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Lab-Scale Assessment Of Gaseous Emissions From Laying-Hen Manure Storage As Affected By Physical And Environmental Factors, Hong Li, Hongwei Xin 2010 Iowa State University

Lab-Scale Assessment Of Gaseous Emissions From Laying-Hen Manure Storage As Affected By Physical And Environmental Factors, Hong Li, Hongwei Xin

Agricultural and Biosystems Engineering Publications

Manure-belt (MB) and high-rise (HR) laying-hen houses are the two predominant housing types in the U.S. Compared with HR houses, MB houses have better indoor air quality and lower aerial emissions as a result of frequent (every 1 to 4 d) manure removal from the hen houses into separate manure storage. However, emissions from on-farm manure storage are integral parts of the whole-farm emissions and need to be quantified. This series of lab-scale studies assesses emission rates (ER) of ammonia (NH3) and greenhouse gases (CH4, CO2, and N2O) from stored laying-hen manure as affected by the following physical and ...


Livestock Grazing And Vegetative Filter Strip Buffer Effects On Runoff Sediment, Nitrate, And Phosphorus Losses, David F. Webber, Steven K. Mickelson, Syed I. Ahmed, James R. Russell, Wendy J. Powers, Richard C. Schultz, John Kovar 2010 Iowa State University

Livestock Grazing And Vegetative Filter Strip Buffer Effects On Runoff Sediment, Nitrate, And Phosphorus Losses, David F. Webber, Steven K. Mickelson, Syed I. Ahmed, James R. Russell, Wendy J. Powers, Richard C. Schultz, John Kovar

Agricultural and Biosystems Engineering Publications

Livestock grazing in the Midwestern United States can result in significant levels of runoff sediment and nutrient losses to surface water resources. Some of these contaminants can increase stream eutrophication and are suspected of contributing to hypoxic conditions in the Gulf of Mexico. This research quantified effects of livestock grazing management practices and vegetative filter strip buffers on runoff depth and mass losses of total solids, nitrate-nitrogen (NO3-N), and ortho-phosphorus (PO4-P) under natural hydrologic conditions. Runoff data were collected from 12 rainfall events during 2001 to 2003 at an Iowa State University research farm in central ...


Comparison Of The Iowa State University Effluent Limitation Guidelines Model With The Soil-Plant-Air-Water Model For Evaluating Containment Basin Performance, Daniel S. Andersen, Robert T. Burns, Lara B. Moody, Matthew J. Helmers, Robert Horton Jr. 2010 Iowa State University

Comparison Of The Iowa State University Effluent Limitation Guidelines Model With The Soil-Plant-Air-Water Model For Evaluating Containment Basin Performance, Daniel S. Andersen, Robert T. Burns, Lara B. Moody, Matthew J. Helmers, Robert Horton Jr.

Agricultural and Biosystems Engineering Publications

This article compares results from the Iowa State University Effluent Limitations Guidelines (ISU-ELG) model to results obtained using the Soil-Plant-Air-Water (SPAW) model to simulate feedlot runoff containment basin overflow volume. The objective was to verify that the ISU-ELG model was providing a reasonable prediction of basin overflow. The ISU-ELG model uses a set of guidelines to determine if land application is acceptable, whereas the SPAW model uses a soil moisture criterion. The criterion for determining if a particular day was suitable for land application of basin effluent was investigated to determine the effect on basin overflow volumes. The results show ...


Technical Note: Hydraulic Property Determination Of Denitrifying Bioreactor Fill Media, Laura E. Christianson, Adrià Castelló, Reid D. Christianson, Matthew J. Helmers, Alok Bhandari 2010 Iowa State University

Technical Note: Hydraulic Property Determination Of Denitrifying Bioreactor Fill Media, Laura E. Christianson, Adrià Castelló, Reid D. Christianson, Matthew J. Helmers, Alok Bhandari

Agricultural and Biosystems Engineering Publications

Denitrification bioreactors are one of the newest options for nitrate removal in agricultural drainage waters. Optimization of denitrification bioreactor design requires the ability to identify concrete values for the hydraulic properties of bioreactor fill media. Hydraulic properties, chiefly saturated hydraulic conductivity but also porosity and particle size, are not known for many types of possible bioreactor media though they have a significant impact upon bioreactor design and performance. This work was undertaken to more fully quantify the hydraulic properties of the major type of fill media used in Iowa denitrification bioreactors through a series of porosity, hydraulic conductivity, and particle ...


Effect Of Ultrasonic Pretreatment On Methane Production Potential From Corn Ethanol Coproducts, Wei Wu-Haan, Robert T. Burns, Lara B. Moody, Cody John Hearn, David A. Grewell 2010 Iowa State University

Effect Of Ultrasonic Pretreatment On Methane Production Potential From Corn Ethanol Coproducts, Wei Wu-Haan, Robert T. Burns, Lara B. Moody, Cody John Hearn, David A. Grewell

Agricultural and Biosystems Engineering Publications

This article addresses the biochemical methane potential (BMP) production from anaerobic digestion of corn-ethanol coproducts including dried distiller grain with solubles (DDGS), distiller's wet grains (DWG), thin stillage, and condensed distiller's solubles (CDS) as well as evaluating the effects of ultrasonic pretreatment on methane production from these feedstocks. Ultrasonic pretreatment was applied with three amplitude settings of 33% (52.8 µmpp), 66% (105.6 µmpp), and 100% (160 µmpp) as well as five time settings (10, 20, 30, 40, and 50 s) to each of the four coproducts prior to conducting benchtop BMP trials. Ultrasonic pretreatment reduced mean ...


Prediction Of Indoor Climate And Long-Term Air Quality Using The Bta-Aqp Model: Part Ii. Overall Model Evaluation And Application, Gang Sun, Steven J. Hoff 2010 Iowa State University

Prediction Of Indoor Climate And Long-Term Air Quality Using The Bta-Aqp Model: Part Ii. Overall Model Evaluation And Application, Gang Sun, Steven J. Hoff

Agricultural and Biosystems Engineering Publications

The objective of this research was to develop a building thermal analysis and air quality predictive (BTA-AQP) model to predict indoor climate and long-term air quality (NH3, H2S, and CO2 concentrations and emissions) for swine deep-pit buildings. This article presents part II of this research, in which the performance of the BTA-AQP model is evaluated using typical meteorological year (TMY3) data in predicting long-term air quality trends. The good model performance ratings (MAE/SD < 0.5, CRM ˜ 0; IoA ˜ 1; and NSEF > 0.5 for all the predicted parameters) and the graphical presentations reveal that the BTA-AQP model was able to accurately forecast indoor climate and gas concentrations ...


Prediction Of Indoor Climate And Long-Term Air Quality Using The Bta-Aqp Model: Part I. Bta Model Development And Evaluation, Gang Sun, Steven J. Hoff 2010 Iowa State University

Prediction Of Indoor Climate And Long-Term Air Quality Using The Bta-Aqp Model: Part I. Bta Model Development And Evaluation, Gang Sun, Steven J. Hoff

Agricultural and Biosystems Engineering Publications

The objective of this research was to develop a building thermal analysis and air quality predictive (BTA-AQP) model to predict ventilation rate, indoor temperature, and long-term air quality (NH3, H2S, and CO2 concentrations and emissions) for swine deep-pit buildings. This article, part I of II, presents a lumped capacitance model (BTA model) to predict the transient behavior of ventilation rate and indoor air temperature according to the thermo-physical properties of a typical swine building, setpoint temperature scheme, fan staging scheme, transient outside temperature, and the heat fluxes from pigs and supplemental heaters. The obtained ventilation rate and resulting indoor air ...


Skeletal Muscle Stem Cells From Animals I. Basic Cell Biology, Michael V. Dodson, Gary J. Hausman, LeLuo Guan, Min Du, Theodore P. Rasmussen, Sylvia P. Poulos, Priya Mir, Werner G. Bergen, Melinda E. Fernyhough, Douglas C. McFarland, Robert P. Rhoads, Beatrice Soret, James M. Reecy, Sandra G. Velleman, Zhihua Jiang 2010 Washington State University

Skeletal Muscle Stem Cells From Animals I. Basic Cell Biology, Michael V. Dodson, Gary J. Hausman, Leluo Guan, Min Du, Theodore P. Rasmussen, Sylvia P. Poulos, Priya Mir, Werner G. Bergen, Melinda E. Fernyhough, Douglas C. Mcfarland, Robert P. Rhoads, Beatrice Soret, James M. Reecy, Sandra G. Velleman, Zhihua Jiang

Animal Science Publications

Skeletal muscle stem cells from food-producing animals are of interest to agricultural life scientists seeking to develop a better understanding of the molecular regulation of lean tissue (skeletal muscle protein hypertrophy) and intramuscular fat (marbling) development. Enhanced understanding of muscle stem cell biology and function is essential for developing technologies and strategies to augment the metabolic efficiency and muscle hypertrophy of growing animals potentially leading to greater efficiency and reduced environmental impacts of animal production, while concomitantly improving product uniformity and consumer acceptance and enjoyment of muscle foods.


Allied Industry Approaches To Alter Intramuscular Fat Content And Composition In Beef Animals, Michael V. Dodson, Zhihua Jiang, Jie Chen, Gary J. Hausman, LeLuo Guan, Jan Novakofski, David P. Thompson, Carol L. Lorenzen, Melinda E. Fernyhough, Priya Mir, James M. Reecy 2010 Washington State University

Allied Industry Approaches To Alter Intramuscular Fat Content And Composition In Beef Animals, Michael V. Dodson, Zhihua Jiang, Jie Chen, Gary J. Hausman, Leluo Guan, Jan Novakofski, David P. Thompson, Carol L. Lorenzen, Melinda E. Fernyhough, Priya Mir, James M. Reecy

Animal Science Publications

Biochemical and biophysical research tools are used to define the developmental dynamics of numerous cell lineages from a variety of tissues relevant to meat quality. With respect to the adipose cell lineage, much of our present understanding of adipogenesis and lipid metabolism was initially determined through the use of these methods, even though the in vitro or molecular environments are far removed from the tissues of meat animals. This concise review focuses on recent cellular and molecular biology-related research with adipocytes, and how the research might be extended to the endpoint of altering red meat quality. Moreover, economic and policy ...


A Calcitonin Receptor (Calcr) Single Nucleotide Polymorphism Is Associated With Growth Performance And Bone Integrity In Response To Dietary Phosphorus Deficiency, L. S. Alexander, A. Qu, Sara A. Cutler, A. Mahajan, Max F. Rothschild, W. Cai, Jack C. Dekkers, Chad H. Stahl 2010 North Carolina State University at Raleigh

A Calcitonin Receptor (Calcr) Single Nucleotide Polymorphism Is Associated With Growth Performance And Bone Integrity In Response To Dietary Phosphorus Deficiency, L. S. Alexander, A. Qu, Sara A. Cutler, A. Mahajan, Max F. Rothschild, W. Cai, Jack C. Dekkers, Chad H. Stahl

Animal Science Publications

Although concerns over the environmental impact of excess P in the excreta from pig production and governmental regulations have driven research toward reducing dietary supplementation of P to swine diets for over a decade, recent dramatic increases in feed costs have further motivated researchers to identify means to further reduce dietary P supplementation. We have demonstrated that genetic background impacts P utilization in young pigs and have identified genetic polymorphisms in several target genes related to mineral utilization. In this study, we examined the impact of a SNP in the calcitonin receptor gene (CALCR) on P utilization in growing pigs ...


Effects Of Training And Facilitation Of Farmers In Uganda On Livestock Development, Agatha Ampaire, Max F. Rothschild 2010 Iowa State University

Effects Of Training And Facilitation Of Farmers In Uganda On Livestock Development, Agatha Ampaire, Max F. Rothschild

Animal Science Publications

Development efforts in lower income countries generally aim to improve the income and nutrition of rural farming households. Frequently development programs train farmers and give them livestock so that those farmers in turn train other farmers and pass on the livestock in the form of offspring. The paper examines the effects of training and facilitation of farmers on livestock development by discussing the differences in performance indicators of three farmer groups. The first group received training and support from a development program, the second group received less training and support from the program and the third group did not receive ...


Srebp Pathway Genes As Candidate Markers In Country Ham Production, Benedicte Renaville, Kimberly L. Glenn, Benny E. Mote, Bin Fan, Kenneth J. Stalder, Max F. Rothschild 2010 Università di Udine

Srebp Pathway Genes As Candidate Markers In Country Ham Production, Benedicte Renaville, Kimberly L. Glenn, Benny E. Mote, Bin Fan, Kenneth J. Stalder, Max F. Rothschild

Animal Science Publications

Country hams are dry-cured products from the Southeastern region of the USA. This high value product requires quality fresh meat to avoid later processing problems. The marker SREBF1 is a transcription factor involved in the regulation of fatty acid synthesis and anti-oxidative enzyme transcription. The SREBF1 gene and its regulators, SCAP and MBTPS1, were investigated for associations with several meat quality traits in country hams. After single nucleotide polymorphism (SNP) identification, PCR-RFLP tests were designed for one polymorphism in each of the three investigated genes. Meat quality and physical traits were collected on 299 fresh hams. Significant associations were found ...


Spring Seed Guide 2010: Nebraska Variety And Hybrid Tests -- Soybeans, Teshome Regassa, Robert N. Klein, Bruce Anderson, Charles A. Shapiro, Jim Krall 2010 University of Nebraska-Lincoln

Spring Seed Guide 2010: Nebraska Variety And Hybrid Tests -- Soybeans, Teshome Regassa, Robert N. Klein, Bruce Anderson, Charles A. Shapiro, Jim Krall

Agronomy & Horticulture -- Faculty Publications

NEBRASKA SOYBEAN VARIETY TESTS 2009

Crop Production Summary: The November 2009 estimated soybean yield for Nebraska was 52 bushels per acre from 4.800 million harvested acres. The total production of soybeans for the state was forecasted at 247 million bushels. These estimates are from the November National Agricultural Statistics Service.


Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris, Jer-Young Lin, Robert M. Stupar, Christian Hans, D. L. Hyten, Scott A. Jackson 2010 Purdue University

Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris, Jer-Young Lin, Robert M. Stupar, Christian Hans, D. L. Hyten, Scott A. Jackson

Agronomy & Horticulture -- Faculty Publications

Soybean (Glycine max) has undergone at least two rounds of polyploidization, resulting in a paleopolyploid genome that is a mosaic of homoeologous regions. To determine the structural and functional impact of these duplications, we sequenced two ~1-Mb homoeologous regions of soybean, Gm8 and Gm15, derived from the most recent ~13 million year duplication event and the orthologous region from common bean (Phaseolus vulgaris), Pv5. We observed inversions leading to major structural variation and a bias between the two chromosome segments as Gm15 experienced more gene movement (gene retention rate of 81% in Gm15 versus 91% in Gm8) and a nearly ...


Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean, Ki-Seung Kim, Stephanie Bellendir, Karen A. Hudson, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers 2010 University of Illinois

Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean, Ki-Seung Kim, Stephanie Bellendir, Karen A. Hudson, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Agronomy & Horticulture -- Faculty Publications

The soybean aphid (Aphis glycines Matsumura) is an important soybean [Glycine max (L.) Merr.] pest in North America. The dominant aphid resistance gene Rag1 was previously mapped from the cultivar ‘Dowling’ to a 12 cM marker interval on soybean chromosome 7 (formerly linkage group M). The development of additional genetic markers mapping closer to Rag1 was needed to accurately position the gene to improve the effectiveness of markerassisted selection (MAS) and to eventually clone it. The objectives of this study were to identify single nucleotide polymorphisms (SNPs) near Rag1 and to position these SNPs relative to Rag1. To generate a ...


Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers 2010 University of Illinois

Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Agronomy & Horticulture -- Faculty Publications

The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from ‘Dowling’. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping was carried ...


Soydb: A Knowledge Database Of Soybean Transcription Factors, Zheng Wang, Marc Libault, Trupti Joshi, Babu Valliyodan, Henry T. Nguyen, Dong Xu, Gary Stacey, Jianlin Cheng 2010 University of Missouri

Soydb: A Knowledge Database Of Soybean Transcription Factors, Zheng Wang, Marc Libault, Trupti Joshi, Babu Valliyodan, Henry T. Nguyen, Dong Xu, Gary Stacey, Jianlin Cheng

Agronomy & Horticulture -- Faculty Publications

Background: Transcription factors play the crucial rule of regulating gene expression and influence almost all biological processes. Systematically identifying and annotating transcription factors can greatly aid further understanding their functions and mechanisms. In this article, we present SoyDB, a user friendly database containing comprehensive knowledge of soybean transcription factors.

Description: The soybean genome was recently sequenced by the Department of Energy-Joint Genome Institute (DOE-JGI) and is publicly available. Mining of this sequence identified 5,671 soybean genes as putative transcription factors. These genes were comprehensively annotated as an aid to the soybean research community. We developed SoyDB - a knowledge database ...


High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan 2010 USDA, Agricultural Research Service

High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan

Agronomy & Horticulture -- Faculty Publications

Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly. To this end we developed a method which couples sequences obtained from the Roche 454-FLX system (454) with the Illumina Genome Analyzer (GA) for high-throughput SNP discovery. Results: Using a multi-tier reduced representation library we discovered a total of 3 ...


Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan 2010 USDA-ARS, Soybean Genomics and Improvement Lab., Beltsville, MD

Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan

Agronomy & Horticulture -- Faculty Publications

Simple sequence repeat (SSR) genetic markers, also referred to as microsatellites, function in map-based cloning and for marker-assisted selection in plant breeding. The objectives of this study were to determine the abundance of SSRs in the soybean genome and to develop and test soybean SSR markers to create a database of locus-specific markers with a high likelihood of polymorphism. A total of 210,990 SSRs with di-, tri-, and tetranucleotide repeats of five or more were identified in the soybean whole genome sequence (WGS) which included 61,458 SSRs consisting of repeat units of di- (≥10), tri- (≥8), and tetranucleotide ...


Crop Registration: The Pathway To Public Access Of Plant Genetic Materials To Build Crops For The Future, D. D. Ellis, K. A. Garland-Campbell, J. A. Grotenhuis, M. M. Jenderek, Jeffrey F. Pedersen 2010 USDA, ARS, National Center for Genetic Resources Preservation

Crop Registration: The Pathway To Public Access Of Plant Genetic Materials To Build Crops For The Future, D. D. Ellis, K. A. Garland-Campbell, J. A. Grotenhuis, M. M. Jenderek, Jeffrey F. Pedersen

Agronomy & Horticulture -- Faculty Publications

Starting in 1926 and continuing for 80+ yr, 11,241 crop genetic materials have been registered as of 31 Dec. 2008. The crop registration process is an important pathway to publically describe and document new and useful genetic materials and to incorporate these into the public domain via the National Plant Germplasm System (NPGS). Crop Registration materials are now searchable via the Germplasm Resources Information Network (GRIN) and demand for registered materials remains strong with more than 9150 registered accessions distributed in the past 26 yr by the NPGS. Guidelines continue to evolve to accommodate global factors effecting germplasm exchange ...


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