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Iowa State University

Series

Chemistry Publications

2012

Nano scale

Articles 1 - 1 of 1

Full-Text Articles in Physics

High-Precision Tracking With Non-Blinking Quantum Dots Resolves Nanoscale Vertical Displacement, Kyle Marchuk, Yijun Guo, Wei Sun, Javier Vela, Ning Fang Mar 2012

High-Precision Tracking With Non-Blinking Quantum Dots Resolves Nanoscale Vertical Displacement, Kyle Marchuk, Yijun Guo, Wei Sun, Javier Vela, Ning Fang

Chemistry Publications

Novel non-blinking quantum dots (NBQDs) were utilized in three-dimensional super-localization, high-precision tracking applications under an automated scanning-angle total internal reflection fluorescence microscope (SA-TIRFM). NBQDs were randomly attached to stationary microtubules along the radial axis under gliding assay conditions. By automatically scanning through a wide range of incident angles with different evanescent-field layer thicknesses, the fluorescence intensity decay curves were obtained. Fit with theoretical decay functions, the absolute vertical positions were determined with sub-10-nm localization precision. The emission intensity profile of the NBQDs attached to kinesin-propelled microtubules was used to resolve the self-rotation of gliding microtubules within a small vertical distance ...