Physics Commons^™

Motion-Sensitive 3-D Optical Coherence Microscope Operating At 1300 Nm For The Visualization Of Early Frog Development, Barbara M. Hoeling, Stephanie S. Feldman, Daniel T. Strenge, Aaron Bernard, Emily R. Hogan, Daniel C. Petersen, Scott E. Fraser, Yun Kee, J. Michael Tyszka, Richard C. Haskell

All HMC Faculty Publications and Research

We present 3-dimensional volume-rendered in vivo images of developing embryos of the African clawed frog Xenopus laevis taken with our new en-face-scanning, focus-tracking OCM system at 1300 nm wavelength. Compared to our older instrument which operates at 850 nm, we measure a decrease in the attenuation coefficient by 33%, leading to a substantial improvement in depth penetration. Both instruments have motion-sensitivity capability. By evaluating the fast Fourier transform of the fringe signal, we can produce simultaneously images displaying the fringe amplitude of the backscattered light and images showing the random Brownian motion of the scatterers. We present time-lapse movies of …