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Full-Text Articles in Physics

A Simple And Efficient Centrifugation Filtration Method For Bacterial Concentration And Isolation Prior To Testing Liquid Specimens With Laser-Induced Breakdown Spectroscopy, Dylan J. Malenfanta, Alexandra E. Paulick, Steven J. Rehse Aug 2019

A Simple And Efficient Centrifugation Filtration Method For Bacterial Concentration And Isolation Prior To Testing Liquid Specimens With Laser-Induced Breakdown Spectroscopy, Dylan J. Malenfanta, Alexandra E. Paulick, Steven J. Rehse

Physics Publications

An inexpensive filtration device was designed and constructed to rapidly concentrate bacteria in a liquid suspension on the surface of a disposable filter medium while at the same time separating the bacterial cells from larger contaminants in the suspension on the basis of their size. The device consists of a two-stage insert that is held rigidly in a standard tube during bacterial suspension centrifugation. The filters can be easily removed from the insert for subsequent testing with laser-induced breakdown spectroscopy in a process that takes only three minutes. Filter media of 0.45 micron pore size was found to capture approximately …


Concentration Of Bacterial Specimens During Centrifugation Prior To Laser-Induced Breakdown Spectroscopy Analysis, Alexandra E. Paulicka, Dylan J. Malenfanta, Steven J. Rehse Jul 2019

Concentration Of Bacterial Specimens During Centrifugation Prior To Laser-Induced Breakdown Spectroscopy Analysis, Alexandra E. Paulicka, Dylan J. Malenfanta, Steven J. Rehse

Physics Publications

A metal cone device has been designed and fabricated for use with a custom centrifuge tube insert to allow the simple and rapid concentration of bacterial cells in a circular area with a diameter of 1 mm. The device concentrates cells suspended in up to 1 mL of liquid at the center of a highly flat disposable filter medium which can be easily removed from the centrifuge tube insert for subsequent testing with laser-induced breakdown spectroscopy. Two-dimensional elemental mapping of the filter evidenced a high concentration of bacteria on the filter under the location of the cone hole, as well …


A Review Of The Use Of Laser-Induced Breakdown Spectroscopy For Bacterial Classification, Quantification, And Identification, Steven J. Rehse Apr 2019

A Review Of The Use Of Laser-Induced Breakdown Spectroscopy For Bacterial Classification, Quantification, And Identification, Steven J. Rehse

Physics Publications

The use of laser-induced breakdown spectroscopy to determine the elemental composition of bacterial cells has been described in the peer-reviewed literature since 2003. Fifteen years on, significant accomplishments have been reported that have served to clarify and underscore the areas of bacteriological investigation that LIBS is well-suited for as well as the challenges that yet remain to be faced. This review will attempt to summarize the state of the field by surveying the available body of knowledge. The early days of these experiments, roughly from 2003 to 2007, in which many of the most fundamental experiments were initially conducted will …


Helium Tune-Out Wavelength: Gauge Invariance And Retardation Corrections, Gordon W. F. Drake, Jacob Gabriel Manalo, Pei-Pei Zhang, Kenneth George Herbert Baldwin Mar 2019

Helium Tune-Out Wavelength: Gauge Invariance And Retardation Corrections, Gordon W. F. Drake, Jacob Gabriel Manalo, Pei-Pei Zhang, Kenneth George Herbert Baldwin

Physics Publications

The problem of calculating the tune-out wavelength for an atom interacting with a plane electromagnetic wave is formulated as a zero in the Rayleigh scattering cross section, rather than a zero in the dynamic polarizability. Retardation (finite wavelength) corrections are discussed in the velocity gauge, and possible gauge transformations to a length form are investigated. For the special case of S-states, it is shown that a pure length form exists for the leading pxz retardation correction, even though one does not exist in general. The results of high-precision calculations in Hylleraas coordinates are presented for the tune-out wavelength of …