Other Animal Sciences Commons^™

The Ma-10 Cell Line As A Model Of Insl3 Regulation And Leydig Cell Function, Mary E. Strong

Master's Theses

Leydig cells produce testosterone in response to luteinizing hormone (LH) via the cyclic adenosine monophosphate (cAMP)/protein kinase A pathway. Additionally, these cells are responsible for producing insulin-like peptide 3 (INSL3), a peptide hormone that is essential for testicular descent. The insl3 promoter in Leydig cells can be activated by cAMP through the transcription factor Nur77, which has also been shown to regulate the promoters of the steroidogenic enzymes, cyp17 and 3b-hsd. While the mechanism of LH action on testosterone production is well characterized, the effect of LH on insl3 abundance has yet to be shown directly. The MA-10 …

Personality Traits Predict Hierarchy Rank In Male Rainbowfish Social Groups, Mathieu Colléter, Culum Brown

Sentience Collection

Personality traits are becoming increasingly important in explaining adaptive individual differences in animal behaviour and probably represent a leading edge of the evolutionary process. Despite the newfound interest in animal personality among behavioural ecologists, fewstudies have investigated the link between personality traits and fitness measures. We examined this link using male rainbowfish, Melanotaenia duboulayi, as a model species and found that a range of personality traits (aggression, activity and boldness) covaried with a male’s position in a hierarchy, which is directly related to reproductive success in this and many other species. Dominant fish were more aggressive, active, bold and also …

Expression Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Non-Structural Protein 12 In Escherichia Coli, Ariann Boylan

Honors Scholar Theses

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is single-stranded, positive-sense RNA virus in the family Arteviridae, order Nidovirales. PRRSV began circulating in the U.S swine population in the 1980's and since then becoming the most economically significant disease of swine herds. PRRSV is now disseminated worldwide causing great economic losses to pork industries. PRRSV single-stranded RNA genome is15 kb in length. The genome encodes 9 open reading frames (ORF1a, ORF1b,

ORF2a, ORF2b and ORFs 3 through 7). ORFs 1a and 1b encode for 13 non-structural proteins (nsp) that are suggested to be involved in transcription and viral genome replication. The …

Heartworm In Canines: Trends In Rhode Island Shelter Populations, Kelsey L. Mckenna

Senior Honors Projects

Heartworm in Canines

Kelsey McKenna

Faculty Sponsor: Darlene Jones, DVM, Animal Science

Each year thousands of dogs in the United States become infected with heartworm, which is caused by Dirofilaria immitis. D. immitis is a parasite that is transmitted to dogs via a bite by an infected mosquito. Nine of the seventy competent mosquito species reside in Rhode Island. The true incidence of D. immitis is unknown. Current incidence maps are derived from data generated at veterinary clinics and do not include shelter dogs. The objective of this study is twofold: to determine the prevalence of D. immitis in …

The Effects Of Multispecies Grazing On Pasture Management And Utilization, Chelsea Williams

Masters Theses & Specialist Projects

Multispecies grazing research was conducted using meat-type goats (Capra hircus L.) and Jersey heifers (Bos taurus L.) to determine the relationships between multiple grazing treatments and pasture utilization. The study was conducted for 60 days on the Western Kentucky University Farm in Bowling Green, KY. Cattle and goats have shown to differ in grazing preferences and to be economically important to the area. Grazing treatments included goats and cattle grazing simultaneously, sequentially, and goats grazing alone. A typical established Kentucky pasture was utilized with no weed management practices employed. Predominant forage species included tall fescue (Festuca arundinacea Schreb.), orchardgrass (Dactylis …

Assessing Anglers Identification Of Common Fish Species Of Nebraska, Jason Reed

Department of Environmental Studies: Undergraduate Student Theses

Creel surveys, also known as angler surveys, entail individual interviews with anglers. The interviews include a variety of questions pertaining to their fishing trip on that particular day. The interviewer asks the angler questions that include, but are not limited to what species they caught that day, the size of the fish, how many hours they spent fishing that day, what bait they were using, etc. If the angler does not know the species caught or misidentifies the species there is the potential for the recorded data to negatively impact management techniques that rely on the creel survey data. One …

An Assessment Of Stable Hydrogen-Isotope Analysis Methods To Assign Geographic Origin To Migratory Red-Tailed Hawks (Buteo Jamaicensis), Carla Marie Ahlschwede

Department of Environmental Studies: Undergraduate Student Theses

Stable-hydrogen isotopes are becoming an increasingly popular method of studying migratory birds, though sample preparation methods may affect results. In this study I examined feathers from red-tailed hawks (Buteo jamaicensis) to determine the relationship between measure of δD due to inter-feather variation or drying methods, assessed the accuracy of results by using two birds of known-origin and estimated possible natal origins of migratory red-tailed hawks. Two feathers per individual were taken from 81 wild hawks caught at Hitchcock Nature Center near Crescent IA and from 2 rescued red-tailed hawks, Raptor Recovery Nebraska near Eagle, NE. 119 of the …

Sensory Evaluations Of Usda Select Strip Loin Steaks Enhanced With Sodium And Potassium Phosphates And Usda Choice Strip Loin Steaks For Comparable Palatability Factors, Lindsey Reynolds

Masters Theses & Specialist Projects

The cyclic nature of the beef industry is dependent on the supply and demand transaction initiated by consumers’ acceptability of quality meat products. When purchasing beef at the grocery store, consumers are dependent on USDA grades and visual appraisal; consumers expect consistency in products. Upon this appraisal, quality is determined by the color, marbling content, and texture of a particular retail cut, as well as cooked characteristics of tenderness, flavor, and juiciness. Variability in meat tenderness is a major quality defect of beef (Morgan et al., 1991b; Smith et al., 1992). A common practice used by beef processors to extend …

Replacement Alternatives For Beef Cow Herds: An Analysis Of Retaining Non-Pregnant Cows, Trenton T. Bohling

Department of Agricultural Economics: Dissertations, Theses, and Student Research

A non-pregnant cow is a liability to a producer. Over the last four years, cow-calf producers have had an increased number of non-pregnant cows due to factors like environmental conditions and diseases like trichomoniasis. While most research has indicated that culling a non-pregnant female and replacing the cow with retained heifers, purchased heifers, or purchased cows are the only economic alternatives, recent trends in the cattle market have suggested that keeping a non-pregnant cow may also be an alternative.

Annual beef cow budgets were created based on typical Nebraska Sandhills conditions. Revenues and costs in these budgets vary according to …

Analysis Of Long-Term Selection (28 Generations) For Reproduction, Growth, And Carcass Traits In Swine, Wan-Ling Hsu

Department of Animal Science: Dissertations, Theses, and Student Research

The objectives were 1) to estimate responses from 28 generations of selection for increased pigs born alive (BA) with additional selection for increased 180 d weight (WT) and longissimus muscle area (LMA) and decreased backfat (BF) in the last eight generations, 2) to estimate genetic parameters for an array of traits, and 3) to investigate whether a plateau in response for BA has occurred. All lines were derived from the same Large White/Landrace composite population. Index selection for ovulation rate and embryo survival (G0-11) was initiated in L2 in 1981; L1 was selected randomly control line for L2 (G0-23). L2 …

North American Pollinator Partnership Conference: Public Lands Task Force, Tammy Horn

Tammy Horn

No abstract provided.

Molecular Correlates Of Social Dominance: A Novel Role For Ependymin In Aggression, Lynne U. Sneddon, Rupert Schmidt, Yongxiang Fang, Andrew R. Cossins

Ethology Collection

Theoretical and empirical studies have sought to explain the formation and maintenance of social relationships within groups. The resulting dominance hierarchies have significant fitness and survival consequences dependent upon social status. We hypothesised that each position or rank within a group has a distinctive brain gene expression profile that correlates with behavioural phenotype. Furthermore, transitions in rank position should determine which genes shift in expression concurrent with the new dominance status. We used a custom cDNA microarray to profile brain transcript expression in a model species, the rainbow trout, which forms tractable linear hierarchies. Dominant, subdominant and submissive individuals had …

North American Pollinator Partnership Conference: Making A Difference One Pollinator At A Time, Tammy Horn

Tammy Horn

The North American Pollinator Protection Campaign 10th Anniversary conference, held in Washington DC 2010, is the last place I saw myself being invited to a couple of years ago. Unemployed and changing careers, I withdrew from conventional academe to work bees on surface mine sites in Kentucky, which are not conventional places to define new careers.

W256 Composting Small Ruminants In Tennessee, Ricky Skillington

Animals/Livestock

Version 3.0

Three Rs Approaches In The Production And Quality Control Of Fish Vaccines, Paul J. Midtlyng, Coenraad F.M. Hendriksen, Elisabeth Balks, Lukas Bruckner, Lawrence Elsken, Øystein Evensen, Kjetil Fyrand, Allison Guy, Marlies Halder, Penny Hawkins, Gunn Kisen, Anne Berit Romstad, Kira Salonius, Patrick Smith, Lynne U. Sneddon

Veterinary Science and Medicine Collection

The workshop on Three Rs Approaches in the Production and Quality Control of Fish Vaccines aimed a) to identify animal tests currently stipulated for the production and quality control of fish vaccines and to highlight animal welfare concerns associated with these tests; b) to identify viable options to replace, reduce, and refine animal use for fish vaccine testing; and c) to discuss the way forward and set out how the Three Rs may be implemented without jeopardizing the quality of the vaccines. The workshop participants -- experts from academia, regulatory authorities, a scientific animal welfare organization, and the fish vaccine …

Avian Ecology And Conservation In Tropical Agricultural Landscapes With Emphasis On Vermivora Chrysoptera, Richard Brooks Chandler

Open Access Dissertations

The world's biodiversity is concentrated in tropical ecosystems, yet tropical forests are being converted for agriculture at a rapid rate. I evaluated the potential of an alternative coffee production system known as Integrated Open Canopy (IOC) to contribute to avian conservation. This study was conducted from 2005-2010 in the Cordillera de Tilarán, Costa Rica. My results indicate that species richness of forest-dependent birds was higher in IOC farms than in shade coffee farms, and was comparable to secondary forest sites. There was no difference in species richness of Neotropical-Nearctic migrants between IOC and shade coffee farms. Overall similarity was higher …

The Role Of T-Box (Tbx) 2 In Osteoblast Function, Nidhish Francis

Master's Theses

No abstract provided.

In Vivo Investigations Of Polymer Conjugates As Therapeutics, Elizabeth M. Henchey

Masters Theses 1911 - February 2014

Polymer conjugates offer a way to introduce materials into the body that would normally be rejected or cause toxicity. Two polymers are investigated in vivo for uses in chemotherapeutic delivery, protein therapeutics, and DNA transfection. A novel polymer, polyMPC, has the ability to increase doxorubicin loading and its solubility, and is conjugated in a way to release its payload in a low pH environment. Through its conjugation, blood clearance time of doxorubicin is increased, and thus tumor exposure to the drug is increased with a single administration. It can be administered at ten times the concentration of free doxorubicin, and …

A Multidisciplinary Approach To Food Safety Evaluation: Hummus Spoilage And Microbial Analysis Of Kitchen Surfaces In Residential Child Care Institutions (Rcci) In Massachusetts, U.S.A., Elsina E. Hagan

Masters Theses 1911 - February 2014

Food borne illnesses continues to be a public health challenge in the United States (U.S.); an estimated 9.4 million incident cases occurred in 2011. In view of this challenge we conducted two food safety studies; 1) related to product formulation (hummus spoilage challenge study) and 2) evaluating the microbial safety of domestic kitchen surfaces in Residential Child Care Institutions (RCCI pilot study).

Hummus is of Mediterranean origin but is currently eaten globally. This challenge study evaluates a variety of industrial hummus formulations (four in total, differing in pH and/or addition of a preservative (natamycin). Two batches were setup: batch 1; …

Caffeine Metabolism And Cytochrome P450 Enzyme Mrna Expression Levels Of Genetically Diverse Inbred Mouse Strains, Neal Addicott, Michael Malfatti, Gabriela G. Loots

STAR Program Research Presentations

Cytochrome P450 (CYP) enzymes activate or eliminate medications and other xenobiotics, and Caffeine has been used as an indicator of activity for some CYPs. The natural genetic variation in the human population for caffeine metabolizing CYPs does not yet have an established animal model for comparison, but the natural variation in inbred mouse strains presents an attractive starting point. The project gave four different strains of mice a 40mg/kg dose of caffeine and sampled blood and liver tissue after 30 minutes. Samples were analyzed using High Performance Liquid Chromatography (HPLC), reverse transcription PCR (rtPCR), and quantitative PCR (qPCR). The DBA/2J …