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Hplc-Cuprac Post-Column Derivatization Method For The Determination Of Antioxidants: A Performance Comparison Between Porous Silica And Core-Shell Column Packing, Syed A. Haque, Socrates Jose P. Cañete
Hplc-Cuprac Post-Column Derivatization Method For The Determination Of Antioxidants: A Performance Comparison Between Porous Silica And Core-Shell Column Packing, Syed A. Haque, Socrates Jose P. Cañete
Kentucky Tobacco Research and Development Center Faculty Publications
Background: An HPLC method employing a post-column derivatization strategy using the cupric reducing antioxidant capacity reagent (CUPRAC reagent) for the determining antioxidants in plant-based materials leverages the separation capability of regular HPLC approaches while allowing for detection specificity for antioxidants.
Methods: Three different column types, namely core-shell and porous silica including two chemically different core-shell materials (namely phenyl-hexyl and C18), were evaluated to assess potential improvements that could be attained by changing from a porous silica matrix to a core-shell matrix. Tea extracts were used as sample matrices for the evaluation specifically looking at catechin and epigallocatechin gallate (EGCG).
Results: …