Physics Commons^™

Enhanced Nanopore Detection Via Diffusion Gradients And Optical Tweezers, Kyle T. Brady

Theses and Dissertations

Nanopore-based resistive pulse sensing represents an important class of single-molecule measurements. It provides information about many molecules of interest (i.e. DNA, proteins, peptides, clusters, polymers, etc.) without the need for labeling. Two experiments that are especially well suited for studying with nanopore sensors are DNA sequencing and DNA-protein force measurements. This thesis will describe progress that has been made in both areas.

DNA sequencing has become an active area of research for stochastic single-molecule sensing, with many researchers striving for the ultimate goal of single-molecule de novo DNA sequencing. One intriguing method towards that goal involves the use of a …

Diffusion Of Rubidium Vapor Through Hollow-Core Fibers For Gas-Phase Fiber Lasers, Eric M. Guild

Theses and Dissertations

This work examines the diffusion of rubidium through a small diameter tube alone and in the presence of noble gases. A fluid dynamics analysis is investigated to aid in choosing a method for transferring atomic rubidium vapor that is reliable and efficient. Solutions to the time dependant ordinary differential equation describing the experimental flow properties of the system reveal more precise outcomes than previously practiced routines. Resolved viscosities and Poiseuille flow theory velocity profile distributions are characterized for noble gas carriers of the rubidium vapor. Applying Reynolds Numbers to the flow experiments provides pressure differential boundaries that are employed in …