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Articles 1 - 25 of 25
Full-Text Articles in Physics
Towards A Unification Of Supercomputing, Molecular Dynamics Simulation And Experimental Neutron And X-Ray Scattering Techniques, Benjamin Lindner
Towards A Unification Of Supercomputing, Molecular Dynamics Simulation And Experimental Neutron And X-Ray Scattering Techniques, Benjamin Lindner
Doctoral Dissertations
Molecular dynamics simulation has become an essential tool for scientific discovery and investigation. The ability to evaluate every atomic coordinate for each time instant sets it apart from other methodologies, which can only access experimental observables as an outcome of the atomic coordinates. Here, the utility of molecular dynamics is illustrated by investigating the structure and dynamics of fundamental models of cellulose fibers. For that, a highly parallel code has been developed to compute static and dynamical scattering functions efficiently on modern supercomputing architectures. Using state of the art supercomputing facilities, molecular dynamics code and parallelization strategies, this work also …
Investigation Of The Quaternary Structure Of An Abc Transporter In Living Cells Using Spectrally Resolved Resonance Energy Transfer, Deo R. Singh
Theses and Dissertations
Förster resonance energy transfer (FRET) has become an important tool to study proteins inside living cells. It has been used to explore membrane protein folding and dynamics, determine stoichiometry and geometry of protein complexes, and measure the distance between two molecules. In this dissertation, we use a method based on FRET and optical micro-spectroscopy (OptiMiS) technology, developed in our lab, to probe the structure of dynamic (as opposed to static) protein complexes in living cells. We use this method to determine the association stoichiometry and quaternary structure of an ABC transporter in living cells. Specifically, the transporter we investigate originates …
Nanoroughened Surfaces For Efficient Capture Of Circulating Tumor Cells Without Using Capture Antibodies, Weiqiang Chen, Shinuo Weng, Feng Zhang, Steven Allen, Xiang Li, Liwei Bao, Raymond H. W. Lam, Jill A. Macoska, Sofia D. Merajver, Jianping Fu
Nanoroughened Surfaces For Efficient Capture Of Circulating Tumor Cells Without Using Capture Antibodies, Weiqiang Chen, Shinuo Weng, Feng Zhang, Steven Allen, Xiang Li, Liwei Bao, Raymond H. W. Lam, Jill A. Macoska, Sofia D. Merajver, Jianping Fu
Weiqiang Chen
Circulating tumor cells (CTCs) detached from both primary and metastatic lesions represent a potential alternative to invasive biopsies as a source of tumor tissue for the detection, characterization and monitoring of cancers. Here we report a simple yet effective strategy for capturing CTCs without using capture antibodies. Our method uniquely utilized the differential adhesion preference of cancer cells to nanorough surfaces when compared to normal blood cells and thus did not depend on their physical size or surface protein expression, a significant advantage as compared to other existing CTC capture techniques.
Spectral Cross Correlation As A Supervised Approach For The Analysis Of Complex Raman Datasets: The Case Of Nanoparticles In Biological Cells, Mark Keating, Franck Bonnier, Hugh Byrne
Spectral Cross Correlation As A Supervised Approach For The Analysis Of Complex Raman Datasets: The Case Of Nanoparticles In Biological Cells, Mark Keating, Franck Bonnier, Hugh Byrne
Articles
Spectral Cross-correlation is introduced as a methodology to identify the presence and subcellular distribution of nanoparticles in cells. Raman microscopy is employed to spectroscopically image biological cells previously exposed to polystyrene nanoparticles, as a model for the study of nano-bio interactions. The limitations of previously deployed strategies of K-means clustering analysis and principal component analysis are discussed and a novel methodology of Spectral Cross Correlation Analysis is introduced and compared with the performance of Classical Least Squares Analysis, in both unsupervised and supervised modes. The previous study demonstrated the feasibility of using Raman spectroscopy to map cells and identify polystyrene …
Characterization Of Biofilm Formation By Borrelia Burgdorferi In Vitro, Eva Sapi, Scott L. Bastian, Cedric L. Mpoy, Shernea Scott, Amy Rattelle, Namrata Pabbati, Akhila Poruri, Divya Burugu, Priyanka A. Theophilus, Truc V. Pham, Akshita Datar, Navroop K. Dhaliwal, Alan Macdonald, Michael J. Rossi, Saion K. Sinha, David F. Luecke
Characterization Of Biofilm Formation By Borrelia Burgdorferi In Vitro, Eva Sapi, Scott L. Bastian, Cedric L. Mpoy, Shernea Scott, Amy Rattelle, Namrata Pabbati, Akhila Poruri, Divya Burugu, Priyanka A. Theophilus, Truc V. Pham, Akshita Datar, Navroop K. Dhaliwal, Alan Macdonald, Michael J. Rossi, Saion K. Sinha, David F. Luecke
Biology and Environmental Science Faculty Publications
Borrelia burgdorferi, the causative agent of Lyme disease, has long been known to be capable of forming aggregates and colonies. It was recently demonstrated that Borrelia burgdorferi aggregate formation dramatically changes the in vitro response to hostile environments by this pathogen. In this study, we investigated the hypothesis that these aggregates are indeed biofilms, structures whose resistance to unfavorable conditions are well documented. We studied Borrelia burgdorferi for several known hallmark features of biofilm, including structural rearrangements in the aggregates, variations in development on various substrate matrices and secretion of a protective extracellular polymeric substance (EPS) matrix using several modes …
Light Scattering Study Of Elongated Particles: From Inorganic Nanorice To Polypeptide Micelles, Philip Dee
Light Scattering Study Of Elongated Particles: From Inorganic Nanorice To Polypeptide Micelles, Philip Dee
Undergraduate Research Posters 2012
Utilizing the powerful experimental technique of Dynamic Light Scattering (DLS) for size characterization of anisotropic particles can be extremely misleading. Unfortunately, this point is often not realized by researchers who strive for particle sizing of nanoparticles in suspensions. We present a consistent analysis of DDLS results on FeOOH nanorice and outline the potential difficulties and challenges of DDLS application for polypeptide micelles.
Transcendental Thermodynamics, Richard E. Morel, George Fleck
Transcendental Thermodynamics, Richard E. Morel, George Fleck
Kahn Institute Projects
Thermodynamics is often viewed as a narrow, introspective discipline, trapped by its origins in the 18th and 19th centuries. By dramatic contrast, we show that the Fourth Law of Thermodynamics provides explanations and interpretations of all natural events, extending across artificial boundaries of tradition- al academic disciplines. The Fourth Law of Thermodynamics states that far-from-equilibrium systems increase entropy at the maximum rate available to them. This broadly inclusive paradigm applies to systems from molecules, to organisms, to the biosphere. The Fourth Law is the Law of Evolution. All systems that communicate with their environment exhibit self-organization and self-optimization, enabling the …
Szilard Engine Reversibility As Quantum Gate Function, F. Matthew Mihelic
Szilard Engine Reversibility As Quantum Gate Function, F. Matthew Mihelic
Faculty Publications
A quantum gate is a logically and thermodynamically reversible situation that effects a unitary transformation of qubits of superimposed information, and essentially constitutes a situation for a reversible quantum decision. A quantum decision is a symmetry break, and the effect of the function of a Szilard engine is a symmetry break. A quantum gate is a situation in which a reversible quantum decision can be made, and so if a logically and thermodynamically reversible Szilard engine can be theoretically constructed then it would function as a quantum gate. While the traditionally theorized Szilard engine is not thermodynamically reversible, if one …
Challenging Disciplinary Boundaries In The First Year: A New Introductory Integrated Science Course For Stem Majors, Lisa Gentile, Lester Caudill, Mirela Fetea, April L. Hill, Kathy Hoke, Barry Lawson, Ovidiu Z. Lipan, Michael Kerckhove, Carol A. Parish, Krista J. Stenger, Doug Szajda
Challenging Disciplinary Boundaries In The First Year: A New Introductory Integrated Science Course For Stem Majors, Lisa Gentile, Lester Caudill, Mirela Fetea, April L. Hill, Kathy Hoke, Barry Lawson, Ovidiu Z. Lipan, Michael Kerckhove, Carol A. Parish, Krista J. Stenger, Doug Szajda
Department of Math & Statistics Faculty Publications
To help undergraduates make connections among disciplines so they are able to approach, evaluate, and contribute to the solutions of important global problems, our campus has been focused on interdisciplinary research and education opportunities across the science, technology, engineering, and mathematics (STEM) disciplines. This paper describes the mobilization, planning, and implementation of a first-year interdisciplinary course for STEM majors that integrates key concepts found in traditional first-semester biology, chemistry, computer science, mathematics, and physics courses. This team-taught course, Integrated Quantitative Science (IQS), is half of a first-year student’s schedule in both semesters and is composed of a double lecture and …
Challenging Disciplinary Boundaries In The First Year: A New Introductory Integrated Science Course For Stem Majors, Lisa Gentile, Lester Caudill, Mirela Fetea, April L. Hill, Kathy Hoke, Barry Lawson, Ovidiu Z. Lipan, Michael Kerckhove, Carol A. Parish, Krista J. Stenger, Doug Szajda
Challenging Disciplinary Boundaries In The First Year: A New Introductory Integrated Science Course For Stem Majors, Lisa Gentile, Lester Caudill, Mirela Fetea, April L. Hill, Kathy Hoke, Barry Lawson, Ovidiu Z. Lipan, Michael Kerckhove, Carol A. Parish, Krista J. Stenger, Doug Szajda
Biology Faculty Publications
To help undergraduates make connections among disciplines so they are able to approach, evaluate, and contribute to the solutions of important global problems, our campus has been focused on interdisciplinary research and education opportunities across the science, technology, engineering, and mathematics (STEM) disciplines. This paper describes the mobilization, planning, and implementation of a first-year interdisciplinary course for STEM majors that integrates key concepts found in traditional first-semester biology, chemistry, computer science, mathematics, and physics courses. This team-taught course, Integrated Quantitative Science (IQS), is half of a first-year student’s schedule in both semesters and is composed of a double lecture and …
Transport And Spectroscopic Studies Of The Effects Of Fullerene Structure On The Efficiency And Lifetime Of Polythiophene-Based Solar Cells, Emilee L. Sena, Justin H. Peel, Devin Wesenberg, Shreya Nathan, Marianne Wallis, Maxwell J. Giammona, Thorsteinn Adalsteinsson, Brian J. Mcnelis, Richard P. Barber Jr.
Transport And Spectroscopic Studies Of The Effects Of Fullerene Structure On The Efficiency And Lifetime Of Polythiophene-Based Solar Cells, Emilee L. Sena, Justin H. Peel, Devin Wesenberg, Shreya Nathan, Marianne Wallis, Maxwell J. Giammona, Thorsteinn Adalsteinsson, Brian J. Mcnelis, Richard P. Barber Jr.
Physics
Time-dependent measurements of both power conversion efficiency and ultraviolet-visible absorption spectroscopy have been observed for solar cell blends containing the polymer poly(3-hexylthiophene-2,5-diyl) (P3HT) with two different functionalized C60 electron acceptor molecules: commercially available [6,6]-phenyl C61 butyric acid methyl ester (PCBM) or [6,6]-phenyl C61 butyric acid octadecyl ester (PCBOD) produced in this laboratory. Efficiency was found to decay with an exponential time dependence, while spectroscopic features show saturating exponential behavior. Time constants extracted from both types of measurements showed reasonable agreement for samples produced from the same blend. In comparison to the PCBM samples, the stability of the PCBOD blends was …
A Method To Measure The Detective Quantum Efficiency Of Radiographic Systems In Clinical Setting, Michael C. Mcdonald
A Method To Measure The Detective Quantum Efficiency Of Radiographic Systems In Clinical Setting, Michael C. Mcdonald
Electronic Thesis and Dissertation Repository
The risks associated with exposure to radiation make it critical that digital imaging systems give the best possible images for a given dose to the patient. The DQE is the most widely accepted measure of performance and dose efficiency for digital radiography systems, however it is not commonly measured in a clinical environment as part of routine quality assurance. The primary reason for this is that the data provided to the user by clinical systems has typically undergone image processing and therefore may have a non-linear characteristic response. This is a problem because the Fourier metrics of the DQE require …
Nanotopography Influences Adhesion, Spreading, And Self-Renewal Of Human Embryonic Stem Cells, Weiqiang Chen, Luis G. Villa-Diaz, Yubing Sun, Shinuo Weng, Jin Koo Kim, Raymond H. W. Lam, Lin Han, Rong Fan, Paul H. Krebsbach, Jianping Fu
Nanotopography Influences Adhesion, Spreading, And Self-Renewal Of Human Embryonic Stem Cells, Weiqiang Chen, Luis G. Villa-Diaz, Yubing Sun, Shinuo Weng, Jin Koo Kim, Raymond H. W. Lam, Lin Han, Rong Fan, Paul H. Krebsbach, Jianping Fu
Weiqiang Chen
Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type …
Scas Officers And Council Members 2011 - 2012
Scas Officers And Council Members 2011 - 2012
Journal of the South Carolina Academy of Science
No abstract provided.
Quantitative Reagent-Free Detection Of Fibrinogen Levels In Human Blood Plasma Using Raman Spectroscopy, Kelvin Poon, Fiona Lyng, Peter Knief, Orla L. Howe, Aidan Meade, James Curtin, Hugh Byrne, Joseph Vaughan
Quantitative Reagent-Free Detection Of Fibrinogen Levels In Human Blood Plasma Using Raman Spectroscopy, Kelvin Poon, Fiona Lyng, Peter Knief, Orla L. Howe, Aidan Meade, James Curtin, Hugh Byrne, Joseph Vaughan
Articles
Fibrinogen assays are commonly used as part of clinical screening tests to investigate haemorrhagic states, for detection of disseminated intravascular coagulation and as a predictor of a variety of cardiovascular events. The Clauss assay, which measures thrombin clotting time, is the most commonly used method for measuring fibrinogen levels. Nevertheless, inconsistencies are present in inter-manufacturer reagent sources, calibration standards and methodologies. Automated coagulation analysers, which measure changes in optical density during the prothrombin time (PT-Fg), have found use in many hospitals. However, the PT-Fg method is found to give falsely elevated values due to varying choices of calibrants, reagents and …
Capturing Motion, Allison Marsh
Capturing Motion, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Understanding Fracture, Allison Marsh
Understanding Fracture, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Listen, Allison Marsh
Photographic Evidence, Allison Marsh
Photographic Evidence, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Spotlight On Usc: Mechanical Engineering, Allison Marsh
Spotlight On Usc: Mechanical Engineering, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Influencing Art, Allison Marsh
Influencing Art, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Ua94/6/10 Student / Alumni Personal Papers Wku Carl Ellis, Wku Archives
Ua94/6/10 Student / Alumni Personal Papers Wku Carl Ellis, Wku Archives
WKU Archives Collection Inventories
Scientific notebooks used by Carl Ellis during his years at WKU.
Microfluidic Separation Of Live And Dead Yeast Cells Using Reservoir-Based Dielectrophoresis, Saurin Patel, Daniel Showers, Pallavi Vedantam, Tzuen-Rong Tzeng, Shizhi Qian, Xiangchun Xuan
Microfluidic Separation Of Live And Dead Yeast Cells Using Reservoir-Based Dielectrophoresis, Saurin Patel, Daniel Showers, Pallavi Vedantam, Tzuen-Rong Tzeng, Shizhi Qian, Xiangchun Xuan
Mechanical & Aerospace Engineering Faculty Publications
Separating live and dead cells is critical to the diagnosis of early stage diseases and to the efficacy test of drug screening, etc. This work demonstrates a novel microfluidic approach to dielectrophoretic separation of yeast cells by viability. It exploits the cell dielectrophoresis that is induced by the inherent electric field gradient at the reservoir-microchannel junction to selectively trap dead yeast cells and continuously separate them from live ones right inside the reservoir. This approach is therefore termed reservoir-based dielectrophoresis (rDEP). It has unique advantages as compared to existing dielectrophoretic approaches such as the occupation of zero channel space and …
Microfluidic Impedance Spectroscopy As A Tool For Quantitative Biology And Biotechnology, Ahmet C. Sabuncu, Jie Zhuang, Juergen F. Kolb, Ali Beskok
Microfluidic Impedance Spectroscopy As A Tool For Quantitative Biology And Biotechnology, Ahmet C. Sabuncu, Jie Zhuang, Juergen F. Kolb, Ali Beskok
Mechanical & Aerospace Engineering Faculty Publications
A microfluidic device that is able to perform dielectric spectroscopy is developed. The device consists of a measurement chamber that is 250 μm thick and 750 μm radius. Around 1000 cells fit inside the chamber assuming average quantities for cell radius and volume fraction. This number is about 1000 folds lower than the capacity of conventional fixtures. A T-cell leukemia cell line Jurkat is tested using the microfluidic device. Measurements of deionized water and salt solutions are utilized to determine parasitic effects and geometric capacitance of the device. Physical models, including Maxwell-Wagner mixture and double shell models, are used to …
Table Of Contents
Journal of the South Carolina Academy of Science
No abstract provided.