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Iowa State University

Series

Biochemistry, Biophysics and Molecular Biology Publications

2011

Articles 1 - 1 of 1

Full-Text Articles in Physics

Crystal Structure Of The Cusba Heavy-Metal Efflux Complex Of Escherichia Coli, Chih-Chia Su, Feng Long, Michael T. Zimmermann, Kanagalaghatta R. Rajashankar, Robert L. Jernigan, Edward W. Yu Jan 2011

Crystal Structure Of The Cusba Heavy-Metal Efflux Complex Of Escherichia Coli, Chih-Chia Su, Feng Long, Michael T. Zimmermann, Kanagalaghatta R. Rajashankar, Robert L. Jernigan, Edward W. Yu

Biochemistry, Biophysics and Molecular Biology Publications

Gram-negative bacteria, such as Escherichia coli, expel toxic chemicals via tripartite efflux pumps spanning both the inner and outer membranes. The three parts are: 1) a membrane fusion protein connecting 2) a substrate-binding inner membrane transporter to 3) an outer membrane-anchored channel in the periplasmic space. A crystallographic model of this tripartite efflux complex has been unavailable simply because co-crystallization of different components of the system has proven to be extremely difficult. We previously described the crystal structures of both the inner membrane transporter CusA1 and membrane fusion protein CusB2 of the CusCBA efflux system3,4 from ...