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Super Resolution Pump-Probe Microscopy With Point Spread Function Engineering, Farzaneh Mohajerani
Super Resolution Pump-Probe Microscopy With Point Spread Function Engineering, Farzaneh Mohajerani
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Since the last decade, new techniques have made optical microscopy break the diffraction barrier of resolution where all of them are based on molecular fluorescence. Among them, stimulated emission depletion microscopy (STED) has reached less than 25 nm resolution by engineering the point spread function. However, the existing obstacles associated with fluorescence tagging makes it desirable to achieve label-free imaging. Recently the pump-probe method has made it possible to obtain image contrast from molecular absorption and vibration signatures that do not depend on fluorescence. We introduce Super Resolution Pump-Probe Microscopy (SRPPM), in which we combine both PSF engineering method and …