrounding phase in drylot and winter grazing systems and its effects on subsequent phases of production. HIGH supplementation with an implant during the winter backgrounding phase in the drylot system yielded the greatest ending body weight (EBW) during the summer backgrounding phase (P = 0.04). LOW supplementation, without an implant, improved summer backgrounding average daily gain (ADG; P = 0.05) while achieving similar carcass adjusted final body …


Factors Affecting Forage Quality And The Subsequent Response In Production And Energy Metabolism In Lactating Jersey Cows, Kassidy Kate Buse 2023 University of Nebraska-Lincoln

Factors Affecting Forage Quality And The Subsequent Response In Production And Energy Metabolism In Lactating Jersey Cows, Kassidy Kate Buse

Department of Animal Science: Dissertations, Theses, and Student Research

Forage quality is the potential of a forage to produce a production response in animals. Because of how essential they are in dairy cattle rations, forages can have major influence on the behavior and production of dairy cattle. In experiment 1, the effect of forage fragility on the rumen environment and digestibility was evaluated. Increasing the inclusion of a less fragile forage led to greater rumen retention time, which resulted in lower rumen pH and greater NDF digestibility. Experiment 2 evaluated the effects of diets high in either corn silage or alfalfa haylage supplemented with either corn- or soybased protein …


The Influence Of Invasive Species On Fishers’ Satisfactions, Caroline M. LaPlante 2023 University of Nebraska-Lincoln

The Influence Of Invasive Species On Fishers’ Satisfactions, Caroline M. Laplante

School of Natural Resources: Dissertations, Theses, and Student Research

Invasives species are prevalent and widespread in North America. Outdoor recreational activities, such as fishing, introduce a point in which humans may interact with invasive species and have to adapt their own behaviors. Bigheaded carp in the Missouri River below Gavin’s Point Dam are a group of invasive fish species that were thought to be negatively relating to recreational fishers’ satisfactions. Using a content analysis and an importance-grid, we conclude that invasive species do not strongly relate to recreational paddlefish fishers’ satisfactions. Paddlefish fishers represent a small sub-set of recreational fishers in Nebraska and South Dakota. The content analysis revealed …


The Evaluation Of Feed Additives On Reducing Enteric Methane Production From Cattle, Reba L. Colin 2023 University of Nebraska-Lincoln

The Evaluation Of Feed Additives On Reducing Enteric Methane Production From Cattle, Reba L. Colin

Department of Animal Science: Dissertations, Theses, and Student Research

Environmental sustainability can be positively impacted by the inclusion of feed additives to reduce enteric methane production from cattle. Methane production can be affected by feed additives that either alter the rumen environment or act as methanogenesis inhibitors. A reduction in methane from cattle can contribute to meeting carbon neutrality.

A metabolism study was conducted to evaluate Alga 1.0, a product containing bromoform, fed to cattle to evaluate the effects on gas emissions. Treatments were (0, 69, or 103 g/d Alga 1.0) fed in a corn-based diet. Headbox-style indirect calorimeters were used to measure gas emissions. Feeding Alga 1.0 linearly …


Investigation Of Breeding Objectives And Indexes-In-Retrospect, Hunter F. Valasek 2023 University of Nebraska-Lincoln

Investigation Of Breeding Objectives And Indexes-In-Retrospect, Hunter F. Valasek

Department of Animal Science: Dissertations, Theses, and Student Research

This thesis presents two projects that investigate the impacts of breeding objectives and indexes-in-retrospect within the beef cattle industry. The first project synthesized how changes due to planning horizon, breeding system, and sale endpoint in the economic breeding goal of cattle influence the relative emphasis of traits as well as the re-ranking of selection candidates. A total of three breeding systems that represent straightbred and crossbred cattle were used along with six planning horizons (2, 5, 10, 20, 30, and 50 yrs.) for both a weaning and slaughter point of sale. The combination of these different breeding objective attributes resulted …


Predicting Body Weight Of Cattle And Nutrient Digestion Of Individual Sweet Bran Components To Improve Beef Cattle Production Efficiency, Dalton J. Anderson 2023 University of Nebraska-Lincoln

Predicting Body Weight Of Cattle And Nutrient Digestion Of Individual Sweet Bran Components To Improve Beef Cattle Production Efficiency, Dalton J. Anderson

Department of Animal Science: Dissertations, Theses, and Student Research

Efficiency is of high importance to beef cattle producers to ensure that beef cattle are being raised without unnecessary resource use. Two separate experiments were conducted to evaluate potential strategies to improve efficiency in beef cattle production. A metabolism study was conducted to evaluate digestion parameters of individual Sweet Bran components. The objective was to determine the effects of diet components on nutrient digestion and rumen fermentation parameters. The individual components of Sweet Bran (Corn bran, solvent-extracted germ meal, and corn steep liquor) were included at 40% diet dry matter (DM) in a steam-flaked corn (SFC) based diet. A SFC-based …


Understanding Avidities Of Recreational Activities For People Possessing Fishing Licenses And Residing In Urban Environments, Kyle F. Hansen 2023 University of Nebraska-Lincoln

Understanding Avidities Of Recreational Activities For People Possessing Fishing Licenses And Residing In Urban Environments, Kyle F. Hansen

School of Natural Resources: Dissertations, Theses, and Student Research

Recreational fishing is one of the world's most popular pastimes, wherein participation is associated with sociodemographic factors. Even so, fishing license sales are declining in the USA in conjunction with a reduction in rural populations as people move to urban areas. Thus, urban areas are constantly growing in population size, population diversity, and geographic size suggesting a need to understand fishing participation in these growing areas. Natural resource managers often use participation to understand recreationists, yet avidity could provide a new way to understand recreationists. The goal of our study is to understand what sociodemographic factors influence the fishing avidity …


Feed Value And Utilization Of Corn Residue: Implications For Cow Performance And Grazing Strategies, Kaylee E. Wheeler 2023 University of Nebraska-Lincoln

Feed Value And Utilization Of Corn Residue: Implications For Cow Performance And Grazing Strategies, Kaylee E. Wheeler

Department of Animal Science: Dissertations, Theses, and Student Research

Two experiments were conducted to determine the correlation of corn residue feed value and utilization on cow performance and potential grazing strategies. A qualitative observation of corn residue grazing by 13 cattle herds across Nebraska was conducted over two years. Based on producer reported grain yields and stocking rates, it was found that these producers were generally not as aggressive as the recommended 1.2 animal unit months (AUM) for each 6,280 kg/ha (100 U.S. bushels/acre) of harvested grain. They stocked 17 to 101% of the recommendation and on average used 63% of the carrying capacity. Across herds, average body condition …


Assessing The Effects Of The Spring Hunting Season Start Date On Wild Turkey Seasonal Productivity And Hunter Behavior, Joseph Quehl 2023 University of Tennessee, Knoxville

Assessing The Effects Of The Spring Hunting Season Start Date On Wild Turkey Seasonal Productivity And Hunter Behavior, Joseph Quehl

Masters Theses

Many states throughout the Southeast have documented declines in wild turkey (Meleagris gallopavo) harvest and estimates of recruitment in poult-per-hen ratios. Wild turkey populations are driven by seasonal productivity, so the decline in these parameters may indicate a decline in the overall population. One hypothesis as to why we are seeing a reduction in productivity and a potential population decline is that the spring hunting season is disrupting the reproductive cycle by harvesting too many males before they have had the opportunity to breed, or by harvesting dominant males and disrupting the social hierarchy of the flock. Our …


The Sociocultural Significance Of Maine's Oyster Based On Media Coverage Analysis, Syeira Clark 2023 University of Maine - Main

The Sociocultural Significance Of Maine's Oyster Based On Media Coverage Analysis, Syeira Clark

Honors College

Throughout the last decade, the oyster aquaculture industry in Maine has boomed, with 6 million pounds of oysters being harvested in 2021, about the amount that was harvested in 2016 (Cough, 2022). According to the founders of the Maine Oyster Trail, there are over 150 oyster farms currently operating on Maine’s coast, which is about twice as many as there were in 2016 (Maine Sea Grant). With this boom in the industry has come a wave of opportunity, as well as news coverage about the shellfish. This study examines the socio-cultural significance to the state of Maine. While the oyster …


Renovating The Ipmu Via Internet Of Things For Pollutant Emission Estimations In Poultry Facilities, Joshua Dotto 2023 University of Nebraska-Lincoln

Renovating The Ipmu Via Internet Of Things For Pollutant Emission Estimations In Poultry Facilities, Joshua Dotto

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The emissions of ammonia (NH3), particulate matter (PM2.5), and carbon dioxide (CO2) are major concerns in poultry facilities. They can pose environmental concerns and nuances. Robust and affordable measurement systems are needed to accurately measure in-barn concentrations and quantify the emissions.

The Intelligent Portable Monitoring Unit (iPMU or PMU3) developed in 2016 was reconstructed into PMU4 to include upgraded NH3 and PM2.5 sensors and wireless connectivity for a low-cost, robust, and accurate air quality monitoring device with contactless data transfer using the concept of Internet of Things (IoT). In addition, a user-friendly …


Assessing The Morphological And Physiological Traits Of Smooth Brome Pastures Under Long Term Grazing And Nutrient Enrichment In Eastern Nebraska, Hassan Shehab 2023 University of Nebraska-Lincoln

Assessing The Morphological And Physiological Traits Of Smooth Brome Pastures Under Long Term Grazing And Nutrient Enrichment In Eastern Nebraska, Hassan Shehab

School of Natural Resources: Dissertations, Theses, and Student Research

Climate change together with the increase in demands for food, feed, fuel, and fiber are becoming a threat to sustainability and resilience of agriculture and pasture lands. Bromus inermis (smooth bromegrass) dominates pastures for cattle grazing in Eastern Nebraska, US, where it is planted in monocultures, and is considered high quality forage because of its palatability and high nutritional value for cattle, especially under intensive management practices. Sustainable management of these pastures is key to long-term resilience. This study aims to assess the performance of smooth bromegrass pastures to the combined effects of long-term management practices (since 2005) of fertilization …


The Impact Of Silage Inclusion In Diets With Different Corn Processing And The Effect Of Natural And Conventional Feeding Programs On Finishing Cattle Performance, Jessica L. Miller 2023 University of Nebraska-Lincoln

The Impact Of Silage Inclusion In Diets With Different Corn Processing And The Effect Of Natural And Conventional Feeding Programs On Finishing Cattle Performance, Jessica L. Miller

Department of Animal Science: Dissertations, Theses, and Student Research

A finishing experiment was conducted to determine the effects of natural and conventional feeding programs on finishing performance of steers and heifers. Factors were conventional and natural feeding programs in steers and heifers. Cattle assigned to the conventional program were fed diets that contained Rumensin (Elanco Animal Health), Tylan (Elanco Animal Health), MGA (Zoetis), and received growth promoting implants whereas cattle in the natural program received neither. Compared to natural fed cattle, G:F was improved by 15.5% when heifers were fed in a conventional program (P < 0.01) and 23.8% when steers were fed in a conventional program (P < 0.01). The incorporation of implants and feed additives within the finishing period increased …


Early-Life Supplementation Of Omega-3 Polyunsaturated Fatty Acids Improved Growth And Skeletal Muscle Glucose Metabolism In The Heat Stress-Induced Iugr Neonatal Lamb, Melanie Ryann White 2023 University of Nebraska-Lincoln

Early-Life Supplementation Of Omega-3 Polyunsaturated Fatty Acids Improved Growth And Skeletal Muscle Glucose Metabolism In The Heat Stress-Induced Iugr Neonatal Lamb, Melanie Ryann White

Department of Animal Science: Dissertations, Theses, and Student Research

Our 1st study determined whether metabolic deficits of IUGR-born neonatal lambs can be improved by ameliorating elevated inflammatory tone. Supplementation with ω-3 polyunsaturated fatty acids (ω-3 PUFA) improved hindlimb insulin-stimulated glucose oxidation rates and basal lactate secretion. Ex vivo skeletal muscle-specific glucose oxidation rates were improved when heightened inflammatory tone was moderated. Pancreatic β cell function in response to hyperglycemia was improved by ω-3 PUFA supplementation. Tempered inflammatory tone also partially recovered skeletal muscle glycogen stores. Thus, we found that targeting heightened inflammatory tone inherent in IUGR-born neonatal lambs moderately improved glucose metabolism and pancreatic β cell …


State Of The Fisheries: Status Reports And Aquatic Resources Of Western Australia 2022/23, S.J. Newman, K.G. Santoro, D.J. Gaughan 2023 Department of Primary Industries and Regional Development, Western Australia

State Of The Fisheries: Status Reports And Aquatic Resources Of Western Australia 2022/23, S.J. Newman, K.G. Santoro, D.J. Gaughan

Status reports of the fisheries and aquatic resources

Aquatic resources within Western Australia (WA) are in good condition, and this has positioned WA as a global leader in sustainable fisheries management. The sustainable fisheries of WA continue to support our strong economy and regional communities. Nonetheless, the lack of a consistent approach to build in the knowledge of Traditional Owners remains a gap in our longer term fisheries science in Western Australia.

Climate change and climate variability continues to impact fish stocks, challenging our ability to effectively monitor, assess, and manage fish stocks. We are continually working with our stakeholders, and the broader community to be adaptive, responsive, …


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