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Cafeteria roenbergensis

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Studying Cellular And Molecular Interaction With Two-Photon Fluorescence Microscopy, Arifur Rahaman Jan 2015

Studying Cellular And Molecular Interaction With Two-Photon Fluorescence Microscopy, Arifur Rahaman

Open Access Theses & Dissertations

Advances in microscopy and fluorescent probes provide new insight into studying cellular and molecular interactions. Two-photon fluorescence microscopy is one of the most important recent inventions in cellular and molecular study. This technology enables noninvasive study at cellular and molecular levels in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. In this study, we used our two-photon microscope to observe predatorial behavior of fast moving bacterivorous marine organism Cafeteria roenbergensis and quantify Mycobacterium ...


Integrating Two-Photon Microscopy And Cryo-Electron Microscopy For Studying The Interaction Of Caféteria Roenbergensis And Crov, Seyedmohammadali Aghvami Jan 2014

Integrating Two-Photon Microscopy And Cryo-Electron Microscopy For Studying The Interaction Of Caféteria Roenbergensis And Crov, Seyedmohammadali Aghvami

Open Access Theses & Dissertations

Cafeteria roenbergensis (Cro) is a marine zooplankton; its voracious appetite plays a significant role in regulating bacteria populations. The giant virus that lives within Cro, known as Cafeteria roenbergensis virus (CroV), has an important effect on the mortality of Cro populations. Although viral infections are extremely abundant in oceans, the complete procedure of the infection is still unknown. We study the infection process of Cro by CroV to find out whether the initial contact is through phagocytosis or CroV penetrating the host cell membrane directly. Cro is a moving at speed in the range of 10-100 um/s, therefore, there ...