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Full-Text Articles in Physics
Modulation Of The Cardiac Calcium Release Channel By Homocysteine Thiolactone, Laura Jean Owen
Modulation Of The Cardiac Calcium Release Channel By Homocysteine Thiolactone, Laura Jean Owen
Dissertations and Theses
Elevated levels in blood serum (≥10μmol/L) of the amino acid homocysteine is strongly correlated with the incidence of heart failure (HF). We present evidence that the cyclic thioester, homocysteine thiolactone (HTL), a metabolic product of homocysteine, irreversibly modifies proteins that regulate the contractile process in cardiac muscle. Two proteins found in the sarcoplasmic reticulum (SR), the Ca2+ pump (SERCA2), and the ryanodine receptor (RyR2), are responsible for controlling the cytosolic Ca2+ concentration and hence the contractile state of the heart. While both improper Ca2+ handling and elevated homocysteine levels have been considered bio-markers in HF, a direct …
Electrokinetic Properties Of Lipid And Sarcoplasmic Reticulum Membranes In Aqueous Electrolyte And In The Presence Of Lipophilic Ions, Laura Elizabeth Satterfield
Electrokinetic Properties Of Lipid And Sarcoplasmic Reticulum Membranes In Aqueous Electrolyte And In The Presence Of Lipophilic Ions, Laura Elizabeth Satterfield
Dissertations and Theses
The purpose of this study is the characterization of the membrane-water interfaces of both sarcoplasmic reticulum membrane (SR) and charged lipid bilayers under varied properties of the surrounding aqueous solution. In this work we studied the electrokinetic properties of liposomes and SR vesicles as well as the interaction of lipophilic ions with these membranes. The study of electrokinetic properties is based on the measurements of electrophoretic mobility of SR membrane vesicles and PC/PG liposomes. Electrophoretic mobility of SR vesicles was measured as a function of ionic strength for six pH values (pH 4.0, 4.7, 5.0, 6.0, 7.5, and 9.0). Electrophoretic …
The Interaction Of Naphthoquinones With The Calcium Release Channel Of Skeletal Muscle Sarcoplasmic Reticulum, Ruohong Xia
The Interaction Of Naphthoquinones With The Calcium Release Channel Of Skeletal Muscle Sarcoplasmic Reticulum, Ruohong Xia
Dissertations and Theses
The sarcoplasmic reticulum (SR) is an intracellular membrane system which regulates cytoplasmic calcium concentration in muscle and controls the contractile state of muscle. In this thesis, the interaction between naphthoquinone and the Ca2+ release mechanism of SR is described. 1,4-naphthoquinone (1,4NQ) is shown to stimulate Ca2+ release and to modify high-affinity ryanodine binding to skeletal muscle sarcoplasmic reticulum. The interaction between 1,4NQ and the SR involves the oxidation ofcritical sulfhydryl groups associated with the Ca2+ release mechanism. The modulation of ryanodine binding by 1,4NQ is biphasic. At low concentrations of 1,4NQ (<10 >μM) ryanodine binding is stimulated, …
Effects Of The Cardioprotective Drugs Dexrazoxane And Adr-925 On Doxorubicin Induced Ca2+-Release From The Sarcoplasmic Reticulum, Thomas Andreas Herzinger
Effects Of The Cardioprotective Drugs Dexrazoxane And Adr-925 On Doxorubicin Induced Ca2+-Release From The Sarcoplasmic Reticulum, Thomas Andreas Herzinger
Dissertations and Theses
The sarcoplasmic reticulum is the intramuscular organelle responsible for the regulation of cytoplasmic calcium levels in muscle. This thesis investigates the effects of the cardioprotective drug, dexrazoxane, and its metabolite ADR-925 on doxorubicin induced calcium release from skeletal sarcoplasmic reticulum. Doxorubicin is a widely used antineoplastic agent. One of the major side effects of doxorubicin usage is chronic cardiotoxicity. Doxorubicin is a potent activator of the calcium release mechanism from the SR. The interaction between doxorubicin and the calcium release channel has been proposed as the possible underlying mechanism behind cardiotoxicity. A short overview of different hypotheses describing doxorubicin induced …
The Interaction Between A Thiol Specific Probe (Opa) And The Single Channel Characteristics Of The Reconstituted Ca++ Release Protein From Skeletal Muscle Sarcoplasmic Reticulum, Alexander Braun
Dissertations and Theses
One advantage of higher life-forms over less developed organisms is their ability to respond to signals from their environment with motion. This requires highly specialized contractile cells and a whole locomotion apparatus. In vertebrates, the cells responsible for movement are the skeletal muscle cells. They receive signals from the autonomic nervous system in the form of an action potential, and they contract in an appropriate manner. Calcium is a vital intracellular passenger whose role in muscular function is to initiate contraction. It is released via specific channel proteins from an internal Ca++ store, the sarcoplasmic reticulum, and triggers muscular contraction, …
O-Phthalaldehyde Modification Of Sarcoplasmic Reticulum Calcium Release, Steffen Koehler
O-Phthalaldehyde Modification Of Sarcoplasmic Reticulum Calcium Release, Steffen Koehler
Dissertations and Theses
Muscle contraction is a phenomena which fascinated already the ancient Greeks. People have long sought to understand the mechanism of muscle contraction. Today we know that in order for muscle to contract, an action potential propagates from the nerve cell to the muscle cell. Upon arriving at the muscle cell, via a mechanism called Excitation- Contraction (E-C) coupling, Ca2 + is released from an intracellular membrane system, the sarcoplasmic reticulum (SR), into the intracellular fluid. The increase of intracellular Ca2 + initiates the interaction between the contractile units which results in force development and tension. The least well understood step …
Proteolytic Modification Of The Ca²-Release Mechanism Of Sarcoplasmic Reticulum In Skeletal Muscle, Ute Goerke
Proteolytic Modification Of The Ca²-Release Mechanism Of Sarcoplasmic Reticulum In Skeletal Muscle, Ute Goerke
Dissertations and Theses
Calcium ions are important mediators in the mechanism of contraction and relaxation of muscle fibers. Depolarization of sarcolemma and transverse tubule causes an increase of myoplasmic ca2+ concentration which induces contraction of the myofibrils. In skeletal muscle fibers, the intracellular Ca2+ concentraton is regulated by an extensive membrane system, the sarcoplasmic reticulum (SR). Ca2+-release from SR is initiated by depolarization of the transverse tubule via a process referred to as excitation-contraction coupling. The Ca2+ - release channel located in the junctional SR plays an important role in this mechanism.