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Evaluation Of Dna Probes For Detection Of Shiga-Like-Toxin- Producing Escherichia Coli In Food And Calf Fecal Samples, Mansour Samadpour, John Liston, Jerry E. Ongerth, Phillip I. Tarr

Faculty of Engineering and Information Sciences - Papers: Part A

The use of DNA probes for Shiga-like toxin I (SLT-I) and SLT-II for detection of SLT-producing Escherichia coli (SLTEC) in foods and calf fecal samples was evaluated. Enrichment cultures were prepared from food or fecal samples. Colonies formed by plating of enrichment cultures were probed for SLTEC by colony hybridization. Alternatively, enrichment cultures were analyzed for SLTEC presence by dot blot. The lowest detected concentration of SLTEC in sample homogenates inoculated with E. coli 0157:H7 corresponded to 1.3 CFU/g of sample. Of the 44 food samples and 28 fecal samples from dairy calves tested by the colony hybridization method, 4 …