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Open Access Theses & Dissertations

Microscopy

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Super Resolution Pump-Probe Microscopy With Point Spread Function Engineering, Farzaneh Mohajerani Jan 2014

Super Resolution Pump-Probe Microscopy With Point Spread Function Engineering, Farzaneh Mohajerani

Open Access Theses & Dissertations

Since the last decade, new techniques have made optical microscopy break the diffraction barrier of resolution where all of them are based on molecular fluorescence. Among them, stimulated emission depletion microscopy (STED) has reached less than 25 nm resolution by engineering the point spread function. However, the existing obstacles associated with fluorescence tagging makes it desirable to achieve label-free imaging. Recently the pump-probe method has made it possible to obtain image contrast from molecular absorption and vibration signatures that do not depend on fluorescence. We introduce Super Resolution Pump-Probe Microscopy (SRPPM), in which we combine both PSF engineering method and ...


Two-Photon Microscopy Of Nanoparticles And Biotissues, Judith Noemi Rivera Jan 2013

Two-Photon Microscopy Of Nanoparticles And Biotissues, Judith Noemi Rivera

Open Access Theses & Dissertations

Biomedical Imaging is an important tool in medical research and clinical practice. From understanding the fundamental processes involved in our biological makeup to its use in diagnostics in helping determine what ails us, the advancements in imaging and microscopy have helped shape our view of the world and nature. Microscopy in particular is often used to study the smallest of cells and their dynamical properties while attempting to minimally change the sample being studied. My research objective is largely divided into two parts. The first part consists of designing a video-rate raster scanning two-photon microscope that is faster than current ...