Open Access. Powered by Scholars. Published by Universities.®

Physics Commons

Open Access. Powered by Scholars. Published by Universities.®

1993

Iowa State University

Analytic

Articles 1 - 1 of 1

Full-Text Articles in Physics

An Axial Approach To Detection In Capillary Electrophoresis , John Aaron Taylor Jan 1993

An Axial Approach To Detection In Capillary Electrophoresis , John Aaron Taylor

Retrospective Theses and Dissertations

The purpose of this research has been to develop and demonstrate visualization schemes which further the capabilities of capillary electrophoresis instrumentation. Our approach involves on-axis illumination of the compounds inside the capillary detection region and is applied to absorbance and fluorescence detection;Absorbance measurements were accomplished by focussing an incident beam of laser light into one end of the separation capillary. By utilizing signals collected over the entire length of the analyte band, this technique enhances the analytical path length of conventional absorbance detection sixty fold. The demonstrated instrument offers a fifteen-fold improvement in concentration limits of detection;Three fluorescence ...