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Full-Text Articles in Physical Sciences and Mathematics
Quantitative Footprinting Analysis. Binding To A Single Site, Robert Rehfuss, Jerry Goodisman, James C. Dabrowiak
Quantitative Footprinting Analysis. Binding To A Single Site, Robert Rehfuss, Jerry Goodisman, James C. Dabrowiak
Chemistry - All Scholarship
The theory for measuring ligand binding constants from footprinting autoradiographic data associated with a single binding site is derived. If the ligand and DNA cleavage agent compete for a common site, the spot intensities are not proportional to the amount of DNA not blocked by ligand. The analysis of a single site is experimentally illustrated by using results for the anticancer drug actinomycin D interacting with the duplex d(TAGCGCTA)2 as probed with the hydrolytic enzyme DNase I.
Synthesis Of Highly-Strained Gem-Difluoro Compounds By Reaction Of Difluoromethylene With Cycloalkenes, Deyi Yang
Theses
Trans-Cyclooctene is thought to be more reactive than cis-cyclooctene due to its trans-structure. When cis or trans-clooctene reacts with difluormethylene (:CF2), trans-9,9-difluorobicyclo[ 6,1,0] nonane was generated in much higher yield than cis-9,9-difluorobicyclo[6,1,0]nonane. [PhCF3P+CF2Br]Br- was chosen to be the difluoromethylene precursor in this study. The reason was that the reaction condition for trans-cyclooctene and :CF2 should be at low temperature. Only [PhCF3P+CF2Br]Br- could generate CF2 at lower temperature (e.g. room temperature). We succeeded in the synthesis of trans-9,9-difluorobicyclo[6,1,0]nonane and its cis isomer and …
Investigation Into Low Cost Flow Injection Analysis, Julian Tyson
Investigation Into Low Cost Flow Injection Analysis, Julian Tyson
Chemistry Department Faculty Publication Series
No abstract provided.
Modern Analytical Chemistry, Julian Tyson
Modern Analytical Chemistry, Julian Tyson
Chemistry Department Faculty Publication Series
No abstract provided.