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Full-Text Articles in Physics
Endothelial Nitric Oxide Synthase Oxygenase On Lipid Nanodiscs: A Nano-Assembly Reflecting Native-Like Function Of Enos, Ghaith Altawallbeh, Mohammad M. Haque, Kiril A. Streletzky, Dennis J. Stuehr, Mekki Bayachou
Endothelial Nitric Oxide Synthase Oxygenase On Lipid Nanodiscs: A Nano-Assembly Reflecting Native-Like Function Of Enos, Ghaith Altawallbeh, Mohammad M. Haque, Kiril A. Streletzky, Dennis J. Stuehr, Mekki Bayachou
Physics Faculty Publications
© 2017 Elsevier Inc. Endothelial nitric oxide synthase (eNOS) is a membrane-anchored enzyme. To highlight the potential role and effect of membrane phospholipids on the structure and activity of eNOS, we have incorporated the recombinant oxygenase subunit of eNOS into lipid nanodiscs. Two different size distribution modes were detected by multi-angle dynamic light scattering both for empty nanodiscs, and nanodiscs-bound eNOSoxy. The calculated hydrodynamic diameter for mode 1 species was 9.0 nm for empty nanodiscs and 9.8 nm for nanodisc bound eNOSoxy. Spectroscopic Griess assay was used to measure the enzymatic activity. Remarkably, the specific activity of nanodisc-bound eNOSoxy is …
P1: Using Modified Dean Flow Designs To Increase Mixing Performance, Joshua Clark
P1: Using Modified Dean Flow Designs To Increase Mixing Performance, Joshua Clark
Undergraduate Research Posters 2017
We are using numerical solutions for the Navier-Stokes equations and the concentration - diffusion equation to model fluid flow and reactant distribution in serpentine type channels for micromixers/microreactors development. These mixers exploit centripetal forces on the fluid to induce cross-sectional fluid mixing, aka Dean flows. Various modifications are used to increase the mixing character of these crosssectional flows. We found that the performance of these mixers exceeds that of unmodified channels and we currently assess their performance relative to other state of the art methodologies used to induce mixing on the microscale.
P2: Implementation Of Groove Based Designs For Engineering Fluid Flow In Micromixers, Tahir Butt
P2: Implementation Of Groove Based Designs For Engineering Fluid Flow In Micromixers, Tahir Butt
Undergraduate Research Posters 2017
Mixing on microscale is important for the development of miniaturized chemical reactors that use small quantities of reactants and allow better control over the reaction conditions and products. Nevertheless, achieving rapid mixing in this type of micro-reactors is challenging due to the lack of turbulence and slow diffusion on the microscale. In this work we implement micromixers designs based on surface groove/ridge patterns targeted at inducing cross-sectional flows that both extend the interface between the different reactants, as well as induce chaotic advection. We discuss the fabrication of these structures using soft-lithography in PDMS employing a mold and their optical …