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Full-Text Articles in Physics
An Optical Coherence Microscope For 3-Dimensional Imaging In Developmental Biology, Barbara M. Hoeling, Andrew D. Fernandez, Richard C. Haskell, Eric Huang, Whittier R. Myers, Daniel C. Petersen, Sharon E. Ungersma, Ruye Wang, Mary E. Williams, Scott E. Fraser
An Optical Coherence Microscope For 3-Dimensional Imaging In Developmental Biology, Barbara M. Hoeling, Andrew D. Fernandez, Richard C. Haskell, Eric Huang, Whittier R. Myers, Daniel C. Petersen, Sharon E. Ungersma, Ruye Wang, Mary E. Williams, Scott E. Fraser
All HMC Faculty Publications and Research
An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 mm (FWHM), and the depth resolution is 10 mm (FWHM) in tissue. The design trade-offs for a 3-D OCM are discussed, and the fundamental photon noise limitation is measured and compared with theory. A rotating 3-D image of a frog embryo is presented to illustrate the capabilities of the instrument.